Fischer, Andreas

[["dc.bibliographiccitation.issue","0"],["dc.bibliographiccitation.journal","Clinical Chemistry and Laboratory Medicine (CCLM)"],["dc.bibliographiccitation.volume","0"],["dc.contributor.author","Dierks, Sascha"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Gauss, Friederike"],["dc.contributor.author","Budde, Kathrin"],["dc.contributor.author","Francke, Paul"],["dc.contributor.author","Peschka, Manuela"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Schanz, Julie"],["dc.contributor.author","Petersmann, Astrid"],["dc.date.accessioned","2022-05-02T08:09:29Z"],["dc.date.available","2022-05-02T08:09:29Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Objectives Thyroid-stimulating hormone (TSH) is the routine primary screening test to assess thyroid function and rapid measurement of TSH levels is highly desirable especially in emergency situations. In the present study, we compared the analytical performance of a commercially available point-of-care test (AFIAS-1) and five laboratory-based systems. Methods Left over material of 60 patient plasma samples was collected from patient care and used in the respective assay. For statistical analysis of the produced data Bland-Altman and Passing-Bablok regression analysis were applied. Results Good correlation (r=0.982 or higher) was found between all devices. Slopes from regression analysis ranged from 0.972 (95% CI: 0.927–1.013) to 1.276 (95% CI: 1.210–1.315). Among the compared devices, imprecision was high in terms of coefficient of variation (CV=10.3%) for low TSH concentrations and lower (CV=7.3%) for high TSH concentrations. Independent of the method used, we demonstrated a poor standardization of TSH assays, which might impact clinical diagnosis e.g. of hyperthyreosis. Conclusions This study shows that the point-of-care (POC) test AFIAS-1 can serve as an alternative to laboratory-based assays. In addition the data imply that better standardization of TSH measurements is needed."],["dc.description.abstract","Abstract Objectives Thyroid-stimulating hormone (TSH) is the routine primary screening test to assess thyroid function and rapid measurement of TSH levels is highly desirable especially in emergency situations. In the present study, we compared the analytical performance of a commercially available point-of-care test (AFIAS-1) and five laboratory-based systems. Methods Left over material of 60 patient plasma samples was collected from patient care and used in the respective assay. For statistical analysis of the produced data Bland-Altman and Passing-Bablok regression analysis were applied. Results Good correlation (r=0.982 or higher) was found between all devices. Slopes from regression analysis ranged from 0.972 (95% CI: 0.927–1.013) to 1.276 (95% CI: 1.210–1.315). Among the compared devices, imprecision was high in terms of coefficient of variation (CV=10.3%) for low TSH concentrations and lower (CV=7.3%) for high TSH concentrations. Independent of the method used, we demonstrated a poor standardization of TSH assays, which might impact clinical diagnosis e.g. of hyperthyreosis. Conclusions This study shows that the point-of-care (POC) test AFIAS-1 can serve as an alternative to laboratory-based assays. In addition the data imply that better standardization of TSH measurements is needed."],["dc.identifier.doi","10.1515/cclm-2022-0054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107390"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.eissn","1437-4331"],["dc.relation.issn","1434-6621"],["dc.title","Evaluation of the AFIAS-1 thyroid-stimulating hormone point of care test and comparison with laboratory-based devices"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Li, Aijun"],["dc.contributor.author","Muenst, Simone"],["dc.contributor.author","Hoffman, Julius"],["dc.contributor.author","Starck, Laurent"],["dc.contributor.author","Sarem, Melika"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Hutter, Gregor"],["dc.contributor.author","Shastri, V. Prasad"],["dc.date.accessioned","2022-12-01T08:30:58Z"],["dc.date.available","2022-12-01T08:30:58Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n Interplay between non-cancerous cells (immune, fibroblasts, mesenchymal stromal cells (MSC), and endothelial cells (EC)) has been identified as vital in driving tumor progression. As studying such interactions in vivo is challenging, ex vivo systems that can recapitulate in vivo scenarios can aid in unraveling the factors impacting tumorigenesis and metastasis. Using the synthetic tumor microenvironment mimics (STEMs)—a spheroid system composed of breast cancer cells (BCC) with defined human MSC and EC fractions, here we show that EC organization into vascular structures is BC phenotype dependent, and independent of ERα expression in epithelial cancer cells, and involves MSC-mediated Notch1 signaling. In a 3D-bioprinted model system to mimic local invasion, MDA STEMs collectively respond to serum gradient and form invading cell clusters. STEMs grown on chick chorioallantoic membrane undergo local invasion to form CAM tumors that can anastomose with host vasculature and bear the typical hallmarks of human BC and this process requires both EC and MSC. This study provides a framework for developing well-defined in vitro systems, including patient-derived xenografts that recapitulate in vivo events, to investigate heterotypic cell interactions in tumors, to identify factors promoting tumor metastasis-related events, and possibly drug screening in the context of personalized medicine."],["dc.identifier.doi","10.1038/s42003-022-04236-5"],["dc.identifier.pii","4236"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118033"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","2399-3642"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Mesenchymal-endothelial nexus in breast cancer spheroids induces vasculogenesis and local invasion in a CAM model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","a041166"],["dc.bibliographiccitation.journal","Cold Spring Harbor Perspectives in Medicine"],["dc.contributor.author","Hasan, Sana S."],["dc.contributor.author","Fischer, Andreas"],["dc.date.accessioned","2022-07-01T07:35:05Z"],["dc.date.available","2022-07-01T07:35:05Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1101/cshperspect.a041166"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112083"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation.eissn","2157-1422"],["dc.title","Notch Signaling in the Vasculature: Angiogenesis and Angiocrine Functions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","145"],["dc.bibliographiccitation.journal","Clinica Chimica Acta"],["dc.bibliographiccitation.lastpage","151"],["dc.bibliographiccitation.volume","531"],["dc.contributor.author","Zechmeister, Bozena"],["dc.contributor.author","Erden, Tanja"],["dc.contributor.author","Kreutzig, Berit"],["dc.contributor.author","Weber, Matthias"],["dc.contributor.author","Joly, Philippe"],["dc.contributor.author","Erdmann, Jürgen"],["dc.contributor.author","Brockmann-Hönig, Christine"],["dc.contributor.author","Fischer, Andreas"],["dc.contributor.author","Eidizadeh, Abass"],["dc.date.accessioned","2022-05-02T08:09:49Z"],["dc.date.available","2022-05-02T08:09:49Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1016/j.cca.2022.03.028"],["dc.identifier.pii","S000989812200119X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107475"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.issn","0009-8981"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Analytical interference of 33 different hemoglobin variants on HbA1c measurements comparing high-performance liquid chromatography with whole blood enzymatic assay: A multi-center study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","EMBO Molecular Medicine"],["dc.contributor.author","Weis, Eva‐Maria"],["dc.contributor.author","Puchalska, Patrycja"],["dc.contributor.author","Nelson, Alisa B"],["dc.contributor.author","Taylor, Jacqueline"],["dc.contributor.author","Moll, Iris"],["dc.contributor.author","Hasan, Sana S"],["dc.contributor.author","Dewenter, Matthias"],["dc.contributor.author","Hagenmüller, Marco"],["dc.contributor.author","Fleming, Thomas"],["dc.contributor.author","Poschet, Gernot"],["dc.contributor.author","Fischer, Andreas"],["dc.date.accessioned","2022-04-01T10:01:43Z"],["dc.date.available","2022-04-01T10:01:43Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.15252/emmm.202114753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105734"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1757-4684"],["dc.relation.issn","1757-4676"],["dc.title","Ketone body oxidation increases cardiac endothelial cell proliferation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]