Guan, Kaomei

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Stem Cell Research"],["dc.bibliographiccitation.lastpage","154"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Vlasov, Alla"],["dc.contributor.author","Huelsmann, Swen"],["dc.contributor.author","Yin, Dongjiao"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2017-09-07T11:47:33Z"],["dc.date.available","2017-09-07T11:47:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Recently, we reported the successful establishment of multipotent adult germ-Line stem cells (maGSCs) from cultured adult mouse spermatogonial stem cells. Similar to embryonic stem cells, maGSCs are able to self-renew and differentiate into derivatives of all three germ Layers. These properties make maGSCs a potential cell source for the treatment of neural degenerative diseases. In this study, we describe the generation of maGSC-derived proliferating neural precursor cells using growth factor-mediated neural Lineage induction. The neural precursors were positive for nestin and Sox1 and could be continuously expanded. Upon further differentiation, they formed functional neurons and glial cells, as demonstrated by expression of lineage-restricted genes and proteins and by electrophysiological properties. Characterization of maGSC-derived neurons revealed the generation of specific subtypes, including GABAergic, glutamatergic, serotonergic, and dopaminergic neurons. Electrophysiological analysis revealed passive and active membrane properties and postsynaptic currents, indicating their functional maturation. Functional networks formed at later stages of differentiation, as evidenced by synaptic transmission of spontaneous neuronal activity. In conclusion, our data demonstrate that maGSCs may be used as a new stem cell source for basic research and biomedical. applications. (C) 2008 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.scr.2008.09.001"],["dc.identifier.gro","3143148"],["dc.identifier.isi","000272224500006"],["dc.identifier.pmid","19383419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/630"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1873-5061"],["dc.title","Generation of functional neurons and glia from multipotent adult mouse germ-line stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Blood"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Schreiter, Jessica"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Nayemia, Karim"],["dc.contributor.author","Chapuy, Bjoem"],["dc.contributor.author","Truemper, Lorenz H."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Wulf, Gerald G."],["dc.date.accessioned","2018-11-07T08:57:49Z"],["dc.date.available","2018-11-07T08:57:49Z"],["dc.date.issued","2006"],["dc.format.extent","480A"],["dc.identifier.isi","000242440002204"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23491"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Hematology"],["dc.publisher.place","Washington"],["dc.relation.eventlocation","Orlando, FL"],["dc.relation.issn","0006-4971"],["dc.title","Hematopoietic and endothelial progenitor cells from mouse adult testis derived stem cell lines."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Tissue Antigens"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Monecke, Sebastian"],["dc.contributor.author","Hamann, Carina"],["dc.contributor.author","Elsner, Leslie"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Mansouri, Ahmed"],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T09:38:32Z"],["dc.date.available","2018-11-07T09:38:32Z"],["dc.date.issued","2014"],["dc.format.extent","5"],["dc.identifier.isi","000337546000002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33083"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.eventlocation","Stockholm, SWEDEN"],["dc.relation.issn","1399-0039"],["dc.relation.issn","0001-2815"],["dc.title","PLURIPOTENT STEM CELLS VARYING IN A SINGLE MINOR HISTOCOMPATIBILITY ANTIGEN ELICIT CELLULAR AND HUMORAL IMMUNE RESPONSES THAT CAN MEDIATE GRAFT REJECTION"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1199"],["dc.bibliographiccitation.issue","7088"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","1203"],["dc.bibliographiccitation.volume","440"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Wolf, F"],["dc.contributor.author","Li, M. Y."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2017-09-07T11:53:07Z"],["dc.date.available","2017-09-07T11:53:07Z"],["dc.date.issued","2006"],["dc.description.abstract","Embryonic germ cells as well as germline stem cells from neonatal mouse testis are pluripotent and have differentiation potential similar to embryonic stem cells(1,2), suggesting that the germline lineage may retain the ability to generate pluripotent cells. However, until now there has been no evidence for the pluripotency and plasticity of adult spermatogonial stem cells (SSCs), which are responsible for maintaining spermatogenesis throughout life in the male(3). Here we show the isolation of SSCs from adult mouse testis using genetic selection, with a success rate of 27%. These isolated SSCs respond to culture conditions and acquire embryonic stem cell properties. We name these cells multipotent adult germline stem cells (maGSCs). They are able to spontaneously differentiate into derivatives of the three embryonic germ layers in vitro and generate teratomas in immunodeficient mice. When injected into an early blastocyst, SSCs contribute to the development of various organs and show germline transmission. Thus, the capacity to form multipotent cells persists in adult mouse testis. Establishment of human maGSCs from testicular biopsies may allow individual cell-based therapy without the ethical and immunological problems associated with human embryonic stem cells. Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages."],["dc.identifier.doi","10.1038/nature04697"],["dc.identifier.gro","3143702"],["dc.identifier.isi","000237080000047"],["dc.identifier.pmid","16565704"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1245"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Pluripotency of spermatogonial stem cells from adult mouse testis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","521"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Molecular Human Reproduction"],["dc.bibliographiccitation.lastpage","529"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Zovoilis, Athanasios"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Drusenheimer, Nadja"],["dc.contributor.author","Zechner, Ulrich"],["dc.contributor.author","Hada, Hiroki"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2017-09-07T11:48:13Z"],["dc.date.available","2017-09-07T11:48:13Z"],["dc.date.issued","2008"],["dc.description.abstract","Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues."],["dc.identifier.doi","10.1093/molehr/gan044"],["dc.identifier.gro","3143248"],["dc.identifier.isi","000259585200003"],["dc.identifier.pmid","18697907"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/741"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Research Foundation [SPP 1356, EN 84/22-1, ZE 442/4-1]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1360-9947"],["dc.title","Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.volume","97"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Wagner, S."],["dc.contributor.author","Wolf, F."],["dc.contributor.author","Li, M. Y."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd P."],["dc.date.accessioned","2018-11-07T10:54:06Z"],["dc.date.available","2018-11-07T10:54:06Z"],["dc.date.issued","2005"],["dc.format.extent","1200"],["dc.identifier.isi","000233460900020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49496"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","Scientific Meeting of the American-Heart-Association"],["dc.relation.eventlocation","Dallas, TX"],["dc.relation.issn","0009-7330"],["dc.title","Adult mouse spermatogonial stem cells differentiate into cardiovascular lineages and generate functional cardiomyocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","26"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.lastpage","29"],["dc.bibliographiccitation.volume","213"],["dc.contributor.author","Mardanpour, Parisa"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nayernia, Karim"],["dc.date.accessioned","2017-09-07T11:48:16Z"],["dc.date.available","2017-09-07T11:48:16Z"],["dc.date.issued","2008"],["dc.description.abstract","Germline stem cells, which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. The germ cells have a special place in the life cycle because they must be able to retain the ability to recreate the organism, a property known as developmental totipotency. Several lines of evidence have suggested the extensive proliferation activity and pluripotency of prenatal, neonatal and adult germline stem cells. We showed that adult male germline stem cells, spermatogonial stem cells, can be converted into embryonic stem cell-like cells, which can differentiate into the somatic stem cells of three germ layers. Different cell types such as vascular, heart, liver, pancreatic and blood cells could also be obtained from these stem cells. Understanding how spermatogonial stem cells can give rise to pluripotent stem cells and how somatic stem cells differentiate into germ cells could give significant insight into the regulation of developmental totipotency as well as having important implications for male fertility and regenerative medicine."],["dc.identifier.doi","10.1111/j.1469-7580.2008.00930.x"],["dc.identifier.gro","3143270"],["dc.identifier.isi","000257707900003"],["dc.identifier.pmid","18565110"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/765"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.conference","International Conference on Advances in Stem Cell Biology"],["dc.relation.eventlocation","Durham Univ, Durham, ENGLAND"],["dc.relation.ispartof","Journal of Anatomy"],["dc.relation.issn","0021-8782"],["dc.title","Potency of germ cells and its relevance for regenerative medicine"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","41"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE"],["dc.bibliographiccitation.lastpage","44"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Drusenheimer, Nadja"],["dc.contributor.author","Rathsack, Kristina"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Ehrmann, Ingrid E."],["dc.contributor.author","Elliott, David J."],["dc.contributor.author","Zechner, Ulrich"],["dc.contributor.author","Haaf, Thomas"],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Michelmann, Hans Wilhelm"],["dc.contributor.author","Hasenfuss, Gerlad"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2018-11-07T11:04:31Z"],["dc.date.available","2018-11-07T11:04:31Z"],["dc.date.issued","2007"],["dc.description.abstract","Germline and somatic stem cells are distinct types of stem cells that are dedicated to reproduction and somatic tissue regeneration, respectively. Germline stem cells (GSCs), which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. We developed a strategy for the establishment of germline stem cell lines from embryonic stem cells (ES). These cells are able to undergo meiosis, generate haploid male gametes in vitro and are functional, as shown by fertilization after intra-cytoplasmic injection into mouse oocytes. In other approach, we show that bone marrow stem (BMS) cells are able to trans-differentiate into male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells. The ability to derive male germ cells from ES and BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine. Conversely, we showed that adult male germline stem cells, spermatogonial stem cells (SSCs), can be converted into embryonic stem cell like cells which can differentiate into the somatic stem cells of three germ layers. Understanding how SSC can give rise to pluripotent stem cells and how somatic stem cells differentiate into germ cells could give significant insights into the regulation of developmental totipotency as well as having important implications for male fertility and regenerative medicine."],["dc.identifier.isi","000254385400001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51863"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1680-6433"],["dc.title","From stem cells to germ cells and from germ cells to stem cells"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","394"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Tissue Antigens"],["dc.bibliographiccitation.lastpage","395"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Elsner, Leslie"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T08:30:35Z"],["dc.date.available","2018-11-07T08:30:35Z"],["dc.date.issued","2009"],["dc.identifier.isi","000266032200026"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16928"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.publisher.place","Malden"],["dc.relation.eventlocation","Ulm, GERMANY"],["dc.relation.issn","0001-2815"],["dc.title","Multipotent adult germline stem cells and embryonic stem cells are targets for cytotoxic T lymphocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1615"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1625"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Kaiser, Diana"],["dc.contributor.author","Hemmerlein, Bernhard"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2017-09-07T11:49:27Z"],["dc.date.available","2017-09-07T11:49:27Z"],["dc.date.issued","2007"],["dc.description.abstract","Stem cell-based therapy is a promising approach for the treatment of heart failure. Adult stem cells with the pluripotency of embryonic stem cells (ESCs) would be an ideal cell source. Recently, we reported the successful establishment of multipotent adult germline stem cells (maGSCs) from mouse testis. These cultured maGSCs show phenotypic characteristics similar to ESCs and can spontaneously differentiate into cells from all 3 germ layers. In the present study, we used the hanging drop method to differentiate maGSCs into cardiomyocytes and analyzed their functional properties. Differentiation efficiency of beating cardiomyocytes from maGSCs was similar to that from ESCs. The maGSC-derived cardiomyocytes expressed cardiac-specific L-type Ca2+ channels and responded to Ca2+ channel-modulating drugs. Cx43 was expressed at cell-to-cell contacts in cardiac clusters, and fluorescence recovery after photobleaching assay showed the presence of functional gap junctions among cardiomyocytes. Action potential analyses demonstrated the presence of pacemaker-, ventricle-, atrial-, and Purkinje-like cardiomyocytes. Stimulation with isoproterenol resulted in a significant increase in beating frequency, whereas the addition of cadmium chloride abolished spontaneous electrical activity. Confocal microscopy analysis of intracellular Ca2+ in maGSC-derived cardiomyocytes showed that calcium increased periodically throughout the cell in a homogenous fashion, pointing to a fine regulated Ca2+ release from intracellular Ca2+ stores. By using line-scan mode, we found rhythmic Ca2+ transients. Furthermore, we transplanted maGSCs into normal hearts of mice and found that maGSCs were able to proliferate and differentiate. No tumor formation was found up to 1 month after cell transplantation. Taken together, we believe that maGSCs provide a new source of distinct types of cardiomyocytes for basic research and potential therapeutic application."],["dc.identifier.doi","10.1161/01.RES.0000269182.22798.d9"],["dc.identifier.gro","3143484"],["dc.identifier.isi","000247077300013"],["dc.identifier.pmid","17478732"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1003"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0009-7330"],["dc.title","Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]