Streit, Frank

[["dc.bibliographiccitation.artnumber","1378056"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Extracellular Vesicles"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Sönmezer, Can"],["dc.contributor.author","Worst, Thomas Stefan"],["dc.contributor.author","Schulz, Matthias"],["dc.contributor.author","Dihazi, Gry Helene"],["dc.contributor.author","Streit, Frank"],["dc.contributor.author","Erdmann, Gerrit"],["dc.contributor.author","Kling, Simon"],["dc.contributor.author","Boutros, Michael"],["dc.contributor.author","Binder, Claudia"],["dc.contributor.author","Gross, Julia Christina"],["dc.date.accessioned","2019-07-09T11:45:57Z"],["dc.date.available","2019-07-09T11:45:57Z"],["dc.date.issued","2017"],["dc.description.abstract","Extracellular vesicles (EVs) are membrane particles secreted from cells into all body fluids. Several EV populations exist differing in size and cellular origin. Using differential centrifugation EVs pelleting at 14,000 g (\"microvesicles\" (MV)) and 100,000 g (\"exosomes\") are distinguishable by protein markers. Neutral sphingomyelinase (nSMase) inhibition has been shown to inhibit exosome release from cells and has since been used to study their functional implications. How nSMases (also known as SMPD2 and SMPD3) affect the basal secretion of MVs is unclear. Here we investigated how SMPD2/3 impact both EV populations. SMPD2/3 inhibition by GW4869 or RNAi decreases secretion of exosomes, but also increases secretion of MVs from the plasma membrane. Both populations differ significantly in metabolite composition and Wnt proteins are specifically loaded onto MVs under these conditions. Taken together, our data reveal a novel regulatory function of SMPD2/3 in vesicle budding from the plasma membrane and clearly suggest that - despite the different vesicle biogenesis - the routes of vesicular export are adaptable."],["dc.identifier.doi","10.1080/20013078.2017.1378056"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15354"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59345"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2001-3078"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1155"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Translational psychiatry"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Witt, S. H."],["dc.contributor.author","Streit, F,"],["dc.contributor.author","Jungkunz, M."],["dc.contributor.author","Frank, J."],["dc.contributor.author","Awasthi, S."],["dc.contributor.author","Reinbold, C. S."],["dc.contributor.author","Treutlein, J."],["dc.contributor.author","Degenhardt, F."],["dc.contributor.author","Forstner, A. J."],["dc.contributor.author","Heilmann-Heimbach, S."],["dc.contributor.author","McQuillin, A."],["dc.date.accessioned","2019-07-09T11:44:56Z"],["dc.date.available","2019-07-09T11:44:56Z"],["dc.date.issued","2017-06-20"],["dc.description.abstract","Borderline personality disorder (BOR) is determined by environmental and genetic factors, and characterized by affective instability and impulsivity, diagnostic symptoms also observed in manic phases of bipolar disorder (BIP). Up to 20% of BIP patients show comorbidity with BOR. This report describes the first case-control genome-wide association study (GWAS) of BOR, performed in one of the largest BOR patient samples worldwide. The focus of our analysis was (i) to detect genes and gene sets involved in BOR and (ii) to investigate the genetic overlap with BIP. As there is considerable genetic overlap between BIP, major depression (MDD) and schizophrenia (SCZ) and a high comorbidity of BOR and MDD, we also analyzed the genetic overlap of BOR with SCZ and MDD. GWAS, gene-based tests and gene-set analyses were performed in 998 BOR patients and 1545 controls. Linkage disequilibrium score regression was used to detect the genetic overlap between BOR and these disorders. Single marker analysis revealed no significant association after correction for multiple testing. Gene-based analysis yielded two significant genes: DPYD (P=4.42 × 10-7) and PKP4 (P=8.67 × 10-7); and gene-set analysis yielded a significant finding for exocytosis (GO:0006887, PFDR=0.019; FDR, false discovery rate). Prior studies have implicated DPYD, PKP4 and exocytosis in BIP and SCZ. The most notable finding of the present study was the genetic overlap of BOR with BIP (rg=0.28 [P=2.99 × 10-3]), SCZ (rg=0.34 [P=4.37 × 10-5]) and MDD (rg=0.57 [P=1.04 × 10-3]). We believe our study is the first to demonstrate that BOR overlaps with BIP, MDD and SCZ on the genetic level. Whether this is confined to transdiagnostic clinical symptoms should be examined in future studies."],["dc.identifier.doi","10.1038/tp.2017.115"],["dc.identifier.pmid","28632202"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14981"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59129"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2158-3188"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Genome-wide association study of borderline personality disorder reveals genetic overlap with bipolar disorder, major depression and schizophrenia."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]