Boretius, Susann

[["dc.bibliographiccitation.artnumber","119730"],["dc.bibliographiccitation.firstpage","119730"],["dc.bibliographiccitation.journal","NeuroImage"],["dc.bibliographiccitation.volume","264"],["dc.contributor.author","Dadarwal, Rakshit"],["dc.contributor.author","Ortiz-Rios, Michael"],["dc.contributor.author","Boretius, Susann"],["dc.date.accessioned","2022-12-01T08:30:41Z"],["dc.date.available","2022-12-01T08:30:41Z"],["dc.date.issued","2022"],["dc.description.abstract","Recent progress in quantitative susceptibility mapping (QSM) has enabled the accurate delineation of submillimeter-scale subcortical brain structures in humans. However, the simultaneous visualization of cortical, subcortical, and white matter structure remains challenging, utilizing QSM data solely. Here we present TQ-SILiCON, a fusion method that enhances the contrast of cortex and subcortical structures and provides an excellent white matter delineation by combining QSM and conventional T1-weighted (T1w) images. In this study, we first applied QSM in the macaque monkey to map iron-rich subcortical structures. Implementing the same QSM acquisition and analysis methods allowed a similar accurate delineation of subcortical structures in humans. However, the QSM contrast of white and cortical gray matter was not sufficient for appropriate segmentation. Applying automatic brain tissue segmentation to TQ-SILiCON images of the macaque improved the classification of subcortical brain structures as compared to the single T1 contrast by maintaining an excellent white to cortical gray matter contrast. Furthermore, we validated our dual-contrast fusion approach in humans and similarly demonstrated improvements in automated segmentation of the cortex and subcortical structures. We believe the proposed contrast will facilitate translational studies in nonhuman primates to investigate the pathophysiology of neurodegenerative diseases that affect subcortical structures such as the basal ganglia in humans."],["dc.identifier.doi","10.1016/j.neuroimage.2022.119730"],["dc.identifier.pii","S1053811922008515"],["dc.identifier.pmid","36332851"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117952"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1095-9572"],["dc.relation.issn","1053-8119"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Fusion of quantitative susceptibility maps and T1-weighted images improve brain tissue contrast in primates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1427"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","1443"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Hafner, Georg"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Truschow, Pavel"],["dc.contributor.author","Rüppel, Alina"],["dc.contributor.author","Sirmpilatze, Nikoloz"],["dc.contributor.author","Dadarwal, Rakshit"],["dc.contributor.author","Boretius, Susann"],["dc.contributor.author","Staiger, Jochen F"],["dc.date.accessioned","2021-06-01T09:41:52Z"],["dc.date.available","2021-06-01T09:41:52Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The neocortex is composed of layers. Whether layers constitute an essential framework for the formation of functional circuits is not well understood. We investigated the brain-wide input connectivity of vasoactive intestinal polypeptide (VIP) expressing neurons in the reeler mouse. This mutant is characterized by a migration deficit of cortical neurons so that no layers are formed. Still, neurons retain their properties and reeler mice show little cognitive impairment. We focused on VIP neurons because they are known to receive strong long-range inputs and have a typical laminar bias toward upper layers. In reeler, these neurons are more dispersed across the cortex. We mapped the brain-wide inputs of VIP neurons in barrel cortex of wild-type and reeler mice with rabies virus tracing. Innervation by subcortical inputs was not altered in reeler, in contrast to the cortical circuitry. Numbers of long-range ipsilateral cortical inputs were reduced in reeler, while contralateral inputs were strongly increased. Reeler mice had more callosal projection neurons. Hence, the corpus callosum was larger in reeler as shown by structural imaging. We argue that, in the absence of cortical layers, circuits with subcortical structures are maintained but cortical neurons establish a different network that largely preserves cognitive functions."],["dc.identifier.doi","10.1093/cercor/bhaa280"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85068"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.title","Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","121"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Acta Neuropathologica Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Wilke, Justus B. H."],["dc.contributor.author","Hindermann, Martin"],["dc.contributor.author","Moussavi, Amir"],["dc.contributor.author","Butt, Umer J."],["dc.contributor.author","Dadarwal, Rakshit"],["dc.contributor.author","Berghoff, Stefan A."],["dc.contributor.author","Sarcheshmeh, Aref K."],["dc.contributor.author","Ronnenberg, Anja"],["dc.contributor.author","Zihsler, Svenja"],["dc.contributor.author","Arinrad, Sahab"],["dc.contributor.author","Hardeland, Rüdiger"],["dc.contributor.author","Seidel, Jan"],["dc.contributor.author","Lühder, Fred"],["dc.contributor.author","Nave, Klaus-Armin"],["dc.contributor.author","Boretius, Susann"],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.date.accessioned","2021-11-25T11:24:44Z"],["dc.date.accessioned","2022-08-18T12:41:08Z"],["dc.date.available","2021-11-25T11:24:44Z"],["dc.date.available","2022-08-18T12:41:08Z"],["dc.date.issued","2021-07-02"],["dc.date.updated","2022-07-29T12:18:38Z"],["dc.description.abstract","Abstract\r\n Up to one person in a population of 10,000 is diagnosed once in lifetime with an encephalitis, in 50–70% of unknown origin. Recognized causes amount to 20–50% viral infections. Approximately one third of affected subjects develops moderate and severe subsequent damage. Several neurotropic viruses can directly infect pyramidal neurons and induce neuronal death in cortex and hippocampus. The resulting encephalitic syndromes are frequently associated with cognitive deterioration and dementia, but involve numerous parallel and downstream cellular and molecular events that make the interpretation of direct consequences of sudden pyramidal neuronal loss difficult. This, however, would be pivotal for understanding how neuroinflammatory processes initiate the development of neurodegeneration, and thus for targeted prophylactic and therapeutic interventions. Here we utilized adult male NexCreERT2xRosa26-eGFP-DTA (= ‘DTA’) mice for the induction of a sterile encephalitis by diphtheria toxin-mediated ablation of cortical and hippocampal pyramidal neurons which also recruits immune cells into gray matter. We report multifaceted aftereffects of this defined process, including the expected pathology of classical hippocampal behaviors, evaluated in Morris water maze, but also of (pre)frontal circuit function, assessed by prepulse inhibition. Importantly, we modelled in encephalitis mice novel translationally relevant sequelae, namely altered social interaction/cognition, accompanied by compromised thermoreaction to social stimuli as convenient readout of parallel autonomic nervous system (dys)function. High resolution magnetic resonance imaging disclosed distinct abnormalities in brain dimensions, including cortical and hippocampal layering, as well as of cerebral blood flow and volume. Fluorescent tracer injection, immunohistochemistry and brain flow cytometry revealed persistent blood–brain-barrier perturbance and chronic brain inflammation. Surprisingly, blood flow cytometry showed no abnormalities in circulating major immune cell subsets and plasma high-mobility group box 1 (HMGB1) as proinflammatory marker remained unchanged. The present experimental work, analyzing multidimensional outcomes of direct pyramidal neuronal loss, will open new avenues for urgently needed encephalitis research."],["dc.identifier.citation","Acta Neuropathologica Communications. 2021 Jul 02;9(1):121"],["dc.identifier.doi","10.1186/s40478-021-01214-6"],["dc.identifier.pii","1214"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/93550"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112989"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.publisher","BioMed Central"],["dc.relation.eissn","2051-5960"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","Diphtheria toxin"],["dc.subject","Hippocampal learning and memory"],["dc.subject","(pre)frontal network dysfunction"],["dc.subject","Social cognition"],["dc.subject","Thermography"],["dc.subject","Magnetic resonance imaging"],["dc.title","Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]