Frohn, Jasper

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Scandinavian Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","1267"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Andersson, Mariam"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Robisch, Anna-Lena"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:23:03Z"],["dc.date.available","2021-04-14T08:23:03Z"],["dc.date.issued","2020"],["dc.description.abstract","Objectives: Light microscopical analysis in two dimensions, combined with immunohistochemistry, is\r\npresently the gold standard to describe the enteric nervous system (ENS). Our aim was to assess the\r\nusefulness of three-dimensional (3D) imaging by X-ray phase-contrast tomography in evaluating the\r\nENS of the human bowel.\r\nMaterial and methods: Myenteric ganglia were identified in full-thickness biopsies of the ileum and\r\ncolon by hematoxylin & eosin staining. A1-mm biopsy punch was taken from the paraffin blocks and\r\nplaced into a KaptonVR tube for subsequent tomographic investigation. The samples were scanned,\r\nwithout further preparation, using phase-contrast tomography at two different scales: overview scans\r\n(performed with laboratory setups), which allowed localization of the nervous tissue ( 1mm effective\r\nvoxel size); and high-resolution scans (performed with a synchrotron endstation), which imaged localized\r\nregions of 320x320x320 mm3 (176 nm effective voxel size).\r\nResults: The contrast allowed us to follow the shape and the size changes of the ganglia, as well as\r\nto study their cellular components together with the cells and cellular projections of the periganglional\r\nspace. Furthermore, it was possible to show the 3D network of the myenteric plexus and to quantify\r\nits volume within the samples.\r\nConclusions: Phase-contrast X-ray tomography can be applied for volume analyses of the human ENS\r\nand to study tissue components in unstained paraffin-embedded tissue biopsies. This technique could\r\npotentially be used to study disease mechanisms, and to compare healthy and diseased tissues in clinical\r\nresearch."],["dc.identifier.doi","10.1080/00365521.2020.1815079"],["dc.identifier.pmid","32907418"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80786"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/70"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1502-7708"],["dc.relation.issn","0036-5521"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","235034"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Physics in Medicine and Biology"],["dc.bibliographiccitation.volume","65"],["dc.contributor.affiliation","Robisch, A-L;"],["dc.contributor.affiliation","Frohn, J;"],["dc.contributor.affiliation","Salditt, T;"],["dc.contributor.author","Robisch, A.-L."],["dc.contributor.author","Frohn, J."],["dc.contributor.author","Salditt, T."],["dc.date.accessioned","2021-04-14T08:25:12Z"],["dc.date.available","2021-04-14T08:25:12Z"],["dc.date.issued","2020"],["dc.date.updated","2022-02-09T13:18:22Z"],["dc.description.abstract","AbstractThree-dimensional reconstruction from truncated two-dimensional projections cannot be solved analytically without prior knowledge of the sample or access to the non-truncated projections. To suppress reconstruction artifacts in region of interest (ROI) or local tomography, an iterative algorithm has been devised based on back-projection and re-projection, assuming an approximately cylindrical shape of the entire sample of general homogeneity, which is especially applicable for micro-tomography of biopsy samples from biological tissue. Tomographic reconstruction is iteratively refined by minimizing the mismatch between an empty ROI and the reconstruction from the difference between measured sinogram and forward projected ROI reconstruction. By numerical simulation and experimental demonstration, it is shown that the algorithm is not only able to reconstruct quantitative gray values, but also to reduce artifacts of peripheral glow, and may lead to increased image sharpness. The method is particularly suitable for examination of biopsy/autopsy-samples of soft tissue by micro/nano-computed tomography."],["dc.description.sponsorship","Bundesministerium für Bildung und Forschunghttp://dx.doi.org/10.13039/501100002347"],["dc.identifier.doi","10.1088/1361-6560/abc22f"],["dc.identifier.eissn","1361-6560"],["dc.identifier.issn","0031-9155"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81554"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1361-6560"],["dc.relation.issn","0031-9155"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Iterative micro-tomography of biopsy samples from truncated projections with quantitative gray values"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","6945"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Quade, Felix Simon Christian"],["dc.contributor.author","Holtzheimer, Jana"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Prpic, Nikola Michael"],["dc.date.accessioned","2019-07-09T11:51:39Z"],["dc.date.available","2019-07-09T11:51:39Z"],["dc.date.issued","2019"],["dc.description.abstract","Spiders have evolved a unique male copulatory organ, the pedipalp bulb. The morphology of the bulb is species specific and plays an important role in species recognition and prezygotic reproductive isolation. Despite its importance for spider biodiversity, the mechanisms that control bulb development are virtually unknown. We have used confocal laser scanning microscopy (CLSM) and diffusible iodine-based contrast-enhanced micro computed tomography (dice-µCT) to study bulb development in the spider Parasteatoda tepidariorum. These imaging technologies enabled us to study bulb development in situ, without the use of destructive procedures for the first time. We show here that the inflated pedipalp tip in the subadult stage is filled with haemolymph that rapidly coagulates. Coagulation indicates histolytic processes that disintegrate tibia and tarsus, similar to histolytic processes during metamorphosis in holometabolous insects. The coagulated material contains cell inclusions that likely represent the cell source for the re-establishment of tarsus and tibia after histolysis, comparable to the histoblasts in insect metamorphosis. The shape of the coagulated mass prefigures the shape of the adult tarsus (cymbium) like a blueprint for the histoblasts. This suggests a unique role for controlled coagulation after histolysis in the metamorphosis-like morphogenesis of the male pedipalp."],["dc.identifier.doi","10.1038/s41598-019-43192-9"],["dc.identifier.pmid","31061504"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16163"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59982"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","570"],["dc.subject.gro","biomedical tomography"],["dc.title","Formation and development of the male copulatory organ in the spider Parasteatoda tepidariorum involves a metamorphosis-like process"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Skin Research & Technology"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Englund, Elisabet"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:31:27Z"],["dc.date.available","2021-04-14T08:31:27Z"],["dc.date.issued","2020"],["dc.description.abstract","BackgroundEnteric neuropathy is described in most patients with gastrointestinal dysmotility and may be found together with reduced intraepidermal nerve fiber density (IENFD). The aim of this pilot study was to assess whether three-dimensional (3d) imaging of skin biopsies could be used to examine various tissue components in patients with gastrointestinal dysmotility.Material and methodsFour dysmotility patients of different etiology and two healthy volunteers were included. From each subject, two 3-mm punch skin biopsies were stained with antibodies against protein gene product 9.5 or evaluated as a whole with two X-ray phase-contrast computed tomography (CT) setups, a laboratory µCT setup and a dedicated synchrotron radiation nanoCT end-station.ResultsTwo patients had reduced IENFD, and two normal IENFD, compared with controls. µCT and X-ray phase-contrast holographic nanotomography scanned whole tissue specimens, with optional high-resolution scans revealing delicate structures, without differentiation of various fibers and cells. Irregular architecture of dermal fibers was observed in the patient with Ehlers-Danlos syndrome and the patient with idiopathic dysmotility showed an abundance of mesenchymal ground substance.Conclusions3d phase-contrast tomographic imaging may be useful to illustrate traits of connective tissue dysfunction in various organs and to demonstrate whether disorganized dermal fibers could explain organ dysfunction."],["dc.identifier.doi","10.1111/srt.12974"],["dc.identifier.pmid","33022848"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83598"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/82"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1600-0846"],["dc.relation.issn","0909-752X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3d phase‐contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2633"],["dc.bibliographiccitation.firstpage","2633"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Khan, Amara"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-12-10T18:42:01Z"],["dc.date.available","2020-12-10T18:42:01Z"],["dc.date.issued","2020"],["dc.description.abstract","The spatial organization of cardiac muscle tissue exhibits a complex structure on multiple length scales, from the sarcomeric unit to the whole organ. Here we demonstrate a multi-scale three-dimensional imaging (3d) approach with three levels of magnification, based on synchrotron X-ray phase contrast tomography. Whole mouse hearts are scanned in an undulator beam, which is first focused and then broadened by divergence. Regions-of-interest of the hearts are scanned in parallel beam as well as a biopsy by magnified cone beam geometry using a X-ray waveguide optic. Data is analyzed in terms of orientation, anisotropy and the sarcomeric periodicity via a local Fourier transformation."],["dc.identifier.doi","10.1364/BOE.386576"],["dc.identifier.pmid","32499949"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17770"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77772"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/192"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2156-7085"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","biomedical tomography"],["dc.title","Multi-scale X-ray phase-contrast tomography of murine heart tissue"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e60408"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kühnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2021-03-01T08:45:14Z"],["dc.date.available","2021-03-01T08:45:14Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.7554/eLife.60408"],["dc.identifier.pmid","32815517"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17619"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79864"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/162"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.gro","biomedical tomography"],["dc.title","3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1707"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1719"],["dc.bibliographiccitation.volume","27"],["dc.contributor.affiliation","Frohn, Jasper; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Pinkert-Leetsch, Diana; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.affiliation","Missbach-Güntner, Jeannine; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.affiliation","Reichardt, Marius; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Osterhoff, Markus; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Alves, Frauke; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Pinkert-Leetsch, Diana"],["dc.contributor.author","Missbach-Güntner, Jeannine"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2020-12-03T08:19:06Z"],["dc.date.available","2020-12-03T08:19:06Z"],["dc.date.issued","2020-10-26"],["dc.date.updated","2022-02-09T13:21:33Z"],["dc.description.abstract","A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline P10/PETRA III (DESY, Hamburg, Germany). This enables the 3D reconstruction of characteristic morphological features of human pancreatic normal and tumor tissue, as obtained from cancer surgery, first in the form of a large-scale overview by parallel-beam illumination, followed by a zoom into a region-of-interest based on zoom tomography using a Kirkpatrick-Baez mirror with additional waveguide optics. To this end 1 mm punch biopsies of the tissue were taken. In the parallel tomography, a volumetric throughput on the order of 0.01 mm3 s-1 was achieved, while maintaining the ability to segment isolated cells. With a continuous rotation during the scan, a total acquisition time of less than 2 min was required for a full tomographic scan. Using the combination of both setups, islets of Langerhans, a three-dimensional cluster of cells in the endocrine part of the pancreas, could be located. Cells in such an islet were segmented and visualized in 3D. Further, morphological alterations of tumorous tissue of the pancreas were characterized. To this end, the anisotropy parameter Ω, based on intensity gradients, was used in order to quantify the presence of collagen fibers within the entire biopsy specimen. This proof-of-concept experiment of the multiscale approach on human pancreatic tissue paves the way for future 3D virtual pathology."],["dc.description.abstract","This paper presents propagation‐based phase‐contrast tomography in two configurations at the beamline endstation GINIX, demonstrated on the application of 1 mm human pancreatic tumor tissue biopsies. image"],["dc.identifier.doi","10.1107/S1600577520011327"],["dc.identifier.pmid","33147198"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/69419"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/85"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","International Union of Crystallography"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use,\r\n distribution and reproduction in any medium, provided the original work is properly cited."],["dc.subject.gro","biomedical tomography"],["dc.title","3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]