Heilmann, Ingo

[["dc.bibliographiccitation.firstpage","452"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Plants"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Vu-Becker, Linh Hai"],["dc.contributor.author","Riechmann, Mara"],["dc.contributor.author","Krishnamoorthy, Praveen"],["dc.contributor.author","Fratini, Marta"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2021-04-14T08:26:23Z"],["dc.date.available","2021-04-14T08:26:23Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/plants9040452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81922"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","2223-7747"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Coordinated Localization and Antagonistic Function of NtPLC3 and PI4P 5-Kinases in the Subapical Plasma Membrane of Tobacco Pollen Tubes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.issue","1-4"],["dc.bibliographiccitation.journal","PROTOPLASMA"],["dc.bibliographiccitation.lastpage","31"],["dc.bibliographiccitation.volume","240"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Seiler, Stephan"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T08:44:51Z"],["dc.date.available","2018-11-07T08:44:51Z"],["dc.date.issued","2010"],["dc.description.abstract","Phosphoinositides (PIs) are minor, but essential phospholipid constituents of eukaryotic membranes, and are involved in the regulation of various physiological processes. Recent genetic and cell biological advances indicate that PIs play important roles in the control of polar tip growth in plant cells. In root hairs and pollen tubes, PIs control directional membrane trafficking required for the delivery of cell wall material and membrane area to the growing tip. So far, the exact mechanisms by which PIs control polarity and tip growth are unresolved. However, data gained from the analysis of plant, fungal and animal systems implicate PIs in the control of cytoskeletal dynamics, ion channel activity as well as vesicle trafficking. The present review aims at giving an overview of PI roles in eukaryotic cells with a special focus on functions pertaining to the control of cell polarity. Comparative screening of plant and fungal genomes suggests diversification of the PI system with increasing organismic complexity. The evolutionary conservation of the PI system among eukaryotic cells suggests a role for PIs in tip growing cells in models where PIs so far have not been a focus of attention, such as fungal hyphae."],["dc.identifier.doi","10.1007/s00709-009-0093-0"],["dc.identifier.isi","000275750200003"],["dc.identifier.pmid","20091065"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4183"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20290"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.relation.issn","0033-183X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","At the poles across kingdoms: phosphoinositides and polar tip growth"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","387"],["dc.bibliographiccitation.journal","Biochemical Journal"],["dc.bibliographiccitation.lastpage","399"],["dc.bibliographiccitation.volume","415"],["dc.contributor.author","Koenig, Sabine"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Lerche, Jennifer"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T11:09:18Z"],["dc.date.available","2018-11-07T11:09:18Z"],["dc.date.issued","2008"],["dc.description.abstract","Plants exposed to hyperosmotic stress undergo changes in membrane dynamics and lipid composition to maintain cellular integrity and avoid membrane leakage. Various plant species respond to hyperosmotic stress with transient increases in PtdIns(4,5)P-2; however, the physiological role of such increases is unresolved. The plasma membrane represents the outermost barrier between the symplast of plant cells and its apoplastic surroundings. In the present Study, the spatio-temporal dynamics of stress-induced changes in phosphoinositides were analysed in subcellular fractions of Arabidopsis leaves to delineate possible physiological roles. Unlabelled lipids were separated by TLC and quantified by gas-chromatographic detection of associated fatty acids. Transient PtdIns(4,5)P-2 increases upon exposure to hyperosmotic stress were detected first in enriched plasmamembrane fractions, however, at later time points, PtdIns(4,5)P-2 was increased in the endomembrane fractions of the corresponding two-phase systems. When major endomembranes were enriched from rosette leaves prior to hyperosmotic stress and during stimulation for 60 min, no stress-induced increases in the levels of PtdIns(4,5)P-2 were found in fractions enriched for endoplasmic reticulum, nuclei or plastidial membranes. Instead, increased PtdIns(4,5)P-2 was found in CCVs (clathrin-coated vesicles), which proliferated several-fold in mass within 60 min of hyperosmotic stress, according to the abundance of CCV-associated proteins and lipids. Monitoring the subcellular distribution of fluorescence-tagged reporters for clathrin and PtdIns(4,5)P-2 during transient co-expression in onion epidermal cells indicates rapid stress-induced co-localization of clathrin with PtdIns(4,5)P-2 at the plasma membrane. The results indicate that PtdIns(4,5)P-2 may act in stress-induced formation of CCVs in plant cells, highlighting the evolutionary conservation of the phosphoinositide system between organismic kingdoms."],["dc.description.sponsorship","German Research Foundation [DFG (Deutsche Forschungsgemeinschaft)]"],["dc.identifier.doi","10.1042/BJ20081306"],["dc.identifier.isi","000260649700006"],["dc.identifier.pmid","18721128"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52975"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Portland Press Ltd"],["dc.relation.issn","1470-8728"],["dc.relation.issn","0264-6021"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Salt-stress-induced association of phosphatidylinositol 4,5-bisphosphate with clathrin-coated vesicles in plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e51454"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Maehs, Anette"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Heilig, Yvonne"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Hempel, Franziska"],["dc.contributor.author","Seiler, Stephan"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T09:02:18Z"],["dc.date.available","2018-11-07T09:02:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Fungal hyphae and plant pollen tubes are among the most highly polarized cells known and pose extraordinary requirements on their cell polarity machinery. Cellular morphogenesis is driven through the phospholipid-dependent organization at the apical plasma membrane. We characterized the contribution of phosphoinositides (PIs) in hyphal growth of the filamentous ascomycete Neurospora crassa. MSS-4 is an essential gene and its deletion resulted in spherically growing cells that ultimately lyse. Two conditional mss-4-mutants exhibited altered hyphal morphology and aberrant branching at restrictive conditions that were complemented by expression of wild type MSS-4. Recombinant MSS-4 was characterized as a phosphatidylinositolmonophosphate-kinase phosphorylating phosphatidylinositol 4-phosphate (PtdIns4P) to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2). PtdIns3P was also used as a substrate. Sequencing of two conditional mss-4 alleles identified a single substitution of a highly conserved Y750 to N. The biochemical characterization of recombinant protein variants revealed Y750 as critical for PI4P 5-kinase activity of MSS-4 and of plant PI4P 5-kinases. The conditional growth defects of mss-4 mutants were caused by severely reduced activity of MSS-4(Y750N), enabling the formation of only trace amounts of PtdIns(4,5)P-2. In N. crassa hyphae, PtdIns(4,5)P-2 localized predominantly in the plasma membrane of hyphae and along septa. Fluorescence-tagged MSS-4 formed a subapical collar at hyphal tips, localized to constricting septa and accumulated at contact points of fusing N. crassa germlings, indicating MSS-4 is responsible for the formation of relevant pools of PtdIns(4,5)P-2 that control polar and directional growth and septation. N. crassa MSS-4 differs from yeast, plant and mammalian PI4P 5-kinases by containing additional protein domains. The N-terminal domain of N. crassa MSS-4 was required for correct membrane association. The data presented for N. crassa MSS-4 and its roles in hyphal growth are discussed with a comparative perspective on PI-control of polar tip growth in different organismic kingdoms."],["dc.identifier.doi","10.1371/journal.pone.0051454"],["dc.identifier.isi","000312386600026"],["dc.identifier.pmid","23272106"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8449"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24652"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","The Essential Phosphoinositide Kinase MSS-4 Is Required for Polar Hyphal Morphogenesis, Localizing to Sites of Growth and Cell Fusion in Neurospora crassa"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Erwig, Michelle S."],["dc.contributor.author","Patzig, Julia"],["dc.contributor.author","Steyer, Anna M."],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Heilmann, Mareike"],["dc.contributor.author","Heilmann, Ingo"],["dc.contributor.author","Jung, Ramona B."],["dc.contributor.author","Kusch, Kathrin"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Jahn, Olaf"],["dc.contributor.author","Nave, Klaus-Armin"],["dc.contributor.author","Werner, Hauke B."],["dc.date.accessioned","2019-08-02T06:29:41Z"],["dc.date.available","2019-08-02T06:29:41Z"],["dc.date.issued","2019"],["dc.description.abstract","Myelin serves as an axonal insulator that facilitates rapid nerve conduction along axons. By transmission electron microscopy, a healthy myelin sheath comprises compacted membrane layers spiraling around the cross-sectioned axon. Previously we identified the assembly of septin filaments in the innermost non-compacted myelin layer as one of the latest steps of myelin maturation in the central nervous system (CNS) (Patzig et al., 2016). Here we show that loss of the cytoskeletal adaptor protein anillin (ANLN) from oligodendrocytes disrupts myelin septin assembly, thereby causing the emergence of pathological myelin outfoldings. Since myelin outfoldings are a poorly understood hallmark of myelin disease and brain aging we assessed axon/myelin-units in Anln-mutant mice by focused ion beam-scanning electron microscopy (FIB-SEM); myelin outfoldings were three-dimensionally reconstructed as large sheets of multiple compact membrane layers. We suggest that anillin-dependent assembly of septin filaments scaffolds mature myelin sheaths, facilitating rapid nerve conduction in the healthy CNS."],["dc.identifier.doi","10.7554/eLife.43888"],["dc.identifier.pmid","30672734"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15932"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62262"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","2050-084X"],["dc.relation.issn","2050-084X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","764"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Metabolomics"],["dc.bibliographiccitation.lastpage","777"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Heilmann, Ingo"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Meinicke, Peter"],["dc.date.accessioned","2018-11-07T09:56:52Z"],["dc.date.available","2018-11-07T09:56:52Z"],["dc.date.issued","2015"],["dc.description.abstract","A central aim in the evaluation of non-targeted metabolomics data is the detection of intensity patterns that differ between experimental conditions as well as the identification of the underlying metabolites and their association with metabolic pathways. In this context, the identification of metabolites based on non-targeted mass spectrometry data is a major bottleneck. In many applications, this identification needs to be guided by expert knowledge and interactive tools for exploratory data analysis can significantly support this process. Additionally, the integration of data from other omics platforms, such as DNA microarray-based transcriptomics, can provide valuable hints and thereby facilitate the identification of metabolites via the reconstruction of related metabolic pathways. We here introduce the MarVis-Pathway tool, which allows the user to identify metabolites by annotation of pathways from cross-omics data. The analysis is supported by an extensive framework for pathway enrichment and meta-analysis. The tool allows the mapping of data set features by ID, name, and accurate mass, and can incorporate information from adduct and isotope correction of mass spectrometry data. MarVis-Pathway was integrated in the MarVis-Suite (http://marvis.gobics.de), which features the seamless highly interactive filtering, combination, clustering, and visualization of omics data sets. The functionality of the new software tool is illustrated using combined mass spectrometry and DNA microarray data. This application confirms jasmonate biosynthesis as important metabolic pathway that is upregulated during the wound response of Arabidopsis plants."],["dc.identifier.doi","10.1007/s11306-014-0734-y"],["dc.identifier.isi","000354137100020"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37052"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1573-3890"],["dc.relation.issn","1573-3882"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","MarVis-Pathway: integrative and exploratory pathway analysis of non-targeted metabolomics data"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","114"],["dc.bibliographiccitation.journal","Frontiers in Plant Science"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Stenzel, Irene"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Quint, Marcel"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T09:16:17Z"],["dc.date.available","2018-11-07T09:16:17Z"],["dc.date.issued","2012"],["dc.description.abstract","The apical plasma membrane of pollen tubes contains different PI4P 5-kinases that all produce phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P-2] but exert distinct cellular effects. In the present example, overexpression of Arabidopsis AtPIP5K5 or tobacco NtPIP5K6-1 caused growth defects previously attributed to increased pectin secretion. In contrast, overexpression of Arabidopsis AtPIP5K2 caused apical tip swelling implicated in altering actin fine structure in the pollen tube apex. AtPIP5K5, NtPIP5K6-1, and AtPIP5K2 share identical domain structures. Domains required for correct membrane association of the enzymes were identified by systematic deletion of N-terminal domains and subsequent expression of fluorescence-tagged enzyme truncations in tobacco pollen tubes. A variable linker region (Lin) contained in all PI4P 5-kinase isoforms of subfamily B, but not conserved in sequence, was recognized to be necessary for correct subcellular localization of AtPIP5K5, NtPIP5K6-1, and AtPIP5K2. Deletion of N-terminal domains including the Lin domain did not impair catalytic activity of recombinant AtPIP5K5, NtPIP5K6-1, or AtPIP5K2 in vitro; however, the presence of the Lin domain was necessary for in vivo effects on pollen tube growth upon overexpression of truncated enzymes. Overexpression of catalytically inactive variants of AtPIP5K5, NtPIP5K6-1, or AtPIP5K2 did not influence pollen tube growth, indicating that PtdIns(4,5)P2 production rather than structural properties of PI4P 5-kinases was relevant for the manifestation of growth phenotypes. When Lin domains were swapped between NtPIP5K6-1 and AtPIP5K2 and the chimeric enzymes overexpressed in pollen tubes, the chimeras reciprocally gained the capabilities to invoke tip swelling or secretion phenotypes, respectively. The data indicate that the Lin domain directed the enzymes into different regulatory contexts, possibly contributing to channeling of PtdIns(4,5)P-2 at the interface of secretion and actin cytoskeleton."],["dc.identifier.doi","10.3389/fpls.2011.00114"],["dc.identifier.isi","000208837800010"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8728"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27901"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Research Foundation"],["dc.relation.eissn","1664-462X"],["dc.relation.issn","1664-462X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Variable regions of PI4P 5-kinases direct PtdIns(4,5)P-2 toward alternative regulatory functions in tobacco pollen tubes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]