Schulz-Schaeffer, Walter Joachim

[["dc.bibliographiccitation.firstpage","758"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of neuropathology and experimental neurology"],["dc.bibliographiccitation.lastpage","767"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Wemheuer, Wiebke M."],["dc.contributor.author","Wrede, Arne"],["dc.contributor.author","Gawinecka, Joanna"],["dc.contributor.author","Zerr, Inga"],["dc.contributor.author","Schulz-Schaeffer, Walter J."],["dc.date.accessioned","2015-11-18T10:28:52Z"],["dc.date.accessioned","2021-10-27T13:20:22Z"],["dc.date.available","2015-11-18T10:28:52Z"],["dc.date.available","2021-10-27T13:20:22Z"],["dc.date.issued","2013-08-01"],["dc.description.abstract","In brain biopsies taken from patients with rapidly progressive dementia, the first differential diagnoses to be ruled out are prion diseases. For safe diagnostic processing of tissue and instruments, a rapid, highly sensitive, and specific analysis for prion aggregates is necessary. Here, we examined 16 brain biopsies and brain samples (frontal cortex and cerebellum) from 65 autopsies by Western blot, paraffin-embedded tissue (PET) blot, immunohistochemistry, and the recently described membrane adsorption assay (MAA) for their suitability to detect pathologic prion protein. In our hands, the PET blot method provided the highest sensitivity in prion detection (biopsies, 100%; all autopsy sections, 96.3%), closely followed by the MAA (biopsies, 100%; all autopsy samples, 96%) and Western blot analysis (biopsies, 100%; all autopsy samples, 92%). Conventional immunohistochemistry is the least sensitive method (biopsies, 50%; all autopsy sections, 80%) and also gave 1 false-positive biopsy result. Consequently, our standard diagnostic protocol is to use the MAA as a first step for detecting or excluding a prion disease, followed by the PET blot for the prion deposition pattern, Western blotting for prion typing, and immunohistochemistry for differential diagnoses. With this standard and the availability of unfixed tissue, a diagnosis was possible in all 16 biopsies examined."],["dc.description.sponsorship","VolkswagenStiftung [VWZ2168]; Prionscreen [FP6-2005-SSP-5A]"],["dc.format.extent","19"],["dc.identifier.doi","10.1097/NEN.0b013e31829d2799"],["dc.identifier.isi","000330383600005"],["dc.identifier.pmid","23860029"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12445"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91960"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1554-6578"],["dc.relation.issn","0022-3069"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Alzheimer Disease"],["dc.subject.mesh","Biopsy"],["dc.subject.mesh","Brain"],["dc.subject.mesh","Cell Membrane"],["dc.subject.mesh","Dementia"],["dc.subject.mesh","Female"],["dc.subject.mesh","Filtration"],["dc.subject.mesh","Frontotemporal Lobar Degeneration"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Male"],["dc.subject.mesh","Parkinson Disease"],["dc.subject.mesh","Positron-Emission Tomography"],["dc.subject.mesh","PrPSc Proteins"],["dc.subject.mesh","Prion Diseases"],["dc.subject.mesh","Prions"],["dc.subject.mesh","Sensitivity and Specificity"],["dc.title","Filtration of protein aggregates increases the accuracy for diagnosing prion diseases in brain biopsies."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","677"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","The Veterinary record"],["dc.bibliographiccitation.lastpage","681"],["dc.bibliographiccitation.volume","164"],["dc.contributor.author","Wemheuer, W. M."],["dc.contributor.author","Benestad, S. L."],["dc.contributor.author","Wrede, A."],["dc.contributor.author","Wemheuer, W. E."],["dc.contributor.author","Brenig, B."],["dc.contributor.author","Bratberg, B."],["dc.contributor.author","Schulz-Schaeffer, W. J."],["dc.date.accessioned","2021-11-22T14:31:26Z"],["dc.date.available","2021-11-22T14:31:26Z"],["dc.date.issued","2009"],["dc.description.abstract","The paraffin-embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP(Sc) in the brain tissue of all 49 sheep with scrapie but no PrP(Sc) labelling could be detected in the control sheep. By contrast, not all the atypical/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical/Nor98 cases, deposits of PrP(Sc) may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical/Nor98 cases."],["dc.identifier.doi","10.1136/vr.164.22.677"],["dc.identifier.isi","000266753400009"],["dc.identifier.pmid","19483208"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10436"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/93378"],["dc.language","eng"],["dc.language.iso","en"],["dc.notes","This is the accepted, peer-reviewed manuscript of an article whose final and definitive form has been\r\npublished in The Veterinary record, Vol. 164, Nr. 22, p. 677-81: http://veterinaryrecord.bmj.com/content/164/22/677.long"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bmj Publishing Group"],["dc.relation.issn","0042-4900"],["dc.rights.access","openAccess"],["dc.subject","Animals; Blotting, Western/methods; Blotting, Western/veterinary*; Brain/pathology; Case-Control Studies; Central Nervous System/pathology; Immunohistochemistry/veterinary; Lymphoid Tissue/pathology; Palatine Tonsil/pathology; Paraffin Embedding/methods*; Prions/genetics; Prions/isolation & purification*; Scrapie/genetics; Scrapie/pathology*; Sensitivity and Specificity; Sheep; Prions"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Blotting, Western"],["dc.subject.mesh","Brain"],["dc.subject.mesh","Case-Control Studies"],["dc.subject.mesh","Central Nervous System"],["dc.subject.mesh","Immunohistochemistry"],["dc.subject.mesh","Lymphoid Tissue"],["dc.subject.mesh","Palatine Tonsil"],["dc.subject.mesh","Paraffin Embedding"],["dc.subject.mesh","Prions"],["dc.subject.mesh","Scrapie"],["dc.subject.mesh","Sensitivity and Specificity"],["dc.subject.mesh","Sheep"],["dc.title","Detection of classical and atypical/Nor98 scrapie by the paraffin-embedded tissue blot method."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]