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  • 2017Book Chapter
    [["dc.bibliographiccitation.firstpage","309"],["dc.bibliographiccitation.lastpage","317"],["dc.bibliographiccitation.seriesnr","1650"],["dc.contributor.author","Sydow, Hans-Georg"],["dc.contributor.author","Pieper, Tobias Karl"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Tsikolia, Nikoloz"],["dc.contributor.editor","Sheng, G."],["dc.date.accessioned","2019-11-11T12:47:47Z"],["dc.date.available","2019-11-11T12:47:47Z"],["dc.date.issued","2017"],["dc.description.abstract","Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture."],["dc.identifier.doi","10.1007/978-1-4939-7216-6_21"],["dc.identifier.pmid","28809031"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62594"],["dc.language.iso","en"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.doi","10.1007/978-1-4939-7216-6"],["dc.relation.eissn","1940-6029"],["dc.relation.isbn","978-1-4939-7215-9"],["dc.relation.isbn","978-1-4939-7216-6"],["dc.relation.ispartof","Avian and Reptilian Developmental Biology"],["dc.relation.ispartofseries","Methods in molecular biology;1650"],["dc.relation.issn","1064-3745"],["dc.relation.issn","1940-6029"],["dc.title","An Early Chick Embryo Culture Device for Extended Continuous Observation"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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