Dullin, Christian

[["dc.bibliographiccitation.firstpage","755"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Neoplasia (New York, N.Y.)"],["dc.bibliographiccitation.lastpage","765"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Domeyer-Missbach, Melanie"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Obenauer, Silvia"],["dc.contributor.author","Kauer, Fritz"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Vogel, Wolfgang F."],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-10T08:11:50Z"],["dc.date.available","2019-07-10T08:11:50Z"],["dc.date.issued","2007-09-01"],["dc.description.abstract","Skeletal metastasis is an important cause of mortality in patients with breast cancer. Hence, animal models, in combination with various imaging techniques, are in high demand for preclinical assessment of novel therapies. We evaluated the applicability of flat-panel volume computed tomography (fpVCT) to noninvasive detection of osteolytic bone metastases that develop in severe immunodeficient mice after intracardial injection of MDA-MB-231 breast cancer cells. A single fpVCT scan at 200-microm isotropic resolution was employed to detect osteolysis within the entire skeleton. Osteolytic lesions identified by fpVCT correlated with Faxitron X-ray analysis and were subsequently confirmed by histopathological examination. Isotropic three-dimensional image data sets obtained by fpVCT were the basis for the precise visualization of the extent of the lesion within the cortical bone and for the measurement of bone loss. Furthermore, fpVCT imaging allows continuous monitoring of growth kinetics for each metastatic site and visualization of lesions in more complex regions of the skeleton, such as the skull. Our findings suggest that fpVCT is a powerful tool that can be used to monitor the occurrence and progression of osteolytic lesions in vivo and can be further developed to monitor responses to antimetastatic therapies over the course of the disease."],["dc.identifier.pmid","17898871"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11246"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60807"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1476-5586"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Bone Neoplasms"],["dc.subject.mesh","Breast Neoplasms"],["dc.subject.mesh","Disease Progression"],["dc.subject.mesh","Female"],["dc.subject.mesh","Femoral Neoplasms"],["dc.subject.mesh","Humerus"],["dc.subject.mesh","Imaging, Three-Dimensional"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Mice, SCID"],["dc.subject.mesh","Models, Animal"],["dc.subject.mesh","Osteolysis"],["dc.subject.mesh","Skull Neoplasms"],["dc.subject.mesh","Specific Pathogen-Free Organisms"],["dc.subject.mesh","Tibia"],["dc.subject.mesh","Tomography, X-Ray Computed"],["dc.subject.mesh","Tumor Burden"],["dc.title","Flat-panel detector-based volume computed tomography: a novel 3D imaging technique to monitor osteolytic bone lesions in a mouse tumor metastasis model."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","09973"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:24Z"],["dc.date.available","2017-09-07T11:44:24Z"],["dc.date.issued","2015"],["dc.description.abstract","We have performed x-ray phase-contrast tomography on mouse lung tissue. Using a divergent x-ray beam generated by nanoscale focusing, we used zoom tomography to produce three-dimensional reconstructions with selectable magnification, resolution, and field of view. Thus, macroscopic tissue samples extending over several mm can be studied in sub-cellular-level structural detail. The zoom capability and, in particular, the high dose efficiency are enabled by the near-perfect exit wavefront of an optimized x-ray waveguide channel. In combination with suitable phase-retrieval algorithms, challenging radiation-sensitive and low-contrast samples can be reconstructed with minimal artefacts. The dose efficiency of the method is demonstrated by the reconstruction of living macrophages both with and without phagocytized contrast agents. We also used zoom tomography to visualize barium-labelled macrophages in the context of morphological structures in asthmatic and healthy mouse lung tissue one day after intratracheal application. The three-dimensional reconstructions showed that the macrophages predominantly localized to the alveoli, but they were also found in bronchial walls, indicating that these cells might be able to migrate from the lumen of the bronchi through the epithelium."],["dc.identifier.doi","10.1038/srep09973"],["dc.identifier.fs","615791"],["dc.identifier.gro","3141904"],["dc.identifier.isi","000354310200001"],["dc.identifier.pmid","25966338"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13625"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2367"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Cell Line, Transformed"],["dc.subject.mesh","Cell Movement"],["dc.subject.mesh","Macrophages, Alveolar"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Pulmonary Alveoli"],["dc.subject.mesh","Respiratory Mucosa"],["dc.subject.mesh","Tomography, X-Ray"],["dc.title","Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]