Fölling, Jonas

[["dc.bibliographiccitation.firstpage","6266"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","6270"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kunetsky, R."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:52Z"],["dc.date.available","2017-09-07T11:49:52Z"],["dc.date.issued","2007"],["dc.description.abstract","Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed."],["dc.identifier.doi","10.1002/anie.200702167"],["dc.identifier.gro","3143552"],["dc.identifier.isi","000249114700006"],["dc.identifier.pmid","17640007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1078"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1433-7851"],["dc.title","Photochromic rhodamines provide nanoscopy with optical sectioning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","275"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Dyba, Marcus"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:54Z"],["dc.date.available","2017-09-07T11:49:54Z"],["dc.date.issued","2006"],["dc.description.abstract","We demonstrate the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by photoswitching ensembles of optically bistable organic molecular markers from a non-fluorescent to a fluorescent state and back. The photoswitching is accomplished by an isomerization reaction of a photochromic compound serving as a reversible energy acceptor of a fluorescent compound. The surpassing of the diffraction barrier with power levels of only a few hundred W cm(-2) of continuous wave irradiation is evidenced both in the effective point spread function and in the fluorescence images of test samples."],["dc.identifier.doi","10.1088/1367-2630/8/11/275"],["dc.identifier.gro","3143590"],["dc.identifier.isi","000242548900003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1120"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1367-2630"],["dc.title","Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","945"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:48:10Z"],["dc.date.available","2017-09-07T11:48:10Z"],["dc.date.issued","2008"],["dc.description.abstract","We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach."],["dc.identifier.doi","10.1038/nmeth.1257"],["dc.identifier.gro","3143218"],["dc.identifier.isi","000260532500012"],["dc.identifier.pmid","18794861"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/708"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Fluorescence nanoscopy by ground-state depletion and single-molecule return"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2463"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","2468"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Boyarskiy, Vadim P."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:15Z"],["dc.date.available","2017-09-07T11:48:15Z"],["dc.date.issued","2008"],["dc.description.abstract","By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques."],["dc.identifier.doi","10.1021/nl801471d"],["dc.identifier.gro","3143264"],["dc.identifier.isi","000258440700061"],["dc.identifier.pmid","18642961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/759"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","321"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","326"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Riedel, Dietmar"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:47Z"],["dc.date.available","2017-09-07T11:48:47Z"],["dc.date.issued","2008"],["dc.description.abstract","During the lost decade for-field fluorescence microscopy methods have evolved that have resolution for below the wavelength of light. To outperform the limiting role of diffraction, all these methods, in one way or another, switch the ability of a molecule to emit fluorescence. Here we present a novel rhodamine amide that can be photoswitched from a nonfluorescent to a fluorescent state by absorption of one or two photons from a continuous-wave loser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precision down to 15 nm in the focal plane. Two-photon induced nonlinear photoswitching of this marker with continuous-wave illumination offers optical sectioning with simple loser equipment. Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning."],["dc.identifier.doi","10.1002/cphc.200700655"],["dc.identifier.gro","3143351"],["dc.identifier.isi","000253177700017"],["dc.identifier.pmid","18200483"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/855"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1439-4235"],["dc.title","Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4249"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","4252"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Bierwagen, Jakob"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:45:17Z"],["dc.date.available","2017-09-07T11:45:17Z"],["dc.date.issued","2010"],["dc.description.abstract","We demonstrate far-field optical imaging at the nanoscale with unlabeled samples Subdiffraaion resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on off cycle to yield an image, a condition fulfilled by various biomolecules The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts"],["dc.identifier.doi","10.1021/nl1027638"],["dc.identifier.gro","3142853"],["dc.identifier.isi","000282727600075"],["dc.identifier.pmid","20831171"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/303"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Far-Field Autofluorescence Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10762"],["dc.bibliographiccitation.issue","41"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","10776"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Boyarskiy, Vadim P."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:47:36Z"],["dc.date.available","2017-09-07T11:47:36Z"],["dc.date.issued","2009"],["dc.description.abstract","The design, synthesis, and evaluation of new rhodamine spiroamides are described. These molecules have applications in optical nanoscopy based on random switching of the fluorescent single molecules. The new markers may be used in (co)localization studies of various objects and their (mutual) positions and shape can be determined with a precision of a few tens of nanometers. Multicolor staining, good photoactivation, a large number of emitted photons, and selective chemical binding with amino or thiol groups were achieved due to the presence of various functional groups on the rhodamine spiroamides. Rigidized sulfonated xanthene fragment fused with six-membered rings, N,N'-bis(2,2,2-trifluoroethyl) groups, and a combination of additional double bonds and sulfonic acid groups with simple aliphatic spiroamide residue provide multicolor properties and improve performance of the rhodamine spiroamides in photoactivation and bioconjugation reactions. Having both essential parts of the photoswitchable assembly-the switching and the fluorescent (reporter) groups-combined in one chemical entity make this approach attractive for further development. A series of rhodamine spiroamides is presented along with characterizations of their most relevant properties for application as fluorescent probes in single-molecule switching and localization microscopy. Optical images with resolutions on the nanometer scale illustrate the potential of the labels in the colocalization of biological objects and the two-photon activation technique with optical sectioning."],["dc.identifier.doi","10.1002/chem.200901333"],["dc.identifier.gro","3143166"],["dc.identifier.isi","000271605900013"],["dc.identifier.pmid","19760719"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/650"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0947-6539"],["dc.title","Rhodamine Spiroamides for Multicolor Single-Molecule Switching Fluorescent Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2686"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2694"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Rothermel, Ellen"],["dc.contributor.author","Middendorff, Claas von"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:45:15Z"],["dc.date.available","2017-09-07T11:45:15Z"],["dc.date.issued","2010"],["dc.description.abstract","Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for such transient fluorescence inhibition is the depletion of the fluorophore ground state by transferring it (via a triplet) in a dark state, a mechanism which is workable in most standard dyes. Here we show that microscopy based on ground state depletion followed by individual molecule return (GSDIM) can effectively provide multicolor diffraction-unlimited resolution imaging of immunolabeled fixed and SNAP-tag labeled living cells. Implemented with standard labeling techniques, GSDIM is demonstrated to separate up to four different conventional fluorophores using just two detection channels and a single laser line. The method can be expanded to even more colors by choosing optimized dichroic mirrors and selecting marker molecules with negligible inhomogeneous emission broadening."],["dc.identifier.doi","10.1016/j.bpj.2010.08.012"],["dc.identifier.gro","3142841"],["dc.identifier.isi","000283412500036"],["dc.identifier.pmid","20959110"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/289"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1035"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","1040"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:48:13Z"],["dc.date.available","2017-09-07T11:48:13Z"],["dc.date.issued","2008"],["dc.description.abstract","Fluorescent proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state are important for innovative microscopy schemes, such as protein tracking(1), fluorescence resonance energy transfer imaging(2), sub-diffraction resolution microscopy(3-9) and others. However, all available monomeric reversibly switchable fluorescent proteins (RSFPs) have similar properties and switching characteristics(10-12), thereby limiting their use. Here, we introduce two bright green fluorescent RSFPs, bsDronpa and Padron, generated by extensive mutagenesis of the RSFP Dronpa(10), with unique absorption and switching characteristics. Whereas bsDronpa features a broad absorption spectrum extending into the UV, Padron displays a switching behavior that is reversed to that of all green fluorescent RSFPs known to date. These two RSFPs enable live-cell fluorescence microscopy with multiple labels using a single detection color, because they can be distinguished by photoswitching. Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm."],["dc.identifier.doi","10.1038/nbt.1493"],["dc.identifier.gro","3143249"],["dc.identifier.isi","000259074700027"],["dc.identifier.pmid","18724362"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/742"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1087-0156"],["dc.title","Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","134"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Small"],["dc.bibliographiccitation.lastpage","142"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Blaaderen, Alfons van"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:49Z"],["dc.date.available","2017-09-07T11:48:49Z"],["dc.date.issued","2008"],["dc.description.abstract","We have designed and synthesized a new functional (amino reactive) highly efficient fluorescent molecular switch (FMS) with a photochromic diarylethene and a rhodamine fluorescent dye. The reactive group in this FMS -N-hydroxysuccinimide ester- allows selective labeling of amino containing molecules or other materials. In ethanolic solutions, the, compound displays a large fluorescent quantum yield of 52% and a large fluorescence modulation ratio (94%) between two states that may be interconverted with red and near-UV light. Silica nanoparticles incorporating the new FMS were prepared and characterized, and their spectroscopic and switching properties were also studied. The dye retained its properties after the incorporation into the silica, thereby allowing light-induced reversible high modulation of the fluorescence signal of a single particle for up to 60 cycles, before undergoing irreversible photobleaching. Some applications of these particles in fluorescence microscopy are also demonstrated. In particular, subdiffraction images of nanoparticles were obtained, in the focal plane of a confocal microscope."],["dc.identifier.doi","10.1002/smll.200700440"],["dc.identifier.gro","3143389"],["dc.identifier.isi","000253778100021"],["dc.identifier.pmid","18064615"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/897"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1613-6810"],["dc.title","Synthesis and characterization of photoswitchable fluorescent silica nanoparticles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]