Schilling, Arndt Friedrich

[["dc.bibliographiccitation.artnumber","e0174860"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Perisic, Tatjana"],["dc.contributor.author","Zhang, Z."],["dc.contributor.author","Foehr, Peter"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Klutz, Kathrin"],["dc.contributor.author","Burgkart, Rainer H."],["dc.contributor.author","Slobodianski, Alexei"],["dc.contributor.author","Goeldner, Moritz"],["dc.contributor.author","Machens, Hans-Gunther"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2018-11-07T10:25:06Z"],["dc.date.available","2018-11-07T10:25:06Z"],["dc.date.issued","2017"],["dc.description.abstract","Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine."],["dc.identifier.doi","10.1371/journal.pone.0174860"],["dc.identifier.isi","000399353500069"],["dc.identifier.pmid","28380080"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42784"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for geneticallymodified fibroblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","22"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Functional Biomaterials"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hadjipanayi, Ektoras"],["dc.contributor.author","Moog, Philipp"],["dc.contributor.author","Bekeran, Sanjar"],["dc.contributor.author","Kirchhoff, Katharina"],["dc.contributor.author","Berezhnoi, Andrei"],["dc.contributor.author","Aguirre, Juan"],["dc.contributor.author","Bauer, Anna-Theresa"],["dc.contributor.author","Kükrek, Haydar"],["dc.contributor.author","Schmauss, Daniel"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Isenburg, Sarah"],["dc.contributor.author","Ntziachristos, Vasilis"],["dc.contributor.author","Ninkovic, Milomir"],["dc.contributor.author","Machens, Hans-Günther"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Dornseifer, Ulf"],["dc.date.accessioned","2020-12-10T18:47:13Z"],["dc.date.available","2020-12-10T18:47:13Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3390/jfb10020022"],["dc.identifier.eissn","2079-4983"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78685"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2079-4983"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","In Vitro Characterization of Hypoxia Preconditioned Serum (HPS)—Fibrin Hydrogels: Basis for an Injectable Biomimetic Tissue Regeneration Therapy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","60"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Medical Research"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Cerny, Michael K"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Kirsch, Manuela"],["dc.contributor.author","Haas, Elisabeth-Maria"],["dc.contributor.author","Wu, Fan"],["dc.contributor.author","Giunta, Riccardo"],["dc.contributor.author","Machens, Hans-Guenther"],["dc.contributor.author","Duscher, Dominik"],["dc.contributor.author","Erne, Holger"],["dc.contributor.author","Schilling, Arndt F"],["dc.date.accessioned","2019-07-09T11:49:46Z"],["dc.date.available","2019-07-09T11:49:46Z"],["dc.date.issued","2018"],["dc.description.abstract","Abstract Background Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. Methods We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60 h and by Alamarblue analysis after 72 h. Cell migration was evaluated continuously for 15 h and confirmed by the in vitro wound-healing assay. Results Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6 h after incubation and reversed after 9 h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10 h and was significantly decreased after 45 h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. Conclusion OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation."],["dc.identifier.doi","10.1186/s40001-018-0357-2"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15764"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59626"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","BioMed Central"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5665"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Journal of Cellular and Molecular Medicine"],["dc.bibliographiccitation.lastpage","5674"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Grünherz, Lisanne"],["dc.contributor.author","Prein, Carina"],["dc.contributor.author","Winkler, Thomas"],["dc.contributor.author","Kirsch, Manuela"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Streichert, Thomas"],["dc.contributor.author","Clausen‐Schaumann, Hauke"],["dc.contributor.author","Zustin, Jozef"],["dc.contributor.author","Kirchhof, Kristin"],["dc.contributor.author","Morlock, Michael M."],["dc.contributor.author","Machens, Hans‐Günter"],["dc.contributor.author","Schilling, Arndt Friedrich"],["dc.date.accessioned","2021-04-14T08:26:35Z"],["dc.date.available","2021-04-14T08:26:35Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1111/jcmm.15227"],["dc.identifier.eissn","1582-4934"],["dc.identifier.issn","1582-1838"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17453"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82004"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1582-4934"],["dc.relation.issn","1582-1838"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Osteoidosis leads to altered differentiation and function of osteoclasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e368"],["dc.bibliographiccitation.journal","NPG Asia Materials"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Dai, Xinyi"],["dc.contributor.author","Liu, Juan"],["dc.contributor.author","Zheng, Huaiyuan"],["dc.contributor.author","Wichmann, Johannes"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Sudhop, Stefanie"],["dc.contributor.author","Prein, Carina"],["dc.contributor.author","Shen, Y. I."],["dc.contributor.author","Machens, Hans-Guenther"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2018-11-07T10:26:47Z"],["dc.date.available","2018-11-07T10:26:47Z"],["dc.date.issued","2017"],["dc.description.abstract","Turmeric, a product of Curcuma longa, has a very long history of being used for the treatment of wounds in many Asian countries. Curcumin, the principal curcuminoid of turmeric, has recently been identified as a main mediator of turmeric's medicinal properties. However, the inherent limitations of the compound itself, such as hydrophobicity, instability, poor absorption and rapid systemic elimination, pose big hurdles for translation to wider clinical application. We present here an approach for engineering curcumin/gelatin-blended nanofibrous mats (NMs) by electrospinning to adequately enhance the bioavailability of the hydrophobic curcumin for wound repair. Curcumin was successfully formulated as an amorphous nanosolid dispersion and favorably released from gelatin-based biomimetic NMs that could be easily applied topically to experimental wounds. We show synergistic signaling by the released curcumin during the healing process: (i) mobilization of wound site fibroblasts by activating the Wnt signaling pathway, partly mediated through Dickkopf-related protein-1, and (ii) persistent inhibition of the inflammatory response through decreased expression of monocyte chemoattractant protein-1 by fibroblasts. With a combination of these effects, the curcumin/gelatin-blended NMs enhanced the regenerative process in a rat model of acute wounds, providing a method for translating this ancient medicine for use in modern wound therapy."],["dc.identifier.doi","10.1038/am.2017.31"],["dc.identifier.isi","000397902700003"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14950"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43117"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1884-4057"],["dc.relation.issn","1884-4049"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","798"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","International Journal of Medical Sciences"],["dc.bibliographiccitation.lastpage","803"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Zhang, Ziyang"],["dc.contributor.author","Slobodianski, Alex"],["dc.contributor.author","Arnold, Astrid"],["dc.contributor.author","Nehlsen, Jessica"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Perisic, Tatjana"],["dc.contributor.author","Machens, Hans-Günther"],["dc.date.accessioned","2020-12-10T18:48:03Z"],["dc.date.available","2020-12-10T18:48:03Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.7150/ijms.19241"],["dc.identifier.issn","1449-1907"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79001"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]