Czerny, Claus-Peter

[["dc.bibliographiccitation.journal","Frontiers in Veterinary Science"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Roller, Marco"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Knauf-Witzens, Tobias"],["dc.contributor.author","Oelemann, Walter M. R."],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Goethe, Ralph"],["dc.date.accessioned","2021-04-14T08:30:11Z"],["dc.date.available","2021-04-14T08:30:11Z"],["dc.date.issued","2020"],["dc.description.abstract","Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis (ParaTB or Johne's disease), a contagious, chronic and typically fatal enteric disease of domestic and non-domestic ruminants. Clinically affected animals present wasting and emaciation. However, MAP can also infect non-ruminant animal species with less specific signs. Zoological gardens harbor various populations of diverse animal species, which are managed on limited space at higher than natural densities. Hence, they are predisposed to endemic trans-species pathogen distribution. Information about the incidence and prevalence of MAP infections in zoological gardens and the resulting potential threat to exotic and endangered species are rare. Due to unclear pathogenesis, chronicity of disease as well as the unknown cross-species accuracy of diagnostic tests, diagnosis and surveillance of MAP and ParaTB is challenging. Differentiation between uninfected shedders of ingested bacteria; subclinically infected individuals; and preclinically diseased animals, which may subsequently develop clinical signs after long incubation periods, is crucial for the interpretation of positive test results in animals and the resulting consequences in their management. This review summarizes published data from the current literature on occurrence of MAP infection and disease in susceptible and affected zoo animal species as well as the applied diagnostic methods and measures. Clinical signs indicative for ParaTB, pathological findings and reports on detection, transmission and epidemiology in zoo animals are included. Furthermore, case reports were re-evaluated for incorporation into accepted consistent terminologies and case definitions."],["dc.identifier.doi","10.3389/fvets.2020.572724"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83135"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","2297-1769"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Mycobacterium avium Subspecies paratuberculosis Infection in Zoo Animals: A Review of Susceptibility and Disease Process"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1007"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Okuni, Julius Boniface"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Eltom, Kamal H."],["dc.contributor.author","Eltayeb, ElSagad"],["dc.contributor.author","Amanzada, Ahmad"],["dc.contributor.author","Omega, Joseph Amesa"],["dc.contributor.author","Czerny, Claus Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Ojok, Lonzy"],["dc.date.accessioned","2021-04-14T08:25:03Z"],["dc.date.available","2021-04-14T08:25:03Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/microorganisms8071007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81506"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2076-2607"],["dc.title","Paratuberculosis: A Potential Zoonosis and a Neglected Disease in Africa"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.journal","Journal of Clinical Virology"],["dc.bibliographiccitation.lastpage","27"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Bekaert, Michaël"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T14:25:03Z"],["dc.date.available","2020-12-10T14:25:03Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.jcv.2018.07.001"],["dc.identifier.issn","1386-6532"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72419"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Combination random isothermal amplification and nanopore sequencing for rapid identification of the causative agent of an outbreak"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","117"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Roller, Marco"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Knauf-Witzens, Tobias"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Goethe, Ralph"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2021-04-14T08:32:27Z"],["dc.date.available","2021-04-14T08:32:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/vetsci7030117"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17560"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83927"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2306-7381"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Molecular and Serological Footprints of Mycobacterium avium Subspecies Infections in Zoo Animals"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","50"],["dc.bibliographiccitation.journal","Journal of Virological Methods"],["dc.bibliographiccitation.lastpage","53"],["dc.bibliographiccitation.volume","263"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Dill, Veronika"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Eschbaumer, Michael"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Hoffmann, Bernd"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T15:20:09Z"],["dc.date.available","2020-12-10T15:20:09Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.jviromet.2018.10.020"],["dc.identifier.issn","0166-0934"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72571"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Serotyping of foot-and-mouth disease virus using oxford nanopore sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","123"],["dc.bibliographiccitation.lastpage","136"],["dc.bibliographiccitation.seriesnr","2142"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Bekaert, Michaël"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.editor","Kobinger, Gary"],["dc.contributor.editor","Racine, Trina"],["dc.date.accessioned","2021-06-02T10:44:21Z"],["dc.date.available","2021-06-02T10:44:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-1-0716-0581-3_11"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87005"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer US"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-0716-0581-3"],["dc.relation.isbn","978-1-0716-0580-6"],["dc.relation.ispartof","Methods in Molecular Biology"],["dc.relation.ispartof","Zika Virus : Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 2142"],["dc.title","Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","3648"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Hotop, Sven-Kevin"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Ndiaye, Oumar"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Pessôa, Rodrigo"],["dc.contributor.author","Hufert, Frank"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Frank, Ronald"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Schmidt-Chanasit, Jonas"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Niedrig, Matthias"],["dc.contributor.author","Brönstrup, Mark"],["dc.contributor.author","Fritz, Hans-Joachim"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:50:13Z"],["dc.date.available","2019-07-09T11:50:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Zika virus (ZIKV) is a mosquito-borne flavivirus. Homologous proteins of different flaviviruses display high degrees of sequence identity, especially within subgroups. This leads to extensive immunological cross-reactivity and corresponding problems for developing a ZIKV-specific serological assay. In this study, peptide microarrays were employed to identify individual ZIKV antibody targets with promise in differential diagnosis. A total of 1643 overlapping oligopeptides were synthesized and printed onto glass slides. Together, they encompass the full amino acid sequences of ZIKV proteomes of African, Brazilian, USA, and French Polynesian origins. The resulting ZIKV scanning microarray chips were used to screen three pools of sera from recent Zika outbreaks in Senegal and Cape Verde, in Brazil, and from overseas travelers returning to the EU. Together with a mixed pool of well characterized, archived sera of patients suffering from infections by dengue, yellow fever, tick-borne encephalitis, and West Nile viruses, a total of 42 sera went into the study. Sixty-eight antibody target regions were identified. Most of which were hitherto unknown. Alignments and sequence comparisons revealed 13 of which could be classified as bona fide ZIKV-specific. These identified antibody target regions constitute a founding set of analytical tools for serological discrimination of ZIKV from other flaviviruses."],["dc.identifier.doi","10.1038/s41598-019-40224-2"],["dc.identifier.pmid","30842564"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59723"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","630"],["dc.title","Diagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0168733"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Schäfer, Jenny"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:43:02Z"],["dc.date.available","2019-07-09T11:43:02Z"],["dc.date.issued","2016"],["dc.description.abstract","BACKGROUND: The detection of Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants is crucial to control spread among animals and to humans. Cultivation of MAP is seen as the gold standard for detection, although it is very time consuming and labour intensive. In addition, several PCR assays have been developed to detect MAP in around 90 minutes, but these assays required highly sophisticated equipment as well as lengthy and complicated procedure. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we have developed a rapid assay for the detection of MAP based on the recombinase polymerase amplification (RPA) assay targeting a MAP specific region, the IS900 gene. The detection limit was 16 DNA molecules in 15 minutes as determined by the probit analysis on eight runs of the plasmid standard. Cross reactivity with other mycobacterial and environmentally associated bacterial strains was not observed. The clinical performance of the MAP RPA assay was tested using 48 MAP-positive and 20 MAP-negative blood, sperm, faecal and tissue samples. All results were compared with reads of a highly sensitive real-time PCR assay. The specificity of the MAP RPA assay was 100%, while the sensitivity was 89.5%. CONCLUSIONS/SIGNIFICANCE: The RPA assay is quicker and much easier to handle than real-time PCR. All RPA reagents were cold-chain independent. Moreover, combining RPA assay with a simple extraction protocol will maximize its use at point of need for rapid detection of MAP."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1371/journal.pone.0168733"],["dc.identifier.pmid","27992571"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58806"],["dc.language.iso","en"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","Development of a Recombinase Polymerase Amplification Assay for Rapid Detection of the Mycobacterium avium subsp. paratuberculosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","29"],["dc.bibliographiccitation.journal","Analytical Biochemistry"],["dc.bibliographiccitation.lastpage","33"],["dc.bibliographiccitation.volume","544"],["dc.contributor.author","Kissenkötter, Jonas"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Ademowo, Olusegun George"],["dc.contributor.author","Oyinloye, Oladapo Elijah"],["dc.contributor.author","Bakarey, Adeleye Solomon"],["dc.contributor.author","Dobler, Gerhard"],["dc.contributor.author","Tappe, Dennis"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T14:14:51Z"],["dc.date.available","2020-12-10T14:14:51Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.ab.2017.12.018"],["dc.identifier.issn","0003-2697"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71522"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Development of a pan-rickettsial molecular diagnostic test based on recombinase polymerase amplification assay"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","36"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Diagnostics (Basel, Switzerland)"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Roller, Marco"],["dc.contributor.author","Alslim, Lamia M. A."],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-29T15:02:58Z"],["dc.date.available","2019-07-29T15:02:58Z"],["dc.date.issued","2019"],["dc.description.abstract","The rapid identification of Mycobacteriumavium subspecies paratuberculosis (MAP) infected animals within the herd is essential for preventing the spread of the disease as well as avoiding human exposure. Although culture is seen as the gold standard, there are various molecular assays available i.e., polymerase chain reaction (PCR) or isothermal amplification technique (recombinase polymerase amplification (RPA)) for the detection of MAP. The accuracy of the molecular assays is highly dependent on the DNA extraction method. In order to establish a rapid point of need system for the detection of MAP DNA from stool samples, we developed a rapid DNA extraction protocol (MAP DNA SpeedXtract) specified for use in combination with the RPA. The whole procedure from \"sample in\" to \"result out\" was conducted in a mobile suitcase laboratory. The DNA extraction is based on reverse purification by magnetic beads, which reduces the required technical demand. The MAP DNA SpeedXtract was performed within 25 min and only three pipetting steps were needed. The amplification and detection time were 20 min in RPA. The sensitivity and specificity of the developed protocol in comparison with the lab-based silica membrane column extraction and real-time PCR were 90.9% (n = 22) and 100% (n = 23), respectively. In conclusion, we established a rapid and reliable protocol for the extraction and detection of MAP DNA. All reagents are cold chain independent. The entire setup is ideal for point of need identification of MAP infected cases."],["dc.identifier.doi","10.3390/diagnostics9020036"],["dc.identifier.pmid","30934956"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16310"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62152"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2075-4418"],["dc.relation.issn","2075-4418"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Development of Rapid Extraction Method of Mycobacterium avium Subspecies paratuberculosis DNA from Bovine Stool Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]