Frohn, Jasper

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Scandinavian Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","1267"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Andersson, Mariam"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Robisch, Anna-Lena"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:23:03Z"],["dc.date.available","2021-04-14T08:23:03Z"],["dc.date.issued","2020"],["dc.description.abstract","Objectives: Light microscopical analysis in two dimensions, combined with immunohistochemistry, is\r\npresently the gold standard to describe the enteric nervous system (ENS). Our aim was to assess the\r\nusefulness of three-dimensional (3D) imaging by X-ray phase-contrast tomography in evaluating the\r\nENS of the human bowel.\r\nMaterial and methods: Myenteric ganglia were identified in full-thickness biopsies of the ileum and\r\ncolon by hematoxylin & eosin staining. A1-mm biopsy punch was taken from the paraffin blocks and\r\nplaced into a KaptonVR tube for subsequent tomographic investigation. The samples were scanned,\r\nwithout further preparation, using phase-contrast tomography at two different scales: overview scans\r\n(performed with laboratory setups), which allowed localization of the nervous tissue ( 1mm effective\r\nvoxel size); and high-resolution scans (performed with a synchrotron endstation), which imaged localized\r\nregions of 320x320x320 mm3 (176 nm effective voxel size).\r\nResults: The contrast allowed us to follow the shape and the size changes of the ganglia, as well as\r\nto study their cellular components together with the cells and cellular projections of the periganglional\r\nspace. Furthermore, it was possible to show the 3D network of the myenteric plexus and to quantify\r\nits volume within the samples.\r\nConclusions: Phase-contrast X-ray tomography can be applied for volume analyses of the human ENS\r\nand to study tissue components in unstained paraffin-embedded tissue biopsies. This technique could\r\npotentially be used to study disease mechanisms, and to compare healthy and diseased tissues in clinical\r\nresearch."],["dc.identifier.doi","10.1080/00365521.2020.1815079"],["dc.identifier.pmid","32907418"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80786"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/70"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1502-7708"],["dc.relation.issn","0036-5521"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kuehnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-06-26T11:04:13Z"],["dc.date.available","2020-06-26T11:04:13Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a new approach of three-dimensional (3d) virtual histology and patho-histology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal-architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we can propose multi-scale phase contrast x-ray tomography as a novel tool to unravel the patho-physiology of Covid-19, extending conventional histology by a third dimension and allowing for a full quantification of tissue remodeling.By combining parallel and cone beam geometry, autopsy samples with a cross section of 4mm are scanned and reconstructed at a resolution and image quality which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 160nm. We exemplify the capability of this approach by 3d visualisation of the diffuse alveolar damage with its prominent hyaline membrane formation, by mapping the 3d distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.identifier.doi","10.1101/2020.06.21.20134882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66752"],["dc.language.iso","en"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.gro","other"],["dc.title","3d Virtual Patho-Histology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Skin Research & Technology"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Englund, Elisabet"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:31:27Z"],["dc.date.available","2021-04-14T08:31:27Z"],["dc.date.issued","2020"],["dc.description.abstract","BackgroundEnteric neuropathy is described in most patients with gastrointestinal dysmotility and may be found together with reduced intraepidermal nerve fiber density (IENFD). The aim of this pilot study was to assess whether three-dimensional (3d) imaging of skin biopsies could be used to examine various tissue components in patients with gastrointestinal dysmotility.Material and methodsFour dysmotility patients of different etiology and two healthy volunteers were included. From each subject, two 3-mm punch skin biopsies were stained with antibodies against protein gene product 9.5 or evaluated as a whole with two X-ray phase-contrast computed tomography (CT) setups, a laboratory µCT setup and a dedicated synchrotron radiation nanoCT end-station.ResultsTwo patients had reduced IENFD, and two normal IENFD, compared with controls. µCT and X-ray phase-contrast holographic nanotomography scanned whole tissue specimens, with optional high-resolution scans revealing delicate structures, without differentiation of various fibers and cells. Irregular architecture of dermal fibers was observed in the patient with Ehlers-Danlos syndrome and the patient with idiopathic dysmotility showed an abundance of mesenchymal ground substance.Conclusions3d phase-contrast tomographic imaging may be useful to illustrate traits of connective tissue dysfunction in various organs and to demonstrate whether disorganized dermal fibers could explain organ dysfunction."],["dc.identifier.doi","10.1111/srt.12974"],["dc.identifier.pmid","33022848"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83598"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/82"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1600-0846"],["dc.relation.issn","0909-752X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3d phase‐contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e60408"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kühnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2021-03-01T08:45:14Z"],["dc.date.available","2021-03-01T08:45:14Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.7554/eLife.60408"],["dc.identifier.pmid","32815517"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17619"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79864"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/162"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.gro","biomedical tomography"],["dc.title","3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]