Frauendorf, Holm

[["dc.bibliographiccitation.firstpage","134"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Toxins"],["dc.bibliographiccitation.lastpage","150"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.contributor.author","Krewer, Birgit"],["dc.contributor.author","von Hof, J. Marian"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Schuberth, Ingrid"],["dc.date.accessioned","2018-11-07T11:21:49Z"],["dc.date.available","2018-11-07T11:21:49Z"],["dc.date.issued","2009"],["dc.description.abstract","The natural antibiotics CC-1065 and the duocarmycins are highly cytotoxic compounds which however are not suitable for cancer therapy due to their general toxicity. We have developed glycosidic prodrugs of seco-analogues of these antibiotics for a selective cancer therapy using conjugates of glycohydrolases and tumour-selective monoclonal antibodies for the liberation of the drugs from the prodrugs predominantly at the tumour site. For the determination of structure activity relationships of the different seco-drugs, experiments addressing their interaction with synthetic DNA were performed. Using electrospray mass spectrometry and high performance liquid chromatography, the experiments revealed a correlation of the stability of these drugs with their cytotoxicity in cell culture investigations. Furthermore, it was shown that the drugs bind to AT-rich regions of double-stranded DNA and the more cytotoxic drugs induce DNA fragmentation at room temperature in several of the selected DNA double-strands. Finally, an explanation for the very high cytotoxicity of CC-1065, the duocarmycins and analogous drugs is given."],["dc.identifier.doi","10.3390/toxins1020134"],["dc.identifier.isi","000208434400006"],["dc.identifier.pmid","22069536"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8258"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55867"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mdpi Ag"],["dc.relation.issn","2072-6651"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Determination of the Biological Activity and Structure Activity Relationships of Drugs Based on the Highly Cytotoxic Duocarmycins and CC-1065"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1139"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Analytical and Bioanalytical Chemistry"],["dc.bibliographiccitation.lastpage","1147"],["dc.bibliographiccitation.volume","390"],["dc.contributor.author","Fitzner, Ansgar"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Laatsch, Hartmut"],["dc.contributor.author","Diederichsen, Ulf"],["dc.date.accessioned","2018-11-07T11:18:24Z"],["dc.date.available","2018-11-07T11:18:24Z"],["dc.date.issued","2008"],["dc.description.abstract","Formation and fragmentation of recognition complexes between trioxacarcin A and various DNA sequences were examined by temperature-dependent UV and CD spectroscopy, HPLC analysis, and ESI mass spectrometry with regard to reaction conditions, intermediates, products, mechanism, and sequence specificity. Cleavage of the trioxacarcin-DNA complexes provided the natural product gutingimycin by guanine abstraction. The resulting DNA with an abasic site was further cleaved into a DNA fragment with a furanyl unit at the 3'-end and an oligonucleotide with a phosphorylated 5'-end."],["dc.identifier.doi","10.1007/s00216-007-1737-6"],["dc.identifier.isi","000252918100017"],["dc.identifier.pmid","18210096"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3482"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55029"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Heidelberg"],["dc.relation.issn","1618-2642"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Formation of gutingimycin: analytical investigation of trioxacarcin A-mediated alkylation of dsDNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","437"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Analytical and Bioanalytical Chemistry"],["dc.bibliographiccitation.lastpage","448"],["dc.bibliographiccitation.volume","395"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.contributor.author","Krewer, Birgit"],["dc.contributor.author","Frauendorf, Holm"],["dc.date.accessioned","2018-11-07T11:24:30Z"],["dc.date.available","2018-11-07T11:24:30Z"],["dc.date.issued","2009"],["dc.description.abstract","One of the main problems of anti-cancer therapy is an insufficient differentiation between normal and malignant cells by the known anti-proliferant agents. The antibody-directed enzyme prodrug therapy is a promising approach for a selective treatment of cancer, in which a non-toxic prodrug is enzymatically converted into a highly cytotoxic drug at the surface of malignant cells by a targeted antibody-enzyme conjugate. The transformations and the stability of a very promising novel prodrug and its corresponding cytotoxic derivative were now investigated in detail by high-performance liquid chromatography (HPLC)-mass spectrometry (MS). In order to determine the time-dependent DNA alkylation efficiency and the sequence selectivity of the novel compounds, DNA binding studies using direct electrospray-Fourier transform ion cyclotron resonance-MS (ESI-FTICR-MS) have been performed. These measurements were accompanied by HPLC analyses followed by MS of the separated species to confirm the results of the direct ESI-FTICR-MS measurements. The sites of DNA alkylation could be identified unambiguously by the mass spectrometric fragmentation pattern of the alkylated oligodeoxynucleotides as well as by the results of HPLC followed by MS. A combination of all techniques applied led to a better understanding of the mode of action of the new therapeutics and might be used for an estimation of the cytotoxicity of different prodrugs and drugs since the alkylation efficiency correlates with the bioactivity of the compounds in cell culture investigations."],["dc.identifier.doi","10.1007/s00216-009-2963-x"],["dc.identifier.isi","000269006500020"],["dc.identifier.pmid","19641906"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3483"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56421"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Heidelberg"],["dc.relation.conference","27th International Symposium on Chromatography"],["dc.relation.eventlocation","Munster, GERMANY"],["dc.relation.issn","1618-2642"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Investigation of the transformations of a novel anti-cancer agent combining HPLC, HPLC-MS and direct ESI-HRMS analyses"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0223552"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Licha, Tobias"],["dc.contributor.author","Jackson, Daniel J."],["dc.contributor.author","Wolkenstein, Klaus"],["dc.date.accessioned","2020-08-04T08:32:28Z"],["dc.date.available","2020-08-04T08:32:28Z"],["dc.date.issued","2019"],["dc.description.abstract","Eumelanin and pheomelanin are well known and common pigments found in nature. However, their complex polymer structure and high thermostability complicate their direct chemical identification. A widely used analytical method is indirect determination using HPLC with UV detection of both types of melanin by their most abundant oxidation products: pyrrole-2,3-dicarboxylic acid (PDCA), pyrrole-2,3,5-tricarboxylic acid (PTCA), thiazole-4,5-dicarboxylic acid (TDCA), and thiazole-2,4,5-tricarboxylic acid (TTCA). An increasing interest in pigmentation in biological research led us to develop a highly sensitive and selective method to identify and quantify these melanin markers in diverse biological samples with complex matrices. By introducing solid-phase extraction (SPE, reversed-phase) following alkaline oxidation we could significantly decrease background signals while maintaining recoveries greater than 70%. Our HPLC-UV-MS method allows for confident peak identification via exact mass information in corresponding UV signals used for quantitation. In addition to synthetic melanin and Sepia officinalis ink as reference compounds eumelanin markers were detected in brown human hair and a brown bivalve shell (Mytilus edulis). Brown feathers from the common chicken (Gallus g. domesticus) yielded all four eumelanin and pheomelanin markers. The present method can be easily adapted for a wide range of future studies on biological samples with unknown melanin content."],["dc.identifier.doi","10.1371/journal.pone.0223552"],["dc.identifier.pmid","31622353"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67513"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Abteilung Geobiologie"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Quantitation of eumelanin and pheomelanin markers in diverse biological samples by HPLC-UV-MS following solid-phase extraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","457"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemistryOpen"],["dc.bibliographiccitation.lastpage","462"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Zlatopolskiy, Boris D."],["dc.contributor.author","Zischler, Johannes"],["dc.contributor.author","Urusova, Elizaveta A."],["dc.contributor.author","Endepols, Heike"],["dc.contributor.author","Kordys, Elena"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Mottaghy, Felix M."],["dc.contributor.author","Neumaier, Bernd"],["dc.date.accessioned","2018-11-07T09:53:27Z"],["dc.date.available","2018-11-07T09:53:27Z"],["dc.date.issued","2015"],["dc.description.abstract","Recently a novel method for the preparation of F-18-labeled arenes via oxidative [F-18] fluorination of easily accessible and sufficiently stable nickel complexes with [F-18] fluoride under exceptionally mild reaction conditions was published. The suitability of this procedure for the routine preparation of clinically relevant positron emission tomography (PET) tracers, 6[-F-18]fluorodopamine (6-[F-18]FDA), 6-[F-18]fluoro-l-DOPA (6-[F-18]FDOPA) and 6-[F-18]fluoro-m-tyrosine (6-[F-18]FMT), was evaluated. The originally published base-free method was inoperative. However, a \"low base\" protocol afforded protected radiolabeled intermediates in radiochemical conversions (RCCs) of 5-18 %. The subsequent deprotection step proceeded almost quantitatively (> 95 %). The simple one-pot two-step procedure allowed the preparation of clinical doses of 6-[F-18]FDA and 6-[F-18]FDOPA within 50 min (12 and 7% radiochemical yield, respectively). In an unilateral rat model of Parkinson's disease, 6-[F-18]FDOPA with high specific activity (175 GBq mu mol(-1)) prepared using the described nickel-mediated radiofluorination was compared to 6-[F-18] FDOPA with low specific activity (30 MBq mu mol(-1)) produced via conventional electrophilic radiofluorination. Unexpectedly both tracer variants displayed very similar in vivo properties with respect to signal-to-noise ratio and brain distribution, and consequently, the quality of the obtained PET images was almost identical."],["dc.identifier.doi","10.1002/open.201500056"],["dc.identifier.isi","000362191700007"],["dc.identifier.pmid","26478840"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12277"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36333"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","2191-1363"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","A Practical One-Pot Synthesis of Positron Emission Tomography (PET) Tracers via Nickel-Mediated Radiofluorination"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6287"],["dc.bibliographiccitation.issue","31"],["dc.bibliographiccitation.journal","Organic & Biomolecular Chemistry"],["dc.bibliographiccitation.lastpage","6293"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Fabritz, Sebastian"],["dc.contributor.author","Hörner, Sebastian"],["dc.contributor.author","Koenning, Doreen"],["dc.contributor.author","Empting, Martin"],["dc.contributor.author","Reinwarth, Michael"],["dc.contributor.author","Dietz, Christian"],["dc.contributor.author","Glotzbach, Bernhard"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Kolmar, Harald"],["dc.contributor.author","Avrutina, Olga"],["dc.date.accessioned","2018-11-07T09:15:10Z"],["dc.date.available","2018-11-07T09:15:10Z"],["dc.date.issued","2012"],["dc.description.abstract","Polyhedral silsesquioxanes are considered valuable conjugation scaffolds. Nevertheless, only a few examples of silsesquioxane-assembled peptide oligomers have been reported to date. We developed a new bioorthogonal cube-octameric silsesquioxane (COSS) scaffold bearing eight aminooxy coupling sites allowing for the conjugation of diverse peptides via oxime ligation. We found that the coupling efficacy depends on the ligand in view of steric hindrance and electrostatic repulsion. For the first time scaffold-based conjugation of cystine-knot miniproteins having a backbone of about thirty amino acids was successfully accomplished without loss of bioactivity. Atomic force microscopy (AFM) provided further knowledge on the size of COSS verifying them as picoscaffolds growing upon bioconjugation to nano-dimension."],["dc.identifier.doi","10.1039/c2ob25728a"],["dc.identifier.isi","000306480100007"],["dc.identifier.pmid","22733169"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10201"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27612"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1477-0520"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7641"],["dc.bibliographiccitation.issue","69"],["dc.bibliographiccitation.journal","Chemical Communications"],["dc.bibliographiccitation.lastpage","7643"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Kenla, Timothee J. Nwemeguela"],["dc.contributor.author","Tatong, Michel D. Kongue"],["dc.contributor.author","Talontsi, Ferdinand Mouafo"],["dc.contributor.author","Dittrich, Birger"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Laatsch, Hartmut"],["dc.date.accessioned","2018-11-07T09:29:42Z"],["dc.date.available","2018-11-07T09:29:42Z"],["dc.date.issued","2013"],["dc.description.abstract","Si-enterobactin (2a), a hexacoordinated complex of the siderophore enterobactin (2b) with silicon as the central atom, was isolated from an endophytic Streptomyces sp. occurring in Piper guinensis roots. The structure and absolute configuration were determined from NMR and MS data, and by X-ray diffraction. The orientation of the molecule along the pseudo-3-fold axis shows that the coordination environment of the silicon atom complexed with three bidentate ligands is Delta. We assume that 2a or related complexes may be involved in the transport of silicon in plants, diatoms, or other silicon-dependent organisms."],["dc.identifier.doi","10.1039/c3cc44437f"],["dc.identifier.isi","000322594900022"],["dc.identifier.pmid","23872808"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10196"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31108"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1364-548X"],["dc.relation.issn","1359-7345"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Si-enterobactin from the endophytic Streptomyces sp KT-S1-B5-a potential silicon transporter in Nature?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3383"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Polymers"],["dc.bibliographiccitation.volume","14"],["dc.contributor.affiliation","Karthäuser, Johannes; 1Department of Wood Biology and Wood Products, Georg-August University of Goettingen, Buesgenweg 4, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Biziks, Vladimirs; 2Surfactor Germany GmbH, Braunschweiger Str. 23 b, 38170 Schoeppenstedt, Germany"],["dc.contributor.affiliation","Frauendorf, Holm; 3Institute of Organic and Biomolecular Chemistry, Georg-August University of Goettingen, Tammannstraße 2, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Mai, Carsten; 1Department of Wood Biology and Wood Products, Georg-August University of Goettingen, Buesgenweg 4, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Militz, Holger; 1Department of Wood Biology and Wood Products, Georg-August University of Goettingen, Buesgenweg 4, 37077 Goettingen, Germany"],["dc.contributor.author","Karthäuser, Johannes"],["dc.contributor.author","Biziks, Vladimirs"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Mai, Carsten"],["dc.contributor.author","Militz, Holger"],["dc.contributor.editor","Alves, Luis"],["dc.date.accessioned","2022-09-01T09:51:17Z"],["dc.date.available","2022-09-01T09:51:17Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:14:51Z"],["dc.description.abstract","Cleavage by microwave-assisted pyrolysis is a way to obtain higher-value organic chemicals from technical lignins. In this report, pine kraft lignin (PKL), spruce and beech organosolv lignin (SOSL and BOSL), and calcium lignosulfonates from spruce wood (LS) were pyrolyzed at temperatures between 30 and 280 °C using vacuum low-temperature, microwave-assisted pyrolysis. The mass balance, energy consumption, condensation rate, and pressure changes of the products during the pyrolysis process were recorded. Phenolic condensates obtained at different temperatures during pyrolysis were collected, and their chemical composition was determined by GC-MS and GC-FID. The origin of the technical lignin had a significant influence on the pyrolysis products. Phenolic condensates were obtained in yields of approximately 15% (PKL and SOSL) as well as in lower yields of 4.5% (BOSL) or even 1.7% (LS). The main production of the phenolic condensates for the PKL and SOSL occurred at temperatures of approximately 140 and 180 °C, respectively. The main components of the phenolic fraction of the three softwood lignins were guaiacol, 4-methylguaiacol, 4-ethylguaiacol, and other guaiacol derivatives; however, the quantity varied significantly depending on the lignin source. Due to the low cleavage temperature vacuum, low-temperature, microwave-assisted pyrolysis could be an interesting approach to lignin conversion."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3390/polym14163383"],["dc.identifier.pii","polym14163383"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113923"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4360"],["dc.rights","Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."],["dc.title","Vacuum Low-Temperature Microwave-Assisted Pyrolysis of Technical Lignins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Bioengineering and Biotechnology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Gerke, Jennifer"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Wintergoller, Maxim"],["dc.contributor.author","Hofmeister, Thomas"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Zebec, Ziga"],["dc.contributor.author","Takano, Eriko"],["dc.contributor.author","Scrutton, Nigel S."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2021-04-14T08:32:40Z"],["dc.date.available","2021-04-14T08:32:40Z"],["dc.date.issued","2020"],["dc.description.abstract","Monoterpenoids, such as the plant metabolite geraniol, are of high industrial relevance since they are important fragrance materials for perfumes, cosmetics, and household products. Chemical synthesis or extraction from plant material for industry purposes are complex, environmentally harmful or expensive and depend on seasonal variations. Heterologous microbial production offers a cost-efficient and sustainable alternative but suffers from low metabolic flux of the precursors and toxicity of the monoterpenoid to the cells. In this study, we evaluated two approaches to counteract both issues by compartmentalizing the biosynthetic enzymes for geraniol to the peroxisomes of Saccharomyces cerevisiae as production sites and by improving the geraniol tolerance of the yeast cells. The combination of both approaches led to an 80% increase in the geraniol titers. In the future, the inclusion of product tolerance and peroxisomal compartmentalization into the general chassis engineering toolbox for monoterpenoids or other host-damaging, industrially relevant metabolites may lead to an efficient, low-cost, and eco-friendly microbial production for industrial purposes."],["dc.identifier.doi","10.3389/fbioe.2020.582052"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83978"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","2296-4185"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Production of the Fragrance Geraniol in Peroxisomes of a Product-Tolerant Baker’s Yeast"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1005205"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Lin, Chi-Jan"],["dc.contributor.author","Sasse, Christoph"],["dc.contributor.author","Gerke, Jennifer"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Irmer, Henriette"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Heinekamp, Thorsten"],["dc.contributor.author","Strassburger, Maria"],["dc.contributor.author","van Tuan Tran, Van Tuan Tran"],["dc.contributor.author","Herzog, Britta"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T09:49:12Z"],["dc.date.available","2018-11-07T09:49:12Z"],["dc.date.issued","2015"],["dc.description.abstract","The transcription factor Flo8/Som1 controls filamentous growth in Saccharomyces cerevisiae and virulence in the plant pathogen Magnaporthe oryzae. Flo8/Som1 includes a characteristic N-terminal LUG/LUH-Flo8-single-stranded DNA binding (LUFS) domain and is activated by the cAMP dependent protein kinase A signaling pathway. Heterologous SomA from Aspergillus fumigatus rescued in yeast flo8 mutant strains several phenotypes including adhesion or flocculation in haploids and pseudohyphal growth in diploids, respectively. A. fumigatus SomA acts similarly to yeast Flo8 on the promoter of FLO11 fused with reporter gene (LacZ) in S. cerevisiae. FLO11 expression in yeast requires an activator complex including Flo8 and Mfg1. Furthermore, SomA physically interacts with PtaB, which is related to yeast Mfg1. Loss of the somA gene in A. fumigatus resulted in a slow growth phenotype and a block in asexual development. Only aerial hyphae without further differentiation could be formed. The deletion phenotype was verified by a conditional expression of somA using the inducible Tet-on system. A adherence assay with the conditional somA expression strain indicated that SomA is required for biofilm formation. A ptaB deletion strain showed a similar phenotype supporting that the SomA/PtaB complex controls A. fumigatus biofilm formation. Transcriptional analysis showed that SomA regulates expression of genes for several transcription factors which control conidiation or adhesion of A. fumigatus. Infection assays with fertilized chicken eggs as well as with mice revealed that SomA is required for pathogenicity. These data corroborate a complex control function of SomA acting as a central factor of the transcriptional network, which connects adhesion, spore formation and virulence in the opportunistic human pathogen A. fumigatus."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.ppat.1005205"],["dc.identifier.isi","000368332000007"],["dc.identifier.pmid","26529322"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12564"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35459"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7374"],["dc.relation.issn","1553-7366"],["dc.rights.access","openAccess"],["dc.title","Transcription Factor SomA Is Required for Adhesion, Development and Virulence of the Human Pathogen Aspergillus fumigatus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]