Schilling, Arndt Friedrich

[["dc.bibliographiccitation.firstpage","681"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Engineering in Life Sciences"],["dc.bibliographiccitation.lastpage","698"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Fattahi, Ehsan"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Becker, Thomas"],["dc.contributor.author","Pörtner, Ralf"],["dc.date.accessioned","2022-12-01T08:31:00Z"],["dc.date.available","2022-12-01T08:31:00Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1002/elsc.202100128"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118042"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1618-2863"],["dc.relation.issn","1618-0240"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Generation and evaluation of input values for computational analysis of transport processes within tissue cultures"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Immunology"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Rösch, Gundula"],["dc.contributor.author","Muschter, Dominique"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","El Bagdadi, Karima"],["dc.contributor.author","Dorn, Christoph"],["dc.contributor.author","Meurer, Andrea"],["dc.contributor.author","Zaucke, Frank"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Grässel, Susanne"],["dc.contributor.author","Straub, Rainer H."],["dc.contributor.author","Jenei-Lanzl, Zsuzsa"],["dc.date.accessioned","2022-04-01T10:00:50Z"],["dc.date.available","2022-04-01T10:00:50Z"],["dc.date.issued","2022"],["dc.description.abstract","Purpose Recent studies demonstrated a contribution of adrenoceptors (ARs) to osteoarthritis (OA) pathogenesis. Several AR subtypes are expressed in joint tissues and the β2-AR subtype seems to play a major role during OA progression. However, the importance of β2-AR has not yet been investigated in knee OA. Therefore, we examined the development of knee OA in β2-AR-deficient ( Adrb2 -/- ) mice after surgical OA induction. Methods OA was induced by destabilization of the medial meniscus (DMM) in male wildtype (WT) and Adrb2 -/- mice. Cartilage degeneration and synovial inflammation were evaluated by histological scoring. Subchondral bone remodeling was analyzed using micro-CT. Osteoblast (alkaline phosphatase - ALP) and osteoclast (cathepsin K - CatK) activity were analyzed by immunostainings. To evaluate β2-AR deficiency-associated effects, body weight, sympathetic tone (splenic norepinephrine (NE) via HPLC) and serum leptin levels (ELISA) were determined. Expression of the second major AR, the α2-AR, was analyzed in joint tissues by immunostaining. Results WT and Adrb2 -/- DMM mice developed comparable changes in cartilage degeneration and synovial inflammation. Adrb2 -/- DMM mice displayed elevated calcified cartilage and subchondral bone plate thickness as well as increased epiphyseal BV/TV compared to WTs, while there were no significant differences in Sham animals. In the subchondral bone of Adrb2 -/- mice, osteoblasts activity increased and osteoclast activity deceased. Adrb2 -/- mice had significantly higher body weight and fat mass compared to WT mice. Serum leptin levels increased in Adrb2 -/- DMM compared to WT DMM without any difference between the respective Shams. There was no difference in the development of meniscal ossicles and osteophytes or in the subarticular trabecular microstructure between Adrb2 -/- and WT DMM as well as Adrb2 -/- and WT Sham mice. Number of α2-AR-positive cells was lower in Adrb2 -/- than in WT mice in all analyzed tissues and decreased in both Adrb2 -/- and WT over time. Conclusion We propose that the increased bone mass in Adrb2 -/- DMM mice was not only due to β2-AR deficiency but to a synergistic effect of OA and elevated leptin concentrations. Taken together, β2-AR plays a major role in OA-related subchondral bone remodeling and is thus an attractive target for the exploration of novel therapeutic avenues."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3389/fimmu.2021.801505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105526"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1664-3224"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","115181"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Muschter, Dominique"],["dc.contributor.author","Fleischhauer, Lutz"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Clausen-Schaumann, Hauke"],["dc.contributor.author","Grässel, Susanne"],["dc.date.accessioned","2020-12-10T14:22:41Z"],["dc.date.available","2020-12-10T14:22:41Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1016/j.bone.2019.115181"],["dc.identifier.issn","8756-3282"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71693"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Sensory neuropeptides are required for bone and cartilage homeostasis in a murine destabilization-induced osteoarthritis model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","13282"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Shang, Xiaobin"],["dc.contributor.author","Böker, Kai Oliver"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2022-02-01T10:31:49Z"],["dc.date.available","2022-02-01T10:31:49Z"],["dc.date.issued","2021"],["dc.date.updated","2022-09-03T17:10:22Z"],["dc.description.abstract","MicroRNAs (miRNAs) can be transported in extracellular vesicles (EVs) and are qualified as possible messengers for cell–cell communication. In the context of osteoarthritis (OA), miR-221-3p has been shown to have a mechanosensitive and a paracrine function inside cartilage. However, the question remains if EVs with miR-221-3p can act as molecular mechanotransducers between cells of different tissues. Here, we studied the effect of EV-mediated transport in the communication between chondrocytes and osteoblasts in vitro in a rat model. In silico analysis (Targetscan, miRWalk, miRDB) revealed putative targets of miRNA-221-3p (CDKN1B/p27, TIMP-3, Tcf7l2/TCF4, ARNT). Indeed, transfection of miRNA-221-3p in chondrocytes and osteoblasts resulted in regulation of these targets. Coculture experiments of transfected chondrocytes with untransfected osteoblasts not only showed regulation of these target genes in osteoblasts but also inhibition of their bone formation capacity. Direct treatment with chondrocyte-derived EVs validated that chondrocyte-produced extracellular miR-221-3p was responsible for this effect. Altogether, our study provides a novel perspective on a possible communication pathway of a mechanically induced epigenetic signal through EVs. This may be important for processes at the interface of bone and cartilage, such as OA development, physiologic joint homeostasis, growth or fracture healing, as well as for other tissue interfaces with differing biomechanical properties."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/ijms222413282"],["dc.identifier.pii","ijms222413282"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98954"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1422-0067"],["dc.rights","CC BY 4.0"],["dc.title","Extracellular Vesicles Allow Epigenetic Mechanotransduction between Chondrocytes and Osteoblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","770"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","20"],["dc.contributor.affiliation","Taheri, Shahed; \t\t \r\n\t\t Clinic for Trauma Surgery, Orthopaedic Surgery and Plastic Surgery, Universitätsmedizin Göttingen, 37075 Göttingen, Germany, shahed.taheri@med.uni-goettingen.de"],["dc.contributor.affiliation","Winkler, Thomas; \t\t \r\n\t\t Institute of Biomechanics, Technische Universität Hamburg-Harburg, 21073 Hamburg, Germany, thomas.winkler01@gmail.com"],["dc.contributor.affiliation","Schenk, Lia Sabrina; \t\t \r\n\t\t Institute of Biomechanics, Technische Universität Hamburg-Harburg, 21073 Hamburg, Germany, l.schenk@implantcast.de"],["dc.contributor.affiliation","Neuerburg, Carl; \t\t \r\n\t\t Experimental Surgery and Regenerative Medicine, Department of Trauma Surgery−Campus Innenstadt, Munich University Hospital LMU, 80336 Munich, Germany, carl.neuerburg@med.uni-muenchen.de"],["dc.contributor.affiliation","Baumbach, Sebastian Felix; \t\t \r\n\t\t Experimental Surgery and Regenerative Medicine, Department of Trauma Surgery−Campus Innenstadt, Munich University Hospital LMU, 80336 Munich, Germany, sebastian.baumbach@med.uni-muenchen.de"],["dc.contributor.affiliation","Zustin, Jozef; \t\t \r\n\t\t Pathology, Universitätsklinikum Hamburg Eppendorf, Hamburg 20251, Germany, zustin@pathologie-hamburg.de"],["dc.contributor.affiliation","Lehmann, Wolfgang; \t\t \r\n\t\t Clinic for Trauma Surgery, Orthopaedic Surgery and Plastic Surgery, Universitätsmedizin Göttingen, 37075 Göttingen, Germany, wolfgang.lehmann@med.uni-goettingen.de"],["dc.contributor.affiliation","Schilling, Arndt F.; \t\t \r\n\t\t Clinic for Trauma Surgery, Orthopaedic Surgery and Plastic Surgery, Universitätsmedizin Göttingen, 37075 Göttingen, Germany, arndt.schilling@med.uni-goettingen.de"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Winkler, Thomas"],["dc.contributor.author","Schenk, Lia Sabrina"],["dc.contributor.author","Neuerburg, Carl"],["dc.contributor.author","Baumbach, Sebastian Felix"],["dc.contributor.author","Zustin, Jozef"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2019-07-09T11:50:07Z"],["dc.date.available","2019-07-09T11:50:07Z"],["dc.date.issued","2019"],["dc.date.updated","2022-09-05T16:05:35Z"],["dc.description.abstract","It is widely accepted that the subchondral bone (SCB) plays a crucial role in the physiopathology of osteoarthritis (OA), although its contribution is still debated. Much of the pre-clinical research on the role of SCB is concentrated on comparative evaluations of healthy vs. early OA or early OA vs. advanced OA cases, while neglecting how pure maturation could change the SCB's microstructure. To assess the transformations of the healthy SCB from young age to early adulthood, we examined the microstructure and material composition of the medial condyle of the femur in calves (three months) and cattle (18 months) for the calcified cartilage (CC) and the subchondral bone plate (SCBP). The entire subchondral zone (SCZ) was significantly thicker in cattle compared to calves, although the proportion of the CC and SCBP thicknesses were relatively constant. The trabecular number (Tb.N.) and the connectivity density (Conn.D) were significantly higher in the deeper region of the SCZ, while the bone volume fraction (BV/TV), and the degree of anisotropy (DA) were more affected by age rather than the region. The mineralization increased within the first 250 µm of the SCZ irrespective of sample type, and became stable thereafter. Cattle exhibited higher mineralization than calves at all depths, with a mean Ca/P ratio of 1.59 and 1.64 for calves and cattle, respectively. Collectively, these results indicate that the SCZ is highly dynamic at early age, and CC is the most dynamic layer of the SCZ."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/ijms20030770"],["dc.identifier.pmid","30759738"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15863"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59705"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1422-0067"],["dc.relation.issn","1422-0067"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Developmental Transformation and Reduction of Connective Cavities within the Subchondral Bone"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Yoshida, Takashi"],["dc.contributor.author","Böker, Kai O."],["dc.contributor.author","Foerster, Robert H."],["dc.contributor.author","Jochim, Lina"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt Friedrich"],["dc.date.accessioned","2021-06-01T09:42:49Z"],["dc.date.available","2021-06-01T09:42:49Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The interplay between articular cartilage (AC) and subchondral bone (SB) plays a pivotal role in cartilage homeostasis and functionality. As direct connective pathways between the two are poorly understood, we examined the location-dependent characteristics of the 3D microchannel network within the SB that connects the basal cartilage layer to the bone marrow (i.e. cartilage-bone marrow microchannel connectors; CMMC). 43 measuring points were defined on five human cadaveric femoral heads with no signs of osteoarthritis (OA) (age ≤ 60), and cartilage-bone cylinders with diameters of 2.00 mm were extracted for high-resolution scanning ( n  = 215). The micro-CT data were categorized into three groups (load-bearing region: LBR, n  = 60; non-load-bearing region: NLBR, n  = 60; and the peripheral rim: PR, n  = 95) based on a gait analysis estimation of the joint reaction force (young, healthy cohort with no signs of OA). At the AC-SB interface, the number of CMMC in the LBR was 1.8 times and 2.2 times higher compared to the NLBR, and the PR, respectively. On the other hand, the median Feret size of the CMMC were smallest in the LBR (55.2 µm) and increased in the NLBR (73.5 µm; p  = 0.043) and the PR (89.1 µm; p  = 0.043). AC thickness was positively associated with SB thickness (Pearson's r  = 0.48; p  < 1e-13), CMMC number. ( r  = 0.46; p  < 1e-11), and circularity index ( r  = 0.61; p  < 1e-38). In conclusion, our data suggest that regional differences in the microchannel architecture of SB might reflect regional differences in loading."],["dc.identifier.doi","10.1007/s00223-021-00864-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85362"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1432-0827"],["dc.relation.issn","0171-967X"],["dc.title","Investigating the Microchannel Architectures Inside the Subchondral Bone in Relation to Estimated Hip Reaction Forces on the Human Femoral Head"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9887"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Shang, Xiaobin"],["dc.contributor.author","Böker, Kai Oliver"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Hawellek, Thelonius"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2021-10-01T09:58:22Z"],["dc.date.available","2021-10-01T09:58:22Z"],["dc.date.issued","2021"],["dc.date.updated","2022-09-04T00:18:30Z"],["dc.description.abstract","Osteoarthritis (OA) is a chronic disease affecting the whole joint, which still lacks a disease-modifying treatment. This suggests an incomplete understanding of underlying molecular mechanisms. The Wnt/β-catenin pathway is involved in different pathophysiological processes of OA. Interestingly, both excessive stimulation and suppression of this pathway can contribute to the pathogenesis of OA. microRNAs have been shown to regulate different cellular processes in different diseases, including the metabolic activity of chondrocytes and osteocytes. To bridge these findings, here we attempt to give a conclusive overview of microRNA regulation of the Wnt/β-catenin pathway in bone and cartilage, which may provide insights to advance the development of miRNA-based therapeutics for OA treatment."],["dc.description.abstract","Osteoarthritis (OA) is a chronic disease affecting the whole joint, which still lacks a disease-modifying treatment. This suggests an incomplete understanding of underlying molecular mechanisms. The Wnt/β-catenin pathway is involved in different pathophysiological processes of OA. Interestingly, both excessive stimulation and suppression of this pathway can contribute to the pathogenesis of OA. microRNAs have been shown to regulate different cellular processes in different diseases, including the metabolic activity of chondrocytes and osteocytes. To bridge these findings, here we attempt to give a conclusive overview of microRNA regulation of the Wnt/β-catenin pathway in bone and cartilage, which may provide insights to advance the development of miRNA-based therapeutics for OA treatment."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/ijms22189887"],["dc.identifier.pii","ijms22189887"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90051"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","1422-0067"],["dc.relation.orgunit","Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie"],["dc.rights","CC BY 4.0"],["dc.title","The Interaction between microRNAs and the Wnt/β-Catenin Signaling Pathway in Osteoarthritis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3975"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Materials"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Böker, Kai O."],["dc.contributor.author","Richter, Katharina"],["dc.contributor.author","Jäckle, Katharina"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Grunwald, Ingo"],["dc.contributor.author","Borcherding, Kai"],["dc.contributor.author","von Byern, Janek"],["dc.contributor.author","Hartwig, Andreas"],["dc.contributor.author","Wildemann, Britt"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Lehmann, Wolfgang"],["dc.date.accessioned","2020-12-10T18:47:15Z"],["dc.date.available","2020-12-10T18:47:15Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3390/ma12233975"],["dc.identifier.eissn","1996-1944"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16940"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78693"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1996-1944"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Current State of Bone Adhesives—Necessities and Hurdles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3526"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Materials"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Böker, Kai Oliver"],["dc.contributor.author","Kleinwort, Frederick"],["dc.contributor.author","Klein-Wiele, Jan-Hendrick"],["dc.contributor.author","Simon, Peter"],["dc.contributor.author","Jäckle, Katharina"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2021-04-14T08:23:46Z"],["dc.date.available","2021-04-14T08:23:46Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/ma13163526"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81039"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1996-1944"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]