Sigrist, Stephan J.

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Fukaya, Ryota"],["dc.contributor.author","Maglione, Marta"],["dc.contributor.author","Sigrist, Stephan J."],["dc.contributor.author","Sakaba, Takeshi"],["dc.date.accessioned","2022-08-19T11:52:31Z"],["dc.date.available","2022-08-19T11:52:31Z"],["dc.date.issued","2021"],["dc.description.abstract","The cyclic adenosine monophosphate (cAMP)-dependent potentiation of neurotransmitter release is important for higher brain functions such as learning and memory. To reveal the underlying mechanisms, we applied paired pre- and postsynaptic recordings from hippocampal mossy fiber-CA3 synapses. Ca2+ uncaging experiments did not reveal changes in the intracellular Ca2+ sensitivity for transmitter release by cAMP, but suggested an increase in the local Ca2+ concentration at the release site, which was much lower than that of other synapses before potentiation. Total internal reflection fluorescence (TIRF) microscopy indicated a clear increase in the local Ca2+ concentration at the release site within 5 to 10 min, suggesting that the increase in local Ca2+ is explained by the simple mechanism of rapid Ca2+ channel accumulation. Consistently, two-dimensional time-gated stimulated emission depletion microscopy (gSTED) microscopy showed an increase in the P/Q-type Ca2+ channel cluster size near the release sites. Taken together, this study suggests a potential mechanism for the cAMP-dependent increase in transmission at hippocampal mossy fiber-CA3 synapses, namely an accumulation of active zone Ca2+ channels."],["dc.identifier.doi","10.1073/pnas.2016754118"],["dc.identifier.pmid","33622791"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113055"],["dc.identifier.url","https://for2705.de/literature/publications/33"],["dc.language.iso","en"],["dc.relation","FOR 2705: Dissection of a Brain Circuit: Structure, Plasticity and Behavioral Function of the Drosophila Mushroom Body"],["dc.relation","FOR 2705 | TP 5: Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.workinggroup","RG Sigrist (Genetics)"],["dc.title","Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","106"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Neurogenetics"],["dc.bibliographiccitation.lastpage","114"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Woitkuhn, Jennifer"],["dc.contributor.author","Ender, Anatoli"],["dc.contributor.author","Beuschel, Christine B."],["dc.contributor.author","Maglione, Marta"],["dc.contributor.author","Matkovic-Rachid, Tanja"],["dc.contributor.author","Huang, Sheng"],["dc.contributor.author","Lehmann, Martin"],["dc.contributor.author","Geiger, Joerg R. P."],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2022-08-19T10:14:13Z"],["dc.date.available","2022-08-19T10:14:13Z"],["dc.date.issued","2020"],["dc.description.abstract","The cellular analysis of mushroom body (MB)-dependent memory forming processes is far advanced, whereas, the molecular and physiological understanding of their synaptic basis lags behind. Recent analysis of the Drosophila olfactory system showed that Unc13A, a member of the M(Unc13) release factor family, promotes a phasic, high release probability component, while Unc13B supports a slower tonic release component, reflecting their different nanoscopic positioning within individual active zones. We here use STED super-resolution microscopy of MB lobe synapses to show that Unc13A clusters closer to the active zone centre than Unc13B. Unc13A specifically supported phasic transmission and short-term plasticity of Kenyon cell:output neuron synapses, measured by combining electrophysiological recordings of output neurons with optogenetic stimulation. Knockdown of unc13A within Kenyon cells provoked drastic deficits of olfactory aversive short-term and anaesthesia-sensitive middle-term memory. Knockdown of unc13B provoked milder memory deficits. Thus, a low frequency domain transmission component is probably crucial for the proper representation of memory-associated activity patterns, consistent with sparse Kenyon cell activation during memory acquisition and retrieval. Notably, Unc13A/B ratios appeared highly diversified across MB lobes, leaving room for an interplay of activity components in memory encoding and retrieval."],["dc.identifier.doi","10.1080/01677063.2019.1710146"],["dc.identifier.pmid","31980003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113047"],["dc.identifier.url","https://for2705.de/literature/publications/17"],["dc.language.iso","en"],["dc.relation","FOR 2705: Dissection of a Brain Circuit: Structure, Plasticity and Behavioral Function of the Drosophila Mushroom Body"],["dc.relation","FOR 2705 | TP 5: Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies"],["dc.relation.eissn","1563-5260"],["dc.relation.issn","0167-7063"],["dc.relation.workinggroup","RG Sigrist (Genetics)"],["dc.title","The Unc13A isoform is important for phasic release and olfactory memory formation at mushroom body synapses"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]