Ninković, Milena

[["dc.bibliographiccitation.firstpage","1932"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","1940"],["dc.bibliographiccitation.volume","94"],["dc.contributor.author","Dučić, Tanja"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Martínez-Rovira, Immaculada"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Dimitrijević, Dragoljub"],["dc.contributor.author","Jover Mañas, Gabriel Vicent"],["dc.contributor.author","Vaccari, Lisa"],["dc.contributor.author","Birarda, Giovanni"],["dc.contributor.author","Yousef, Ibraheem"],["dc.date.accessioned","2022-02-01T10:32:13Z"],["dc.date.available","2022-02-01T10:32:13Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1021/acs.analchem.1c02076"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99037"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Translational Stroke Research"],["dc.contributor.author","Döring, Katja"],["dc.contributor.author","Schroeder, Henning"],["dc.contributor.author","Fischer, André"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Mielke, Dorothee"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Malinova, Vesna"],["dc.date.accessioned","2022-02-01T10:31:59Z"],["dc.date.available","2022-02-01T10:31:59Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Cerebral vasospasm is a highly investigated phenomenon in neurovascular research. Experimental vasospasm models are irreplaceable for the evaluation of new antivasospastic drugs. In this study, we assessed the reliability of in vivo vasospasm induction by ultrasound application in the chicken chorioallantoic membrane (CAM) model. After incubation of fertilized chicken eggs for four days, a fenestration was performed to enable examination of the CAM vessels. On the thirteenth day, continuous-wave ultrasound (3 MHz, 1 W/cm 2 ) was applied on the CAM vessels for 60 s. The ultrasound effect on the vessels was recorded by life imaging (5-MP HD-microscope camera, Leica®). The induced vessel diameter changes were evaluated in a defined time interval of 20 min using a Fiji macro. The vessel diameter before and after sonication was measured and the relative diameter reduction was determined. A first reduction of vessel diameter was observed after three minutes with an average vessel-diameter decrease to 77%. The maximum reduction in vessel diameter was reached eight minutes after sonication with an average vessel diameter decrease to 57% (mean relative diameter reduction of 43%, range 44–61%), ANOVA, p  = 0.0002. The vasospasm persisted for all 20 recorded minutes post induction. Vasospasm can be reliably induced by short application of 3 MHz-ultrasound to the CAM vessels. This might be a suitable in vivo model for the evaluation of drug effects on vasospasm in an experimental setting as intermediary in the transition process from in vitro to in vivo assessment using animal models."],["dc.description.abstract","Abstract Cerebral vasospasm is a highly investigated phenomenon in neurovascular research. Experimental vasospasm models are irreplaceable for the evaluation of new antivasospastic drugs. In this study, we assessed the reliability of in vivo vasospasm induction by ultrasound application in the chicken chorioallantoic membrane (CAM) model. After incubation of fertilized chicken eggs for four days, a fenestration was performed to enable examination of the CAM vessels. On the thirteenth day, continuous-wave ultrasound (3 MHz, 1 W/cm 2 ) was applied on the CAM vessels for 60 s. The ultrasound effect on the vessels was recorded by life imaging (5-MP HD-microscope camera, Leica®). The induced vessel diameter changes were evaluated in a defined time interval of 20 min using a Fiji macro. The vessel diameter before and after sonication was measured and the relative diameter reduction was determined. A first reduction of vessel diameter was observed after three minutes with an average vessel-diameter decrease to 77%. The maximum reduction in vessel diameter was reached eight minutes after sonication with an average vessel diameter decrease to 57% (mean relative diameter reduction of 43%, range 44–61%), ANOVA, p  = 0.0002. The vasospasm persisted for all 20 recorded minutes post induction. Vasospasm can be reliably induced by short application of 3 MHz-ultrasound to the CAM vessels. This might be a suitable in vivo model for the evaluation of drug effects on vasospasm in an experimental setting as intermediary in the transition process from in vitro to in vivo assessment using animal models."],["dc.identifier.doi","10.1007/s12975-021-00960-y"],["dc.identifier.pii","960"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98997"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1868-601X"],["dc.relation.issn","1868-4483"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","In Vivo Vasospasm Induction by Ultrasound Application in the Chicken Chorioallantoic Membrane Model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1037"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Acta Neurochirurgica"],["dc.bibliographiccitation.lastpage","1045"],["dc.bibliographiccitation.volume","161"],["dc.contributor.author","Fiss, Ingo"],["dc.contributor.author","Hussein, Abdelhalim"],["dc.contributor.author","Barrantes-Freer, Alonso"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Hernandez-Duran, Silvia"],["dc.contributor.author","Wolfert, Christina"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Mielke, Dorothee"],["dc.contributor.author","Schatlo, Bawarjan"],["dc.date.accessioned","2020-12-10T14:10:53Z"],["dc.date.available","2020-12-10T14:10:53Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00701-019-03842-3"],["dc.identifier.eissn","0942-0940"],["dc.identifier.issn","0001-6268"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70910"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Cerebral metastases: do size, peritumoral edema, or multiplicity predict infiltration into brain parenchyma?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","207"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","214"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","SACHKOVA, ALEKSANDRA"],["dc.contributor.author","SPERLING, SWETLANA"],["dc.contributor.author","MIELKE, DOROTHEE"],["dc.contributor.author","SCHATLO, BAWARJAN"],["dc.contributor.author","ROHDE, VEIT"],["dc.contributor.author","NINKOVIC, MILENA"],["dc.date.accessioned","2020-12-10T18:43:04Z"],["dc.date.available","2020-12-10T18:43:04Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.21873/anticanres.13099"],["dc.identifier.eissn","1791-7530"],["dc.identifier.issn","0250-7005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78182"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Combined Applications of Repurposed Drugs and Their Detrimental Effects on Glioblastoma Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S1773224721005141"],["dc.bibliographiccitation.firstpage","102834"],["dc.bibliographiccitation.journal","Journal of Drug Delivery Science and Technology"],["dc.bibliographiccitation.volume","66"],["dc.contributor.author","Döring, Katja"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Gasimov, Turab"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Streit, Frank"],["dc.contributor.author","Binder, Lutz"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Malinova, Vesna"],["dc.date.accessioned","2021-12-01T09:23:36Z"],["dc.date.available","2021-12-01T09:23:36Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.jddst.2021.102834"],["dc.identifier.pii","S1773224721005141"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94703"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.issn","1773-2247"],["dc.title","Ultrasound-induced release of nimodipine from drug-loaded block copolymers: In vitro analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","96697"],["dc.bibliographiccitation.firstpage","96697"],["dc.bibliographiccitation.issue","57"],["dc.bibliographiccitation.journal","Oncotarget"],["dc.bibliographiccitation.lastpage","96709"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Aung, Thiha"],["dc.contributor.author","Martin, Sabine"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Ninkovic, Milena"],["dc.date.accessioned","2018-10-09T07:07:52Z"],["dc.date.available","2018-10-09T07:07:52Z"],["dc.date.issued","2017-11-14"],["dc.description.abstract","A small subpopulation of tumor stem-like cells has the capacity to initiate tumors and mediate radio- and chemoresistance in diverse cancers hence also in glioblastoma (GBM). It has been reported that this capacity of tumor initiation in the brain is mainly dependent on the body's nutrient supply. This population of so-called brain tumor initiating or brain tumor stem-like cells (BTSCs) is able to extract nutrients like glucose with a higher affinity. Riluzole, a drug approved for treating amyotrophic lateral sclerosis (ALS), was reported to possess anticancer properties, affecting the glutamate metabolism. We report that riluzole treatment inhibits the growth of brain tumor stem-like cells enriched cultures isolated from two human glioblastomas. The effects of riluzole on these cells were associated with an inhibition of a poor prognostic indicator: glucose transporter 3 (GLUT3). A decrease in GLUT3 is associated with a decrease in the p-Akt/HIF1α pathway. Further, downregulation of the DNA (Cytosine-5-)-methyltransferase 1 (DNMT1) gene that causes hypermethylation of various tumor-suppressor genes and leads to a poor prognosis in GBM, was detected. Two hallmarks of cancer cells-proliferation and cell death-were positively influenced by riluzole treatment. Finally, we observed that riluzole reduced the tumor growth in in vivo CAM assay, suggesting it could be a possible synergistic drug for the treatment of glioblastoma."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.fs.pkfprnr","86427"],["dc.identifier.doi","10.18632/oncotarget.18043"],["dc.identifier.fs","633522"],["dc.identifier.pmid","29228563"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14509"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15891"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1949-2553"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Riluzole: a potential therapeutic intervention in human brain tumor stem-like cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Translational Stroke Research"],["dc.contributor.author","Döring, Katja"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Schroeder, Henning"],["dc.contributor.author","Fischer, André"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Streit, Frank"],["dc.contributor.author","Binder, Lutz"],["dc.contributor.author","Mielke, Dorothee"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Malinova, Vesna"],["dc.date.accessioned","2022-02-01T10:31:59Z"],["dc.date.available","2022-02-01T10:31:59Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Nimodipine prevents cerebral vasospasm and improves functional outcome after aneurysmal subarachnoid hemorrhage (aSAH). The beneficial effect is limited by low oral bioavailability of nimodipine, which resulted in an increasing use of nanocarriers with sustained intrathecal drug release in order to overcome this limitation. However, this approach facilitates only a continuous and not an on-demand nimodipine release during the peak time of vasospasm development. In this study, we aimed to assess the concept of controlled drug release from nimodipine-loaded copolymers by ultrasound application in the chicken chorioallantoic membrane (CAM) model. Nimodipine-loaded copolymers were produced with the direct dissolution method. Vasospasm of the CAM vessels was induced by means of ultrasound (Physiomed, continuous wave, 3 MHz, 1.0 W/cm 2 ). The ultrasound-mediated nimodipine release (Physiomed, continuous wave, 1 MHz, 1.7 W/cm 2 ) and its effect on the CAM vessels were evaluated. Measurements of vessel diameter before and after ultrasound-induced nimodipine release were performed using ImageJ. The CAM model could be successfully carried out in all 25 eggs. After vasospasm induction and before drug release, the mean vessel diameter was at 57% (range 44–61%) compared to the baseline diameter (set at 100%). After ultrasound-induced drug release, the mean vessel diameter of spastic vessels increased again to 89% (range 83–91%) of their baseline diameter, which was significant ( p  = 0.0002). We were able to provide a proof of concept for in vivo vasospasm induction by ultrasound application in the CAM model and subsequent resolution by ultrasound-mediated nimodipine release from nanocarriers. This concept merits further evaluation in a rat SAH model. Graphical abstract"],["dc.description.abstract","Abstract Nimodipine prevents cerebral vasospasm and improves functional outcome after aneurysmal subarachnoid hemorrhage (aSAH). The beneficial effect is limited by low oral bioavailability of nimodipine, which resulted in an increasing use of nanocarriers with sustained intrathecal drug release in order to overcome this limitation. However, this approach facilitates only a continuous and not an on-demand nimodipine release during the peak time of vasospasm development. In this study, we aimed to assess the concept of controlled drug release from nimodipine-loaded copolymers by ultrasound application in the chicken chorioallantoic membrane (CAM) model. Nimodipine-loaded copolymers were produced with the direct dissolution method. Vasospasm of the CAM vessels was induced by means of ultrasound (Physiomed, continuous wave, 3 MHz, 1.0 W/cm 2 ). The ultrasound-mediated nimodipine release (Physiomed, continuous wave, 1 MHz, 1.7 W/cm 2 ) and its effect on the CAM vessels were evaluated. Measurements of vessel diameter before and after ultrasound-induced nimodipine release were performed using ImageJ. The CAM model could be successfully carried out in all 25 eggs. After vasospasm induction and before drug release, the mean vessel diameter was at 57% (range 44–61%) compared to the baseline diameter (set at 100%). After ultrasound-induced drug release, the mean vessel diameter of spastic vessels increased again to 89% (range 83–91%) of their baseline diameter, which was significant ( p  = 0.0002). We were able to provide a proof of concept for in vivo vasospasm induction by ultrasound application in the CAM model and subsequent resolution by ultrasound-mediated nimodipine release from nanocarriers. This concept merits further evaluation in a rat SAH model. Graphical abstract"],["dc.identifier.doi","10.1007/s12975-021-00979-1"],["dc.identifier.pii","979"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98998"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1868-601X"],["dc.relation.issn","1868-4483"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Ultrasound-Induced Release of Nimodipine from Drug-Loaded Block Copolymer Micelles: In Vivo Analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]