Bader, Oliver

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Taverne-Ghadwal, Liliane"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Schulze, Marco H."],["dc.contributor.author","Mbaitolum, Weina Joseph"],["dc.contributor.author","Kersch, Lydia"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Groß, U."],["dc.date.accessioned","2022-04-01T10:00:45Z"],["dc.date.available","2022-04-01T10:00:45Z"],["dc.date.issued","2022"],["dc.description.abstract","Oral candidiasis remains a common problem in HIV-infected individuals, especially in sub-Saharan Africa. Here, we performed the first study in Chad on the prevalence of oral yeasts carriage and oral candidiasis in HIV-positive subjects from southern Chad and analyzed the influence of HAART, CD4 + T-cell numbers, and antimycotics in 589 patients. These patients were recruited from a specialized medical center for HIV patients in Sarh and from a rural medical health dispensary in the vicinity, including a total of 384 HIV-positive and 205 HIV-negative individuals. Yeasts obtained from oral specimen were identified by MALDI-TOF MS and their antifungal susceptibility profiles determined. The overall prevalence of yeast colonization and symptomatic oral candidiasis in HIV-infected patients was 25.1%. The prevalence of oral candidiasis was higher in untreated than in HAART-treated HIV-positive patients (16% vs. 2%; p  < 0.01). Oral candidiasis was furthermore associated with high fungal burdens of Candida albicans and a CD4 + T-cell number <200/μl. A shift toward non -albicans Candida species was observed under nucleoside-based HAART therapy. Azole antifungal drug resistance was only observed for the intrinsically resistant species Candida krusei and Candida glabrata . Prevalence of oral candidiasis in the studied area was very low. The species distribution was similar to other countries around the world, with C. albicans being dominant. Candida dubliniensis was not isolated. Nucleoside-based HAART therapy significantly reduced oral colonization as well as occurrence of oral candidiasis caused by C. albicans and led to a species shift toward non- albicans species. Antifungal resistance was not yet a concern in Chad."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3389/fmicb.2022.844069"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105503"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Epidemiology and Prevalence of Oral Candidiasis in HIV Patients From Chad in the Post-HAART Era"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e70425"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Morais, Janne K. S."],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Oliveira, Jose Tadeu A."],["dc.contributor.author","Arantes, Mariana R."],["dc.contributor.author","Gomes, Valdirene M."],["dc.contributor.author","Da Cunha, Maura"],["dc.contributor.author","Oliveira, Hermogenes D."],["dc.contributor.author","Sousa, Daniele O. B."],["dc.contributor.author","Lourencao, Andre L."],["dc.contributor.author","Vasconcelos, Ilka M."],["dc.date.accessioned","2018-11-07T09:22:19Z"],["dc.date.available","2018-11-07T09:22:19Z"],["dc.date.issued","2013"],["dc.description.abstract","Soybean toxin (SBTX) is an antifungal protein from soybeans with broad inhibitory activity against the growth and filamentation of many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina and Cercospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute to the design of novel antifungal drugs and/or the development of transgenic plants resistant to pathogens. To this end, the polymorphic yeast C. albicans was chosen as a model organism and changes in the gene expression profile of strain SC5314 upon exposure to SBTX were examined. Genes that were differentially regulated in the presence of SBTX were involved in glucose transport and starvation-associated stress responses as well as in the control of both the induction and repression of C. albicans hyphal formation. Transmission electron microscopy showed that C. albicans cells exposed to SBTX displayed severe signs of starvation and were heavily granulated. Our data were indicative of C. albicans cell starvation despite sufficient nutrient availability in the medium; therefore, it can be speculated that SBTX blocks nutrient uptake systems. Because neither the starvation signal nor the alkaline response pathway lead to the induction of hyphae, we hypothesise that conflicting signals are transmitted to the complex regulatory network controlling morphogenesis, eventually preventing the filamentation signal from reaching a significant threshold."],["dc.identifier.doi","10.1371/journal.pone.0070425"],["dc.identifier.isi","000322132100094"],["dc.identifier.pmid","23894655"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29314"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","172"],["dc.bibliographiccitation.journal","BMC Pregnancy and Childbirth"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Voelker, Fabian M."],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Uy, Angela"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T10:22:50Z"],["dc.date.available","2018-11-07T10:22:50Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Although infectious diseases still account for a high burden of morbidity and mortality in sub-Saharan Africa, simultaneous investigations on multiple infections affecting maternal and child health are missing. Methods: We conducted a cross-sectional, single-centre pilot study in a rural area of Ghana to assess the infectiological profile during pregnancy. Screening of 180 expectant mothers was done by vaginal swabs and serology to detect the most common pregnancy-relevant infections. They were also interviewed for potential risk factors, outcome of previous pregnancies, and socio-economic aspects. Results: We found a high prevalence of infections caused by hepatitis B virus (16.7% HBs antigen positive). In contrast, infections caused by hepatitis C virus (1.1% anti-HCV) and HIV (0.6%) were rare. Maternal malaria was frequent (10.6%), despite increasing acceptance of intermittent preventive treatment during pregnancy (IPTp). Group B streptococci were present in 10.6% of all pregnant women. Absence of antibodies against varicella zoster virus in 43.2%, Toxoplasma gondii in 26.8%, parvovirus B19 in 20.0%, and rubella virus in 15.7% makes a significant proportion of pregnant women susceptible for acquiring primary infections. Whereas all study participants had specific IgG antibodies against human cytomegalovirus, infections with Listeria, Brucella, or Neisseria gonorrhoeae as well as active syphilis were absent. Conclusions: Our pilot study in a rural community in Ghana indicates an urgent need for action in dealing at least with high-prevalent pregnancy-relevant infections, such as hepatitis B, malaria and those caused by group B streptococci. In addition, the resulting prevalence rates of various other infections may offer guidance for health officials to prioritize possible future intervention schemes."],["dc.identifier.doi","10.1186/s12884-017-1351-3"],["dc.identifier.isi","000402791800001"],["dc.identifier.pmid","28583150"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42347"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2393"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Prevalence of pregnancy-relevant infections in a rural setting of Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Cellular and Infection Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Bernhard, Mareike"],["dc.contributor.author","Worasilchai, Navaporn"],["dc.contributor.author","Kangogo, Mourine"],["dc.contributor.author","Bii, Christine"],["dc.contributor.author","Trzaska, Wioleta J."],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Chindamporn, Ariya"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2021-06-01T09:42:23Z"],["dc.date.available","2021-06-01T09:42:23Z"],["dc.date.issued","2021"],["dc.description.abstract","Yeasts of the Cryptococcus neoformans / gattii species complexes are human pathogens mostly in immune compromised individuals, and can cause infections from dermal lesions to fungal meningitis. Differences in virulence and antifungal drug susceptibility of species in these complexes indicate the value of full differentiation to species level in diagnostic procedures. MALDI-TOF MS has been reported to sufficiently discriminate these species. Here, we sought to re-evaluate sample pre-processing procedures and create a set of publicly available references for use with the MALDI Biotyper system. Peak content using four different pre-processing protocols was assessed, and database entries for 13 reference strains created. These were evaluated against a collection of 153 clinical isolates, typed by conventional means. The use of decapsulating protocols or mechanical disruption did not sufficiently increase the information content to justify the extra hands-on-time. Using the set of 13 reference entries created with the standard formic acid extraction, we were able to correctly classify 143/153 (93.5%) of our test isolates. The majority of the remaining ten isolates still gave correct top matches; only two isolates did not give reproducible identifications. This indicates that the log score cut-off can be lowered also in this context. Ease to identify cryptococcal isolates to the species level is improved by the workflow evaluated here. The database references are freely available from https://github.com/oliverbader/BioTyper-libraries for incorporation into local diagnostic systems."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3389/fcimb.2021.634382"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85236"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2235-2988"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","CryptoType – Public Datasets for MALDI-TOF-MS Based Differentiation of Cryptococcus neoformans/gattii Complexes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","501"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Vaz, Catarina"],["dc.contributor.author","Pitarch, Aida"],["dc.contributor.author","Gómez-Molero, Emilia"],["dc.contributor.author","Amador-García, Ahinara"],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Monteoliva, Lucía"],["dc.contributor.author","Gil, Concha"],["dc.date.accessioned","2021-09-01T06:43:05Z"],["dc.date.available","2021-09-01T06:43:05Z"],["dc.date.issued","2021"],["dc.description.abstract","Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests."],["dc.description.abstract","Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests."],["dc.description.sponsorship","European Commission"],["dc.description.sponsorship","Ministerio de Economía, Industria y Competitividad, Gobierno de España"],["dc.description.sponsorship","Comunidad de Madrid"],["dc.description.sponsorship","Instituto de Salud Carlos III"],["dc.description.sponsorship","European Regional Development Fund"],["dc.identifier.doi","10.3390/jof7070501"],["dc.identifier.pii","jof7070501"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89215"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.publisher","MDPI"],["dc.relation.eissn","2309-608X"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2087"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Joppe, Felix M."],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Kusumawati, R. Lia"],["dc.contributor.author","Berger, Fabian K."],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-09-24T08:07:22Z"],["dc.date.available","2019-09-24T08:07:22Z"],["dc.date.issued","2019"],["dc.description.abstract","Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. During the past decade, hypervirulent PCR-ribotypes (RT) e.g., RT027 or RT176 emerged rapidly all over the world, associated with both, increased severity and mortality rates. It is thus of great importance to identify epidemic strains such as RT027 and RT176 as fast as possible. While commonly used diagnostic methods, e.g., multilocus sequence typing (MLST) or PCR-ribotyping, are time-consuming, proteotyping offers a fast, inexpensive, and reliable alternative solution. In this study, we established a MALDI-TOF-based typing scheme for C. difficile. A total of 109 ribotyped strains representative for five MLST clades were analyzed by MALDI-TOF. MLST, based on whole genome sequences, and PCR-ribotyping were used as reference methods. Isoforms of MS-detectable biomarkers, typically ribosomal proteins, were related with the deduced amino acid sequences and added to the C. difficile proteotyping scheme. In total, we were able to associate nine biomarkers with their encoding genes and include them in our proteotyping scheme. The discriminatory capacity of the C. difficile proteotyping scheme was mainly based on isoforms of L28-M (2 main isoforms), L35-M (4 main isoforms), and S20-M (2 main isoforms) giving rise to at least 16 proteotyping-derived types. In our test population, five of these 16 proteotyping-derived types were detected. These five proteotyping-derived types did not correspond exactly to the included five MLST-based C. difficile clades, nevertheless the subtyping depth of both methods was equivalent. Most importantly, proteotyping-derived clade B contained only isolates of the hypervirulent RT027 and RT176. Proteotyping is a stable and easy-to-perform intraspecies typing method and a promising alternative to currently used molecular techniques. It is possible to distinguish the group of RT027 and RT176 isolates from non-RT027/non-RT176 isolates using proteotyping, providing a valuable diagnostic tool."],["dc.identifier.doi","10.3389/fmicb.2019.02087"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16398"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62451"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","3862"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Nanomaterials"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Kreutz, Marietta"],["dc.contributor.author","Kreutz, Christian"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Tauböck, Tobias T."],["dc.contributor.author","Burrer, Phoebe"],["dc.contributor.author","Noll, Christine"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Rohland, Bianca"],["dc.contributor.author","Wiegand, Annette"],["dc.contributor.author","Rizk, Marta"],["dc.date.accessioned","2022-12-01T08:31:45Z"],["dc.date.available","2022-12-01T08:31:45Z"],["dc.date.issued","2022-11-01"],["dc.description.abstract","The aim of the study was to examine the applicability of bioactive and antibacterial nanoparticles to an experimental adhesive. The adhesive (60 wt% BisGMA, 15 wt% TEGDMA, 25 wt% HEMA) was mixed with combinations of 5 wt% methacryl-functionalized polyhedral oligomeric silsesquioxane (MA-POSS) and one kind of bioactive/antibacterial nanoparticles: 1 wt% core-shell silica-silver nanoparticle (SiO2@Ag), 1 wt% bioactive glass with bismuth (BAG-Bi) or 1 wt% calcium phosphate (CAP). Pure adhesive served as control. The physicochemical (degree of conversion (DC), linear shrinkage (LS), shear and complex viscosity, water sorption (WS), sol fraction (SF)), biological (antimicrobial effect) and bioactive (mineral precipitation) properties were investigated. DC and LS remained unchanged. The combination of BAG-Bi/MA-POSS resulted in a significantly increased WS and SF compared to control. In addition, the combination of CAP/MA-POSS slightly increased the shear viscosity of the adhesive. The addition of the nanoparticles did not influence the antimicrobial effects compared to the pure adhesive. Improved mineral inducing capacity could be detected in all nanoparticle combinations. The combination of bioactive and/or antibacterial nanoparticles showed improved mineral inducing capacity, but no antibacterial properties. The material properties were not or only slightly affected."],["dc.identifier.doi","10.3390/nano12213862"],["dc.identifier.pii","nano12213862"],["dc.identifier.pmcid","PMC9694625"],["dc.identifier.pmid","36364638"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118257"],["dc.identifier.url","https://www.mdpi.com/2079-4991/12/21/3862"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","2079-4991"],["dc.relation.orgunit","Poliklinik für Präventive Zahnmedizin, Parodontologie und Kariologie"],["dc.rights","CC BY 4.0"],["dc.subject.gro","bioactive nanoparticles"],["dc.subject.gro","antimicrobial nanoparticles"],["dc.subject.gro","dental adhesive"],["dc.subject.gro","POSS"],["dc.subject.gro","SiO2@Ag"],["dc.subject.gro","calcium phosphate"],["dc.subject.gro","bioactive glass"],["dc.title","Effect of Bioactive and Antimicrobial Nanoparticles on Properties and Applicability of Dental Adhesives"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e52218"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Schwarz, Alexander"],["dc.contributor.author","Kraneveld, Eefje A."],["dc.contributor.author","Tangwattanchuleeporn, Marut"],["dc.contributor.author","Schmidt, Pia"],["dc.contributor.author","Jacobsen, Mette D."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","de Groot, Piet W. J."],["dc.contributor.author","Weig, Michael S."],["dc.date.accessioned","2018-11-07T09:02:13Z"],["dc.date.available","2018-11-07T09:02:13Z"],["dc.date.issued","2012"],["dc.description.abstract","Genomic plasticity is a mechanism for adaptation to environmental cues such as host responses and antifungal drug pressure in many fungi including the human pathogenic yeast Candida glabrata. In this study we evaluated the phenotypic and genotypic stability of the world-wide used C. glabrata reference strain CBS138/ ATCC2001 under laboratory conditions. A set of ten lineages of this wild type strain and genetically modified progenies were obtained from different scientific laboratories, and analyzed for genotypic and phenotypic alterations. Even though the derivates were indistinguishable by multi locus sequence typing, different phenotypic groups that correlated with specific karyotypic changes were observed. In addition, modifications in the adherence capacity to plastic surface emerged that were shown to correlate with quantitative changes in adhesin gene expression rather than subtelomeric gene loss or differences in the number of macrosatellite repeats within adhesin genes. These results confirm the genomic plasticity of C. glabrata and show that chromosomal aberrations and functional adaptations may occur not only during infection and under antimicrobial therapy, but also under laboratory conditions without extreme selective pressures. These alterations can significantly affect phenotypic properties such as cell surface attributes including adhesion and the cell wall carbohydrate composition and therefore, if unnoticed, may adulterate the outcome of genetic studies."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1371/journal.pone.0052218"],["dc.identifier.isi","000312794500117"],["dc.identifier.pmid","23284942"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8472"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24630"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Gross Karyotypic and Phenotypic Alterations among Different Progenies of the Candida glabrata CBS138/ATCC2001 Reference Strain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1088"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Goldschmidt, Anne-Marie"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Schuldes, Jörg"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Stingl, Kerstin"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Lingner, Thomas"],["dc.date.accessioned","2018-11-07T09:47:24Z"],["dc.date.available","2018-11-07T09:47:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to Campylobacter jejuni, about 5 % of Campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by DpnI cutting specifically 5'-G(m)ATC-3' motifs. This indicates significant differences in DNA methylation between both microbial species. The goal of the study was to analyze the methylome of a C. coli strain susceptible to DpnI digestion, to identify its methylation motifs and restriction modification systems (RM-systems), and compare them to related organisms like C. jejuni and Helicobacter pylori. Results: Using one SMRT cell and the PacBio RS sequencing technology followed by PacBio Modification and Motif Analysis the complete genome of the DpnI susceptible strain C. coli BfR-CA-9557 was sequenced to 500-fold coverage and assembled into a single contig of 1.7 Mbp. The genome contains a CJIE1-like element prophage and is phylogenetically closer to C. coli clade 1 isolates than clade 3. 45,881 6-methylated adenines (ca. 2.7 % of genome positions) that are predominantly arranged in eight different methylation motifs and 1,788 4-methylated cytosines (ca. 0.1 %) have been detected. Only two of these motifs correspond to known restriction modification motifs. Characteristic for this methylome was the very high fraction of methylation of motifs with mostly above 99 %. Conclusions: Only five dominant methylation motifs have been identified in C. jejuni, which have been associated with known RM-systems. C. coli BFR-CA-9557 shares one (RAATTY) of these, but four ORFs could be assigned to putative Type I RM-systems, seven ORFs to Type II RM-systems and three ORFs to Type IV RM-systems. In accordance with DpnI prescreening RM-system IIP, methylation of GATC motifs was detected in C. coli BfR-CA-9557. A homologous IIP RM-system has been described for H. pylori. The remaining methylation motifs are specific for C. coli BfR-CA-9557 and have been neither detected in C. jejuni nor in H. pylori. The results of this study give us new insights into epigenetics of Campylobacteraceae and provide the groundwork to resolve the function of RM-systems in C. coli."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1186/s12864-015-2317-3"],["dc.identifier.isi","000367061700011"],["dc.identifier.pmid","26689587"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35106"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Dohmen, Maria"],["dc.contributor.author","Walter, Sebastian G."],["dc.contributor.author","Noll, Christine"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2021-04-14T08:27:19Z"],["dc.date.available","2021-04-14T08:27:19Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s41467-020-19718-5"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17801"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82239"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Remote near infrared identification of pathogens with multiplexed nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]