Enderlein, Jörg

[["dc.bibliographiccitation.firstpage","860"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","865"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Ghosh, Arindam"],["dc.contributor.author","Sharma, Akshita"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-12-10T18:09:58Z"],["dc.date.available","2020-12-10T18:09:58Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41566-019-0510-7"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73814"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/21"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Graphene-based metal-induced energy transfer for sub-nanometre optical localization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","letter_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","175"],["dc.bibliographiccitation.lastpage","204"],["dc.bibliographiccitation.seriesnr","518"],["dc.contributor.author","Pieper, Christoph"],["dc.contributor.author","Weiß, Kerstin"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:49:29Z"],["dc.date.available","2018-04-23T11:49:29Z"],["dc.date.issued","2013"],["dc.description.abstract","This chapter introduces into the technique of dual-focus fluorescence correlation spectroscopy or 2fFCS. In 2fFCS, the fluorescence signals generated in two laterally shifted but overlapping focal regions are auto- and crosscorrelated. The resulting correlation curves are then used to determine diffusion coefficients of fluorescent molecules or particles in solutions or membranes. Moreover, the technique can also be used for noninvasively measuring flow-velocity profiles in three dimensions. Because the distance between the focal regions is precisely known and not changed by most optical aberrations, this provides an accurate and immutable external length scale for determining diffusivities and velocities, making 2fFCS the method of choice for accurately measuring absolute values of these quantities at pico- to nanomolar concentration."],["dc.identifier.doi","10.1016/b978-0-12-388422-0.00008-x"],["dc.identifier.gro","3142130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13711"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103104"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.publisher","Elsevier"],["dc.relation.crisseries","Methods in Enzymology"],["dc.relation.isbn","978-0-12-388422-0"],["dc.relation.ispartof","Methods in Enzymology"],["dc.relation.issn","0076-6879"],["dc.title","Dual-Focus Fluorescence Correlation Spectroscopy"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","164"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","170"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Patra, Digambara"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:06Z"],["dc.date.available","2021-03-05T08:58:06Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1002/cphc.200400319"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80005"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.title","Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous-Wave and Pulsed Excitation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1472"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry Letters"],["dc.bibliographiccitation.lastpage","1475"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Schneider, Falk"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2018-04-23T11:48:54Z"],["dc.date.available","2018-04-23T11:48:54Z"],["dc.date.issued","2017"],["dc.description.abstract","Precise knowledge of the quantum yield is important for many fluorescence–spectroscopic techniques, for example, for Förster resonance energy transfer. However, to measure it for emitters in a complex environment and at low concentrations is far from being trivial. Using a plasmonic nanocavity, we measure the absolute quantum yield value of lipid-conjugated dyes incorporated into a supported lipid bilayer. We show that for both hydrophobic and hydrophilic molecules the quantum yield of dyes inside the lipid bilayer strongly differs from its value in aqueous solution. This finding is of particular importance for all fluorescence–spectroscopic studies involving lipid bilayers, such as protein–protein or protein–lipid interactions in membranes or direct fluorescence–spectroscopic measurements of membrane physical properties."],["dc.identifier.doi","10.1021/acs.jpclett.7b00422"],["dc.identifier.gro","3142101"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13599"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1948-7185"],["dc.title","Quantum Yield Measurements of Fluorophores in Lipid Bilayers Using a Plasmonic Nanocavity"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6836"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry. A, Molecules, spectroscopy, kinetics, environment & general theory"],["dc.bibliographiccitation.lastpage","6841"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Patra, Digambara"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:25Z"],["dc.date.available","2021-03-05T08:58:25Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1021/jp048188m"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80128"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.eissn","1520-5215"],["dc.relation.issn","1089-5639"],["dc.title","Image Analysis of Defocused Single-Molecule Images for Three-Dimensional Molecule Orientation Studies"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","204201"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","The Journal of Chemical Physics"],["dc.bibliographiccitation.volume","148"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Stein, Simon C"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-05-29T09:29:43Z"],["dc.date.available","2020-05-29T09:29:43Z"],["dc.date.issued","2018-05-28"],["dc.description.abstract","Our paper presents the first theoretical and experimental study using single-molecule Metal-Induced Energy Transfer (smMIET) for localizing single fluorescent molecules in three dimensions. Metal-Induced Energy Transfer describes the resonant energy transfer from the excited state of a fluorescent emitter to surface plasmons in a metal nanostructure. This energy transfer is strongly distance-dependent and can be used to localize an emitter along one dimension. We have used Metal-Induced Energy Transfer in the past for localizing fluorescent emitters with nanometer accuracy along the optical axis of a microscope. The combination of smMIET with single-molecule localization based super-resolution microscopy that provides nanometer lateral localization accuracy offers the prospect of achieving isotropic nanometer localization accuracy in all three spatial dimensions. We give a thorough theoretical explanation and analysis of smMIET, describe its experimental requirements, also in its combination with lateral single-molecule localization techniques, and present first proof-of-principle experiments using dye molecules immobilized on top of a silica spacer, and of dye molecules embedded in thin polymer films."],["dc.identifier.doi","10.1063/1.5027074"],["dc.identifier.pmid","29865842"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66012"],["dc.language.iso","en"],["dc.relation.eissn","1089-7690"],["dc.relation.issn","0021-9606"],["dc.title","Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","790330"],["dc.bibliographiccitation.seriesnr","21"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Krämer, B."],["dc.contributor.author","Koberling, F."],["dc.contributor.author","Erdmann, Rainer"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Wahl, M."],["dc.contributor.author","Fore, S."],["dc.contributor.editor","Periasamy, Ammasi"],["dc.contributor.editor","König, Karsten"],["dc.contributor.editor","So, Peter T. C."],["dc.date.accessioned","2018-05-02T15:05:10Z"],["dc.date.available","2018-05-02T15:05:10Z"],["dc.date.issued","2011"],["dc.description.abstract","The combination of simultaneous spectral detection together with Fluorescence Lifetime Imaging (sFLIM) allows collecting the complete information inherent to the fluorescence signal. Their fingerprint of lifetime and spectral properties identify the fluorescent labels unambiguously. Multiple labels can be investigated in parallel and separated from inherent auto-fluorescence of the sample. In addition, spectral FLIM FRET has the prospect to allow simultaneous detection of multiple FRET signals with quantitative analysis of FRET-efficiency and degree of binding. Spectral FLIM measurements generate huge amount of data. Suitable analysis procedures must be found to condense the inherent information to answer the scientific questions in a straightforward way. Different analysis techniques have been evaluated for a diversity of applications as multiplex labeling, quantitative determination of environmental parameters and distance measurements via FLIM FRET. In order to reach highest sensitivity in single photon detection, different detector types are investigated and developed. SPAD arrays equipped with micro-lenses promise superior detection efficiency while the integration of a spectrograph with a PMT array is easier to realize and allows for a higher number of detection channels. High detection speed can be realized through parallel TCSPC channels. In order to overcome the limits of the USB 2.0 interface, new interface solutions have been realized for the multichannel TCSPC unit HydraHarp 400."],["dc.identifier.doi","10.1117/12.873386"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13817"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.publisher","SPIE"],["dc.publisher.place","Washington"],["dc.relation.crisseries","Progress in Biomedical Optics and Imaging"],["dc.relation.doi","10.1117/12.873386"],["dc.relation.eventend","2011-01-25"],["dc.relation.eventlocation","San Francisco, California, United States"],["dc.relation.eventstart","2011-01-23"],["dc.relation.isbn","978-0-8194-8440-6"],["dc.relation.ispartof","Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI"],["dc.relation.ispartofseries","Progress in biomedical optics and imaging 12;21"],["dc.relation.issn","1605-7422"],["dc.relation.issn","1605-7422"],["dc.title","Fast algorithms for the analysis of spectral FLIM data"],["dc.type","conference_paper"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2527"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Optics Letters"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:59:10Z"],["dc.date.available","2021-03-05T08:59:10Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1364/OL.30.002527"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80380"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.eissn","1539-4794"],["dc.relation.issn","0146-9592"],["dc.title","Focusing astigmatic Gaussian beams through optical systems with a high numerical aperture"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","033106"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Review of Scientific Instruments"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Wahl, Michael"],["dc.contributor.author","Rahn, Hans-Jürgen"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Erdmann, Rainer"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:47:05Z"],["dc.date.available","2018-04-23T11:47:05Z"],["dc.date.issued","2007"],["dc.description.abstract","Time-correlated single photon counting is a powerful method for sensitive time-resolved fluorescence measurements down to the single molecule level. The method is based on the precisely timed registration of single photons of a fluorescence signal. Historically, its primary goal was the determination of fluorescence lifetimes upon optical excitation by a short light pulse. This goal is still important today and therefore has a strong influence on instrument design. However, modifications and extensions of the early designs allow for the recovery of much more information from the detected photons and enable entirely new applications. Here, we present a new instrument that captures single photon events on multiple synchronized channels with picosecond resolution and over virtually unlimited time spans. This is achieved by means of crystal-locked time digitizers with high resolution and very short dead time. Subsequent event processing in programmable logic permits classical histogramming as well as time tagging of individual photons and their streaming to the host computer. Through the latter, any algorithms and methods for the analysis of fluorescence dynamics can be implemented either in real time or offline. Instrument test results from single molecule applications will be presented."],["dc.identifier.doi","10.1063/1.2715948"],["dc.identifier.gro","3142178"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13296"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0034-6748"],["dc.title","Dead-time optimized time-correlated photon counting instrument with synchronized, independent timing channels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","433"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","443"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Dertinger, Thomas"],["dc.contributor.author","Pacheco, Victor"],["dc.contributor.author","von der Hocht, Iris"],["dc.contributor.author","Hartmann, Rudolf"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:47:06Z"],["dc.date.available","2018-04-23T11:47:06Z"],["dc.date.issued","2007"],["dc.description.abstract","We present a new method to measure absolute diffusion coefficients at nanomolar concentrations with high precision. Based on a modified fluorescence correlation spectroscopy (FCS)‐setup, this method is improved by introducing an external ruler for measuring the diffusion time by generating two laterally shifted and overlapping laser foci at a fixed and known distance. Data fitting is facilitated by a new two‐parameter model to describe the molecule detection function (MDF). We present a recorded MDF and show the excellent agreement with the fitting model. We measure the diffusion coefficient of the red fluorescent dye Atto655 under various conditions and compare these values with a value achieved by gradient pulsed field NMR (GPF NMR). From these measurements we conclude, that the new measurement scheme is robust against optical and photophysical artefacts which are inherent to standard FCS. With two‐focus‐FCS, the diffusion coefficient of 4.26×10−6 cm2 s−1 for Atto655 in water at 25 °C compares well with the GPF NMR value of 4.28×10−6 cm2 s−1."],["dc.identifier.doi","10.1002/cphc.200600638"],["dc.identifier.gro","3142179"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13297"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1439-4235"],["dc.title","Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]