Schild, Detlev

[["dc.bibliographiccitation.firstpage","803"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Chemical senses"],["dc.bibliographiccitation.lastpage","10"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Rössler, Wolfgang"],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Schürmann, Friedrich W."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2019-07-10T08:14:10Z"],["dc.date.available","2019-07-10T08:14:10Z"],["dc.date.issued","2002-11-01"],["dc.description.abstract","Using fluorophore-conjugated phalloidin, we show that filamentous (F)-actin is strongly aggregated in olfactory glomeruli within primary olfactory centers of vertebrates and insects. Our comparative study demonstrates that aggregation of F-actin is a common feature of glomeruli across phyla, and is independent of glomerular architecture and/or the presence or absence of cellular borders around glomeruli formed by neurons or glial cells. The distribution of F-actin in axonal and dendritic compartments within glomeruli, however, appears different between vertebrates and insects. The potential role of the actin-based cytoskeleton in synaptic and structural plasticity within glomeruli is discussed."],["dc.identifier.fs","12683"],["dc.identifier.pmid","12438205"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9908"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61457"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0379-864X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Actins"],["dc.subject.mesh","Ambystoma"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Axons"],["dc.subject.mesh","Dendrites"],["dc.subject.mesh","Goldfish"],["dc.subject.mesh","Immunohistochemistry"],["dc.subject.mesh","Insects"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Olfactory Bulb"],["dc.subject.mesh","Olfactory Pathways"],["dc.subject.mesh","Olfactory Receptor Neurons"],["dc.subject.mesh","Phalloidine"],["dc.subject.mesh","Propidium"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Sense Organs"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Aggregation of f-actin in olfactory glomeruli: a common feature of glomeruli across phyla."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","429"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Physiological reviews"],["dc.bibliographiccitation.lastpage","66"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Schild, D."],["dc.contributor.author","Restrepo, D."],["dc.date.accessioned","2019-07-10T08:14:11Z"],["dc.date.available","2019-07-10T08:14:11Z"],["dc.date.issued","1998-04-01"],["dc.description.abstract","Considerable progress has been made in the understanding of transduction mechanisms in olfactory receptor neurons (ORNs) over the last decade. Odorants pass through a mucus interface before binding to odorant receptors (ORs). The molecular structure of many ORs is now known. They belong to the large class of G protein-coupled receptors with seven transmembrane domains. Binding of an odorant to an OR triggers the activation of second messenger cascades. One second messenger pathway in particular has been extensively studied; the receptor activates, via the G protein Golf, an adenylyl cyclase, resulting in an increase in adenosine 3',5'-cyclic monophosphate (cAMP), which elicits opening of cation channels directly gated by cAMP. Under physiological conditions, Ca2+ has the highest permeability through this channel, and the increase in intracellular Ca2+ concentration activates a Cl- current which, owing to an elevated reversal potential for Cl-, depolarizes the olfactory neuron. The receptor potential finally leads to the generation of action potentials conveying the chemosensory information to the olfactory bulb. Although much less studied, other transduction pathways appear to exist, some of which seem to involve the odorant-induced formation of inositol polyphosphates as well as Ca2+ and/or inositol polyphosphate -activated cation channels. In addition, there is evidence for odorant-modulated K+ and Cl- conductances. Finally, in some species, ORNs can be inhibited by certain odorants. This paper presents a comprehensive review of the biophysical and electrophysiological evidence regarding the transduction processes as well as subsequent signal processing and spike generation in ORNs."],["dc.description.abstract","ORN; Ordorants"],["dc.identifier.fs","2147"],["dc.identifier.pmid","9562035"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9910"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61459"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0031-9333"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Olfactory Mucosa"],["dc.subject.mesh","Olfactory Receptor Neurons"],["dc.subject.mesh","Signal Transduction"],["dc.title","Transduction mechanisms in vertebrate olfactory receptor cells."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","159"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of neuroscience methods"],["dc.bibliographiccitation.lastpage","67"],["dc.bibliographiccitation.volume","121"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Peters, Florian"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2019-07-10T08:14:10Z"],["dc.date.available","2019-07-10T08:14:10Z"],["dc.date.issued","2002-12-15"],["dc.description.abstract","We used a slice preparation of the olfactory epithelium of Xenopus laevis tadpoles to record odorant responses of olfactory receptor neurons (ORNs) and compared these to odorant responses recorded in isolated ORNs. The maximum recording time in the slice was considerably longer than in isolated ORNs, which is essential when many odorants are to be tested. No odorant-induced responses could be obtained from isolated ORNs recorded in the on-cell mode, while recordings in the slice (on-cell and whole-cell) as well as previously reported perforated-patch recordings in isolated ORNs of the same species () were successful, though qualitatively different. In the slice preparation, amino acids as well as an extract from Spirulina algae always induced excitatory responses, while, in a previous study on isolated ORNs, responses were either excitatory or inhibitory. The results of this study show that ORNs obtained using different preparation techniques can give markedly different responses upon the application of odorants. Our experiments indicate that the slice preparation combined with the on-cell configuration of the patch-clamp technique is the method of choice for testing many odorants on individual ORNs."],["dc.identifier.fs","12681"],["dc.identifier.pmid","12468006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9905"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61455"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0165-0270"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Action Potentials"],["dc.subject.mesh","Amino Acids, Acidic"],["dc.subject.mesh","Amino Acids, Aromatic"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Arginine"],["dc.subject.mesh","Cell Separation"],["dc.subject.mesh","Histidine"],["dc.subject.mesh","Lysine"],["dc.subject.mesh","Membrane Potentials"],["dc.subject.mesh","Mucous Membrane"],["dc.subject.mesh","Odors"],["dc.subject.mesh","Olfactory Mucosa"],["dc.subject.mesh","Olfactory Receptor Neurons"],["dc.subject.mesh","Patch-Clamp Techniques"],["dc.subject.mesh","Reproducibility of Results"],["dc.subject.mesh","Stimulation, Chemical"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Odorant responses of Xenopus laevis tadpole olfactory neurons: a comparison between preparations."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]