Bosk, Sabine

[["dc.bibliographiccitation.firstpage","47"],["dc.bibliographiccitation.issue","1-3"],["dc.bibliographiccitation.journal","Biophysical Chemistry"],["dc.bibliographiccitation.lastpage","53"],["dc.bibliographiccitation.volume","150"],["dc.contributor.author","Behn, Daniela"],["dc.contributor.author","Bosk, Sabine"],["dc.contributor.author","Hoffmeister, Helen"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Witzgall, Ralph"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:45:19Z"],["dc.date.available","2017-09-07T11:45:19Z"],["dc.date.issued","2010"],["dc.description.abstract","The pkd1 and pkd2 genes encode for the proteins polycystin-1 (PC1) and polycystin-2 (PC2). These genes are mutated in patients diagnosed with autosomal dominant polycystic kidney disease. PC1 and PC2 interact via their C-terminal, cytosolic regions, which is an essential step in the regulation of cell proliferation and differentiation. Here, we developed an assay that allowed us to quantitatively monitor the interaction of the C-terminal region of PC1 (cPC1) with that of PC2 (cPC2) to be able to answer the question of how Ca2+ influences the PC1/PC2 complex formation. By means of the quartz crystal microbalance (QCM) technique, we were able to determine binding affinities and kinetic constants of the cPC1/cPC2 interaction using a model based on the scaled particle theory. The results suggest that cPC2 forms trimers in solution in the absence of Ca2+, which bind in a one step process to cPC1. (C) 2010 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.bpc.2010.02.005"],["dc.identifier.gro","3142877"],["dc.identifier.isi","000279500100006"],["dc.identifier.pmid","20206434"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/329"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1873-4200"],["dc.relation.issn","0301-4622"],["dc.title","Quantifying the interaction of the C-terminal regions of polycystin-2 and polycystin-1 attached to a lipid bilayer by means of QCM"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]