Schild, Detlev

[["dc.bibliographiccitation.firstpage","1310"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical journal"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","76"],["dc.contributor.author","Engel, J."],["dc.contributor.author","Schultens, H. A."],["dc.contributor.author","Schild, D."],["dc.date.accessioned","2014-02-18T09:32:43Z"],["dc.date.accessioned","2021-10-27T13:20:07Z"],["dc.date.available","2014-02-18T09:32:43Z"],["dc.date.available","2021-10-27T13:20:07Z"],["dc.date.issued","1999-03-01"],["dc.description.abstract","We made a computational model of a single neuron to study the effect of the small conductance (SK) Ca2+-dependent K+ channel on spike frequency adaptation. The model neuron comprised a Na+ conductance, a Ca2+ conductance, and two Ca2+-independent K+ conductances, as well as a small and a large (BK) Ca2+-activated K+ conductance, a Ca2+ pump, and mechanisms for Ca2+ buffering and diffusion. Sustained current injection that simulated synaptic input resulted in a train of action potentials (APs) which in the absence of the SK conductance showed very little adaptation with time. The transfer function of the neuron was nearly linear, i.e., both asymptotic spike rate as well as the intracellular free Ca2+ concentration ([Ca2+]i) were approximately linear functions of the input current. Adding an SK conductance with a steep nonlinear dependence on [Ca2+]i (. Pflügers Arch. 422:223-232; Köhler, Hirschberg, Bond, Kinzie, Marrion, Maylie, and Adelman. 1996. Science. 273:1709-1714) caused a marked time-dependent spike frequency adaptation and changed the transfer function of the neuron from linear to logarithmic. Moreover, the input range the neuron responded to with regular spiking increased by a factor of 2.2. These results can be explained by a shunt of the cell resistance caused by the activation of the SK conductance. It might turn out that the logarithmic relationships between the stimuli of some modalities (e.g., sound or light) and the perception of the stimulus intensity (Fechner's law) have a cellular basis in the involvement of SK conductances in the processing of these stimuli."],["dc.identifier.doi","10.1016/S0006-3495(99)77293-0"],["dc.identifier.fs","1633"],["dc.identifier.pmid","10049314"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9911"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91939"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Action Potentials"],["dc.subject.mesh","Adaptation, Physiological"],["dc.subject.mesh","Biophysical Phenomena"],["dc.subject.mesh","Biophysics"],["dc.subject.mesh","Calcium"],["dc.subject.mesh","Calcium-Transporting ATPases"],["dc.subject.mesh","Computer Simulation"],["dc.subject.mesh","Electric Conductivity"],["dc.subject.mesh","Membrane Potentials"],["dc.subject.mesh","Models, Neurological"],["dc.subject.mesh","Neurons"],["dc.subject.mesh","Potassium Channels"],["dc.title","Small conductance potassium channels cause an activity-dependent spike frequency adaptation and make the transfer function of neurons logarithmic."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","159"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of neuroscience methods"],["dc.bibliographiccitation.lastpage","67"],["dc.bibliographiccitation.volume","121"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Peters, Florian"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2019-07-10T08:14:10Z"],["dc.date.available","2019-07-10T08:14:10Z"],["dc.date.issued","2002-12-15"],["dc.description.abstract","We used a slice preparation of the olfactory epithelium of Xenopus laevis tadpoles to record odorant responses of olfactory receptor neurons (ORNs) and compared these to odorant responses recorded in isolated ORNs. The maximum recording time in the slice was considerably longer than in isolated ORNs, which is essential when many odorants are to be tested. No odorant-induced responses could be obtained from isolated ORNs recorded in the on-cell mode, while recordings in the slice (on-cell and whole-cell) as well as previously reported perforated-patch recordings in isolated ORNs of the same species () were successful, though qualitatively different. In the slice preparation, amino acids as well as an extract from Spirulina algae always induced excitatory responses, while, in a previous study on isolated ORNs, responses were either excitatory or inhibitory. The results of this study show that ORNs obtained using different preparation techniques can give markedly different responses upon the application of odorants. Our experiments indicate that the slice preparation combined with the on-cell configuration of the patch-clamp technique is the method of choice for testing many odorants on individual ORNs."],["dc.identifier.fs","12681"],["dc.identifier.pmid","12468006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9905"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61455"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0165-0270"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Action Potentials"],["dc.subject.mesh","Amino Acids, Acidic"],["dc.subject.mesh","Amino Acids, Aromatic"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Arginine"],["dc.subject.mesh","Cell Separation"],["dc.subject.mesh","Histidine"],["dc.subject.mesh","Lysine"],["dc.subject.mesh","Membrane Potentials"],["dc.subject.mesh","Mucous Membrane"],["dc.subject.mesh","Odors"],["dc.subject.mesh","Olfactory Mucosa"],["dc.subject.mesh","Olfactory Receptor Neurons"],["dc.subject.mesh","Patch-Clamp Techniques"],["dc.subject.mesh","Reproducibility of Results"],["dc.subject.mesh","Stimulation, Chemical"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Odorant responses of Xenopus laevis tadpole olfactory neurons: a comparison between preparations."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]