Claussen, Maike

[["dc.bibliographiccitation.firstpage","214"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","224"],["dc.bibliographiccitation.volume","405"],["dc.contributor.author","Bauermeister, Diana"],["dc.contributor.author","Claussen, Maike"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T09:51:39Z"],["dc.date.available","2018-11-07T09:51:39Z"],["dc.date.issued","2015"],["dc.description.abstract","The localization of certain mRNAs to the vegetal cortex of Xenopus oocytes is of crucial importance for germ cell development and early embryonic patterning. Vegetal RNA localization is mediated by cis-acting RNA localization elements (LE). Several proteins assemble on the RNA LE and direct transport to the vegetal cortex. Although a number of localization RNP components have been identified, their full composition is unknown. In an RNA affinity purification approach, using the dead end 1 (dnd1) RNA LE, we identified Xenopus Celf1 as a novel component of vegetal localization RNP complexes. Celf1 is part of an RNP complex together with known vegetal localization factors and shows specific interactions with LEs from several but not all vegetally localizing RNAs. Immunostaining experiments reveal co-localization of Celf1 with vegetally localizing RNA and with known localization factors. Inhibition of Celf1 protein binding by localization element mutagenesis as well as Celf1 overexpression interfere with vegetal RNA localization. These results argue for a role of Celf1 in vegetal RNA localization during Xeno pus oogenesis. (C) 2015 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.ydbio.2015.07.005"],["dc.identifier.isi","000360255800004"],["dc.identifier.pmid","26164657"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35956"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","1095-564X"],["dc.relation.issn","0012-1606"],["dc.title","A novel role for Celf1 in vegetal RNA localization during Xenopus oogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","425"],["dc.bibliographiccitation.issue","10-12"],["dc.bibliographiccitation.journal","The International Journal of Developmental Biology"],["dc.bibliographiccitation.lastpage","433"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Bauermeister, Diana"],["dc.contributor.author","Claussen, Maike"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T10:02:28Z"],["dc.date.available","2018-11-07T10:02:28Z"],["dc.date.issued","2015"],["dc.description.abstract","In Xenopus laevis, maternal transcripts that localize to the vegetal cortex of the oocyte are specifically inherited by prospective germ cells during cleavage stages. While a large fraction of maternal transcripts is degraded during the maternal to zygotic transition (MZT), transcripts associated with the germ-line are stable. A sequence in the dead end 1 3'UTR mediates vegetal localization in the oocyte as well as miR mediated clearance in somatic cells and germ cell specific stabilization during the MZT in embryos. We could identify Tia1 to co-precipitate with known components of vegetal localization RNPs in X. laevis oocytes. Tia1 interacts and co-localizes with various localization elements from vegetally localizing RNAs. In X. laevis embryos, ectopic expression of Tia1 counteracts somatic degradation of dnd1 localization element reporter RNAs and it can synergize with Dnd1 protein in reporter RNA stabilization. Ectopic Tia1 also protects several endogenous localizing and germ cell specific mRNAs from somatic degradation. Thus, proteins that protect germ-line transcripts from miR mediated decay during the MZT in embryos might bind these RNAs already in the oocyte."],["dc.identifier.doi","10.1387/ijdb.150137db"],["dc.identifier.isi","000372858700002"],["dc.identifier.pmid","26864483"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38231"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Univ Basque Country Upv-ehu Press"],["dc.relation.issn","1696-3547"],["dc.relation.issn","0214-6282"],["dc.title","T-cell internal antigen 1 counteracts somatic RNA degradation during early Xenopus embryogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]