Schottdorf, Manuel

[["dc.bibliographiccitation.firstpage","194"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","203"],["dc.bibliographiccitation.volume","257"],["dc.contributor.author","Samhaber, Robert"],["dc.contributor.author","Schottdorf, Manuel"],["dc.contributor.author","El Hady, Ahmed"],["dc.contributor.author","Broeking, Kai"],["dc.contributor.author","Daus, Andreas"],["dc.contributor.author","Thielemann, Christiane"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Wolf, Fred"],["dc.date.accessioned","2018-11-07T10:19:24Z"],["dc.date.available","2018-11-07T10:19:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: Multi-electrode arrays (MEAs) allow non-invasive multi-unit recording in-vitro from cultured neuronal networks. For sufficient neuronal growth and adhesion on such MEAs, substrate preparation is required. Plating of dissociated neurons on a uniformly prepared MEA's surface results in the formation of spatially extended random networks with substantial inter-sample variability. Such cultures are not optimally suited to study the relationship between defined structure and dynamics in neuronal networks. To overcome these shortcomings, neurons can be cultured with pre-defined topology by spatially structured surface modification. Spatially structuring a MEA surface accurately and reproducibly with the equipment of a typical cell-culture laboratory is challenging. New method: In this paper, we present a novel approach utilizing micro-contact printing (mu CP) combined with a custom-made device to accurately position patterns on MEAs with high precision. We call this technique AP-mu CP (accurate positioning micro-contact printing). Comparison with existing methods: Other approaches presented in the literature using mu CP for patterning either relied on facilities or techniques not readily available in a standard cell culture laboratory, or they did not specify means of precise pattern positioning. Conclusion: Here we present a relatively simple device for reproducible and precise patterning in a standard cell-culture laboratory setting. The patterned neuronal islands on MEAs provide a basis for high throughput electrophysiology to study the dynamics of single neurons and neuronal networks. (C) 2015 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jneumeth.2015.09.022"],["dc.identifier.isi","000366224100020"],["dc.identifier.pmid","26432934"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41649"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1872-678X"],["dc.relation.issn","0165-0270"],["dc.title","Growing neuronal islands on multi-electrode arrays using an accurate positioning-mu CP device"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

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[["dc.contributor.author","Samhaber, Robert"],["dc.contributor.author","Schottdorf, Manuel"],["dc.contributor.author","Hady, Ahmed El"],["dc.contributor.author","Broeking, Kai"],["dc.contributor.author","Daus, Andreas"],["dc.contributor.author","Thielemann, Christiane"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Wolf, Fred"],["dc.date.accessioned","2017-09-07T11:45:41Z"],["dc.date.available","2017-09-07T11:45:41Z"],["dc.date.issued","2015"],["dc.identifier.gro","3151836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8663"],["dc.notes.status","public"],["dc.notes.submitter","chake"],["dc.publisher","Cold Spring Harbor Laboratory"],["dc.title","Growing neuronal islands on multi-electrode arrays using an Accurate Positioning-μCP device"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","733"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","741.e7"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Ho, Chun Lum Andy"],["dc.contributor.author","Zimmermann, Robert"],["dc.contributor.author","Flórez Weidinger, Juan Daniel"],["dc.contributor.author","Prsa, Mario"],["dc.contributor.author","Schottdorf, Manuel"],["dc.contributor.author","Merlin, Sam"],["dc.contributor.author","Okamoto, Tsuyoshi"],["dc.contributor.author","Ikezoe, Koji"],["dc.contributor.author","Pifferi, Fabien"],["dc.contributor.author","Huber, Daniel"],["dc.date.accessioned","2021-06-01T09:41:15Z"],["dc.date.available","2021-06-01T09:41:15Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.cub.2020.11.027"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84858"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0960-9822"],["dc.title","Orientation Preference Maps in Microcebus murinus Reveal Size-Invariant Design Principles in Primate Visual Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]