Geumann, Constanze

[["dc.bibliographiccitation.firstpage","3477"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.lastpage","3487"],["dc.bibliographiccitation.volume","127"],["dc.contributor.author","Baltes, Jennifer"],["dc.contributor.author","Larsen, Jakob Vejby"],["dc.contributor.author","Radhakrishnan, Karthikeyan"],["dc.contributor.author","Geumann, Constanze"],["dc.contributor.author","Kratzke, Manuel"],["dc.contributor.author","Petersen, Claus Munck"],["dc.contributor.author","Schu, Peter"],["dc.date.accessioned","2018-11-07T09:36:33Z"],["dc.date.available","2018-11-07T09:36:33Z"],["dc.date.issued","2014"],["dc.description.abstract","Here, we describe altered sorting of sortilin in adipocytes deficient for the sigma 1B-containing AP-1 complex, leading to the inhibition of adipogenesis. The AP-1 complex mediates protein sorting between the trans-Golgi network and endosomes. Vertebrates express three AP1 sigma 1 subunit isoforms - sigma 1A, sigma 1B and sigma 1C (also known as AP1S1, AP1S2 and AP1S3, respectively). sigma 1B-deficient mice display impaired recycling of synaptic vesicles and lipodystrophy. Here, we show that sortilin is overexpressed in adipose tissue from sigma 1B(-/-) mice, and that its overexpression in wild-type cells is sufficient to suppress adipogenesis. sigma 1B-specific binding of sortilin requires the sortilin DxxD-x12-DSxxxL motif. sigma 1B deficiency does not lead to a block of sortilin transport out of a specific organelle, but the fraction that reaches lysosomes is reduced. Sortilin binds to the receptor DLK1, an inhibitor of adipocyte differentiation, and the overexpression of sortilin prevents DLK1 downregulation, leading to enhanced inhibition of adipogenesis. DLK1 and sortilin expression are not increased in the brain tissue of sigma 1B(-/-) mice, although this is the tissue with the highest expression of sigma 1B and sortilin. Thus, adipose-tissue-specific and sigma 1B-dependent routes for the transport of sortilin exist and are involved in the regulation of adipogenesis and adipose-tissue mass."],["dc.identifier.doi","10.1242/jcs.146886"],["dc.identifier.isi","000341180000008"],["dc.identifier.pmid","24928897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32644"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","1477-9137"],["dc.relation.issn","0021-9533"],["dc.title","sigma 1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1318"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.lastpage","1330"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Glyvuk, Nataliya"],["dc.contributor.author","Tsytsyura, Yaroslav"],["dc.contributor.author","Geumann, Constanze"],["dc.contributor.author","D'Hooge, Rudi"],["dc.contributor.author","Hueve, Jana"],["dc.contributor.author","Kratzke, Manuel"],["dc.contributor.author","Baltes, Jennifer"],["dc.contributor.author","Boening, Daniel"],["dc.contributor.author","Klingauf, Juergen"],["dc.contributor.author","Schu, Peter"],["dc.date.accessioned","2018-11-07T08:43:59Z"],["dc.date.available","2018-11-07T08:43:59Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicle recycling involves AP-2/clathrin-mediated endocytosis, but it is not known whether the endosomal pathway is also required. Mice deficient in the tissue-specific AP-1-sigma 1B complex have impaired synaptic vesicle recycling in hippocampal synapses. The ubiquitously expressed AP-1-sigma 1A complex mediates protein sorting between the trans-Golgi network and early endosomes. Vertebrates express three sigma 1 subunit isoforms: A, B and C. The expressions of sigma 1A and sigma 1B are highest in the brain. Synaptic vesicle reformation in cultured neurons from sigma 1B-deficient mice is reduced upon stimulation, and large endosomal intermediates accumulate. The sigma 1B-deficient mice have reduced motor coordination and severely impaired long-term spatial memory. These data reveal a molecular mechanism for a severe human X-chromosome-linked mental retardation. The EMBO Journal (2010) 29, 1318-1330. doi: 10.1038/emboj.2010.15; Published online 4 March 2010"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SFB 523 A6, SFB 523 B10]"],["dc.identifier.doi","10.1038/emboj.2010.15"],["dc.identifier.isi","000276927000003"],["dc.identifier.pmid","20203623"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20103"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0261-4189"],["dc.title","AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","356"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","368"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Zizioli, Daniela"],["dc.contributor.author","Geumann, Constanze"],["dc.contributor.author","Kratzke, Manuel"],["dc.contributor.author","Mishra, Ratnakar"],["dc.contributor.author","Borsani, Guiseppe"],["dc.contributor.author","Finazzi, Dario"],["dc.contributor.author","Candiello, Ermes"],["dc.contributor.author","Schu, Peter"],["dc.date.accessioned","2018-11-07T10:22:53Z"],["dc.date.available","2018-11-07T10:22:53Z"],["dc.date.issued","2017"],["dc.description.abstract","gamma 2 adaptin is homologous to gamma 1, but is only expressed in vertebrates while gamma 1 is found in all eukaryotes. We know little about gamma 2 functions and their relation to gamma 1. gamma 1 is an adaptin of the heterotetrameric AP-1 complexes, which sort proteins in and do form clathrin-coated transport vesicles and they also regulate maturation of early endosomes. gamma 1 knockout mice develop only to blastocysts and thus gamma 2 does not compensate gamma 1-deficiency in development. gamma 2 has not been classified as a clathrin-coated vesicle adaptor protein in proteome analyses and functions for monomeric gamma 2 in endosomal protein sorting have been proposed, but adaptin interaction studies suggested formation of heterotetrameric AP-1/gamma 2 complexes. We detected gamma 2 at the trans-Golgi network, on peripheral vesicles and identified gamma 2 clathrin-coated vesicles in mice. Ubiquitous sigma 1A and tissue-specific sigma 1B adaptins bind gamma 2 and gamma 1. sigma 1B knockout in mice does not effect gamma 1/sigma 1A AP-1 levels, but gamma 2/sigma 1A AP-1 levels are increased in brain and adipocytes. Also gamma 2 is essential in development. In zebrafish AP-1/gamma 2 and AP-1/gamma 1 fulfill different, essential functions in brain and the vascular system. (C) 2017 Elsevier GmbH. All rights reserved."],["dc.identifier.doi","10.1016/j.ejcb.2017.03.008"],["dc.identifier.isi","000404504700007"],["dc.identifier.pmid","28372831"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42353"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","1618-1298"],["dc.relation.issn","0171-9335"],["dc.title","gamma 2 and gamma 1AP-1 complexes: Different essential functions and regulatory mechanisms in clathrin-dependent protein sorting"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Glyvuk, Nataliya"],["dc.contributor.author","Tsytsyura, Yaroslav"],["dc.contributor.author","Geumann, Constanze"],["dc.contributor.author","D'Hooge, Rudi"],["dc.contributor.author","Hueve, Jana"],["dc.contributor.author","Kratzke, Manuel"],["dc.contributor.author","Baltes, Jennifer"],["dc.contributor.author","Boening, Daniel"],["dc.contributor.author","Klingauf, Juergen"],["dc.contributor.author","Schu, Peter"],["dc.date.accessioned","2018-11-07T08:42:20Z"],["dc.date.available","2018-11-07T08:42:20Z"],["dc.date.issued","2010"],["dc.format.extent","1941"],["dc.identifier.doi","10.1038/emboj.2010.96"],["dc.identifier.isi","000278235100015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19673"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0261-4189"],["dc.title","AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory (vol 29, pg 1318, 2010)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]