Rehfeldt, Florian

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Harada, T."],["dc.contributor.author","Brown, A. E. X."],["dc.contributor.author","Zajac, Allison L."],["dc.contributor.author","Cai, Shenshen"],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:00:13Z"],["dc.date.available","2018-11-07T09:00:13Z"],["dc.date.issued","2011"],["dc.identifier.isi","000305505504181"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24099"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.conference","Annual Meeting of the American-Society-for-Cell-Biology (ASCB)"],["dc.relation.eventlocation","Denver, CO"],["dc.relation.issn","1059-1524"],["dc.title","Matrix Stiffness Dictates Cytoskeletal Organization and Nuclear Morphology in 2D and 3D."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","422"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Integrative Biology"],["dc.bibliographiccitation.lastpage","430"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Brown, Andre E. X."],["dc.contributor.author","Raab, Matthew"],["dc.contributor.author","Cai, Shenshen"],["dc.contributor.author","Zajac, Allison L."],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:15:30Z"],["dc.date.available","2018-11-07T09:15:30Z"],["dc.date.issued","2012"],["dc.description.abstract","Physical features of microenvironments such as matrix elasticity E can clearly influence cell morphology and cell phenotype, but many differences between model matrices raise questions as to whether a standard biological scale for E exists, especially in 3D as well as in 2D. An E-series of two distinct types of hydrogels are ligand-functionalized here with non-fibrous collagen and used to elucidate wide-ranging cell and cytoskeletal responses to E in both 2D and 3D matrix geometries. Cross-linked hyaluronic acid (HA) based matrices as well as standard polyacrylamide (PA) hydrogels show that, within hours of initial plating, the adhesion, asymmetric shape, and cytoskeletal order within mesenchymal stem cells generally depend on E nonmonotonically over a broad range of physiologically relevant E. In particular, with overlays of a second matrix the stiffer of the upper or lower matrix dominates key cell responses to 3D: the cell invariably takes an elongated shape that couples to E in driving cytoplasmic stress fiber assembly. In contrast, embedding cells in homogeneous HA matrices constrains cells to spherically symmetric shapes in which E drives the assembly of a predominantly cortical cytoskeleton. Non-muscle myosin II generates the forces required for key cell responses and is a target of a phospho-Tyrosine signaling pathway that likely regulates contractile assemblies and also depends nonmonotonically on E. The results can be understood in part from a theory for stress fiber polarization that couples to matrix elasticity as well as cell shape and accurately predicts cytoskeletal order in 2D and 3D, regardless of polymer system."],["dc.identifier.doi","10.1039/c2ib00150k"],["dc.identifier.fs","597311"],["dc.identifier.isi","000302017100009"],["dc.identifier.pmid","22344328"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9576"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27709"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1757-9694"],["dc.relation.orgunit","Fakultät für Physik"],["dc.subject.mesh","Acrylic Resins"],["dc.subject.mesh","Actins"],["dc.subject.mesh","Cell Adhesion"],["dc.subject.mesh","Cell Proliferation"],["dc.subject.mesh","Cell Shape"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Collagen Type I"],["dc.subject.mesh","Elastic Modulus"],["dc.subject.mesh","Elasticity"],["dc.subject.mesh","Extracellular Matrix"],["dc.subject.mesh","Gelatin"],["dc.subject.mesh","Heterocyclic Compounds with 4 or More Rings"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Hyaluronic Acid"],["dc.subject.mesh","Hydrogels"],["dc.subject.mesh","Mesenchymal Stromal Cells"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Microscopy, Fluorescence"],["dc.subject.mesh","Myosin Heavy Chains"],["dc.subject.mesh","Nonmuscle Myosin Type IIA"],["dc.subject.mesh","Phosphorylation"],["dc.subject.mesh","Phosphotyrosine"],["dc.subject.mesh","Stress Fibers"],["dc.subject.mesh","Vinculin"],["dc.title","Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Potyrala, Milena"],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:16:18Z"],["dc.date.available","2018-11-07T09:16:18Z"],["dc.date.issued","2012"],["dc.identifier.isi","000209348606208"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27910"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.title","Matrix Stiffness Controls Nuclear Morphology of Human Mesenchymal Stem Cells in 2D and 3D."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","194110"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Journal of Physics Condensed Matter"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Brown, A. E. X."],["dc.contributor.author","Discher, Dennis E."],["dc.contributor.author","Safran, S. A."],["dc.date.accessioned","2018-11-07T08:43:11Z"],["dc.date.available","2018-11-07T08:43:11Z"],["dc.date.issued","2010"],["dc.description.abstract","The active regulation of cellular forces during cell adhesion plays an important role in the determination of cell size, shape, and internal structure. While on flat, homogeneous and isotropic substrates some cells spread isotropically, others spread anisotropically and assume elongated structures. In addition, in their native environment as well as in vitro experiments, the cell shape and spreading asymmetry can be modulated by the local distribution of adhesive molecules and topography of the environment. We present a simple elastic model and experiments on stem cells to explain the variation of cell size with the matrix rigidity. In addition, we predict the experimental consequences of two mechanisms of acto-myosin polarization and focus here on the effect of the cell spreading asymmetry on the regulation of the stress-fiber alignment in the cytoskeleton. We show that when cell spreading is sufficiently asymmetric the alignment of acto-myosin forces in the cell increases monotonically with the matrix rigidity; however, in general this alignment is non-monotonic, as shown previously. These results highlight the importance of the symmetry characteristics of cell spreading in the regulation of cytoskeleton structure and suggest a mechanism by which different cell types may acquire different morphologies and internal structures in different mechanical environments."],["dc.identifier.doi","10.1088/0953-8984/22/19/194110"],["dc.identifier.isi","000277033100013"],["dc.identifier.pmid","20458358"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19900"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","0953-8984"],["dc.title","Cell shape, spreading symmetry, and the polarization of stress-fibers in cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Potyrala, Milena"],["dc.contributor.author","Brown, Andre E. X."],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:28:58Z"],["dc.date.available","2018-11-07T09:28:58Z"],["dc.date.issued","2013"],["dc.format.extent","151A"],["dc.identifier.isi","000316074301272"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30911"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Philadelphia, PA"],["dc.relation.issn","0006-3495"],["dc.title","Stem Cell Nucleus Morphology is Modulated by Matrix Mechanics via the Cytoskeleton"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","468"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nature Physics"],["dc.bibliographiccitation.lastpage","473"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Brown, A. E. X."],["dc.contributor.author","Discher, Dennis E."],["dc.contributor.author","Safran, S. A."],["dc.date.accessioned","2018-11-07T08:42:57Z"],["dc.date.available","2018-11-07T08:42:57Z"],["dc.date.issued","2010"],["dc.description.abstract","The shape and differentiated state of many cell types are highly sensitive to the rigidity of the microenvironment. The physical mechanisms involved, however, are unknown. Here, we present a theoretical model and experiments demonstrating that the alignment of stress fibres within stem cells is a non-monotonic function of matrix rigidity. We treat the cell as an active elastic inclusion in a surrounding matrix, allowing the actomyosin forces to polarize in response to elastic stresses developed in the cell. The theory correctly predicts the monotonic increase of the cellular forces with the matrix rigidity and the alignment of stress fibres parallel to the long axis of cells. We show that the anisotropy of this alignment depends non-monotonically on matrix rigidity and demonstrate it experimentally by quantifying the orientational distribution of stress fibres in stem cells. These findings offer physical insight into the sensitivity of stem-cell differentiation to tissue elasticity and, more generally, introduce a cell-type-specific parameter for actomyosin polarizability."],["dc.identifier.doi","10.1038/NPHYS1613"],["dc.identifier.isi","000279014400021"],["dc.identifier.pmid","20563235"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19826"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1745-2473"],["dc.title","Optimal matrix rigidity for stress-fibre polarization in stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Buxboim, A."],["dc.contributor.author","Swift, J."],["dc.contributor.author","Spinler, K. R."],["dc.contributor.author","Dingal, P. P."],["dc.contributor.author","Athirasala, A."],["dc.contributor.author","Kao, Y-R. C."],["dc.contributor.author","Harada, T."],["dc.contributor.author","Irianto, J."],["dc.contributor.author","Shin, J-W."],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:31:00Z"],["dc.date.available","2018-11-07T09:31:00Z"],["dc.date.issued","2013"],["dc.identifier.isi","000209348704223"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31442"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.title","How deeply cells feel: Matrix mechanics regulates lamin-A phosphorylation with feedback to actomyosin levels"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]