Schild, Detlev

[["dc.bibliographiccitation.firstpage","1093"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The European journal of neuroscience"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Nezlin, L."],["dc.contributor.author","Rabba, J."],["dc.contributor.author","Müller, B."],["dc.contributor.author","Schild, D."],["dc.date.accessioned","2019-07-10T08:14:11Z"],["dc.date.available","2019-07-10T08:14:11Z"],["dc.date.issued","2001-03-01"],["dc.description.abstract","Norepinephrine (NE) has various modulatory roles in both the peripheral and the central nervous systems. Here we investigate the function of the locus coeruleus efferent fibres in the olfactory bulb of Xenopus laevis tadpoles. In order to distinguish unambiguously between mitral cells and granule cells of the main olfactory bulb and the accessory olfactory bulb, we used a slice preparation. The two neuron types were distinguished on the basis of their location in the slice, their typical branching pattern and by electrophysiological criteria. At NE concentrations lower than 5 microM there was only one effect of NE upon voltage-gated conductances; NE blocked a high-voltage-activated Ca(2+)-current in mitral cells of both the main and the accessory olfactory bulbs. No such effect was observed in granule cells. The effect of NE upon mitral cell Ca(2+)-currents was mimicked by the alpha(2)-receptor agonists clonidine and alpha-methyl-NE. As a second effect, NE or clonidine blocked spontaneous synaptic activity in granule cells of both the main and the accessory olfactory bulbs. NE or clonidine also blocked the spontaneous synaptic activity in mitral cells of either olfactory bulb. The amplitude of glutamate-induced currents in granule cells was modulated neither by clonidine nor by alpha-methyl-NE. Taken together, the main effect of the noradrenergic, presynaptic, alpha(2)-receptor-mediated block of Ca(2)+-currents in mitral cells appeared to be a wide-spread disinhibition of mitral cells in the accessory olfactory bulb as well as in the main olfactory bulb."],["dc.identifier.fs","2568"],["dc.identifier.pmid","11285006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9909"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61458"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0953-816X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Calcium Channel Blockers"],["dc.subject.mesh","Calcium Channels"],["dc.subject.mesh","Clonidine"],["dc.subject.mesh","Electric Conductivity"],["dc.subject.mesh","Larva"],["dc.subject.mesh","Neurons"],["dc.subject.mesh","Norepinephrine"],["dc.subject.mesh","Olfactory Bulb"],["dc.subject.mesh","Synapses"],["dc.subject.mesh","Synaptic Transmission"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Noradrenergic modulation of calcium currents and synaptic transmission in the olfactory bulb of Xenopus laevis tadpoles."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","399"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Chemical senses"],["dc.bibliographiccitation.lastpage","407"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Scheidweiler, U."],["dc.contributor.author","Nezlin, L."],["dc.contributor.author","Rabba, J."],["dc.contributor.author","Müller, B."],["dc.contributor.author","Schild, D."],["dc.date.accessioned","2019-07-10T08:14:10Z"],["dc.date.available","2019-07-10T08:14:10Z"],["dc.date.issued","2001-05-01"],["dc.description.abstract","We report on the development of a slice culture of amphibian brain tissue. In particular, we cultured slices from Xenopus laevis tadpoles that contain the olfactory mucosae, the olfactory nerves, the olfactory bulb and the telencephalon. During 6 days in roller tubes the slices flattened, starting from 250 microm and decreasing to approximately 40 microm, corresponding to about three cell layers. Dendritic processes could be followed over distances as long as 200 microm. Neurons in the cultured slice could be recorded using the patch clamp technique and simultaneously imaged using an inverted laser scanning microscope. We characterized the main neuron types of the olfactory bulb, i.e. mitral cells and granule cells, by correlating their typical morphological features in the acute slice with the electrophysiological properties in both the acute slice and slice culture. This correlation allowed unambiguous identification of mitral cells and granule cells in the slice culture."],["dc.identifier.fs","2567"],["dc.identifier.pmid","11369674"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9907"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61456"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0379-864X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Culture Techniques"],["dc.subject.mesh","Electrophysiology"],["dc.subject.mesh","Larva"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Neurons"],["dc.subject.mesh","Neurons, Afferent"],["dc.subject.mesh","Olfactory Bulb"],["dc.subject.mesh","Patch-Clamp Techniques"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Slice culture of the olfactory bulb of Xenopus laevis tadpoles."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","803"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Chemical senses"],["dc.bibliographiccitation.lastpage","10"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Rössler, Wolfgang"],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Schürmann, Friedrich W."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2019-07-10T08:14:10Z"],["dc.date.available","2019-07-10T08:14:10Z"],["dc.date.issued","2002-11-01"],["dc.description.abstract","Using fluorophore-conjugated phalloidin, we show that filamentous (F)-actin is strongly aggregated in olfactory glomeruli within primary olfactory centers of vertebrates and insects. Our comparative study demonstrates that aggregation of F-actin is a common feature of glomeruli across phyla, and is independent of glomerular architecture and/or the presence or absence of cellular borders around glomeruli formed by neurons or glial cells. The distribution of F-actin in axonal and dendritic compartments within glomeruli, however, appears different between vertebrates and insects. The potential role of the actin-based cytoskeleton in synaptic and structural plasticity within glomeruli is discussed."],["dc.identifier.fs","12683"],["dc.identifier.pmid","12438205"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9908"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61457"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0379-864X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Actins"],["dc.subject.mesh","Ambystoma"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Axons"],["dc.subject.mesh","Dendrites"],["dc.subject.mesh","Goldfish"],["dc.subject.mesh","Immunohistochemistry"],["dc.subject.mesh","Insects"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Olfactory Bulb"],["dc.subject.mesh","Olfactory Pathways"],["dc.subject.mesh","Olfactory Receptor Neurons"],["dc.subject.mesh","Phalloidine"],["dc.subject.mesh","Propidium"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Sense Organs"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Aggregation of f-actin in olfactory glomeruli: a common feature of glomeruli across phyla."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]