Buttler, Kerstin

[["dc.bibliographiccitation.firstpage","163"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Angiogenesis"],["dc.bibliographiccitation.lastpage","172"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Hemmen, Katherina"],["dc.contributor.author","Reinl, Tobias"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Behler, Friederike"],["dc.contributor.author","Dieken, Hauke"],["dc.contributor.author","Jaensch, Lothar"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Weich, Herbert A."],["dc.date.accessioned","2018-11-07T08:56:20Z"],["dc.date.available","2018-11-07T08:56:20Z"],["dc.date.issued","2011"],["dc.description.abstract","Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogeneis."],["dc.identifier.doi","10.1007/s10456-011-9200-x"],["dc.identifier.isi","000291038200006"],["dc.identifier.pmid","21234671"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23121"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0969-6970"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","50"],["dc.bibliographiccitation.journal","BMC Cell Biology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schniedermann, Judith"],["dc.contributor.author","Rennecke, Moritz"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Richter, Georg"],["dc.contributor.author","Staedtler, Anna-Maria"],["dc.contributor.author","Norgall, Susanne"],["dc.contributor.author","Badar, Muhammad"],["dc.contributor.author","Barleon, Bernhard"],["dc.contributor.author","May, Tobias"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Weich, Herbert A."],["dc.date.accessioned","2018-11-07T08:41:30Z"],["dc.date.available","2018-11-07T08:41:30Z"],["dc.date.issued","2010"],["dc.description.abstract","Background: Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated. Results: In an attempt to isolate differentiated mature endothelial cells from mouse lung we found that the lung contains EPCs with a high vasculogenic capacity and capability of de novo vasculogenesis for blood and lymph vessels. Mouse lung microvascular endothelial cells (MLMVECs) were isolated by selection of CD31(+) cells. Whereas the majority of the CD31+ cells did not divide, some scattered cells started to proliferate giving rise to large colonies (> 3000 cells/colony). These highly dividing cells possess the capacity to integrate into various types of vessels including blood and lymph vessels unveiling the existence of local microvascular endothelial progenitor cells (LMEPCs) in adult mouse lung. EPCs could be amplified > passage 30 and still expressed panendothelial markers as well as the progenitor cell antigens, but not antigens for immune cells and hematopoietic stem cells. A high percentage of these cells are also positive for Lyve1, Prox1, podoplanin and VEGFR-3 indicating that a considerabe fraction of the cells are committed to develop lymphatic endothelium. Clonogenic highly proliferating cells from limiting dilution assays were also bipotent. Combined in vitro and in vivo spheroid and matrigel assays revealed that these EPCs exhibit vasculogenic capacity by forming functional blood and lymph vessels. Conclusion: The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell."],["dc.identifier.doi","10.1186/1471-2121-11-50"],["dc.identifier.isi","000282731800001"],["dc.identifier.pmid","20594323"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19484"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2121"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]