Rehfeldt, Florian

[["dc.bibliographiccitation.artnumber","e0189970"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PlOS ONE"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Franz, Jonas"],["dc.contributor.author","Grünebaum, Jonas"],["dc.contributor.author","Schäfer, Marcus"],["dc.contributor.author","Mulac, Dennis"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Langer, Klaus"],["dc.contributor.author","Kramer, Armin"],["dc.contributor.author","Riethmüller, Christoph"],["dc.date.accessioned","2019-07-09T11:45:07Z"],["dc.date.available","2019-07-09T11:45:07Z"],["dc.date.issued","2018"],["dc.description.abstract","Symmetry is rarely found on cellular surfaces. An exception is the brush border of microvilli, which are essential for the proper function of transport epithelia. In a healthy intestine, they appear densely packed as a 2D-hexagonal lattice. For in vitro testing of intestinal transport the cell line Caco-2 has been established. As reported by electron microscopy, their microvilli arrange primarily in clusters developing secondly into a 2D-hexagonal lattice. Here, atomic force microscopy (AFM) was employed under aqueous buffer conditions on Caco-2 cells, which were cultivated on permeable filter membranes for optimum differentiation. For analysis, the exact position of each microvillus was detected by computer vision; subsequent Fourier transformation yielded the type of 2D-lattice. It was confirmed, that Caco-2 cells can build a hexagonal lattice of microvilli and form clusters. Moreover, a second type of arrangement was discovered, namely a rhombic lattice, which appeared at sub-maximal densities of microvilli with (29 ± 4) microvilli / μm2. Altogether, the findings indicate the existence of a yet undescribed pattern in cellular organization."],["dc.description.sponsorship","Open-Access-Publikaionsfonds 2018"],["dc.identifier.doi","10.1371/journal.pone.0189970"],["dc.identifier.pmid","29320535"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15035"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59161"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Cell Culture Techniques"],["dc.subject.mesh","Cell Line, Tumor"],["dc.subject.mesh","Colonic Neoplasms"],["dc.subject.mesh","Enterocytes"],["dc.subject.mesh","Fourier Analysis"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Microscopy, Electron, Scanning"],["dc.subject.mesh","Microvilli"],["dc.title","Rhombic organization of microvilli domains found in a cell model of the human intestine"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]