Schilling, Arndt Friedrich

[["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biomedicines"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Jiang, Jun; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.affiliation","Röper, Lynn; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.affiliation","Alageel, Sarah; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.affiliation","Dornseifer, Ulf; 2Department of Plastic, Reconstructive and Aesthetic Surgery, Isar Klinikum, D-80331 Munich, Germany; ulf.dornseifer@isarklinikum.de"],["dc.contributor.affiliation","Schilling, Arndt F.; 3Department of Trauma Surgery, Orthopedics and Plastic Surgery, Universitätsmedizin Göttingen, D-37075 Göttingen, Germany; arndt.schilling@med.uni-goettingen.de"],["dc.contributor.affiliation","Hadjipanayi, Ektoras; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.affiliation","Machens, Hans-Günther; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.affiliation","Moog, Philipp; 1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, D-81675 Munich, Germany; junqing.jiang@mri.tum.de (J.J.); lynn.roeper@mri.tum.de (L.R.); sarah.alageel@mri.tum.de (S.A.); e.hadjipanayi@googlemail.com (E.H.)"],["dc.contributor.author","Jiang, Jun"],["dc.contributor.author","Röper, Lynn"],["dc.contributor.author","Alageel, Sarah"],["dc.contributor.author","Dornseifer, Ulf"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Hadjipanayi, Ektoras"],["dc.contributor.author","Machens, Hans-Günther"],["dc.contributor.author","Moog, Philipp"],["dc.date.accessioned","2022-08-04T08:38:41Z"],["dc.date.available","2022-08-04T08:38:41Z"],["dc.date.issued","2022-07-07"],["dc.date.updated","2022-08-03T10:01:16Z"],["dc.description.abstract","Interest in discovering new methods of employing natural growth factor preparations to promote bone fracture healing is becoming increasingly popular in the field of regenerative medicine. In this study, we were able to demonstrate the osteogenic potential of hypoxia preconditioned serum (HPS) on human osteoblasts in vitro. Human osteoblasts were stimulated with two HPS concentrations (10% and 40%) and subsequently analyzed at time points of days 2 and 4. In comparison to controls, a time- and dose-dependent (up to 14.2× higher) proliferation of osteoblasts was observed after 4 days of HPS-40% stimulation with lower lactate dehydrogenase (LDH)-levels detected than controls, indicating the absence of cytotoxic/stress effects of HPS on human osteoblasts. With regards to cell migration, it was found to be significantly faster with HPS-10% application after 72 h in comparison to controls. Further osteogenic response to HPS treatment was evaluated by employing culture supernatant analysis, which exhibited significant upregulation of OPG (Osteoprotegerin) with higher dosage (HPS-10% vs. HPS-40%) and longer duration (2 d vs. 4 d) of HPS stimulation. There was no detection of anti-osteogenic sRANKL (soluble Receptor Activator of NF-κB Ligand) after 4 days of HPS stimulation. In addition, ALP (alkaline phosphatase)-enzyme activity, was found to be upregulated, dose-dependently, after 4 days of HPS-40% application. When assessing ossification through Alizarin-Red staining, HPS dose-dependently achieved greater (up to 2.8× higher) extracellular deposition of calcium-phosphate with HPS-40% in comparison to controls. These findings indicate that HPS holds the potential to accelerate bone regeneration by osteogenic promotion of human osteoblasts."],["dc.description.sponsorship","Technical University of Munich"],["dc.identifier.doi","10.3390/biomedicines10071631"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112636"],["dc.language.iso","en"],["dc.relation.eissn","2227-9059"],["dc.rights","CC BY 4.0"],["dc.title","Hypoxia Preconditioned Serum (HPS) Promotes Osteoblast Proliferation, Migration and Matrix Deposition"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","443"],["dc.bibliographiccitation.journal","Molecular Therapy - Nucleic Acids"],["dc.bibliographiccitation.lastpage","452"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Hoffmann, Daniel B."],["dc.contributor.author","Gruber, Jens"],["dc.contributor.author","Böker, Kai O."],["dc.contributor.author","Deppe, Delia"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Lemus-Diaz, Nicolas"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Schneider, Stefan"],["dc.date.accessioned","2019-07-09T11:45:51Z"],["dc.date.available","2019-07-09T11:45:51Z"],["dc.date.issued","2018"],["dc.description.abstract","Rebalancing of the RANKL/OPG system seems to be an effective treatment strategy in postmenopausal osteoporosis. Here, we evaluate the knockdown of RANKL by in-vivo-delivered siRNA in a rat model of osteoporosis. Virus-like-particles (VLPs) derived from polyoma JC virus were used for delivering RANKL siRNA in ovariectomized (OVX) rats. 48 rats were ovariectomized and treated with either 17β-estradiol (E2), VLPs containing RANKL siRNA (siRANKL), or VLPs containing non-cognate siRNA (siCtrl). All OVX groups were subdivided into the prophylaxis group (PG) and the therapy group (TG). The PG received treatment directly after being OVX for 10 weeks. The TG received treatment 5 weeks after being OVX for 5 weeks. Rats were sacrificed 10 weeks after being OVX. Bone and blood samples were analyzed. E2 and siRANKL showed a significant knockdown of RANKL mRNA. A protein knockdown was observed with E2 and siRANKL in the TG but not in the PG. No distinct improvements in biomechanical and morphological properties of the bones were observed after siRANKL treatment. In the PG, E2 protected the bone structure. We demonstrated successful mRNA and protein knockdown by VLP-mediated RNAi in vivo. Knockdown of membranous RANKL did not result in significant improvements of bone properties in this model of early-stage postmenopausal osteoporosis."],["dc.identifier.doi","10.1016/j.omtn.2018.06.001"],["dc.identifier.pmid","30195781"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15329"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59322"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2162-2531"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Effects of RANKL Knockdown by Virus-like Particle-Mediated RNAi in a Rat Model of Osteoporosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Neurorobotics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.affiliation","Mouchoux, Jérémy; 1Applied Rehabilitation Technology Lab, Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Georg-August University, Göttingen, Germany"],["dc.contributor.affiliation","Bravo-Cabrera, Miguel A.; 1Applied Rehabilitation Technology Lab, Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Georg-August University, Göttingen, Germany"],["dc.contributor.affiliation","Dosen, Strahinja; 2Faculty of Medicine, Department of Health Science and Technology Center for Sensory-Motor Interaction, Aalborg University, Aalborg, Denmark"],["dc.contributor.affiliation","Schilling, Arndt F.; 1Applied Rehabilitation Technology Lab, Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Georg-August University, Göttingen, Germany"],["dc.contributor.affiliation","Markovic, Marko; 1Applied Rehabilitation Technology Lab, Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Georg-August University, Göttingen, Germany"],["dc.contributor.author","Mouchoux, Jérémy"],["dc.contributor.author","Bravo-Cabrera, Miguel A."],["dc.contributor.author","Dosen, Strahinja"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Markovic, Marko"],["dc.date.accessioned","2022-02-01T10:31:40Z"],["dc.date.available","2022-02-01T10:31:40Z"],["dc.date.issued","2021"],["dc.date.updated","2022-02-09T13:20:13Z"],["dc.description.abstract","Semi-autonomous (SA) control of upper-limb prostheses can improve the performance and decrease the cognitive burden of a user. In this approach, a prosthesis is equipped with additional sensors (e.g., computer vision) that provide contextual information and enable the system to accomplish some tasks automatically. Autonomous control is fused with a volitional input of a user to compute the commands that are sent to the prosthesis. Although several promising prototypes demonstrating the potential of this approach have been presented, methods to integrate the two control streams (i.e., autonomous and volitional) have not been systematically investigated. In the present study, we implemented three shared control modalities (i.e., sequential, simultaneous , and continuous ) and compared their performance, as well as the cognitive and physical burdens imposed on the user. In the sequential approach, the volitional input disabled the autonomous control. In the simultaneous approach, the volitional input to a specific degree of freedom (DoF) activated autonomous control of other DoFs, whereas in the continuous approach, autonomous control was always active except for the DoFs controlled by the user. The experiment was conducted in ten able-bodied subjects, and these subjects used an SA prosthesis to perform reach-and-grasp tasks while reacting to audio cues (dual tasking). The results demonstrated that, compared to the manual baseline (volitional control only), all three SA modalities accomplished the task in a shorter time and resulted in less volitional control input. The simultaneous SA modality performed worse than the sequential and continuous SA approaches. When systematic errors were introduced in the autonomous controller to generate a mismatch between the goals of the user and controller, the performance of SA modalities substantially decreased, even below the manual baseline. The sequential SA scheme was the least impacted one in terms of errors. The present study demonstrates that a specific approach for integrating volitional and autonomous control is indeed an important factor that significantly affects the performance and physical and cognitive load, and therefore these should be considered when designing SA prostheses."],["dc.description.abstract","Semi-autonomous (SA) control of upper-limb prostheses can improve the performance and decrease the cognitive burden of a user. In this approach, a prosthesis is equipped with additional sensors (e.g., computer vision) that provide contextual information and enable the system to accomplish some tasks automatically. Autonomous control is fused with a volitional input of a user to compute the commands that are sent to the prosthesis. Although several promising prototypes demonstrating the potential of this approach have been presented, methods to integrate the two control streams (i.e., autonomous and volitional) have not been systematically investigated. In the present study, we implemented three shared control modalities (i.e., sequential, simultaneous , and continuous ) and compared their performance, as well as the cognitive and physical burdens imposed on the user. In the sequential approach, the volitional input disabled the autonomous control. In the simultaneous approach, the volitional input to a specific degree of freedom (DoF) activated autonomous control of other DoFs, whereas in the continuous approach, autonomous control was always active except for the DoFs controlled by the user. The experiment was conducted in ten able-bodied subjects, and these subjects used an SA prosthesis to perform reach-and-grasp tasks while reacting to audio cues (dual tasking). The results demonstrated that, compared to the manual baseline (volitional control only), all three SA modalities accomplished the task in a shorter time and resulted in less volitional control input. The simultaneous SA modality performed worse than the sequential and continuous SA approaches. When systematic errors were introduced in the autonomous controller to generate a mismatch between the goals of the user and controller, the performance of SA modalities substantially decreased, even below the manual baseline. The sequential SA scheme was the least impacted one in terms of errors. The present study demonstrates that a specific approach for integrating volitional and autonomous control is indeed an important factor that significantly affects the performance and physical and cognitive load, and therefore these should be considered when designing SA prostheses."],["dc.identifier.doi","10.3389/fnbot.2021.768619"],["dc.identifier.eissn","1662-5218"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98918"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1662-5218"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Impact of Shared Control Modalities on Performance and Usability of Semi-autonomous Prostheses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","365"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biomedicines"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Moog, Philipp"],["dc.contributor.author","Schams, Rahmin"],["dc.contributor.author","Schneidinger, Alexander"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Machens, Hans-Günther"],["dc.contributor.author","Hadjipanayi, Ektoras"],["dc.contributor.author","Dornseifer, Ulf"],["dc.date.accessioned","2021-04-14T08:32:36Z"],["dc.date.available","2021-04-14T08:32:36Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/biomedicines8090365"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83962"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","2227-9059"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Effect of Hypoxia Preconditioned Secretomes on Lymphangiogenic and Angiogenic Sprouting: An in Vitro Analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0174860"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Perisic, Tatjana"],["dc.contributor.author","Zhang, Z."],["dc.contributor.author","Foehr, Peter"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Klutz, Kathrin"],["dc.contributor.author","Burgkart, Rainer H."],["dc.contributor.author","Slobodianski, Alexei"],["dc.contributor.author","Goeldner, Moritz"],["dc.contributor.author","Machens, Hans-Gunther"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2018-11-07T10:25:06Z"],["dc.date.available","2018-11-07T10:25:06Z"],["dc.date.issued","2017"],["dc.description.abstract","Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine."],["dc.identifier.doi","10.1371/journal.pone.0174860"],["dc.identifier.isi","000399353500069"],["dc.identifier.pmid","28380080"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42784"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for geneticallymodified fibroblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","22"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Functional Biomaterials"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hadjipanayi, Ektoras"],["dc.contributor.author","Moog, Philipp"],["dc.contributor.author","Bekeran, Sanjar"],["dc.contributor.author","Kirchhoff, Katharina"],["dc.contributor.author","Berezhnoi, Andrei"],["dc.contributor.author","Aguirre, Juan"],["dc.contributor.author","Bauer, Anna-Theresa"],["dc.contributor.author","Kükrek, Haydar"],["dc.contributor.author","Schmauss, Daniel"],["dc.contributor.author","Hopfner, Ursula"],["dc.contributor.author","Isenburg, Sarah"],["dc.contributor.author","Ntziachristos, Vasilis"],["dc.contributor.author","Ninkovic, Milomir"],["dc.contributor.author","Machens, Hans-Günther"],["dc.contributor.author","Schilling, Arndt F."],["dc.contributor.author","Dornseifer, Ulf"],["dc.date.accessioned","2020-12-10T18:47:13Z"],["dc.date.available","2020-12-10T18:47:13Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3390/jfb10020022"],["dc.identifier.eissn","2079-4983"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78685"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2079-4983"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","In Vitro Characterization of Hypoxia Preconditioned Serum (HPS)—Fibrin Hydrogels: Basis for an Injectable Biomimetic Tissue Regeneration Therapy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1000191"],["dc.bibliographiccitation.issue","01"],["dc.bibliographiccitation.journal","Journal of Osteoporosis and Physical Activity"],["dc.bibliographiccitation.volume","05"],["dc.contributor.author","Saul, D."],["dc.contributor.author","Schilling, A. F."],["dc.contributor.author","Kosinsky, R. L."],["dc.date.accessioned","2019-07-09T11:43:26Z"],["dc.date.available","2019-07-09T11:43:26Z"],["dc.date.issued","2017"],["dc.description.abstract","In an aging population, the decline in muscle mass and strength in combination with a high prevalence of osteoporosis and cancer leads to a multitude of clinical manifestations. In the recent years, mouse models of wasting in cancer and inflammation, including xenograft, genetic and chemically induced models, allowed to uncover several key mechanisms underlying muscle loss. These include inflammation, hormone alterations and deregulated protein degradation. Inflammation is associated with increased expression of tumor necrosis factor α (TNF-α), nuclear factor κB (NF-κB), and interleukin (IL)-6 and is therefore linked to inflammatory bowel diseases or chronic obstructive pulmonary disease (COPD). Moreover, active NF-κB signaling and IL-6 secretion commonly occurs in malignancies and cancer-induced cachexia. The ubiquitin proteasome-mediated degradation of proteins represents a second pathway underlying sarcopenia and is partially initiated by inflammatory signaling. Consequently, increased levels of the E3 ligases Muscle RING-Finger Protein-1 (MuRF1), Atrogin-1/Muscle Atrophy F-box (MAFbx), and tumor necrosis factor α receptor adaptor protein 6 (TRAF6) are associated with high rates of protein degradation. Furthermore, hormonal alterations, such as the aging-related decline of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), lead to a reduction of muscle mass. Interestingly, experimental targeting of several of those sarcopenia-associated factors in vivo resulted in a rescue of muscle mass and function. While therapeutic options nowadays still need to be evaluated regarding their clinical practicability, IL-6 antibodies, inhibition of cyclooxygenases and inhibitors of myostatin appear promising."],["dc.identifier.doi","10.4172/2329-9509.1000191"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14528"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58888"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2329-9509"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Why Age Matters: Inflammation, Cancer and Hormones in the Development of Sarcopenia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","100224"],["dc.bibliographiccitation.journal","Bone Reports"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Rechholtz, C."],["dc.contributor.author","Pohlmann, N."],["dc.contributor.author","Lehmann, W."],["dc.contributor.author","Schilling, A. F."],["dc.contributor.author","Wigger, R."],["dc.contributor.author","Sehmisch, S."],["dc.contributor.author","Hoffmann, D. B."],["dc.date.accessioned","2019-09-24T08:05:04Z"],["dc.date.available","2019-09-24T08:05:04Z"],["dc.date.issued","2019"],["dc.description.abstract","Bisphosphonate alendronate (ALN), phytoestrogen 8-prenylnaringenin (8-PN) and the whole body vibration exert a favorable effect on osteoporotic bone. However, the impact of these treatments and the combination of pharmacological therapies with biomechanical stimulation on muscle and bone has not yet been explored in detail. The effect of ALN and 8-PN and their combination with the vibration (Vib) on skeletal muscle and bone healing was investigated in ovariectomized (Ovx) rats. Three-month old rats were Ovx (n = 78), or left intact (Non-Ovx; n = 12). Five weeks after Ovx, all rats were treated according to the group assignment (n = 12/13): 1) Non-Ovx; 2) Ovx; 3) Ovx + Vib; 4) Ovx + ALN; 5) Ovx + ALN + Vib; 6): Ovx + 8-PN; 7) Ovx + 8-PN + Vib. Treatments with ALN (0.58 mg/kg BW, in food), 8-PN (1.77 mg/kg BW, daily s.c. injections) and/or with vertical vibration (0.5 mm, 35 Hz, 1 g, 15 min, 2×/day, 5×/week) were conducted for ten weeks. Nine weeks after Ovx, all rats underwent bilateral tibia osteotomy with plate osteosynthesis and were sacrificed six weeks later. Vibration increased fiber size and capillary density in muscle, enlarged callus area and width, and decreased callus density in tibia, and elevated alkaline phosphatase in serum. ALN and ALN + Vib enhanced capillarization and lactate dehydrogenase activity in muscle. In tibia, ALN slowed bone healing, ALN + Vib increased callus width and density, enhanced callus formation rate and expression of osteogenic genes. 8-PN and 8-PN + Vib decreased fiber size and increased capillary density in muscle; callus density and cortical width were reduced in tibia. Vibration worsened 8-PN effect on bone healing decreasing the callus width and area. Our data suggest that Vib, ALN, 8-PN, or 8-PN + Vib do not appear to aid bone healing. ALN + Vib improved bone healing; however application is questionable since single treatments impaired bone healing. Muscle responds to the anti-osteoporosis treatments and should be included in the evaluation of the drugs."],["dc.identifier.doi","10.1016/j.bonr.2019.100224"],["dc.identifier.pmid","31516917"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16393"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62450"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2352-1872"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Effect of alendronate or 8-prenylnaringenin applied as a single therapy or in combination with vibration on muscle structure and bone healing in ovariectomized rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Bioengineering and Biotechnology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Liu, Juan"],["dc.contributor.author","Zheng, Huaiyuan"],["dc.contributor.author","Dai, Xinyi"],["dc.contributor.author","Poh, Patrina S. P."],["dc.contributor.author","Machens, Hans-Günther"],["dc.contributor.author","Schilling, Arndt F."],["dc.date.accessioned","2021-04-14T08:31:17Z"],["dc.date.available","2021-04-14T08:31:17Z"],["dc.date.issued","2020"],["dc.description.abstract","Tissue engineering in combination with stem cell technology has the potential to revolutionize human healthcare. It aims at the generation of artificial tissues that can mimic the original with complex functions for medical applications. However, even the best current designs are limited in size, if the transport of nutrients and oxygen to the cells and the removal of cellular metabolites waste is mainly dependent on passive diffusion. Incorporation of functional biomimetic vasculature within tissue engineered constructs can overcome this shortcoming. Here, we developed a novel strategy using 3D printing and injection molding technology to customize multilayer hydrogel constructs with pre-vascularized structures in transparent Polydimethysiloxane (PDMS) bioreactors. These bioreactors can be directly connected to continuous perfusion systems without complicated construct assembling. Mimicking natural layer-structures of vascular walls, multilayer vessel constructs were fabricated with cell-laden fibrin and collagen gels, respectively. The multilayer design allows functional organization of multiple cell types, i.e., mesenchymal stem cells (MSCs) in outer layer, human umbilical vein endothelial cells (HUVECs) the inner layer and smooth muscle cells in between MSCs and HUVECs layers. Multiplex layers with different cell types showed clear boundaries and growth along the hydrogel layers. This work demonstrates a rapid, cost-effective, and practical method to fabricate customized 3D-multilayer vascular models. It allows precise design of parameters like length, thickness, diameter of lumens and the whole vessel constructs resembling the natural tissue in detail without the need of sophisticated skills or equipment. The ready-to-use bioreactor with hydrogel constructs could be used for biomedical applications including pre-vascularization for transplantable engineered tissue or studies of vascular biology."],["dc.identifier.doi","10.3389/fbioe.2020.568934"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83544"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","2296-4185"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Transparent PDMS Bioreactors for the Fabrication and Analysis of Multi-Layer Pre-vascularized Hydrogels Under Continuous Perfusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","129"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","International Journal of Environmental Research and Public Health"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Assmann, Mara"],["dc.contributor.author","Steinmetz, Gino"],["dc.contributor.author","Schilling, Arndt Friedrich"],["dc.contributor.author","Saul, Dominik"],["dc.date.accessioned","2021-04-14T08:29:43Z"],["dc.date.available","2021-04-14T08:29:43Z"],["dc.date.issued","2020-12-27"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/ijerph18010129"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17796"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82971"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1660-4601"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Comparison of Grip Strength in Recreational Climbers and Non-Climbing Athletes—A Cross-Sectional Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]