Pech, Ulrike

[["dc.bibliographiccitation.firstpage","952"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","960"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Issa, Abdul-Raouf"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Coulom, Helene"],["dc.contributor.author","My-Van Nguyen, My-Van Nguyen"],["dc.contributor.author","Cassar, Marlene"],["dc.contributor.author","Jacquet, Melanie"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Birman, Serge"],["dc.date.accessioned","2018-11-07T09:17:49Z"],["dc.date.available","2018-11-07T09:17:49Z"],["dc.date.issued","2013"],["dc.description.abstract","Expression of the human Parkinson-disease-associated protein alpha-synuclein in all Drosophila neurons induces progressive locomotor deficits. Here, we identify a group of 15 dopaminergic neurons per hemisphere in the anterior medial region of the brain whose disruption correlates with climbing impairments in this model. These neurons selectively innervate the horizontal beta and beta' lobes of the mushroom bodies, and their connections to the Kenyon cells are markedly reduced when they express alpha-synuclein. Using selective mushroom body drivers, we show that blocking or overstimulating neuronal activity in the beta' lobe, but not the beta or gamma lobes, significantly inhibits negative geotaxis behavior. This suggests that modulation of the mushroom body beta' lobes by this dopaminergic pathway is specifically required for an efficient control of startle-induced locomotion in flies."],["dc.identifier.doi","10.1016/j.celrep.2013.10.032"],["dc.identifier.isi","000328266000011"],["dc.identifier.pmid","24239353"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10671"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28258"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","2211-1247"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","A Single Dopamine Pathway Underlies Progressive Locomotor Deficits in a Drosophila Model of Parkinson Disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Maglione, Marta"],["dc.contributor.author","Schubert, Marco"],["dc.contributor.author","Matkovic-Rachid, Tanja"],["dc.contributor.author","Hasheminasab, Sayed-mohammad"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Fiala, André"],["dc.contributor.author","Mielke, Thorsten"],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2021-06-01T09:41:40Z"],["dc.date.available","2021-06-01T09:41:40Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The physical distance between presynaptic Ca 2+ channels and the Ca 2+ sensors triggering the release of neurotransmitter-containing vesicles regulates short-term plasticity (STP). While STP is highly diversified across synapse types, the computational and behavioral relevance of this diversity remains unclear. In the Drosophila brain, at nanoscale level, we can distinguish distinct coupling distances between Ca 2+ channels and the (m)unc13 family priming factors, Unc13A and Unc13B. Importantly, coupling distance defines release components with distinct STP characteristics. Here, we show that while Unc13A and Unc13B both contribute to synaptic signalling, they play distinct roles in neural decoding of olfactory information at excitatory projection neuron (ePN) output synapses. Unc13A clusters closer to Ca 2+ channels than Unc13B, specifically promoting fast phasic signal transfer. Reduction of Unc13A in ePNs attenuates responses to both aversive and appetitive stimuli, while reduction of Unc13B provokes a general shift towards appetitive values. Collectively, we provide direct genetic evidence that release components of distinct nanoscopic coupling distances differentially control STP to play distinct roles in neural decoding of sensory information."],["dc.identifier.doi","10.1038/s41467-021-22180-6"],["dc.identifier.pmid","33771998"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84997"],["dc.identifier.url","https://for2705.de/literature/publications/38"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","FOR 2705: Dissection of a Brain Circuit: Structure, Plasticity and Behavioral Function of the Drosophila Mushroom Body"],["dc.relation","FOR 2705 | TP 5: Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies"],["dc.relation.eissn","2041-1723"],["dc.relation.workinggroup","RG Fiala"],["dc.relation.workinggroup","RG Sigrist (Genetics)"],["dc.rights","CC BY 4.0"],["dc.title","Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2083"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","2095"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Revelo, Natalia H."],["dc.contributor.author","Seitz, Katharina J."],["dc.contributor.author","Rizzoli, S. O."],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2017-09-07T11:44:29Z"],["dc.date.available","2017-09-07T11:44:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly strains that express functional fluorescent reporters targeted to either pre-or postsynaptic compartments. Presynaptic Ca2+ dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission is monitored using red fluorescent synaptophysinpHTomato, and postsynaptic Ca2+ dynamics are visualized usingGCaMP3fused with the postsynaptic matrix protein, dHomer. Using two-photon in vivo imaging of olfactory projection neurons, odor-evoked activity across populations of synapses is visualized in the antennal lobe and the mushroom body calyx. Prolonged odor exposure causes odor-specific and differential experience-dependent changes in preand postsynaptic activity at both levels of olfactory processing. The approach advances the physiological analysis of synaptic connections across defined groups of neurons in intact Drosophila."],["dc.identifier.doi","10.1016/j.celrep.2015.02.065"],["dc.identifier.gro","3141936"],["dc.identifier.isi","000352138400014"],["dc.identifier.pmid","25818295"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2724"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2211-1247"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","147"],["dc.bibliographiccitation.journal","Frontiers in Neural Circuits"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Dipt, Shubham"],["dc.contributor.author","Barth, Jonas"],["dc.contributor.author","Singh, Priyanka"],["dc.contributor.author","Jauch, Mandy"],["dc.contributor.author","Thum, Andreas S."],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Riemensperger, Thomas"],["dc.date.accessioned","2018-11-07T09:19:49Z"],["dc.date.available","2018-11-07T09:19:49Z"],["dc.date.issued","2013"],["dc.description.abstract","The fruit fly Drosophila melanogaster represents a key model organism for analyzing how neuronal circuits regulate behavior. The mushroom body in the central brain is a particularly prominent brain region that has been intensely studied in several insect species and been implicated in a variety of behaviors, e.g., associative learning, locomotor activity, and sleep. Drosophila melanogaster offers the advantage that transgenes can be easily expressed in neuronal subpopulations, e.g., in intrinsic mushroom body neurons (Kenyon cells). A number of transgenes has been described and engineered to visualize the anatomy of neurons, to monitor physiological parameters of neuronal activity, and to manipulate neuronal function artificially. To target the expression of these transgenes selectively to specific neurons several sophisticated bi- or even multipartite transcription systems have been invented. However, the number of transgenes that can be combined in the genome of an individual fly is limited in practice. To facilitate the analysis of the mushroom body we provide a compilation of transgenic fruit flies that express transgenes under direct control of the Kenyon-cell specific promoter, mb247. The transgenes expressed are fluorescence reporters to analyze neuroanatomical aspects of the mushroom body, proteins to restrict ectopic gene expression to mushroom bodies, or fluorescent sensors to monitor physiological parameters of neuronal activity of Kenyon cells. Some of the transgenic animals compiled here have been published already, whereas others are novel and characterized here for the first time. Overall, the collection of transgenic flies expressing sensor and reporter genes in Kenyon cells facilitates combinations with binary transcription systems and might, ultimately, advance the physiological analysis of mushroom body function."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.3389/fncir.2013.00147"],["dc.identifier.isi","000324807300001"],["dc.identifier.pmid","24065891"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9305"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28729"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Research Foundation"],["dc.relation.issn","1662-5110"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Mushroom body miscellanea: transgenic Drosophila strains expressing anatomical and physiological sensor proteins in Kenyon cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1002563"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS Biology"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Gupta, Varun K."],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Bhukel, Anuradha"],["dc.contributor.author","Fulterer, Andreas"],["dc.contributor.author","Ender, Anatoli"],["dc.contributor.author","Mauermann, Stephan F."],["dc.contributor.author","Andlauer, Till F. M."],["dc.contributor.author","Antwi-Adjei, Emmanuel"],["dc.contributor.author","Beuschel, Christine"],["dc.contributor.author","Thriene, Kerstin"],["dc.contributor.author","Maglione, Marta"],["dc.contributor.author","Quentin, Christine"],["dc.contributor.author","Bushow, Rene"],["dc.contributor.author","Schwaerzel, Martin"],["dc.contributor.author","Mielke, Thorsten"],["dc.contributor.author","Madeo, Frank"],["dc.contributor.author","Dengjel, Joern"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2018-11-07T10:09:04Z"],["dc.date.available","2018-11-07T10:09:04Z"],["dc.date.issued","2016"],["dc.description.abstract","Memories are assumed to be formed by sets of synapses changing their structural or functional performance. The efficacy of forming new memories declines with advancing age, but the synaptic changes underlying age-induced memory impairment remain poorly understood. Recently, we found spermidine feeding to specifically suppress age-dependent impairments in forming olfactory memories, providing a mean to search for synaptic changes involved in age-dependent memory impairment. Here, we show that a specific synaptic compartment, the presynaptic active zone (AZ), increases the size of its ultrastructural elaboration and releases significantly more synaptic vesicles with advancing age. These age-induced AZ changes, however, were fully suppressed by spermidine feeding. A genetically enforced enlargement of AZ scaffolds (four gene-copies of BRP) impaired memory formation in young animals. Thus, in the Drosophila nervous system, aging AZs seem to steer towards the upper limit of their operational range, limiting synaptic plasticity and contributing to impairment of memory formation. Spermidine feeding suppresses age-dependent memory impairment by counteracting these age-dependent changes directly at the synapse."],["dc.identifier.doi","10.1371/journal.pbio.1002563"],["dc.identifier.isi","000386128900019"],["dc.identifier.pmid","27684064"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13761"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39590"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1545-7885"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]