Weig, Michael

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Taverne-Ghadwal, Liliane"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Schulze, Marco H."],["dc.contributor.author","Mbaitolum, Weina Joseph"],["dc.contributor.author","Kersch, Lydia"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Groß, U."],["dc.date.accessioned","2022-04-01T10:00:45Z"],["dc.date.available","2022-04-01T10:00:45Z"],["dc.date.issued","2022"],["dc.description.abstract","Oral candidiasis remains a common problem in HIV-infected individuals, especially in sub-Saharan Africa. Here, we performed the first study in Chad on the prevalence of oral yeasts carriage and oral candidiasis in HIV-positive subjects from southern Chad and analyzed the influence of HAART, CD4 + T-cell numbers, and antimycotics in 589 patients. These patients were recruited from a specialized medical center for HIV patients in Sarh and from a rural medical health dispensary in the vicinity, including a total of 384 HIV-positive and 205 HIV-negative individuals. Yeasts obtained from oral specimen were identified by MALDI-TOF MS and their antifungal susceptibility profiles determined. The overall prevalence of yeast colonization and symptomatic oral candidiasis in HIV-infected patients was 25.1%. The prevalence of oral candidiasis was higher in untreated than in HAART-treated HIV-positive patients (16% vs. 2%; p  < 0.01). Oral candidiasis was furthermore associated with high fungal burdens of Candida albicans and a CD4 + T-cell number <200/μl. A shift toward non -albicans Candida species was observed under nucleoside-based HAART therapy. Azole antifungal drug resistance was only observed for the intrinsically resistant species Candida krusei and Candida glabrata . Prevalence of oral candidiasis in the studied area was very low. The species distribution was similar to other countries around the world, with C. albicans being dominant. Candida dubliniensis was not isolated. Nucleoside-based HAART therapy significantly reduced oral colonization as well as occurrence of oral candidiasis caused by C. albicans and led to a species shift toward non- albicans species. Antifungal resistance was not yet a concern in Chad."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3389/fmicb.2022.844069"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105503"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Epidemiology and Prevalence of Oral Candidiasis in HIV Patients From Chad in the Post-HAART Era"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e70425"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Morais, Janne K. S."],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Oliveira, Jose Tadeu A."],["dc.contributor.author","Arantes, Mariana R."],["dc.contributor.author","Gomes, Valdirene M."],["dc.contributor.author","Da Cunha, Maura"],["dc.contributor.author","Oliveira, Hermogenes D."],["dc.contributor.author","Sousa, Daniele O. B."],["dc.contributor.author","Lourencao, Andre L."],["dc.contributor.author","Vasconcelos, Ilka M."],["dc.date.accessioned","2018-11-07T09:22:19Z"],["dc.date.available","2018-11-07T09:22:19Z"],["dc.date.issued","2013"],["dc.description.abstract","Soybean toxin (SBTX) is an antifungal protein from soybeans with broad inhibitory activity against the growth and filamentation of many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina and Cercospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute to the design of novel antifungal drugs and/or the development of transgenic plants resistant to pathogens. To this end, the polymorphic yeast C. albicans was chosen as a model organism and changes in the gene expression profile of strain SC5314 upon exposure to SBTX were examined. Genes that were differentially regulated in the presence of SBTX were involved in glucose transport and starvation-associated stress responses as well as in the control of both the induction and repression of C. albicans hyphal formation. Transmission electron microscopy showed that C. albicans cells exposed to SBTX displayed severe signs of starvation and were heavily granulated. Our data were indicative of C. albicans cell starvation despite sufficient nutrient availability in the medium; therefore, it can be speculated that SBTX blocks nutrient uptake systems. Because neither the starvation signal nor the alkaline response pathway lead to the induction of hyphae, we hypothesise that conflicting signals are transmitted to the complex regulatory network controlling morphogenesis, eventually preventing the filamentation signal from reaching a significant threshold."],["dc.identifier.doi","10.1371/journal.pone.0070425"],["dc.identifier.isi","000322132100094"],["dc.identifier.pmid","23894655"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29314"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Cellular and Infection Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Bernhard, Mareike"],["dc.contributor.author","Worasilchai, Navaporn"],["dc.contributor.author","Kangogo, Mourine"],["dc.contributor.author","Bii, Christine"],["dc.contributor.author","Trzaska, Wioleta J."],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Chindamporn, Ariya"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2021-06-01T09:42:23Z"],["dc.date.available","2021-06-01T09:42:23Z"],["dc.date.issued","2021"],["dc.description.abstract","Yeasts of the Cryptococcus neoformans / gattii species complexes are human pathogens mostly in immune compromised individuals, and can cause infections from dermal lesions to fungal meningitis. Differences in virulence and antifungal drug susceptibility of species in these complexes indicate the value of full differentiation to species level in diagnostic procedures. MALDI-TOF MS has been reported to sufficiently discriminate these species. Here, we sought to re-evaluate sample pre-processing procedures and create a set of publicly available references for use with the MALDI Biotyper system. Peak content using four different pre-processing protocols was assessed, and database entries for 13 reference strains created. These were evaluated against a collection of 153 clinical isolates, typed by conventional means. The use of decapsulating protocols or mechanical disruption did not sufficiently increase the information content to justify the extra hands-on-time. Using the set of 13 reference entries created with the standard formic acid extraction, we were able to correctly classify 143/153 (93.5%) of our test isolates. The majority of the remaining ten isolates still gave correct top matches; only two isolates did not give reproducible identifications. This indicates that the log score cut-off can be lowered also in this context. Ease to identify cryptococcal isolates to the species level is improved by the workflow evaluated here. The database references are freely available from https://github.com/oliverbader/BioTyper-libraries for incorporation into local diagnostic systems."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3389/fcimb.2021.634382"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85236"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2235-2988"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","CryptoType – Public Datasets for MALDI-TOF-MS Based Differentiation of Cryptococcus neoformans/gattii Complexes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","501"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Vaz, Catarina"],["dc.contributor.author","Pitarch, Aida"],["dc.contributor.author","Gómez-Molero, Emilia"],["dc.contributor.author","Amador-García, Ahinara"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Monteoliva, Lucía"],["dc.contributor.author","Gil, Concha"],["dc.date.accessioned","2021-09-01T06:43:05Z"],["dc.date.available","2021-09-01T06:43:05Z"],["dc.date.issued","2021"],["dc.description.abstract","Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests."],["dc.description.abstract","Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests."],["dc.description.sponsorship","European Commission"],["dc.description.sponsorship","Ministerio de Economía, Industria y Competitividad, Gobierno de España"],["dc.description.sponsorship","Comunidad de Madrid"],["dc.description.sponsorship","Instituto de Salud Carlos III"],["dc.description.sponsorship","European Regional Development Fund"],["dc.identifier.doi","10.3390/jof7070501"],["dc.identifier.pii","jof7070501"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89215"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.publisher","MDPI"],["dc.relation.eissn","2309-608X"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e52218"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Schwarz, Alexander"],["dc.contributor.author","Kraneveld, Eefje A."],["dc.contributor.author","Tangwattanchuleeporn, Marut"],["dc.contributor.author","Schmidt, Pia"],["dc.contributor.author","Jacobsen, Mette D."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","de Groot, Piet W. J."],["dc.contributor.author","Weig, Michael S."],["dc.date.accessioned","2018-11-07T09:02:13Z"],["dc.date.available","2018-11-07T09:02:13Z"],["dc.date.issued","2012"],["dc.description.abstract","Genomic plasticity is a mechanism for adaptation to environmental cues such as host responses and antifungal drug pressure in many fungi including the human pathogenic yeast Candida glabrata. In this study we evaluated the phenotypic and genotypic stability of the world-wide used C. glabrata reference strain CBS138/ ATCC2001 under laboratory conditions. A set of ten lineages of this wild type strain and genetically modified progenies were obtained from different scientific laboratories, and analyzed for genotypic and phenotypic alterations. Even though the derivates were indistinguishable by multi locus sequence typing, different phenotypic groups that correlated with specific karyotypic changes were observed. In addition, modifications in the adherence capacity to plastic surface emerged that were shown to correlate with quantitative changes in adhesin gene expression rather than subtelomeric gene loss or differences in the number of macrosatellite repeats within adhesin genes. These results confirm the genomic plasticity of C. glabrata and show that chromosomal aberrations and functional adaptations may occur not only during infection and under antimicrobial therapy, but also under laboratory conditions without extreme selective pressures. These alterations can significantly affect phenotypic properties such as cell surface attributes including adhesion and the cell wall carbohydrate composition and therefore, if unnoticed, may adulterate the outcome of genetic studies."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1371/journal.pone.0052218"],["dc.identifier.isi","000312794500117"],["dc.identifier.pmid","23284942"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8472"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24630"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Gross Karyotypic and Phenotypic Alterations among Different Progenies of the Candida glabrata CBS138/ATCC2001 Reference Strain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2239"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","14"],["dc.contributor.affiliation","Dierks, Sascha; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Thiele, Karin; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Bohne, Wolfgang; 2Interdisciplinary UMG Laboratory, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Lugert, Raimond; 2Interdisciplinary UMG Laboratory, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Weig, Michael; 2Interdisciplinary UMG Laboratory, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Groß, Uwe; 2Interdisciplinary UMG Laboratory, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","von Ahsen, Nicolas; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Schanz, Julie; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Fischer, Andreas; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Schnelle, Moritz; 1Department of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany"],["dc.contributor.author","Dierks, Sascha"],["dc.contributor.author","Thiele, Karin"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Schanz, Julie"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Schnelle, Moritz"],["dc.date.accessioned","2022-12-01T08:31:48Z"],["dc.date.available","2022-12-01T08:31:48Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:11:59Z"],["dc.description.abstract","In SARS-CoV-2 diagnostics, cycle threshold (Ct) values from qRT-PCRs semi-quantitatively estimate a patient’s viral load. However, relevant analytical differences between qRT-PCR assays are often neglected. This study was designed (i) to identify such differences between five commonly used assays and (ii) to demonstrate a straightforward strategy to harmonize them. QRT-PCRs for SARS-CoV-2 were carried out in 85 oropharyngeal swab samples using three fully automated (Alinity m, cobas®6800 and GeneXpert) and two semi-automated (genesig® and RIDA®GENE) assays. Qualitative results (positive/negative) showed excellent comparability between the fully automated assays, but not between the Alinity m and semi-automated methods. Ct values significantly varied between all the methods, with the median values ranging from 22.76 (Alinity m) to 30.89 (RIDA®GENE) and 31.50 (genesig®), indicating the lowest sensitivity for semi-automated methods. Passing–Bablok analysis further revealed systemic biases. Assay-specific viral load concentration calculations—based on generated individual standard curves—resulted in much better comparability between the assays. Applying these calculations, significant differences were no longer detectable. This study highlights relevant analytical differences between SARS-CoV-2 qRT-PCR assays, leading to divergent decisions about the mandatory isolation of infected individuals. Secondly, we propose a strategy to harmonize qRT-PCR assays to achieve better comparability. Our findings are of particular interest for laboratories utilizing different assays."],["dc.description.sponsorship","VolkswagenStiftung"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3390/v14102239"],["dc.identifier.pii","v14102239"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118269"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1999-4915"],["dc.rights","CC BY 4.0"],["dc.title","Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","unpublished"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","258"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Mühlhausen, Stefanie"],["dc.contributor.author","Plessmann, Uwe"],["dc.contributor.author","Mienkus, Peter"],["dc.contributor.author","Sternisek, Pia"],["dc.contributor.author","Perl, Thorsten"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Kollmar, Martin"],["dc.contributor.author","Schmitt, Hans Dieter"],["dc.date.accessioned","2022-01-11T14:06:08Z"],["dc.date.accessioned","2022-08-18T12:34:34Z"],["dc.date.available","2022-01-11T14:06:08Z"],["dc.date.available","2022-08-18T12:34:34Z"],["dc.date.issued","2021-12-04"],["dc.date.updated","2022-07-29T12:07:10Z"],["dc.description.abstract","Abstract\r\n \r\n Background\r\n Yeasts of the CTG-clade lineage, which includes the human-infecting Candida albicans, Candida parapsilosis and Candida tropicalis species, are characterized by an altered genetic code. Instead of translating CUG codons as leucine, as happens in most eukaryotes, these yeasts, whose ancestors are thought to have lost the relevant leucine-tRNA gene, translate CUG codons as serine using a serine-tRNA with a mutated anticodon, \r\n \r\n \r\n \r\n \r\n \r\n tRNA\r\n CAG\r\n Ser\r\n \r\n \r\n $ {\\mathrm{tRNA}}_{\\mathrm{CAG}}^{\\mathrm{Ser}} $\r\n . Previously reported experiments have suggested that 3–5% of the CTG-clade CUG codons are mistranslated as leucine due to mischarging of the \r\n \r\n \r\n \r\n \r\n \r\n tRNA\r\n CAG\r\n Ser\r\n \r\n \r\n $ {\\mathrm{tRNA}}_{\\mathrm{CAG}}^{\\mathrm{Ser}} $\r\n . The mistranslation was suggested to result in variable surface proteins explaining fast host adaptation and pathogenicity.\r\n \r\n \r\n Results\r\n In this study, we reassess this potential mistranslation by high-resolution mass spectrometry-based proteogenomics of multiple CTG-clade yeasts, including various C. albicans strains, isolated from colonized and from infected human body sites, and C. albicans grown in yeast and hyphal forms. Our data do not support a bias towards CUG codon mistranslation as leucine. Instead, our data suggest that (i) CUG codons are mistranslated at a frequency corresponding to the normal extent of ribosomal mistranslation with no preference for specific amino acids, (ii) CUG codons are as unambiguous (or ambiguous) as the related CUU leucine and UCC serine codons, (iii) tRNA anticodon loop variation across the CTG-clade yeasts does not result in any difference of the mistranslation level, and (iv) CUG codon unambiguity is independent of C. albicans’ strain pathogenicity or growth form.\r\n \r\n \r\n Conclusions\r\n Our findings imply that C. albicans does not decode CUG ambiguously. This suggests that the proposed misleucylation of the \r\n \r\n \r\n \r\n \r\n \r\n tRNA\r\n CAG\r\n Ser\r\n \r\n \r\n $ {\\mathrm{tRNA}}_{\\mathrm{CAG}}^{\\mathrm{Ser}} $\r\n might be as prevalent as every other misacylation or mistranslation event and, if at all, be just one of many reasons causing phenotypic diversity."],["dc.identifier.citation","BMC Biology. 2021 Dec 04;19(1):258"],["dc.identifier.doi","10.1186/s12915-021-01197-9"],["dc.identifier.pii","1197"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97834"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112931"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.publisher","BioMed Central"],["dc.relation.eissn","1741-7007"],["dc.rights.holder","The Author(s)"],["dc.subject","Proteogenomics"],["dc.subject","Pathogen"],["dc.subject","Candida albicans"],["dc.subject","Genetic code"],["dc.title","Proteogenomics analysis of CUG codon translation in the human pathogen Candida albicans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","13431"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:53:08Z"],["dc.date.available","2018-11-07T09:53:08Z"],["dc.date.issued","2015"],["dc.description.abstract","MALDI-TOF-MS of microorganisms, which identifies microbes based on masses of high abundant low molecular weight proteins, is rapidly advancing to become another standard method in clinical routine laboratory diagnostics. Allelic isoforms of these proteins result in varying masses of detectable biomarker ions. These variations give rise to a novel typing method for microorganisms named mass spectrometry-based phyloproteomics (MSPP). The base of MSPP is an amino acid sequence list of allelic isoforms caused by non-synonymous mutations in biomarker genes, which were detectable as mass shifts in an overlay of calibrated MALDI-TOF spectra. Thus, for each isolate a combination of amino acid sequences can be deduced from the scheme of recordable biomarker masses. Performing comparably to laborious multilocus and whole genome sequence typing (wgMLST)-approaches it is feasible to build phyloproteomic dendrograms using hierarchical cluster analysis. MSPP bears a high potential especially for identification of chromosomal localised virulence or antimicrobial resistance factors associated with evolutionary relatedness. In this study the principle of MSPP-typing was demonstrated on a Campylobacter jejuni ssp. jejuni isolate collection and MSPP was compared to MLST."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft; Georg August Universitat Gottingen"],["dc.identifier.doi","10.1038/srep13431"],["dc.identifier.isi","000360037600002"],["dc.identifier.pmid","26303099"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12455"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36271"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Mass Spectrometry-based PhyloProteomics (MSPP): A novel microbial typing Method"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Gómez-Molero, Emilia"],["dc.contributor.author","Willis, Jesse R."],["dc.contributor.author","Dudakova, Anna"],["dc.contributor.author","Carreté, Laia"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Gácser, Attila"],["dc.contributor.author","Gabaldón, Toni"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2021-04-14T08:32:38Z"],["dc.date.available","2021-04-14T08:32:38Z"],["dc.date.issued","2020"],["dc.description.abstract","The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population of C. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient’s nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly in HWP1 of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination."],["dc.identifier.doi","10.3389/fmicb.2020.01994"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17528"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83971"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e50518"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Wagener, Jeanette"],["dc.contributor.author","Weindl, Guenther"],["dc.contributor.author","de Groot, Piet W. J."],["dc.contributor.author","de Boer, Albert D."],["dc.contributor.author","Kaesler, Susanne"],["dc.contributor.author","Thavaraj, Selvam"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Mailaender-Sanchez, Daniela"],["dc.contributor.author","Borelli, Claudia"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Biedermann, Tilo"],["dc.contributor.author","Naglik, Julian R."],["dc.contributor.author","Korting, Hans Christian"],["dc.contributor.author","Schaller, Martin"],["dc.date.accessioned","2018-11-07T09:03:11Z"],["dc.date.available","2018-11-07T09:03:11Z"],["dc.date.issued","2012"],["dc.description.abstract","C. albicans is one of the most common fungal pathogen of humans, causing local and superficial mucosal infections in immunocompromised individuals. Given that the key structure mediating host-C. albicans interactions is the fungal cell wall, we aimed to identify features of the cell wall inducing epithelial responses and be associated with fungal pathogenesis. We demonstrate here the importance of cell wall protein glycosylation in epithelial immune activation with a predominant role for the highly branched N-glycosylation residues. Moreover, these glycan moieties induce growth arrest and apoptosis of epithelial cells. Using an in vitro model of oral candidosis we demonstrate, that apoptosis induction by C. albicans wild-type occurs in early stage of infection and strongly depends on intact cell wall protein glycosylation. These novel findings demonstrate that glycosylation of the C. albicans cell wall proteins appears essential for modulation of epithelial immunity and apoptosis induction, both of which may promote fungal pathogenesis in vivo."],["dc.identifier.doi","10.1371/journal.pone.0050518"],["dc.identifier.isi","000312376100113"],["dc.identifier.pmid","23226301"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8406"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24852"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Glycosylation of Candida albicans Cell Wall Proteins is Critical for Induction of Innate Immune Responses and Apoptosis of Epithelial Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]