Dullin, Christian

[["dc.bibliographiccitation.artnumber","365401"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Journal of Physics D Applied Physics"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Gureyev, Timur"],["dc.contributor.author","Mayo, S. C."],["dc.contributor.author","Nesterets, Ya I."],["dc.contributor.author","Mohammadi, S."],["dc.contributor.author","Lockie, D."],["dc.contributor.author","Menk, R. H."],["dc.contributor.author","Arfelli, F."],["dc.contributor.author","Pavlov, K. M."],["dc.contributor.author","Kitchen, M. J."],["dc.contributor.author","Zanconati, F."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Tromba, Giuliana"],["dc.date.accessioned","2018-11-07T09:35:19Z"],["dc.date.available","2018-11-07T09:35:19Z"],["dc.date.issued","2014"],["dc.description.abstract","We report the results of a systematic study of phase-contrast x-ray computed tomography in the propagation-based and analyser-based modes using specially designed phantoms and excised breast tissue samples. The study is aimed at the quantitative evaluation and subsequent optimization, with respect to detection of small tumours in breast tissue, of the effects of phase contrast and phase retrieval on key imaging parameters, such as spatial resolution, contrast-to-noise ratio, x-ray dose and a recently proposed 'intrinsic quality' characteristic which combines the image noise with the spatial resolution. We demonstrate that some of the methods evaluated in this work lead to substantial (more than 20-fold) improvement in the contrast-to-noise and intrinsic quality of the reconstructed tomographic images compared with conventional techniques, with the measured characteristics being in good agreement with the corresponding theoretical estimations. This improvement also corresponds to an approximately 400-fold reduction in the x-ray dose, compared with conventional absorption-based tomography, without a loss in the imaging quality. The results of this study confirm and quantify the significant potential benefits achievable in three-dimensional mammography using x-ray phase-contrast imaging and phase-retrieval techniques."],["dc.identifier.doi","10.1088/0022-3727/47/36/365401"],["dc.identifier.isi","000341769900016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32359"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","1361-6463"],["dc.relation.issn","0022-3727"],["dc.title","Investigation of the imaging quality of synchrotron-based phase-contrast mammographic tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2315"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Physics in Medicine and Biology"],["dc.bibliographiccitation.lastpage","2332"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Baran, P."],["dc.contributor.author","Pacile, S."],["dc.contributor.author","Nesterets, Y. I."],["dc.contributor.author","Mayo, S. C."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Dreossi, D."],["dc.contributor.author","Arfelli, F."],["dc.contributor.author","Thompson, David N."],["dc.contributor.author","Lockie, D."],["dc.contributor.author","McCormack, M. Luke"],["dc.contributor.author","Taba, S. T."],["dc.contributor.author","Brun, F."],["dc.contributor.author","Pinamonti, M."],["dc.contributor.author","Nickson, C."],["dc.contributor.author","Hall, C."],["dc.contributor.author","Dimmock, M."],["dc.contributor.author","Zanconati, F."],["dc.contributor.author","Cholewa, M."],["dc.contributor.author","Quiney, H."],["dc.contributor.author","Brennan, P. C."],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Gureyev, Timur"],["dc.date.accessioned","2018-11-07T10:26:07Z"],["dc.date.available","2018-11-07T10:26:07Z"],["dc.date.issued","2017"],["dc.description.abstract","The aim of this study was to optimise the experimental protocol and data analysis for in-vivo breast cancer x-ray imaging. Results are presented of the experiment at the SYRMEP beamline of Elettra Synchrotron using the propagation-based phase-contrast mammographic tomography method, which incorporates not only absorption, but also x-ray phase information. In this study the images of breast tissue samples, of a size corresponding to a full human breast, with radiologically acceptable x-ray doses were obtained, and the degree of improvement of the image quality (from the diagnostic point of view) achievable using propagation-based phase-contrast image acquisition protocols with proper incorporation of x-ray phase retrieval into the reconstruction pipeline was investigated. Parameters such as the x-ray energy, sample-to-detector distance and data processing methods were tested, evaluated and optimized with respect to the estimated diagnostic value using a mastectomy sample with a malignant lesion. The results of quantitative evaluation of images were obtained by means of radiological assessment carried out by 13 experienced specialists. A comparative analysis was performed between the x-ray and the histological images of the specimen. The results of the analysis indicate that, within the investigated range of parameters, both the objective image quality characteristics and the subjective radiological scores of propagation-based phase-contrast images of breast tissues monotonically increase with the strength of phase contrast which in turn is directly proportional to the product of the radiation wavelength and the sample-to-detector distance. The outcomes of this study serve to define the practical imaging conditions and the CT reconstruction procedures appropriate for low-dose phase-contrast mammographic imaging of live patients at specially designed synchrotron beamlines."],["dc.identifier.doi","10.1088/1361-6560/aa5d3d"],["dc.identifier.isi","000395886000004"],["dc.identifier.pmid","28140377"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42972"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","1361-6560"],["dc.relation.issn","0031-9155"],["dc.title","Optimization of propagation-based x-ray phase-contrast tomography for breast cancer imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1831"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","The New Phytologist"],["dc.bibliographiccitation.lastpage","1842"],["dc.bibliographiccitation.volume","221"],["dc.contributor.author","Losso, Adriano"],["dc.contributor.author","Bär, Andreas"],["dc.contributor.author","Dämon, Birgit"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Ganthaler, Andrea"],["dc.contributor.author","Petruzzellis, Francesco"],["dc.contributor.author","Savi, Tadeja"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Nardini, Andrea"],["dc.contributor.author","Mayr, Stefan"],["dc.contributor.author","Beikircher, Barbara"],["dc.date.accessioned","2020-12-10T18:36:17Z"],["dc.date.available","2020-12-10T18:36:17Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1111/nph.15549"],["dc.identifier.eissn","1469-8137"],["dc.identifier.issn","0028-646X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76572"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Insights from in vivo micro‐CT analysis: testing the hydraulic vulnerability segmentation in Acer pseudoplatanus and Fagus sylvatica seedlings"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3099"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.lastpage","3112"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Pacile, S."],["dc.contributor.author","Brun, F."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Nesterest, Y. I."],["dc.contributor.author","Dreossi, D."],["dc.contributor.author","Mohammadi, S."],["dc.contributor.author","Tonutti, M."],["dc.contributor.author","Stacul, F."],["dc.contributor.author","Lockie, D."],["dc.contributor.author","Zanconati, F."],["dc.contributor.author","Accardo, A."],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Gureyev, Timur"],["dc.date.accessioned","2018-11-07T09:53:51Z"],["dc.date.available","2018-11-07T09:53:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Results are presented of a feasibility study of three-dimensional X-ray tomographic mammography utilising in-line phase contrast. Experiments were performed at SYRMEP beamline of Elettra synchrotron. A specially designed plastic phantom and a mastectomy sample containing a malignant lesion were used to study the reconstructed image quality as a function of different image processing operations. Detailed evaluation and optimization of image reconstruction workflows have been carried out using combinations of several advanced computed tomography algorithms with different pre-processing and post-processing steps. Special attention was paid to the effect of phase retrieval on the diagnostic value of the reconstructed images. A number of objective image quality indices have been applied for quantitative evaluation of the results, and these were compared with subjective assessments of the same images by three experienced radiologists and one pathologist. The outcomes of this study provide practical guidelines for the optimization of image processing workflows in synchrotron-based phase-contrast mammo-tomography. (C) 2015 Optical Society of America"],["dc.description.sponsorship","EXTREMA COST Action [MP1207]"],["dc.identifier.doi","10.1364/BOE.6.003099"],["dc.identifier.isi","000359006000032"],["dc.identifier.pmid","26309770"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36418"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Optical Soc Amer"],["dc.relation.issn","2156-7085"],["dc.title","Clinical application of low-dose phase contrast breast CT: methods for the optimization of the reconstruction workflow"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0170597"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Ufartes, Roser"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Martin, Sabine"],["dc.contributor.author","Lazzarini, Marcio"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Pinkert-Leetsch, Diana"],["dc.contributor.author","Katschinski, Doerthe Magdalena"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T10:27:31Z"],["dc.date.available","2018-11-07T10:27:31Z"],["dc.date.issued","2017"],["dc.description.abstract","The small size of the adult and developing mouse heart poses a great challenge for imaging in preclinical research. The aim of the study was to establish a phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances the x-ray attenuation of soft-tissue to allow high resolution 3D visualization of mouse hearts by synchrotron radiation based pCT (SRpCT) and classical pCT. We demonstrate that SRpCT of PTA stained mouse hearts ex vivo allows imaging of the cardiac atrium, ventricles, myocardium especially its fibre structure and vessel walls in great detail and furthermore enables the depiction of growth and anatomical changes during distinct developmental stages of hearts in mouse embryos. Our x-ray based virtual histology approach is not limited to SRpCT as it does not require monochromatic and/or coherent x-ray sources and even more importantly can be combined with conventional histological procedures. Furthermore, it permits volumetric measurements as we show for the assessment of the plaque volumes in the aortic valve region of mice from an ApoE-/- mouse model. Subsequent, Masson-Goldner trichrome staining of paraffin sections of PTA stained samples revealed intact collagen and muscle fibres and positive staining of CD31 on endothelial cells by immunohistochemistry illustrates that our approach does not prevent immunochemistry analysis. The feasibility to scan hearts already embedded in paraffin ensured a 100% correlation between virtual cut sections of the CT data sets and histological heart sections of the same sample and may allow in future guiding the cutting process to specific regions of interest. In summary, since our CT based virtual histology approach is a powerful tool for the 3D depiction of morphological alterations in hearts and embryos in high resolution and can be combined with classical histological analysis it may be used in preclinical research to unravel structural alterations of various heart diseases."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1371/journal.pone.0170597"],["dc.identifier.isi","000393712500011"],["dc.identifier.pmid","28178293"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14241"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43247"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","mu CT of ex-vivo stained mouse hearts and embryos enables a precise match between 3D virtual histology, classical histology and immunochemistry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","143"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","155"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","dal Monego, Simeone"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Mohammadi, Sara"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Garrovo, Chiara"],["dc.contributor.author","Biffi, Stefania"],["dc.contributor.author","Lorenzon, Andrea"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Accardo, Agostino"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Tromba, Giuliana"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites."],["dc.identifier.doi","10.1107/S1600577514021730"],["dc.identifier.fs","608140"],["dc.identifier.gro","3141991"],["dc.identifier.isi","000346850200022"],["dc.identifier.pmid","25537601"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11558"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3334"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/230739/EU//P3AGI"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1106"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1111"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Markus, A. M."],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T09:55:18Z"],["dc.date.available","2018-11-07T09:55:18Z"],["dc.date.issued","2015"],["dc.description.abstract","Lung imaging in mouse disease models is crucial for the assessment of the severity of airway disease but remains challenging due to the small size and the high porosity of the organ. Synchrotron inline free-propagation phase-contrast computed tomography (CT) with its intrinsic high soft-tissue contrast provides the necessary sensitivity and spatial resolution to analyse the mouse lung structure in great detail. Here, this technique has been applied in combination with single-distance phase retrieval to quantify alterations of the lung structure in experimental asthma mouse models of different severity. In order to mimic an in vivo situation as close as possible, the lungs were inflated with air at a constant physiological pressure. Entire mice were embedded in agarose gel and imaged using inline free-propagation phase-contrast CT at the SYRMEP beamline (Synchrotron Light Source, 'Elettra', Trieste, Italy). The quantification of the obtained phase-contrast CT data sets revealed an increasing lung soft-tissue content in mice correlating with the degree of the severity of experimental allergic airways disease. In this way, it was possible to successfully discriminate between healthy controls and mice with either mild or severe allergic airway disease. It is believed that this approach may have the potential to evaluate the efficacy of novel therapeutic strategies that target airway remodelling processes in asthma."],["dc.identifier.doi","10.1107/S1600577515006177"],["dc.identifier.isi","000357407900030"],["dc.identifier.pmid","26134818"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36710"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Phase-contrast computed tomography for quantification of structural changes in lungs of asthma mouse models of different severity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","104"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.bibliographiccitation.lastpage","110"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","Petruzzellis, Francesco"],["dc.contributor.author","Pagliarani, Chiara"],["dc.contributor.author","Savi, Tadeja"],["dc.contributor.author","Losso, Adriano"],["dc.contributor.author","Cavalletto, Silvia"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Bär, Andreas"],["dc.contributor.author","Ganthaler, Andrea"],["dc.contributor.author","Miotto, Andrea"],["dc.contributor.author","Mayr, Stefan"],["dc.contributor.author","Zwieniecki, Maciej A."],["dc.contributor.author","Nardini, Andrea"],["dc.contributor.author","Secchi, Francesca"],["dc.date.accessioned","2020-12-10T18:36:16Z"],["dc.date.available","2020-12-10T18:36:16Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1111/nph.15368"],["dc.identifier.issn","0028-646X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76568"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","The pitfalls of in vivo imaging techniques: evidence for cellular damage caused by synchrotron X-ray computed micro-tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","769"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Applied Sciences"],["dc.bibliographiccitation.volume","12"],["dc.contributor.affiliation","Mensa, Francesco Simone; 1Department of Science, University of Roma Tre, 00146 Rome, Italy; francescosimone.mensa@uniroma3.it (F.S.M.); maurizio.muzzi@uniroma3.it (M.M.); f.spani@unicampus.it (F.S.)"],["dc.contributor.affiliation","Muzzi, Maurizio; 1Department of Science, University of Roma Tre, 00146 Rome, Italy; francescosimone.mensa@uniroma3.it (F.S.M.); maurizio.muzzi@uniroma3.it (M.M.); f.spani@unicampus.it (F.S.)"],["dc.contributor.affiliation","Spani, Federica; 1Department of Science, University of Roma Tre, 00146 Rome, Italy; francescosimone.mensa@uniroma3.it (F.S.M.); maurizio.muzzi@uniroma3.it (M.M.); f.spani@unicampus.it (F.S.)"],["dc.contributor.affiliation","Tromba, Giuliana; 3Elettra-Sincrotrone Trieste, 34149 Basovizza, Italy; giuliana.tromba@elettra.eu"],["dc.contributor.affiliation","Dullin, Christian; 4Institute for Diagnostic and Interventional Radiology, University Medical Center Goettingen, 37075 Goettingen, Germany; christian.dullin@med.uni-goettingen.de"],["dc.contributor.affiliation","Di Giulio, Andrea; 1Department of Science, University of Roma Tre, 00146 Rome, Italy; francescosimone.mensa@uniroma3.it (F.S.M.); maurizio.muzzi@uniroma3.it (M.M.); f.spani@unicampus.it (F.S.)"],["dc.contributor.author","Mensa, Francesco Simone"],["dc.contributor.author","Muzzi, Maurizio"],["dc.contributor.author","Spani, Federica"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Di Giulio, Andrea"],["dc.contributor.editor","Fovo, Alice Dal"],["dc.contributor.editor","Fontana, Raffaella"],["dc.date.accessioned","2022-02-01T10:31:42Z"],["dc.date.available","2022-02-01T10:31:42Z"],["dc.date.issued","2022"],["dc.date.updated","2022-02-09T13:18:33Z"],["dc.description.abstract","Many techniques are used today to study insect morphology, including light and electron microscopy. Most of them require to specifically prepare the sample, precluding its use for further investigation. In contrast, micro-CT allows a sample to be studied in a non-destructive and rapid process, even without specific treatments that might hinder the use of rare and hard-to-find species in nature. We used synchrotron radiation (SR) micro-CT and conventional micro-CT to prepare 3D reconstructions of Diptera, Coleoptera, and Hymenoptera species that had been processed with 4 common preparation procedures: critical-point drying, sputter-coating, resin embedding, and air-drying. Our results showed that it is possible to further utilize insect samples prepared with the aforementioned preparation techniques for the creation of 3D models. Specimens dried at the critical point showed the best results, allowing us to faithfully reconstruct both their external surface and their internal structures, while sputter-coated insects were the most troublesome for the 3D reconstruction procedure. Air-dried specimens were suitable for external morphological analyses, while anatomical investigation of soft internal organs was not possible due to their shrinking and collapsing. The sample included in resin allowed us to reconstruct and appreciate the external cuticle and the internal parts. In this work, we demonstrate that insect samples destined to different analyses can be used for new micro-CT studies, further deepening the possibility of state-of-the-art morphological analyses."],["dc.description.abstract","Many techniques are used today to study insect morphology, including light and electron microscopy. Most of them require to specifically prepare the sample, precluding its use for further investigation. In contrast, micro-CT allows a sample to be studied in a non-destructive and rapid process, even without specific treatments that might hinder the use of rare and hard-to-find species in nature. We used synchrotron radiation (SR) micro-CT and conventional micro-CT to prepare 3D reconstructions of Diptera, Coleoptera, and Hymenoptera species that had been processed with 4 common preparation procedures: critical-point drying, sputter-coating, resin embedding, and air-drying. Our results showed that it is possible to further utilize insect samples prepared with the aforementioned preparation techniques for the creation of 3D models. Specimens dried at the critical point showed the best results, allowing us to faithfully reconstruct both their external surface and their internal structures, while sputter-coated insects were the most troublesome for the 3D reconstruction procedure. Air-dried specimens were suitable for external morphological analyses, while anatomical investigation of soft internal organs was not possible due to their shrinking and collapsing. The sample included in resin allowed us to reconstruct and appreciate the external cuticle and the internal parts. In this work, we demonstrate that insect samples destined to different analyses can be used for new micro-CT studies, further deepening the possibility of state-of-the-art morphological analyses."],["dc.identifier.doi","10.3390/app12020769"],["dc.identifier.eissn","2076-3417"],["dc.identifier.pii","app12020769"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98927"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-3417"],["dc.rights","Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."],["dc.title","When the Utility of Micro-Computed Tomography Collides with Insect Sample Preparation: An Entomologist User Guide to Solve Post-Processing Issues and Achieve Optimal 3D Models"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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