Enderlein, Jörg

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[["dc.bibliographiccitation.firstpage","061302"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","APL Photonics"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:43Z"],["dc.date.available","2021-03-05T08:58:43Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1063/5.0009904"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80225"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/175"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2378-0967"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Fluorescence polarization filtering for accurate single molecule localization"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Jungblut, Marvin"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Schnermann, Martin"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:43Z"],["dc.date.available","2022-01-12T16:50:43Z"],["dc.date.issued","2021-12-22"],["dc.description.abstract","Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously."],["dc.format.extent","16"],["dc.identifier.doi","10.1101/2021.12.20.473473"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98094"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/378"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3494"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry. A, Molecules, spectroscopy, kinetics, environment & general theory"],["dc.bibliographiccitation.lastpage","3500"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Weber, André"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-12-10T15:22:42Z"],["dc.date.available","2020-12-10T15:22:42Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acs.jpca.0c01513"],["dc.identifier.pmid","32255633"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73500"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/39"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Wide-Field Fluorescence Lifetime Imaging of Single Molecules"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14190"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","14200"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:21Z"],["dc.date.available","2021-03-05T08:58:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acsnano.0c07322"],["dc.identifier.pmid","33035050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80100"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/79"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:38Z"],["dc.date.available","2022-01-12T16:50:38Z"],["dc.date.issued","2020"],["dc.description.abstract","Fluorescence lifetime imaging microscopy (FLIM) is an important technique that adds another dimension to the intensity and colour information of conventional microscopy. In particular, it allows for multiplexing fluorescent labels that have otherwise similar spectral properties. Currently, the only super-resolution technique that is capable of recording super-resolved images with lifetime information is STimulated Emission Depletion (STED) microscopy. In contrast, all Single-Molecule Localisation Microscopy (SMLM) techniques that employ wide-field cameras completely lack the lifetime dimension. Here, we combine Fluorescence-Lifetime Confocal Laser-Scanning Microscopy (FL-CLSM) with SMLM for realising single-molecule localisation-based fluorescence-lifetime super-resolution imaging (FL-SMLM). Besides yielding images with a spatial resolution much beyond the diffraction limit, it determines the fluorescence lifetime of all localised molecules. We validate our technique by applying it to direct STochastic Optical Reconstruction Microscopy (dSTORM) and Points Accumulation for Imaging in Nanoscale Topography (PAINT) imaging of fixed cells, and we demonstrate its multiplexing capability on samples with two different labels that differ only by fluorescence lifetime but not by their spectral properties."],["dc.format.extent","30"],["dc.identifier.doi","10.1101/2020.08.25.266387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98093"],["dc.language.iso","en"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.title","Confocal Laser-Scanning Fluorescence-Lifetime Single-Molecule Localisation Microscopy"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]