Komorowski, Karlo

[["dc.bibliographiccitation.firstpage","43"],["dc.bibliographiccitation.lastpage","86"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Komorowski, Karlo"],["dc.contributor.author","Frank, Kilian"],["dc.contributor.editor","Nieh, Mu-Ping"],["dc.contributor.editor","Heberle, Frederick A."],["dc.contributor.editor","Katsaras, John"],["dc.date.accessioned","2020-03-03T08:14:53Z"],["dc.date.available","2020-03-03T08:14:53Z"],["dc.date.issued","2019"],["dc.description.abstract","In this chapter, we describe X-ray diffraction analysis of lipid model membranes, including fundamentals of experiment and analysis. We start with solid-supported single bilayers and monolayers, then discuss solid-supported multilamellar stacks, and finally vesicles in solution. For oriented membranes, we discuss specular and nonspecular reflectivity, as well as grazing incidence diffraction, and for vesicles we discuss small-angle X-ray scattering. In each case, illustrative examples of current applications are given. The chapter closes with an outlook on free electron laser sources and new opportunities for research on lipid model membranes and biomembranes."],["dc.identifier.doi","10.1515/9783110544657-002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63066"],["dc.language.iso","en"],["dc.publisher","De Gruyter"],["dc.publisher.place","Berlin, Boston"],["dc.relation.eisbn","978-3-11-054465-7"],["dc.relation.ispartof","Characterization of Biological Membranes: Structure and Dynamics"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.title","X-ray structure analysis of lipid membrane systems: solid-supported bilayers, bilayer stacks, and vesicles"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","566"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Biomembranes"],["dc.bibliographiccitation.lastpage","578"],["dc.bibliographiccitation.volume","1860"],["dc.contributor.author","Xu, Yihui"],["dc.contributor.author","Kuhlmann, Jan"],["dc.contributor.author","Brennich, Martha"],["dc.contributor.author","Komorowski, Karlo"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-01-17T13:00:13Z"],["dc.date.available","2018-01-17T13:00:13Z"],["dc.date.issued","2018"],["dc.description.abstract","SNAREs are known as an important family of proteins mediating vesicle fusion. For various biophysical studies, they have been reconstituted into supported single bilayers via proteoliposome adsorption and rupture. In this study we extended this method to the reconstitution of SNAREs into supported multilamellar lipid membranes, i.e. oriented multibilayer stacks, as an ideal model system for X-ray structure analysis (X-ray reflectivity and diffraction). The reconstitution was implemented through a pathway of proteomicelle, proteoliposome and multibilayer. To monitor the structural evolution in each step, we used small-angle X-ray scattering for the proteomicelles and proteoliposomes, followed by X-ray reflectivity and grazing-incidence small-angle scattering for the multibilayers. Results show that SNAREs can be successfully reconstituted into supported multibilayers, with high enough orientational alignment for the application of surface sensitive X-ray characterizations. Based on this protocol, we then investigated the effect of SNAREs on the structure and phase diagram of the lipid membranes. Beyond this application, this reconstitution protocol could also be useful for X-ray analysis of many further membrane proteins."],["dc.identifier.doi","10.1016/j.bbamem.2017.10.023"],["dc.identifier.pmid","29106973"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11697"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.title","Reconstitution of SNARE proteins into solid-supported lipid bilayer stacks and X-ray structure analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]