Guan, Kaomei

[["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schmidt, A."],["dc.contributor.author","Koegler, Harald"],["dc.contributor.author","Guan-Schmidt, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T10:58:53Z"],["dc.date.available","2018-11-07T10:58:53Z"],["dc.date.issued","2007"],["dc.format.extent","8"],["dc.identifier.isi","000208702200022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50568"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.issn","0195-668X"],["dc.title","Disturbed MAPK signaling and heart failure in Impedes Mitogenic Signaling (IMP) transgenic mice"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.volume","105"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Maenner, Joerg"],["dc.contributor.author","Schmidt, Albrecht"],["dc.contributor.author","Kurz, Kathrin"],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Kramann, Nadine"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:24:12Z"],["dc.date.available","2018-11-07T11:24:12Z"],["dc.date.issued","2009"],["dc.format.extent","E15"],["dc.identifier.isi","000270150800039"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56352"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","Basic Cardiovascular Sciences Conference 2009"],["dc.relation.eventlocation","Lake Las Vegas, Henderson, NV"],["dc.relation.issn","0009-7330"],["dc.title","Impedes Mitogenic Signal Propagation (IMP) Is Essential for Embryonic Heart and Brain Development and Controls Cardiac Performance via MAPK Signaling in Vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Boecker, C."],["dc.contributor.author","Schmidt, A."],["dc.contributor.author","Kurz, K."],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:24:44Z"],["dc.date.available","2018-11-07T11:24:44Z"],["dc.date.issued","2009"],["dc.format.extent","219"],["dc.identifier.isi","000208702602277"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56474"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.issn","0195-668X"],["dc.title","Impedes Mitogenic Signal Propagation (IMP) is responsive towards neurohumoral stimulation controlling MAPK mediated development and cardiac growth"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schmidt, Albrecht"],["dc.contributor.author","Kurz, Kathrin"],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Kramann, Nadine"],["dc.contributor.author","Guan-Schmidt, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:09:54Z"],["dc.date.available","2018-11-07T11:09:54Z"],["dc.date.issued","2008"],["dc.format.extent","S423"],["dc.identifier.isi","000262104500692"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53102"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","81st Annual Scientific Session of the American-Heart-Association"],["dc.relation.eventlocation","New Orleans, LA"],["dc.relation.issn","0009-7322"],["dc.title","Impedes Mitogenic Signal Propagation (IMP) Is Essential for Embryonic Development and Controls Cardiac Size and Function via MAPK"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","401"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","412"],["dc.bibliographiccitation.volume","117"],["dc.contributor.author","Chang Liao, Mei-Ling"],["dc.contributor.author","Boer, Teun P. de"],["dc.contributor.author","Mutoh, Hiroki"],["dc.contributor.author","Raad, Nour"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Downie, Bryan R."],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Arooj, Iqra"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Doeker, Stephan"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Wettwer, Erich"],["dc.contributor.author","van Veen, Toon"],["dc.contributor.author","Morlock, Michael M."],["dc.contributor.author","Knoepfel, Thomas"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.date.accessioned","2017-09-07T11:43:37Z"],["dc.date.available","2017-09-07T11:43:37Z"],["dc.date.issued","2015"],["dc.description.abstract","Rationale: Monitoring and controlling cardiac myocyte activity with optogenetic tools offer exciting possibilities for fundamental and translational cardiovascular research. Genetically encoded voltage indicators may be particularly attractive for minimal invasive and repeated assessments of cardiac excitation from the cellular to the whole heart level. Objective: To test the hypothesis that cardiac myocyte-targeted voltage-sensitive fluorescence protein 2.3 (VSFP2.3) can be exploited as optogenetic tool for the monitoring of electric activity in isolated cardiac myocytes and the whole heart as well as function and maturity in induced pluripotent stem cell-derived cardiac myocytes. Methods and Results: We first generated mice with cardiac myocyte-restricted expression of VSFP2.3 and demonstrated distinct localization of VSFP2.3 at the t-tubulus/junctional sarcoplasmic reticulum microdomain without any signs for associated pathologies (assessed by echocardiography, RNA-sequencing, and patch clamping). Optically recorded VSFP2.3 signals correlated well with membrane voltage measured simultaneously by patch clamping. The use of VSFP2.3 for human action potential recordings was confirmed by simulation of immature and mature action potentials in murine VSFP2.3 cardiac myocytes. Optical cardiograms could be monitored in whole hearts ex vivo and minimally invasively in vivo via fiber optics at physiological heart rate (10 Hz) and under pacing-induced arrhythmia. Finally, we reprogrammed tail-tip fibroblasts from transgenic mice and used the VSFP2.3 sensor for benchmarking functional and structural maturation in induced pluripotent stem cell-derived cardiac myocytes. Conclusions: We introduce a novel transgenic voltage-sensor model as a new method in cardiovascular research and provide proof of concept for its use in optogenetic sensing of physiological and pathological excitation in mature and immature cardiac myocytes in vitro and in vivo."],["dc.identifier.doi","10.1161/CIRCRESAHA.117.306143"],["dc.identifier.gro","3141844"],["dc.identifier.isi","000359658700005"],["dc.identifier.pmid","26078285"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1701"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/84"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A03: Bedeutung CaMKII-abhängiger Mechanismen für die Arrhythmogenese bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A04: Patienten-spezifische induzierte pluripotente Stammzellen zur funktionellen Untersuchung von Ryanodinrezeptor-Mutationen"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation","SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","0009-7330"],["dc.relation.workinggroup","RG Guan (Application of patient-specific induced pluripotent stem cells in disease modelling)"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.relation.workinggroup","RG L. Maier (Experimentelle Kardiologie)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.title","Sensing Cardiac Electrical Activity With a Cardiac Myocyte-Targeted Optogenetic Voltage Indicator"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schmidt, Albrecht"],["dc.contributor.author","Kurz, Katrin"],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:12:01Z"],["dc.date.available","2018-11-07T11:12:01Z"],["dc.date.issued","2008"],["dc.format.extent","E46"],["dc.identifier.isi","000258845200070"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53564"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","Basic Cardiovascular Sciences Conference"],["dc.relation.eventlocation","Keystone, CO"],["dc.relation.issn","0009-7330"],["dc.title","Impedes Mitogenic Signal Propagation (IMP) Is Essential for Embryonic Development but Mediates Detrimental Cardiac MAPK Signaling In Vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1615"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1625"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Kaiser, Diana"],["dc.contributor.author","Hemmerlein, Bernhard"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2017-09-07T11:49:27Z"],["dc.date.available","2017-09-07T11:49:27Z"],["dc.date.issued","2007"],["dc.description.abstract","Stem cell-based therapy is a promising approach for the treatment of heart failure. Adult stem cells with the pluripotency of embryonic stem cells (ESCs) would be an ideal cell source. Recently, we reported the successful establishment of multipotent adult germline stem cells (maGSCs) from mouse testis. These cultured maGSCs show phenotypic characteristics similar to ESCs and can spontaneously differentiate into cells from all 3 germ layers. In the present study, we used the hanging drop method to differentiate maGSCs into cardiomyocytes and analyzed their functional properties. Differentiation efficiency of beating cardiomyocytes from maGSCs was similar to that from ESCs. The maGSC-derived cardiomyocytes expressed cardiac-specific L-type Ca2+ channels and responded to Ca2+ channel-modulating drugs. Cx43 was expressed at cell-to-cell contacts in cardiac clusters, and fluorescence recovery after photobleaching assay showed the presence of functional gap junctions among cardiomyocytes. Action potential analyses demonstrated the presence of pacemaker-, ventricle-, atrial-, and Purkinje-like cardiomyocytes. Stimulation with isoproterenol resulted in a significant increase in beating frequency, whereas the addition of cadmium chloride abolished spontaneous electrical activity. Confocal microscopy analysis of intracellular Ca2+ in maGSC-derived cardiomyocytes showed that calcium increased periodically throughout the cell in a homogenous fashion, pointing to a fine regulated Ca2+ release from intracellular Ca2+ stores. By using line-scan mode, we found rhythmic Ca2+ transients. Furthermore, we transplanted maGSCs into normal hearts of mice and found that maGSCs were able to proliferate and differentiate. No tumor formation was found up to 1 month after cell transplantation. Taken together, we believe that maGSCs provide a new source of distinct types of cardiomyocytes for basic research and potential therapeutic application."],["dc.identifier.doi","10.1161/01.RES.0000269182.22798.d9"],["dc.identifier.gro","3143484"],["dc.identifier.isi","000247077300013"],["dc.identifier.pmid","17478732"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1003"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0009-7330"],["dc.title","Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Schott, Peter"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schmidt, A."],["dc.contributor.author","Kurz, K."],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Guan-Schmidt, Kaomei"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:11:20Z"],["dc.date.available","2018-11-07T11:11:20Z"],["dc.date.issued","2008"],["dc.format.extent","898"],["dc.identifier.isi","000208702504438"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53408"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.issn","0195-668X"],["dc.title","Impedes Mitogenic signal Propagation (IMP) is essential for embryonic development but mediates detrimental cardiac MAPK signalling in vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","430"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Heart Failure"],["dc.bibliographiccitation.lastpage","437"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schotola, Hanna"],["dc.contributor.author","Jacobshagen, Claudius"],["dc.contributor.author","Seidler, Tim"],["dc.contributor.author","Sossalla, Samuel"],["dc.contributor.author","Emmons, Julius"],["dc.contributor.author","Klede, Stefanie"],["dc.contributor.author","Knoell, Ralph"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","El-Armouche, Ali"],["dc.contributor.author","Linke, Wolfgang A."],["dc.contributor.author","Koegler, Harald"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2017-09-07T11:48:55Z"],["dc.date.available","2017-09-07T11:48:55Z"],["dc.date.issued","2012"],["dc.description.abstract","Muscle LIM protein (MLP) null mice are often used as a model for human dilated cardiomyopathy. So far, little is known about the time course and pathomechanisms leading to the development of the adult phenotype. We systematically analysed the contractile phenotype, myofilament calcium (Ca-2) responsiveness, passive myocardial mechanics, histology, and mRNA expression in mice aged 4 and 12 weeks. In 4-week-old animals, there was no significant difference in the forcefrequency relationship (FFR) and catecholamine response of intact isolated papillary muscles between wild-type (WT) and MLP null myocardium. In 12-week-old animals, WT myocardium exhibited a significantly positive FFR, while that of MLP null mice was significantly negative, and the inotropic response to catecholamines was significantly reduced in MLP null mice. This time course of decline in contractile function was confirmed in vivo by echocardiography. Whereas at 4 weeks of age MLP null mice and WT littermates showed similar levels of SERCA2a (sarcoplasmic reticulum Ca-2 ATPase) expression, the expression was significantly lower in 12-week-old MLP null mice compared with littermate controls. Myofilament Ca-2 responsiveness was not affected by the lack of MLP, irrespective of age. Whereas in 4-week-old animals MLP null myocardium showed a trend to an increased compliance compared with the WT, myocardium of 12-week-old MLP null mice was significantly less compliant than WT myocardium. Parallel to the decrease in compliance there was an increase in fibrosis in the MLP null animals. Our data suggest that MLP deficiency does not primarily influence myocardial contractility. A lack of MLP leads to an age-dependent impairment of excitationcontraction coupling with resulting contractile dysfunction and secondary fibrosis."],["dc.identifier.doi","10.1093/eurjhf/hfs020"],["dc.identifier.gro","3142557"],["dc.identifier.isi","000301973500013"],["dc.identifier.pmid","22371524"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8921"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1388-9842"],["dc.title","Age-dependent changes in contractile function and passive elastic properties of myocardium from mice lacking muscle LIM protein (MLP)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Molecular and Cellular Cardiology"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Kaiser, Diana"],["dc.contributor.author","Cheng, I.-F."],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schaefer, K."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:10:39Z"],["dc.date.available","2018-11-07T11:10:39Z"],["dc.date.issued","2008"],["dc.format.extent","S1"],["dc.identifier.doi","10.1016/j.yjmcc.2008.09.592"],["dc.identifier.isi","000260844600003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53253"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Ltd- Elsevier Science Ltd"],["dc.publisher.place","London"],["dc.relation.conference","25th Annual Meeting of the Japanese Section of the International-Society-for-Heart-Research"],["dc.relation.eventlocation","Yokohama, JAPAN"],["dc.relation.issn","1095-8584"],["dc.relation.issn","0022-2828"],["dc.title","Distingished Award Lecture Adult Pluripotent Cells for Cardiac Regeneration"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]