Fischer, Andreas

[["dc.bibliographiccitation.firstpage","3086"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","11"],["dc.contributor.affiliation","Jarausch, Johannes; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Neuenroth, Lisa; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Andag, Reiner; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Leha, Andreas; 3Department of Medical Statistics, University Medical Center Goettingen, Humboldtallee 32, 37073 Goettingen, Germany"],["dc.contributor.affiliation","Fischer, Andreas; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Asif, Abdul R.; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Lenz, Christof; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.affiliation","Eidizadeh, Abass; 1Department of Clinical Chemistry and Interdisciplinary UMG Laboratory, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075 Goettingen, Germany"],["dc.contributor.author","Jarausch, Johannes"],["dc.contributor.author","Neuenroth, Lisa"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Leha, Andreas"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Asif, Abdul R."],["dc.contributor.author","Lenz, Christof"],["dc.contributor.author","Eidizadeh, Abass"],["dc.contributor.editor","Zhou, Changcheng"],["dc.contributor.editor","Chen, Hong"],["dc.date.accessioned","2022-11-01T10:17:24Z"],["dc.date.available","2022-11-01T10:17:24Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:12:33Z"],["dc.description.abstract","Atherosclerosis is an important risk factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger atherosclerosis. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their epigenetic mechanism and anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi treatment on human endothelial cells was investigated using global protein expression profiling by ion mobility separation-enhanced data independent acquisition mass spectrometry (IMS-DIA-MS). For this purpose, primary human umbilical cord derived vascular endothelial cells were treated with TNFα to mimic inflammation and exposed to laminar shear stress in the presence or absence of the BRD4 inhibitor JQ1. IMS-DIA-MS detected over 4037 proteins expressed in endothelial cells. Inflammation, shear stress and BETi led to pronounced changes in protein expression patterns with JQ1 having the greatest effect. To our knowledge, this is the first proteomics study on primary endothelial cells, which provides an extensive database for the effects of shear stress, inflammation and BETi on the endothelial proteome."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3390/cells11193086"],["dc.identifier.pii","cells11193086"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116800"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4409"],["dc.rights","CC BY 4.0"],["dc.title","Influence of Shear Stress, Inflammation and BRD4 Inhibition on Human Endothelial Cells: A Holistic Proteomic Approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","0"],["dc.bibliographiccitation.journal","Clinical Chemistry and Laboratory Medicine (CCLM)"],["dc.bibliographiccitation.volume","0"],["dc.contributor.author","Dierks, Sascha"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Gauss, Friederike"],["dc.contributor.author","Budde, Kathrin"],["dc.contributor.author","Francke, Paul"],["dc.contributor.author","Peschka, Manuela"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Schanz, Julie"],["dc.contributor.author","Petersmann, Astrid"],["dc.date.accessioned","2022-05-02T08:09:29Z"],["dc.date.available","2022-05-02T08:09:29Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Objectives Thyroid-stimulating hormone (TSH) is the routine primary screening test to assess thyroid function and rapid measurement of TSH levels is highly desirable especially in emergency situations. In the present study, we compared the analytical performance of a commercially available point-of-care test (AFIAS-1) and five laboratory-based systems. Methods Left over material of 60 patient plasma samples was collected from patient care and used in the respective assay. For statistical analysis of the produced data Bland-Altman and Passing-Bablok regression analysis were applied. Results Good correlation (r=0.982 or higher) was found between all devices. Slopes from regression analysis ranged from 0.972 (95% CI: 0.927–1.013) to 1.276 (95% CI: 1.210–1.315). Among the compared devices, imprecision was high in terms of coefficient of variation (CV=10.3%) for low TSH concentrations and lower (CV=7.3%) for high TSH concentrations. Independent of the method used, we demonstrated a poor standardization of TSH assays, which might impact clinical diagnosis e.g. of hyperthyreosis. Conclusions This study shows that the point-of-care (POC) test AFIAS-1 can serve as an alternative to laboratory-based assays. In addition the data imply that better standardization of TSH measurements is needed."],["dc.description.abstract","Abstract Objectives Thyroid-stimulating hormone (TSH) is the routine primary screening test to assess thyroid function and rapid measurement of TSH levels is highly desirable especially in emergency situations. In the present study, we compared the analytical performance of a commercially available point-of-care test (AFIAS-1) and five laboratory-based systems. Methods Left over material of 60 patient plasma samples was collected from patient care and used in the respective assay. For statistical analysis of the produced data Bland-Altman and Passing-Bablok regression analysis were applied. Results Good correlation (r=0.982 or higher) was found between all devices. Slopes from regression analysis ranged from 0.972 (95% CI: 0.927–1.013) to 1.276 (95% CI: 1.210–1.315). Among the compared devices, imprecision was high in terms of coefficient of variation (CV=10.3%) for low TSH concentrations and lower (CV=7.3%) for high TSH concentrations. Independent of the method used, we demonstrated a poor standardization of TSH assays, which might impact clinical diagnosis e.g. of hyperthyreosis. Conclusions This study shows that the point-of-care (POC) test AFIAS-1 can serve as an alternative to laboratory-based assays. In addition the data imply that better standardization of TSH measurements is needed."],["dc.identifier.doi","10.1515/cclm-2022-0054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107390"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.eissn","1437-4331"],["dc.relation.issn","1434-6621"],["dc.title","Evaluation of the AFIAS-1 thyroid-stimulating hormone point of care test and comparison with laboratory-based devices"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]