Dullin, Christian

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Svetlove, Angelika"],["dc.contributor.author","Zschüntzsch, Jana"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2022-09-01T09:50:08Z"],["dc.date.available","2022-09-01T09:50:08Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n \n Retrospective gating (RG) is a well established technique in preclinical computed tomography (CT) to assess 3D morphology of the lung. In RG additional angular projections are recorded typically by performing multiple rotations. Consequently, the projections are sorted according to the expansion state of the chest and those sets are then reconstructed separately. Thus, the breathing motion artefacts are suppressed at a cost of strongly elevated X-ray dose levels. Here we propose to use the entire raw data to assess respiratory motion in addition to retrospectively gated 3D reconstruction that visualize anatomical structures of the lung. Using this RG based X-ray respiratory motion measurement approach, which will be referred to as RG based X-ray lung function measurement (rgXLF) on the example of the\n mdx\n mouse model of Duchenne muscle dystrophy (mdx) we accurately obtained both the 3D anatomical morphology of the lung and the thoracic bones as well as functional temporal parameters of the lung. Thus, rgXLF will remove the necessity for separate acquisition procedures by being able to reproduce comparable results to the previously established planar X-ray based lung function measurement approach in a single low dose CT scan."],["dc.identifier.doi","10.1038/s41598-022-17335-4"],["dc.identifier.pii","17335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113630"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","2045-2322"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Simultaneous assessment of lung morphology and respiratory motion in retrospectively gated in-vivo microCT of free breathing anesthetized mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","365401"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Journal of Physics D Applied Physics"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Gureyev, Timur"],["dc.contributor.author","Mayo, S. C."],["dc.contributor.author","Nesterets, Ya I."],["dc.contributor.author","Mohammadi, S."],["dc.contributor.author","Lockie, D."],["dc.contributor.author","Menk, R. H."],["dc.contributor.author","Arfelli, F."],["dc.contributor.author","Pavlov, K. M."],["dc.contributor.author","Kitchen, M. J."],["dc.contributor.author","Zanconati, F."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Tromba, Giuliana"],["dc.date.accessioned","2018-11-07T09:35:19Z"],["dc.date.available","2018-11-07T09:35:19Z"],["dc.date.issued","2014"],["dc.description.abstract","We report the results of a systematic study of phase-contrast x-ray computed tomography in the propagation-based and analyser-based modes using specially designed phantoms and excised breast tissue samples. The study is aimed at the quantitative evaluation and subsequent optimization, with respect to detection of small tumours in breast tissue, of the effects of phase contrast and phase retrieval on key imaging parameters, such as spatial resolution, contrast-to-noise ratio, x-ray dose and a recently proposed 'intrinsic quality' characteristic which combines the image noise with the spatial resolution. We demonstrate that some of the methods evaluated in this work lead to substantial (more than 20-fold) improvement in the contrast-to-noise and intrinsic quality of the reconstructed tomographic images compared with conventional techniques, with the measured characteristics being in good agreement with the corresponding theoretical estimations. This improvement also corresponds to an approximately 400-fold reduction in the x-ray dose, compared with conventional absorption-based tomography, without a loss in the imaging quality. The results of this study confirm and quantify the significant potential benefits achievable in three-dimensional mammography using x-ray phase-contrast imaging and phase-retrieval techniques."],["dc.identifier.doi","10.1088/0022-3727/47/36/365401"],["dc.identifier.isi","000341769900016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32359"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","1361-6463"],["dc.relation.issn","0022-3727"],["dc.title","Investigation of the imaging quality of synchrotron-based phase-contrast mammographic tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","521"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","528"],["dc.bibliographiccitation.volume","90"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Klingebiel, Theda-Maria"],["dc.contributor.author","Grau, Simon Philipp"],["dc.contributor.author","Toischer, Karl"],["dc.contributor.author","Didie, Michael"],["dc.contributor.author","Jacobshagen, Claudius"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Seidler, Tim"],["dc.date.accessioned","2017-09-07T11:44:13Z"],["dc.date.available","2017-09-07T11:44:13Z"],["dc.date.issued","2011"],["dc.description.abstract","Aims The calcineurin and nuclear factor of activated T cells (NFAT) pathway can mediate pro-hypertrophic signalling in the heart. Recently, it has been shown that dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) phosphorylates NFAT, which limits calcineurin/NFAT signal transduction in T cells and hypertrophy in cultured cardiomyocytes. The hypothesis tested in this study was that DYRK1A prevents calcineurin/NFAT-mediated cardiac hypertrophy in vivo. Methods and results In cultured rat cardiomyocytes, adenovirus-mediated overexpression of DYRK1A antagonized calcineurin-mediated nuclear NFAT translocation and the phenylephrine-induced hypertrophic growth response. To test the ability of DYRK1A to reduce hypertrophic cardiac growth in vivo, we created tetracycline-repressible Dyrk1a transgenic mice to avoid the cardiac developmental defects associated with embryonic DYRK1A expression. However, in the mouse model, histological determination of myocyte diameter, heart weight/body weight ratio, and echocardiographic measurements revealed that myocardial expression of DYRK1A failed to reduce hypertrophy induced via aortic banding or co-expression of calcineurin. This discrepancy is explained, at least in part, by insufficient long-term inhibition of NFAT and the activation of DYRK1A-resistant maladaptive genes in vivo. Conclusion Isolated augmentation of DYRK1A can be compensated for in vivo, and this may significantly limit anti-hypertrophic interventions aimed at enhancing DYRK1A activity."],["dc.identifier.doi","10.1093/cvr/cvr023"],["dc.identifier.gro","3142722"],["dc.identifier.isi","000290820200018"],["dc.identifier.pmid","21273244"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/157"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0008-6363"],["dc.title","Enhanced expression of DYRK1A in cardiomyocytes inhibits acute NFAT activation but does not prevent hypertrophy in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","716"],["dc.bibliographiccitation.issue","981"],["dc.bibliographiccitation.journal","British Journal of Radiology"],["dc.bibliographiccitation.lastpage","723"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Marten, Katharina"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Machann, W."],["dc.contributor.author","Schmid, J. S."],["dc.contributor.author","Das, M."],["dc.contributor.author","Hermann, K.-P."],["dc.contributor.author","Engelke, C."],["dc.date.accessioned","2018-11-07T11:24:35Z"],["dc.date.available","2018-11-07T11:24:35Z"],["dc.date.issued","2009"],["dc.description.abstract","This study evaluates the accuracy and reproducibility of an experimental flat-panel-detector-based CT scanner (fp-CT) in comparison with those of a 64-slice multidetector row CT (MDCT) in automated pulmonary nodule volumetry. An anthropomorphic chest phantom with 31 spherical nodules (nodule diameters of 2.94-10.01 mm; volumes of 13.24-524.97 mm(3)) was scanned both with an amorphous silicon-based fp-CT scanner, using various tube current and kilovoltage settings, and with a conventional MDCT scanner. Automated nodule volumetry was performed using dedicated software. CT image data were evaluated twice by two independent radiologists. Intra-and inter-observer variations of volumetric measurements were determined and tested using the Kruskal-Wallis test and analysis of variance (fn-ANOVA). The percentage measurement errors (PME) were calculated and differences tested using Wilcoxon signed ranks and Friedman tests. Intraobserver variation was significantly higher for MDCT than for fp-CT (range: p=0.043-0.045). The measured nodule volumes were significantly greater on fp-CT than on MDCT scans (p<0.001). The PME was significantly greater in fp-CT than in MDCT scans (PME range, 12.35-13.35% for fp-CT scan protocols and 16.87-19.02% for MDCT scan protocols; p<0.0001). The PME increased significantly with reduction of nodule size, and this increase was significantly higher on MDCT than on fp-CT scans (p=0.0001). The absolute PME was significantly different for nodules of less than 5 mm in diameter (p=0.0001-0.0033) than for larger nodules. Flat-panel-detector-based CT has advantages over MDCT in accurately determining the volume of pulmonary nodules below 5 mm in diameter."],["dc.identifier.doi","10.1259/bjr/40733553"],["dc.identifier.isi","000271037700003"],["dc.identifier.pmid","19332516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56439"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","British Inst Radiology"],["dc.relation.issn","0007-1285"],["dc.title","Comparison of flat-panel-detector-based CT and multidetector-row CT in automated volumetry of pulmonary nodules using an anthropomorphic chest phantom"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2315"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Physics in Medicine and Biology"],["dc.bibliographiccitation.lastpage","2332"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Baran, P."],["dc.contributor.author","Pacile, S."],["dc.contributor.author","Nesterets, Y. I."],["dc.contributor.author","Mayo, S. C."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Dreossi, D."],["dc.contributor.author","Arfelli, F."],["dc.contributor.author","Thompson, David N."],["dc.contributor.author","Lockie, D."],["dc.contributor.author","McCormack, M. Luke"],["dc.contributor.author","Taba, S. T."],["dc.contributor.author","Brun, F."],["dc.contributor.author","Pinamonti, M."],["dc.contributor.author","Nickson, C."],["dc.contributor.author","Hall, C."],["dc.contributor.author","Dimmock, M."],["dc.contributor.author","Zanconati, F."],["dc.contributor.author","Cholewa, M."],["dc.contributor.author","Quiney, H."],["dc.contributor.author","Brennan, P. C."],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Gureyev, Timur"],["dc.date.accessioned","2018-11-07T10:26:07Z"],["dc.date.available","2018-11-07T10:26:07Z"],["dc.date.issued","2017"],["dc.description.abstract","The aim of this study was to optimise the experimental protocol and data analysis for in-vivo breast cancer x-ray imaging. Results are presented of the experiment at the SYRMEP beamline of Elettra Synchrotron using the propagation-based phase-contrast mammographic tomography method, which incorporates not only absorption, but also x-ray phase information. In this study the images of breast tissue samples, of a size corresponding to a full human breast, with radiologically acceptable x-ray doses were obtained, and the degree of improvement of the image quality (from the diagnostic point of view) achievable using propagation-based phase-contrast image acquisition protocols with proper incorporation of x-ray phase retrieval into the reconstruction pipeline was investigated. Parameters such as the x-ray energy, sample-to-detector distance and data processing methods were tested, evaluated and optimized with respect to the estimated diagnostic value using a mastectomy sample with a malignant lesion. The results of quantitative evaluation of images were obtained by means of radiological assessment carried out by 13 experienced specialists. A comparative analysis was performed between the x-ray and the histological images of the specimen. The results of the analysis indicate that, within the investigated range of parameters, both the objective image quality characteristics and the subjective radiological scores of propagation-based phase-contrast images of breast tissues monotonically increase with the strength of phase contrast which in turn is directly proportional to the product of the radiation wavelength and the sample-to-detector distance. The outcomes of this study serve to define the practical imaging conditions and the CT reconstruction procedures appropriate for low-dose phase-contrast mammographic imaging of live patients at specially designed synchrotron beamlines."],["dc.identifier.doi","10.1088/1361-6560/aa5d3d"],["dc.identifier.isi","000395886000004"],["dc.identifier.pmid","28140377"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42972"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","1361-6560"],["dc.relation.issn","0031-9155"],["dc.title","Optimization of propagation-based x-ray phase-contrast tomography for breast cancer imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","187"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.lastpage","194"],["dc.bibliographiccitation.volume","64"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schaefer, Nadine"],["dc.contributor.author","Hallecker, Jan"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T09:38:39Z"],["dc.date.available","2018-11-07T09:38:39Z"],["dc.date.issued","2014"],["dc.description.abstract","Current osteoporosis therapies aim to delay bone destruction and have additional anabolic effects. While they have demonstrated some positive effects on bone healing, more progress is needed in this area. This study used the well-known osteoporotic agents estrogen (E) and raloxifene (R) in conjunction with biomechanical whole body vibration (WBV) at a frequency of 70 Hz twice daily for six weeks to stimulate bone healing. Eighty-four 3-month old female Sprague-Dawley rats (12 per group) were bilaterally ovariectomized to develop osteopenia within eight weeks. Osteotomy of the metaphyseal tibiae was performed and fracture healing was then studied using mechanical tests, histomorphometry, computed tomography (mu CT), and gene analysis. We found that E and R improved the structure of osteopenic bones as did WBV alone, although significant levels for WBV were seldom reached. Combination treatments significantly enhanced stiffness (R + WBV; p < 0.05), endosteal bone (R + WBV; p < 0.01), and trabecular density (E + WBV; p < 0.05, R + WBV; p < 0.05). In addition, the expression of osteoclast-specific Trap was significantly reduced after treatment with E, R, or their combination with WBV (p < 0.01). The effects were additive and not inhibitory, leading us to conclude that the combined applications of WBV with E or R may improve the healing of osteopenic bones. The therapies studied are all currently approved for human use, suggesting ready applicability to clinical practice. To better understand the effects of WBV on osteopenic bones, the ideal vibration regime will require further study. (C) 2014 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","German Research Foundation (DFG) [STU 478/3-1]"],["dc.identifier.doi","10.1016/j.bone.2014.04.008"],["dc.identifier.isi","337011500026"],["dc.identifier.pmid","24735975"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33112"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1873-2763"],["dc.relation.issn","8756-3282"],["dc.title","Whole body vibration during fracture healing intensifies the effects of estradiol and raloxifene in estrogen-deficient rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6367"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Theranostics"],["dc.bibliographiccitation.lastpage","6383"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Markus, M. Andrea"],["dc.contributor.author","Heck, Joachim G."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Gorpas, Dimitris"],["dc.contributor.author","Feldmann, Claus"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-09T11:49:44Z"],["dc.date.available","2019-07-09T11:49:44Z"],["dc.date.issued","2018"],["dc.description.abstract","Treatment of inflammatory disorders with glucocorticoids (GCs) is often accompanied by severe adverse effects. Application of GCs via nanoparticles (NPs), especially those using simple formulations, could possibly improve their delivery to sites of inflammation and therefore their efficacy, minimising the required dose and thus reducing side effects. Here, we present the evaluation of NPs composed of GC betamethasone phosphate (BMP) and the fluorescent dye DY-647 (BMP-IOH-NPs) for improved treatment of inflammation with simultaneous in vivo monitoring of NP delivery. Methods: BMP-IOH-NP uptake by MH-S macrophages was analysed by fluorescence and electron microscopy. Lipopolysaccharide (LPS)-stimulated cells were treated for 48 h with BMP-IOH-NPs (1×10-5-1×10-9 M), BMP or dexamethasone (Dexa). Drug efficacy was assessed by measurement of interleukin 6. Mice with Zymosan-A-induced paw inflammation were intraperitoneally treated with BMP-IOH-NPs (10 mg/kg) and mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI) were treated intranasally with BMP-IOH-NPs, BMP or Dexa (each 2.5 mg/kg). Efficacy was assessed in vivo by paw volume measurements with µCT and ex vivo by measurement of paw weight for Zymosan-A-treated mice, or in the AAI model by in vivo x-ray-based lung function assessment and by cell counts in the bronchoalveolar lavage (BAL) fluid and histology. Delivery of BMP-IOH-NPs to the lungs of AAI mice was monitored by in vivo optical imaging and by fluorescence microscopy. Results: Uptake of BMP-IOH-NPs by MH-S cells was observed during the first 10 min of incubation, with the NP load increasing over time. The anti-inflammatory effect of BMP-IOH-NPs in vitro was dose dependent and higher than that of Dexa or free BMP, confirming efficient release of the drug. In vivo, Zymosan-A-induced paw inflammation was significantly reduced in mice treated with BMP-IOH-NPs. AAI mice that received BMP-IOH-NPs or Dexa but not BMP revealed significantly decreased eosinophil numbers in BALs and reduced immune cell infiltration in lungs. Correspondingly, lung function parameters, which were strongly affected in non-treated AAI mice, were unaffected in AAI mice treated with BMP-IOH-NPs and resembled those of healthy animals. Accumulation of BMP-IOH-NPs within the lungs of AAI mice was detectable by optical imaging for at least 4 h in vivo, where they were preferentially taken up by peribronchial and alveolar M2 macrophages. Conclusion: Our results show that BMP-IOH-NPs can effectively be applied in therapy of inflammatory diseases with at least equal efficacy as the gold standard Dexa, while their delivery can be simultaneously tracked in vivo by fluorescence imaging. BMP-IOH-NPs thus have the potential to reach clinical applications."],["dc.identifier.doi","10.7150/thno.28324"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59620"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1838-7640"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","Therapeutic Fluorescent Hybrid Nanoparticles for Traceable Delivery of Glucocorticoids to Inflammatory Sites"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e79"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Academic Radiology"],["dc.bibliographiccitation.lastpage","e89"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Tavakoli Taba, Seyedamir"],["dc.contributor.author","Baran, Patrycja"],["dc.contributor.author","Lewis, Sarah"],["dc.contributor.author","Heard, Robert"],["dc.contributor.author","Pacile, Serena"],["dc.contributor.author","Nesterets, Yakov I."],["dc.contributor.author","Mayo, Sherry C."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Dreossi, Diego"],["dc.contributor.author","Brennan, Patrick C"],["dc.date.accessioned","2021-06-01T10:49:23Z"],["dc.date.available","2021-06-01T10:49:23Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.acra.2018.07.008"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86272"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","1076-6332"],["dc.title","Toward Improving Breast Cancer Imaging: Radiological Assessment of Propagation-Based Phase-Contrast CT Technology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","874"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Cellular and Molecular Medicine"],["dc.bibliographiccitation.lastpage","887"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Stangl, Stefan"],["dc.contributor.author","Gehrmann, Mathias"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Themelis, George"],["dc.contributor.author","Ntziachristos, Vasilis"],["dc.contributor.author","Staeblein, Eva"],["dc.contributor.author","Walch, Axel"],["dc.contributor.author","Winkelmann, Isabel"],["dc.contributor.author","Multhoff, Gabriele"],["dc.date.accessioned","2018-11-07T08:57:12Z"],["dc.date.available","2018-11-07T08:57:12Z"],["dc.date.issued","2011"],["dc.description.abstract","The major stress-inducible heat shock protein 70 (Hsp70) is frequently present on the cell surface of human tumours, but not on normal cells. Herein, the binding characteristics of the cmHsp70.1 mouse monoclonal antibody (mAb) were evaluated in vitro and in a syngeneic tumour mouse model. More than 50% of the CT26 mouse colon carcinoma cells express Hsp70 on their cell surface at 4 degrees C. After a temperature shift to 37 degrees C, the cmHsp70.1-fluorescein isothiocyanate mAb translocates into early endosomes and lysosomes. Intraoperative and near-infrared fluorescence imaging revealed an enrichment of Cy5.5-conjugated mAb cmHsp70.1, but not an identically labelled IgG1 isotype-matched control, in i.p. and s.c. located CT26 tumours, as soon as 30 min. after i.v. injection into the tail vein. Due to the rapid turnover rate of membrane-bound Hsp70, the fluorescence-labelled cmHsp70.1 mAb became endocytosed and accumulated in the tumour, reaching a maximum after 24 hrs and remained detectable at least up to 96 hrs after a single i.v. injection. The tumour-selective internalization of mAb cmHsp70.1 at the physiological temperature of 37 degrees C might enable a targeted uptake of toxins or radionuclides into Hsp70 membrane-positive tumours. The anti-tumoral activity of the cmHsp70.1 mAb is further supported by its capacity to mediate antibody-dependent cytotoxicity."],["dc.identifier.doi","10.1111/j.1582-4934.2010.01067.x"],["dc.identifier.isi","000290312700015"],["dc.identifier.pmid","20406322"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23338"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1582-1838"],["dc.title","In vivo imaging of CT26 mouse tumours by using cmHsp70.1 monoclonal antibody"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","743"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","744"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Grebe, Cornelia"],["dc.contributor.author","Klingebiel, Theda-Maria"],["dc.contributor.author","Grau, Simon Philipp"],["dc.contributor.author","Toischer, Karl"],["dc.contributor.author","Didie, Michael"],["dc.contributor.author","Jacobshagen, Claudius"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Seidler, Tim"],["dc.date.accessioned","2018-11-07T08:52:46Z"],["dc.date.available","2018-11-07T08:52:46Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1093/cvr/cvr193"],["dc.identifier.isi","000294069300024"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22252"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0008-6363"],["dc.title","Letter concerning: 'Enhanced expression of DYRK1A in cardiomyocytes inhibits acute NFAT activation but does not prevent hypertrophy in vivo': reply"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]