Enderlein, Jörg

[["dc.bibliographiccitation.artnumber","15542"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Solovyev, Ilya D."],["dc.contributor.author","Gavshina, Alexandra V."],["dc.contributor.author","Katti, Aditya S."],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Vinokurov, Leonid M."],["dc.contributor.author","Lapshin, Grigory D."],["dc.contributor.author","Ivashina, Tatiana V."],["dc.contributor.author","Khrenova, Maria G."],["dc.contributor.author","Kireev, Igor I."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Savitsky, Alexander P."],["dc.date.accessioned","2019-07-09T11:50:56Z"],["dc.date.available","2019-07-09T11:50:56Z"],["dc.date.issued","2018"],["dc.description.abstract","Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeeded, via rational mutagenesis, to obtain variants that formed either tetramers or monomers. We compare two approaches: one is based on the structural similarity between SAASoti and Kaede, which helped us to identify a single point mutation (V127T) at the protein's hydrophobic interface that leads to monomerization. The other is based on a chemical modification of amino groups of SAASoti with succinic anhydride, which converts the protein aggregates into monomers. Mass-spectrometric analysis helped us to identify that the modification of a single ε-amino group of lysine K145 in the strongly charged interface AB was sufficient to convert the protein into its tetrameric form. Furthermore, site-directed mutagenesis was used to generate mutants that proved to be either monomeric or tetrameric, both capable of rapid green-to-red photoconversion. This allows SAASoti to be used as a photoconvertible fluorescent marker for in vivo cell studies."],["dc.identifier.doi","10.1038/s41598-018-33250-z"],["dc.identifier.pmid","30341334"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16025"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59853"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","530"],["dc.title","Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]