Heilmann, Ingo

[["dc.bibliographiccitation.firstpage","453"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","The Plant Journal"],["dc.bibliographiccitation.lastpage","468"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Hempel, Franziska"],["dc.contributor.author","Jin, X. U."],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T08:59:38Z"],["dc.date.available","2018-11-07T08:59:38Z"],["dc.date.issued","2011"],["dc.description.abstract","P>The regulation of pollen tube growth by the phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) is not well understood. The Arabidopsis genome encodes two type A phosphatidylinositol-4-phosphate (PI4P) 5-kinases, PIP5K10 and PIP5K11, which are exclusively expressed in pollen and produce PtdIns(4,5)P(2)in vitro. Fluorescence-tagged PIP5K10 and PIP5K11 localized to lateral subapical plasma membrane microdomains in tobacco pollen tubes in a pattern closely resembling the distribution of PtdIns(4,5)P(2,) with the exception of notably weaker association at the extreme apex. Overexpression of PIP5K10 or PIP5K11 in tobacco pollen tubes resulted in severe tip swelling and altered actin fine structure similar to that reported for overexpression of tobacco Nt-Rac5, a monomeric GTPase known to regulate the actin cytoskeleton. Increased sensitivity of Arabidopsis pip5k10 pip5k11 double mutant pollen tubes to Latrunculin B (LatB) further supports a role for type A PI4P 5-kinases in controlling the actin cytoskeleton. Despite the disruption of both its type A PI4P 5-kinases, the pip5k10 pip5k11 double mutant was fertile, indicating that one of the remaining type B PI4P 5-kinase isoforms might be functionally redundant with PIP5K10 and PIP5K11. Antagonistic effects of PIP5K11 and the Nt-Rac5-specific guanine nucleotide dissociation inhibitor, Nt-RhoGDI2, on tip swelling observed in coexpression-titration experiments indicate a link between PtdIns(4,5)P(2) and Rac-signaling in pollen tubes. The data suggest that type A PI4P 5-kinases influence the actin cytoskeleton in pollen tubes in part by counteracting Nt-RhoGDI2, possibly contributing to the control of the pool of plasma membrane-associated Nt-Rac5."],["dc.description.sponsorship","German Research Foundation (DFG) [He3424/1]"],["dc.identifier.doi","10.1111/j.1365-313X.2010.04435.x"],["dc.identifier.isi","000286619000011"],["dc.identifier.pmid","21265898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23950"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0960-7412"],["dc.title","Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","249"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Molecular Plant"],["dc.bibliographiccitation.lastpage","261"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Koenig, Sabine"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Grzeganek, Peter"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T11:17:53Z"],["dc.date.available","2018-11-07T11:17:53Z"],["dc.date.issued","2008"],["dc.description.abstract","Various biochemical signals are implicated in Arabidopsis wound signalling, including jasmonic acid (JA), salicylic acid, auxin, and Ca2+. Here, we report on cross-talk of phytohormones with phosphoinositide signals not previously implicated in plant wound responses. Within 30 min of mechanical wounding of Arabidopsis rosette-leaves, the levels of the lipid-derived soluble inositolpolyphosphate, inositol 1,4,5-trisphosphate (InsP(3)), increased four to five-fold. Concomitantly, the precursor lipids, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol transiently depleted, followed by re-synthesis after 30-60 min of stimulation. Increased InsP(3) levels with wounding coincided with JA increases over the first hours of stimulation. In dde2-2-mutant plants deficient in JA biosynthesis, no InsP(3) increase was observed upon wounding, indicating that JA was required for InsP(3) formation, and InsP(3) levels increased in wild-type plants challenged with sorbitol, increasing endogenous JA levels. In InsP 5-ptase plants with attenuated phosphoinositide signalling, the induction of wounding-inducible genes was diminished compared with wildtype plants, suggesting a role for phosphoinositide signalling in mediating plant wound responses. The gene-expression patterns suggest that phosphoinositides contribute to both JA-dependent and JA-independent aspects of wound signalling. Weight gain of Plutella xylostella caterpillars feeding on InsP 5-ptase plants was increased compared with that of caterpillars feeding on wild-type plants. The ecophysiological relevance of phosphoinositide signals in plant defense responses to herbivory is discussed in light of recent findings of inositolpolyphosphate involvement in phytohormone-receptor function."],["dc.description.sponsorship","German Research Foundation [He3424/1-3]"],["dc.identifier.doi","10.1093/mp/ssm028"],["dc.identifier.isi","000259103700006"],["dc.identifier.pmid","19825537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54919"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1674-2052"],["dc.title","Phosphoinositide and inositolpolyphosphate signalling in defense responses of Arabidopsis thaliana challenged by mechanical wounding"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","837"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Plant Cell & Environment"],["dc.bibliographiccitation.lastpage","850"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Bargmann, Bastiaan O. R."],["dc.contributor.author","Laxalt, Ana M."],["dc.contributor.author","Ter Riet, Bas"],["dc.contributor.author","Testerink, Christa"],["dc.contributor.author","Merquiol, Emmanuelle"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Leon-Reyes, Antonio"],["dc.contributor.author","Pieterse, Corne M. J."],["dc.contributor.author","Haring, Michel A."],["dc.contributor.author","Heilmann, Ingo"],["dc.contributor.author","Bartels, Dorothea"],["dc.contributor.author","Munnik, Teun"],["dc.date.accessioned","2018-11-07T08:28:32Z"],["dc.date.available","2018-11-07T08:28:32Z"],["dc.date.issued","2009"],["dc.description.abstract","Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis (Arabidopsis thaliana) AtPLD alpha 1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using (32)P(i)-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD-silenced tomato cell-suspension cultures. pld alpha 1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a pld alpha 1/delta double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly (Pieris rapae) grew equally well on wild-type and the pld knock-out mutants."],["dc.identifier.doi","10.1111/j.1365-3040.2009.01962.x"],["dc.identifier.isi","000266601600007"],["dc.identifier.pmid","19220780"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16442"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0140-7791"],["dc.title","Reassessing the role of phospholipase D in the Arabidopsis wounding response"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1958"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The FASEB Journal"],["dc.bibliographiccitation.lastpage","1967"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Koenig, Sabine"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T11:00:59Z"],["dc.date.available","2018-11-07T11:00:59Z"],["dc.date.issued","2007"],["dc.description.abstract","Function and development of eukaryotic cells require tight control of diverse physiological processes. Numerous cellular processes are regulated by polyphosphoinositides, which interact with protein partners or mediate release of the second messenger, inositol 1,4,5-trisphosphate (InsP3). Emerging evidence suggests that different regulatory or signaling functions of polyphosphoinositides may be orchestrated by the establishment of distinct subcellular pools; the principles underlying pool-formation are, however, not understood. Arabidopsis plants exhibit transient increases in polyphosphoinositides with hyperosmotic stress, providing a model for comparing constitutive and stress-inducible polyphosphoinositide pools. Using a combination of thin-layer-chromatography and gaschromatography, phospholipids from stressed and nonstressed Arabidopsis plants were analyzed for their associated fatty acids. Under nonstress conditions structural phospholipids and phosphatidylinositol contained 50-70 mol% polyunsaturated fatty acids (PUFA), whereas polyphosphoinositides were more saturated (10-20 mol% PUFA). With hyperosmotic stress polyphosphoinositides with up to 70 mol% PUFA were formed that differed from constitutive species and coincided with a transient loss in unsaturated phosphatidylinositol. The patterns indicate inducible turnover of an unsaturated phosphatidylinositol pool, which accumulates under standard conditions and is primed for phosphorylation on stimulation. Metabolic analysis of wild-type and transgenic plants disturbed in phosphoinositide metabolism suggests that, in contrast to saturated species, unsaturated polyphosphoinositides are channeled toward InsP3-production."],["dc.identifier.doi","10.1096/fj.06-7887com"],["dc.identifier.isi","000247500300006"],["dc.identifier.pmid","17327357"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51049"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Federation Amer Soc Exp Biol"],["dc.relation.issn","0892-6638"],["dc.title","Stress-inducible and constitutive phosphoinositide pools have distinctive fatty acid patterns in Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","949"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The Plant Journal"],["dc.bibliographiccitation.lastpage","957"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Gatz, Christiane"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T08:59:06Z"],["dc.date.available","2018-11-07T08:59:06Z"],["dc.date.issued","2011"],["dc.description.abstract","Plant responses to wounding are part of their defense responses against insects, and are tightly regulated. The isoleucin conjugate of jasmonic acid (JA-IIe) is a major regulatory molecule. We have previously shown that inositol polyphosphate signals are required for defense responses in Arabidopsis; however, the way in which inositol polyphosphates contribute to plant responses to wounding has so far remained unclear. Arabidopsis F-box proteins involved in the perception of JA-IIe (COI1) and auxin (TIR1) are structurally similar. Because TIR1 has recently been shown to contain inositol hexakisphosphate (InsP(6)) as a co-factor of unknown function, here we explored the possibility that InsP(6) or another inositol polyphosphate is required for COI1 function. In support of this hypothesis, COI1 variants with changes in putative inositol polyphosphate coordinating residues exhibited a reduced interaction with the COI1 target, JAZ9, in yeast two-hybrid tests. The equivalent COI1 variants displayed a reduced capability to rescue jasmonate-mediated root growth inhibition or silique development in Arabidopsis col1 mutants. Yeast two-hybrid tests using wild-type COI1 in an ipk1 Delta yeast strain exhibiting increased levels of inositol pentakisphosphate (InsP(5)) and reduced levels of InsP(6) indicate an enhanced COI1/JAZ9 interaction. Consistent with these findings, Arabidopsis ipk1-1 mutants, also with increased InsP(5) and reduced InsP(6) levels, showed increased defensive capabilities via COI1-mediated processes, including wound-induced gene expression, defense against caterpillars or root growth inhibition by jasmonate. The combined data from experiments using mutated COI1 variants, as well as yeast and Arabidopsis backgrounds altered in inositol polyphosphate metabolism, indicate that an inositol polyphosphate, and probably InsP(5), contributes to COI1 function."],["dc.identifier.doi","10.1111/j.1365-313X.2011.04480.x"],["dc.identifier.isi","000288449700009"],["dc.identifier.pmid","21205029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23810"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0960-7412"],["dc.title","Jasmonic acid perception by COI1 involves inositol polyphosphates in Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","277"],["dc.bibliographiccitation.lastpage","291"],["dc.bibliographiccitation.seriesnr","16"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Heilmann, Ingo"],["dc.contributor.editor","Munnik, Teun"],["dc.date.accessioned","2018-09-13T06:48:33Z"],["dc.date.available","2018-09-13T06:48:33Z"],["dc.date.issued","2010"],["dc.description.abstract","Oxylipins are derived from the oxidation of polyunsaturated fatty acids. Further conversion of the resulting fatty acid hydroperoxides gives rise to a multitude of oxylipin classes, including hydroxy-, oxo-, or keto fatty acids, volatile aldehydes, and the phytohormone, jasmonic acid (JA). Oxylipins may be structurally further diversified by esterification, i.e., to plastidial glycolipids, Arabidopsides, or conjugation to amino acids. Oxylipin research so far has focused mainly on the investigation of jasmonates and their roles in wound signaling and plant development. In contrast, the physiological roles of other oxylipins are by far less well understood, in part because enzymes responsible for their formation are not well characterized. This chapter aims at giving an overview of plant oxylipin signaling, highlighting recent discoveries of new roles for different oxylipins in the regulation of developmental or adaptational processes."],["dc.identifier.doi","10.1007/978-3-642-03873-0_18"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15699"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.publisher","Springer"],["dc.publisher.place","Berlin, Heidelberg"],["dc.relation.crisseries","Plant Cell Monographs"],["dc.relation.doi","10.1007/978-3-642-03873-0"],["dc.relation.isbn","978-3-642-03872-3"],["dc.relation.isbn","978-3-642-03873-0"],["dc.relation.ispartof","Lipid Signaling in Plants"],["dc.relation.ispartofseries","Plant Cell Monographs; 16"],["dc.title","Oxylipin Signaling and Plant Growth"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","197"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Analytical Biochemistry"],["dc.bibliographiccitation.lastpage","201"],["dc.bibliographiccitation.volume","378"],["dc.contributor.author","Koenig, Sabine"],["dc.contributor.author","Hoffmann, Mareike"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T11:12:57Z"],["dc.date.available","2018-11-07T11:12:57Z"],["dc.date.issued","2008"],["dc.description.abstract","Recent advances in research on the physiological roles of phosphoinositides in eukaryotic organisms indicate a need to distinguish molecular phosphoinositide species on the basis of their characteristic head groups as well as their glycerolipid moieties. Accurate identification of phosphoinositide species in biological samples poses an analytical challenge, because structurally similar inositol phosphate head groups must be resolved, as must lipid-associated fatty acids. Although intact phosphoinositide species have been successfully analyzed, such analyses employ state-of-the-art liquid chromatography/mass spectrometry and require expensive equipment not accessible to many researchers. Described here is a cost-efficient and reliable alternative developed by adaptation of a combination of classic methods for lipid analysis, thin-layer chromatography and gas chromatography. (C) 2008 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.ab.2008.03.052"],["dc.identifier.isi","000256846800014"],["dc.identifier.pmid","18466755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53781"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0003-2697"],["dc.title","Determination of content and fatty acid composition of unlabeled phosphoinositide species by thin-layer chromatography and gas chromatography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]