Kirchhoff, Frank

[["dc.bibliographiccitation.firstpage","9836"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.lastpage","9844"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Iafrate, A. John"],["dc.contributor.author","Carl, Silke"],["dc.contributor.author","Bronson, Scott"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Swigut, Tomek"],["dc.contributor.author","Skowronski, Jacek"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2022-10-06T13:25:42Z"],["dc.date.available","2022-10-06T13:25:42Z"],["dc.date.issued","2000"],["dc.description.abstract","ABSTRACT\n The multifunctional simian and human immunodeficiency virus (SIV and HIV) Nef proteins are important for virulence. We studied the importance of selected Nef functions using an SIV Nef with mutations in two regions that are required for CD4 downregulation. This Nef mutant is defective for downregulating CD4 and, in addition, for enhancing SIV infectivity and induction of SIV replication from infected quiescent peripheral blood mononuclear cells, but not for other known functions, including downregulation of class I major histocompatibility complex (MHC) cell surface expression. Replication of SIV containing this Nef variant in rhesus monkeys was attenuated early during infection. Subsequent increases in viral load coincided with selection of reversions and second-site compensatory changes in Nef. Our results indicate that the surfaces of Nef that mediate CD4 downregulation and the enhancement of virion infectivity are critical for SIV replication in vivo. Furthermore, these findings indicate that class I MHC downregulation by Nef is not sufficient for SIV virulence early in infection."],["dc.identifier.doi","10.1128/JVI.74.21.9836-9844.2000"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114896"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://journals.asm.org/non-commercial-tdm-license"],["dc.title","Disrupting Surfaces of Nef Required for Downregulation of CD4 and for Enhancement of Virion Infectivity Attenuates Simian Immunodeficiency Virus Replication In Vivo"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","253"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Medical Primatology"],["dc.bibliographiccitation.lastpage","262"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Klippert, Antonina"],["dc.contributor.author","Stolte-Leeb, Nicole"],["dc.contributor.author","Neumann, Berit"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Daskalaki, Maria"],["dc.contributor.author","Gawanbacht, Ali"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2021-06-01T10:47:23Z"],["dc.date.available","2021-06-01T10:47:23Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1111/jmp.12186"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85587"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0047-2565"],["dc.title","Frequencies of lymphoid T-follicular helper cells obtained longitudinally by lymph node fine-needle aspiration correlate significantly with viral load in SIV-infected rhesus monkeys"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","267"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Virology"],["dc.bibliographiccitation.lastpage","272"],["dc.bibliographiccitation.volume","183"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Voss, Gerald"],["dc.contributor.author","Nick, Sigrid"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Coulibaly, Cheick"],["dc.contributor.author","Frank, Ronald"],["dc.contributor.author","Jentsch, Klaus-Dieter"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.date.accessioned","2022-10-06T13:32:39Z"],["dc.date.available","2022-10-06T13:32:39Z"],["dc.date.issued","1991"],["dc.identifier.doi","10.1016/0042-6822(91)90139-3"],["dc.identifier.pii","0042682291901393"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115433"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0042-6822"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Antibody response to the negative regulatory factor (nef) in experimentally infected macaques: Correlation with viremia, disease progression, and seroconversion to structural viral proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1371"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.lastpage","16"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Joas, Simone"],["dc.contributor.author","Parrish, Erica H."],["dc.contributor.author","Gnanadurai, Clement W."],["dc.contributor.author","Lump, Edina"],["dc.contributor.author","Stürzel, Christina M."],["dc.contributor.author","Parrish, Nicholas F."],["dc.contributor.author","Learn, Gerald H."],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Neumann, Berit"],["dc.contributor.author","Rensing, Kerstin Mätz"],["dc.contributor.author","Fuchs, Dietmar"],["dc.contributor.author","Billingsley, James M."],["dc.contributor.author","Bosinger, Steven E."],["dc.contributor.author","Silvestri, Guido"],["dc.contributor.author","Apetrei, Cristian"],["dc.contributor.author","Huot, Nicolas"],["dc.contributor.author","Garcia-Tellez, Thalia"],["dc.contributor.author","Müller-Trutwin, Michaela"],["dc.contributor.author","Hotter, Dominik"],["dc.contributor.author","Sauter, Daniel"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Hahn, Beatrice H."],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2018-11-15T12:52:42Z"],["dc.date.accessioned","2021-10-27T13:13:05Z"],["dc.date.available","2018-11-15T12:52:42Z"],["dc.date.available","2021-10-27T13:13:05Z"],["dc.date.issued","2018"],["dc.description.abstract","HIV-1 causes chronic inflammation and AIDS in humans, whereas related simian immunodeficiency viruses (SIVs) replicate efficiently in their natural hosts without causing disease. It is currently unknown to what extent virus-specific properties are responsible for these different clinical outcomes. Here, we incorporate two putative HIV-1 virulence determinants, i.e., a Vpu protein that antagonizes tetherin and blocks NF-κB activation and a Nef protein that fails to suppress T cell activation via downmodulation of CD3, into a non-pathogenic SIVagm strain and test their impact on viral replication and pathogenicity in African green monkeys. Despite sustained high-level viremia over more than 4 years, moderately increased immune activation and transcriptional signatures of inflammation, the HIV-1-like SIVagm does not cause immunodeficiency or any other disease. These data indicate that species-specific host factors rather than intrinsic viral virulence factors determine the pathogenicity of primate lentiviruses."],["dc.identifier.doi","10.1038/s41467-018-03762-3"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15592"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91749"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2041-1723"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4469"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.lastpage","4481"],["dc.bibliographiccitation.volume","80"],["dc.contributor.author","Brenner, Matthias"],["dc.contributor.author","Münch, Jan"],["dc.contributor.author","Schindler, Michael"],["dc.contributor.author","Wildum, Steffen"],["dc.contributor.author","Stolte, Nicole"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Fuchs, Dietmar"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Heeney, Jonathan"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2022-10-06T13:25:46Z"],["dc.date.available","2022-10-06T13:25:46Z"],["dc.date.issued","2006"],["dc.description.abstract","ABSTRACT\n \n Point mutations in SIVmac239 Nef disrupting CD4 downmodulation and enhancement of virion infectivity attenuate viral replication in acutely infected rhesus macaques, but changes selected later in infection fully restore Nef function (A. J. Iafrate et al., J. Virol. 74:9836-9844, 2000). To further evaluate the relevance of these Nef functions for viral persistence and disease progression, we analyzed an SIVmac239 Nef mutant containing a deletion of amino acids Q64 to N67 (Δ64-67Nef). This mutation inactivates the N-distal AP-2 clathrin adaptor binding element and disrupts the abilities of Nef to downregulate CD4, CD28 and CXCR4 and to stimulate viral replication in vitro. However, it does not impair the downmodulation of CD3 and class I major histocompatibility complex (MHC-I) or MHC-II and the upregulation of the MHC-II-associated invariant chain, and it has only a moderate effect on the enhancement of virion infectivity. Replication of the Δ64-67Nef variant in acutely infected macaques was intermediate between grossly\n nef\n -deleted and wild-type SIVmac239. Subsequently, three of six macaques developed moderate to high viral loads and developed disease, whereas the remaining animals efficiently controlled SIV replication and showed a more attenuated clinical course of infection. Sequence analysis revealed that the deletion in\n nef\n was not repaired in any of these animals. However, some changes that slightly enhanced the ability of Nef to downmodulate CD4 and moderately increased Nef-mediated enhancement of viral replication and infectivity in vitro were observed in macaques developing high viral loads. Our results imply that both the Nef functions that were disrupted by the Δ64-67 mutation and the activities that remained intact contribute to viral pathogenicity."],["dc.identifier.doi","10.1128/JVI.80.9.4469-4481.2006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114910"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://journals.asm.org/non-commercial-tdm-license"],["dc.title","Importance of the N-Distal AP-2 Binding Element in Nef for Simian Immunodeficiency Virus Replication and Pathogenicity in Rhesus Macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Retrovirology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Münch, Jan"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Yolamanova, Maral"],["dc.contributor.author","Raue, Katharina"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2022-10-06T13:26:10Z"],["dc.date.available","2022-10-06T13:26:10Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1186/1742-4690-10-148"],["dc.identifier.pii","3638"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115017"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1742-4690"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Effect of semen and seminal amyloid on vaginal transmission of simian immunodeficiency virus"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8371"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.lastpage","8383"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Münch, Jan"],["dc.contributor.author","Carl, Silke"],["dc.contributor.author","Stolte, Nicole"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Fuchs, Dietmar"],["dc.contributor.author","Ten Haaft, Peter"],["dc.contributor.author","Heeney, Jonathan L."],["dc.contributor.author","Swigut, Tomek"],["dc.contributor.author","Skowronski, Jacek"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2022-10-06T13:25:42Z"],["dc.date.available","2022-10-06T13:25:42Z"],["dc.date.issued","1999"],["dc.description.abstract","ABSTRACT\n \n The\n nef\n gene of the pathogenic simian immunodeficiency virus (SIV) 239 clone was replaced with primary human immunodeficiency virus type 1 (HIV-1)\n nef\n alleles to investigate whether HIV-1 Nef can substitute for SIV Nef in vivo. Initially, two rhesus macaques were infected with the chimeric viruses (Nef-SHIVs). Most of the\n nef\n alleles obtained from both animals predicted intact open reading frames. Furthermore, forms containing upstream nucleotide substitutions that enhanced expression of the inserted gene became predominant. One animal maintained high viral loads and slowly progressed to immunodeficiency.\n nef\n long terminal repeat sequences amplified from this animal were used to generate a second generation of Nef-SHIVs. Two macaques, which were subsequently infected with a mixture of cloned chimeric viruses, showed high viral loads and progressed to fatal immunodeficiency. Five macaques received a single molecular clone, named SHIV-40K6. The SHIV-40K6\n nef\n allele was active in CD4 and class I major histocompatibility complex downregulation and enhanced viral infectivity and replication. Notably, all of the macaques inoculated with SHIV-40K6 showed high levels of viral replication early in infection. During later stages, however, the course of infection was variable. Three animals maintained high viral loads and developed immunodeficiency. Of the remaining two macaques, which showed decreasing viral loads after the acute phase of infection, only one efficiently controlled viral replication and remained asymptomatic during 1.5 years of follow-up. The other animal showed an increasing viral load and developed signs of progressive infection during later stages. Our data demonstrate that HIV-1\n nef\n can, to a large extent, functionally replace SIVmac\n nef\n in vivo."],["dc.identifier.doi","10.1128/JVI.73.10.8371-8383.1999"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114894"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://journals.asm.org/non-commercial-tdm-license"],["dc.title","The Human Immunodeficiency Virus Type 1\n nef\n Gene Can to a Large Extent Replace Simian Immunodeficiency Virus\n nef\n In Vivo"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","860"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Medicine"],["dc.bibliographiccitation.lastpage","865"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Lang, Sabine M."],["dc.contributor.author","Lafrate, A. John"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Kuhn, Eva M."],["dc.contributor.author","Nisslein, Thomas"],["dc.contributor.author","Kaup, Franz-Josef"],["dc.contributor.author","Haupt, Marianne"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Skowronski, Jacek"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2022-10-06T13:34:12Z"],["dc.date.available","2022-10-06T13:34:12Z"],["dc.date.issued","1997"],["dc.identifier.doi","10.1038/nm0897-860"],["dc.identifier.pii","BFnm0897860"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115855"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1546-170X"],["dc.relation.issn","1078-8956"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","455"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","AIDS"],["dc.bibliographiccitation.lastpage","458"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Schneider, Josef"],["dc.contributor.author","Lüke, Wolfgang"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Jung, Roswitha"],["dc.contributor.author","Jurkiewicz, Elke"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Nick, Sigrid"],["dc.contributor.author","Klemm, Edzard"],["dc.contributor.author","Jentsch, Klaus-Dieter"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.date.accessioned","2022-10-06T13:35:27Z"],["dc.date.available","2022-10-06T13:35:27Z"],["dc.date.issued","1990"],["dc.identifier.doi","10.1097/00002030-199005000-00012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116101"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0269-9370"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Isolation and characterization of HIV-2ben obtained from a patient with predominantly neurological defects"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","61"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Virology"],["dc.bibliographiccitation.lastpage","70"],["dc.bibliographiccitation.volume","254"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Carl, Silke"],["dc.contributor.author","Sopper, Sieghart"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2022-10-06T13:26:58Z"],["dc.date.available","2022-10-06T13:26:58Z"],["dc.date.issued","1999"],["dc.identifier.doi","10.1006/viro.1998.9522"],["dc.identifier.pii","S0042682298995228"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115213"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0042-6822"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Selection of the R17Y Substitution in SIVmac239 Nef Coincided with a Dramatic Increase in Plasma Viremia and Rapid Progression to Death"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]