Czerny, Claus-Peter

[["dc.bibliographiccitation.firstpage","303"],["dc.bibliographiccitation.issue","7-8"],["dc.bibliographiccitation.journal","BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT"],["dc.bibliographiccitation.lastpage","312"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.date.accessioned","2018-11-07T08:54:39Z"],["dc.date.available","2018-11-07T08:54:39Z"],["dc.date.issued","2011"],["dc.description.abstract","During the last years, bacteriophages have been used as valuable tool in microbiological diagnostics and basic research. Even though the potential of bacteriophages for fighting bacterial pathogens is known for a long time, the phage therapy is not used in daily routine worldwide. Due to the continuing spread of bacterial resistance to antimicrobials and an increasing awareness of the necessity to protect consumers' health, the phage therapy meanwhile has become a subject of major interest in veterinary medicine, too. This article is meant to present fields of application for phage therapy as well as current trends of development in the field of livestock animals."],["dc.identifier.doi","10.2376/0005-9366-124-303"],["dc.identifier.isi","000292802800006"],["dc.identifier.pmid","21848038"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22718"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Schluetersche Verlagsgesellschaft Mbh & Co Kg"],["dc.relation.issn","0005-9366"],["dc.title","Phage therapy in the fields of veterinary medicine: an overview"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","297"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Urstadt, S."],["dc.contributor.author","Karapetyan, A."],["dc.contributor.author","Thebille, S."],["dc.contributor.author","Niederhausen, M."],["dc.contributor.author","Schmelz, F."],["dc.contributor.author","Czerny, C.-P."],["dc.date.accessioned","2018-11-07T10:59:03Z"],["dc.date.available","2018-11-07T10:59:03Z"],["dc.date.issued","2007"],["dc.format.extent","14"],["dc.identifier.isi","000250104800029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50605"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","59th Annual Meeting of the Deutschen-Gesellschaft-fur-Hygiene-und-Mikrobiologie"],["dc.relation.eventlocation","Gottingen, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Development of semi-nested and quantitative real time pool PCR methods for the detection of Mycobacterium avium subsp paratuberculosis in seropositive and seronegative dairy cows"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","197"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Veterinary Microbiology"],["dc.bibliographiccitation.lastpage","201"],["dc.bibliographiccitation.volume","154"],["dc.contributor.author","Muenster, Pia"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Wemheuer, Wilhelm E."],["dc.contributor.author","Petschenka, Jutta"],["dc.contributor.author","Wemheuer, Wiebke M."],["dc.contributor.author","Steinbrunn, Christina"],["dc.contributor.author","Campe, Amely"],["dc.contributor.author","Schulz-Schaeffer, Walter J."],["dc.contributor.author","Kreienbrock, Lothar"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.date.accessioned","2018-11-07T08:48:45Z"],["dc.date.available","2018-11-07T08:48:45Z"],["dc.date.issued","2011"],["dc.description.abstract","The aim of this study was to investigate the occurrence of subclinical Mycobacterium avium spp. paratuberculosis (MAP) infections at slaughter by testing ileocaecal lymph nodes with a semi-nested IS900 PCR. Tissue samples were available within the framework of a parallel study investigating BSE-susceptibility factors in members of BSE-cohorts in the German Federal State of Lower Saxony. Ileocaecal lymph nodes were collected over a 2-year sampling period from 99 slaughter cattle of a mean age of 6.5 years (5.5-7.5 years). A recently developed IS900 semi-nested polymerase chain reaction (snPCR) assay offering a sensitivity of 1 genome equivalent was used for the detection of MAP-DNA. Based on this snPCR, 17 out of the 99 samples gave positive results, indicating a MAP occurrence of 17.17% in the random sample. All PCR products were sequenced for screening of polymorphisms. Nucleotide homologies of 98.5-100% were found with respect to the MAP K10 reference sequence IS900 (GenBank: AEI 6958). PCR analysis of ileocaecal lymph nodes collected from slaughter cattle proved to be a suitable technique to determine MAP occurrence in the local cattle population. (C) 2011 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.vetmic.2011.06.033"],["dc.identifier.isi","000297778200025"],["dc.identifier.pmid","21775077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21299"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0378-1135"],["dc.title","Detection of Mycobacterium avium ssp paratuberculosis in ileocaecal lymph nodes collected from elderly slaughter cows using a semi-nested IS900 polymerase chain reaction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","176"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Zoo and Wildlife Medicine"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Muenster, Pia"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","von Buchholz, Aloys"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.date.accessioned","2018-11-07T09:27:57Z"],["dc.date.available","2018-11-07T09:27:57Z"],["dc.date.issued","2013"],["dc.description.abstract","An alpaca (Vicugna pacos) bred and kept in a German zoological garden exhibited clinical signs consistent with paratuberculosis. The presence of Mycobacterium avium ssp. paratuberculosis (MAP) was confirmed in feces and in the ileocecal lymph node (ILN) by IS900-based polymerase chain reaction (PCR) assays and culture. A bacterial burden of 7.63106 MAP/g in feces and 4.43107 MAP/g in lymph node tissue was determined by real-time PCR. For further characterization, a conventional PCR was developed. After sequencing of the 864bp PCR amplicon covering nucleotide positions 13 to 876 within the IS900, the alpaca isolate shared 100% nucleotide homology with the bovine MAP-K10 IS900 reference sequence (GenBank: AE16958), indicating a cattle strain. This report supports the present occurrence of MAP in German camelid populations and highlights the need to expand routine MAP surveillance to South American camelids held in European zoos."],["dc.description.sponsorship","Federal Ministry of Economics and Technology [KF2484201AJ9]"],["dc.identifier.isi","000339619500028"],["dc.identifier.pmid","23505723"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30661"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Zoo Veterinarians"],["dc.relation.issn","1937-2825"],["dc.relation.issn","1042-7260"],["dc.title","DETECTION OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS BY IS900-BASED PCR ASSAYS FROM AN ALPACA (VICUGNA PACOS) KEPT IN A GERMAN ZOOLOGICAL GARDEN"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","116"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Veterinary Microbiology"],["dc.bibliographiccitation.lastpage","123"],["dc.bibliographiccitation.volume","163"],["dc.contributor.author","Muenster, Pia"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","von Buchholz, Aloys"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.date.accessioned","2018-11-07T09:26:02Z"],["dc.date.available","2018-11-07T09:26:02Z"],["dc.date.issued","2013"],["dc.description.abstract","Little data concerning the distribution of Mycobacterium avium ssp. paratuberculosis (MAP) in zoological gardens is available. The presence of MAP in captured wildlife might provide further information on non-ruminant hosts and expand the list of animals susceptible to MAP being potential sources of MAP transmission. Therefore, a German zoological garden with recent history of clinical paratuberculosis in Barbary sheep (Ammo tragus lervia) and an alpaca (Lama pacos) was selected to estimate the distribution of MAP infections in 21 mammalian and avian species. Pooled faecal samples from individual animals of each species were tested for the presence of MAP. A previously developed 15900 semi-nested PCR (snPCR) assay, amplifying a 587 bp and a 278 bp fragment, was used for the detection of MAP-DNA. Based on this snPCR, in 14 out of the 21 pooled faecal samples MAP-DNA was detected. MAP positive snPCR results were observed in ruminants and camelids as well as in non-ruminants such as equines, primates, rodents, and birds. Moreover, a quantitative real-time PCR demonstrated that the concentration of MAP-DNA was within the range of 2.2 x 10(3)-9.6 x 10(6) MAP-DNA equivalents per gram faeces. The highest amount was shed by primates such as Black-and-white ruffed lemurs (Varecia variegata) and Cottontop tamarins (Saguinus oedipus). This is the first survey investigating the presence of MAP in a German zoo, which includes non-ruminants. The results of the present study confirm the wide host range of MAP and demonstrate that MAP occurs more frequently in zoo animals than expected. In order to restrict further spread of MAP in European zoos, additional investigations regarding the existing transmission pathways of MAP in zoos are recommended. (C) 2013 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","Federal Ministry of Economics and Technology [KF2484201AJ9]"],["dc.identifier.doi","10.1016/j.vetmic.2012.12.009"],["dc.identifier.isi","000316647000014"],["dc.identifier.pmid","23298700"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30204"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0378-1135"],["dc.title","Distribution of Mycobacterium avium ssp paratuberculosis in a German zoological garden determined by IS900 semi-nested and quantitative real-time PCR"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","175"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Transboundary and Emerging Diseases"],["dc.bibliographiccitation.lastpage","187"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Muenster, Pia"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Wemheuer, W."],["dc.contributor.author","Schwarz, Daniel"],["dc.contributor.author","Doering, Stefan"],["dc.contributor.author","Czerny, C-P"],["dc.date.accessioned","2018-11-07T09:26:54Z"],["dc.date.available","2018-11-07T09:26:54Z"],["dc.date.issued","2013"],["dc.description.abstract","Johne's disease is caused by Mycobacterium avium ssp. paratuberculosis (MAP) and has been recognized as an important bacterial infection in ruminants. Although MAP has been detected in semen and within the reproductive organs of bulls, the bacterial distribution and shedding patterns are currently not well characterized. Our investigation was performed to detect and quantify MAP in faeces, semen and blood samples repeatedly drawn from a naturally infected but asymptomatic 18-month-old German Simmental breeding bull candidate over a period of 3years (June 2007November 2010). Qualitative and quantitative polymerase chain reaction (PCR) techniques were used to correlate the presence and matrix-specific amounts of MAP. In total, 65 sampling dates were selected. Mycobacterium avium ssp. paratuberculosis was detected intermittently in all matrices with MAP-free intervals of up to 18weeks by an IS900 semi-nested PCR. The number of MAP-positive results from semen and blood samples was higher than from faecal samples. A quantitative polymerase chain reaction detected the highest MAP contents in faeces (103106MAP/g), while lower amounts were found in semen and blood samples (102105MAP/ml). Although no significant agreement was calculated between the presence of MAP in faeces and blood, a statistically significant positive correlation between its occurrence in semen and blood was determined (r=0.38, P<0.05, n=29). The present study contributes to a more detailed understanding of MAP distribution patterns in faeces, semen and blood of a subclinically infected breeding bull candidate. It highlights the possible role of breeding bulls as a source of MAP transmission and indicates the need for further monitoring and hygienic measures to prevent the spread of the infection via semen."],["dc.description.sponsorship","Federal Ministry of Economics and Technology [KF2484201AJ9]"],["dc.identifier.doi","10.1111/j.1865-1682.2012.01336.x"],["dc.identifier.isi","000316078200011"],["dc.identifier.pmid","22571476"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30406"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1865-1674"],["dc.title","A Longitudinal Study to Characterize the Distribution Patterns of Mycobacterium avium ssp paratuberculosis in Semen, Blood and Faeces of a Naturally Infected Bull by IS 900 Semi-Nested and Quantitative Real-Time PCR"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","71"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT"],["dc.bibliographiccitation.lastpage","77"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Schmitz, Christina"],["dc.contributor.author","Moors, Eva"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.date.accessioned","2018-11-07T09:00:49Z"],["dc.date.available","2018-11-07T09:00:49Z"],["dc.date.issued","2011"],["dc.description.abstract","During the fattening period of a broiler flock four different litter materials (peat, chopped straw, shavings, silage) were tested simultaneously. The separated sections were tested for the presence of Salmonella bacteria using the sock-sampling method as described in the regulation EC No. 646/2007 with slight modifications in the sampling technique and the laboratory protocol. In addition, some chemical and physical parameters regarding litter quality and house climate were analysed. Samples were taken at day 0 (housing of animals), day 14, and day 30. At the end of the fattening period Salmonella Paratyphi B (d-tartrate +), representing a common strain in German broiler flocks, were isolated from culture. According to the various types of bedding materials some differences in the frequency of Salmonella detection were found. With three samples out of six samples being positive, wood shavings showed the highest Salmonella prevalence, followed by peat with two positive results. Corn silage was found to be a suitable alternative for common litter materials and revealed only one positive sample. Chopped straw was found to be free of Salmonella in our study. However, the latter had a higher risk for negative side effects concerning animal health and house climate. The study showed that the choice of an appropriate litter material might be of considerable importance in order to decrease the Salmonella burden within poultry flocks."],["dc.identifier.doi","10.2376/0005-9366-124-71"],["dc.identifier.isi","000286129500010"],["dc.identifier.pmid","21306057"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24263"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Schluetersche Verlagsgesellschaft Mbh & Co Kg"],["dc.relation.issn","0005-9366"],["dc.title","Frequency of Salmonella detection in a broiler flock depending on different litter materials - a field study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","407"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","TIERAERZTLICHE PRAXIS AUSGABE GROSSTIERE NUTZTIERE"],["dc.bibliographiccitation.lastpage","412"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Gass-Cofre, A."],["dc.contributor.author","Diesterbeck, Ulrike S"],["dc.contributor.author","Urstadt, S."],["dc.contributor.author","Voelkel, Inger"],["dc.contributor.author","Labitzke, T."],["dc.contributor.author","Kaune, Kjell Matthias"],["dc.contributor.author","Czerny, C.-P"],["dc.date.accessioned","2018-11-07T11:20:51Z"],["dc.date.available","2018-11-07T11:20:51Z"],["dc.date.issued","2008"],["dc.description.abstract","Objective: In a pig breeding farm in Lower Bavaria six cases of proliferative dermatitis occurred between August and September 2006 in one to three-week-old suckling pigs from two gilt litters. Materials and methods: Diagnosis and differential diagnosis were confirmed by clinical dermatological examinations, cultural agent detection, electron microscopy, ELISA, and PCR techniques. Results: Clinical symptoms were characterized by multifocal circular exalted and partly fluctuating skin lesions. They aroused suspicion of a poxvirus infection. It was confirmed by the detection of typical virus particles intransmission electron microscopy. The agents of the disease were identified as original Suipoxvirus by polymerase chain reaction (PCR). Orthopoxviruses could be excluded by PCR, a differentiating ELISA, and the replication behavior of the virus isolates in cell cultures. Conclusions and clinical relevance:, Suipoxvirus infections are diagnosed sporadically. The infectious agent is distributed endemically in the environment. New molecular biological techniques enable a genus- and species-specific identification of the poxvirus particles even within parallel and derivative bacterial infections."],["dc.identifier.isi","000262544700007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55637"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Schattauer Gmbh-verlag Medizin Naturwissenschaften"],["dc.relation.issn","1434-1220"],["dc.title","Suipoxvirus infections in suckling piglets"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]