Rehfeldt, Florian

[["dc.bibliographiccitation.artnumber","e0161623"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Prabhune, Meenakshi"],["dc.contributor.author","von Roden, Kerstin"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:44:43Z"],["dc.date.available","2017-09-07T11:44:43Z"],["dc.date.issued","2016"],["dc.description.abstract","Microtubule structure and functions have been widely studied in vitro and in cells. Research has shown that cysteines on tubulin play a crucial role in the polymerization of microtubules. Here, we show that blocking sulfhydryl groups of cysteines in taxol-stabilized polymerized microtubules with a commonly used chemical crosslinker prevents temporal end-to-end annealing of microtubules in vitro. This can dramatically affect the length distribution of the microtubules. The crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Interestingly, addition of a maleimide dye alone does not show the same interference with annealing in stabilized microtubules. This study shows that the sulfhydryl groups of cysteines of tubulin that are vital for the polymerization are also important for the subsequent annealing of microtubules."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0161623"],["dc.identifier.gro","3141632"],["dc.identifier.isi","000382258600066"],["dc.identifier.pmid","27561096"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3345"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.title","Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","51"],["dc.bibliographiccitation.journal","Progress in Biophysics and Molecular Biology"],["dc.bibliographiccitation.lastpage","60"],["dc.bibliographiccitation.volume","144"],["dc.contributor.author","Schlick, Susanne F."],["dc.contributor.author","Spreckelsen, Florian"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Iyer, Lavanya M."],["dc.contributor.author","Meyer, Tim"],["dc.contributor.author","Zelarayan, Laura C."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2020-12-10T15:20:42Z"],["dc.date.available","2020-12-10T15:20:42Z"],["dc.date.issued","2019"],["dc.description.abstract","Cardiomyocyte and stroma cell cross-talk is essential for the formation of collagen-based engineered heart muscle, including engineered human myocardium (EHM). Fibroblasts are a main component of the myocardial stroma. We hypothesize that fibroblasts, by compacting the surrounding collagen network, support the self-organization of cardiomyocytes into a functional syncytium. With a focus on early self-organization processes in EHM, we studied the molecular and biophysical adaptations mediated by defined populations of fibroblasts and embryonic stem cell-derived cardiomyocytes in a collagen type I hydrogel. After a short phase of cell-independent collagen gelation (30 min), tissue compaction was progressively mediated by fibroblasts. Fibroblast-mediated tissue stiffening was attenuated in the presence of cardiomyocytes allowing for the assembly of stably contracting, force-generating EHM within 4 weeks. Comparative RNA-sequencing data corroborated that fibroblasts are particularly sensitive to the tissue compaction process, resulting in the fast activation of transcription profiles, supporting heart muscle development and extracellular matrix synthesis. Large amplitude oscillatory shear (LAOS) measurements revealed nonlinear strain stiffening at physiological strain amplitudes (>2%), which was reduced in the presence of cells. The nonlinear stress-strain response could be characterized by a mathematical model. Collectively, our study defines the interplay between fibroblasts and cardiomyocytes during human heart muscle self-organization in vitro and underscores the relevance of fibroblasts in the biological engineering of a cardiomyogenesis-supporting viscoelastic stroma. We anticipate that the established mathematical model will facilitate future attempts to optimize EHM for in vitro (disease modelling) and in vivo applications (heart repair)."],["dc.identifier.doi","10.1016/j.pbiomolbio.2018.11.011"],["dc.identifier.pmid","30553553"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72769"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/248"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation","SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zelarayán-Behrend (Developmental Pharmacology)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.rights","CC BY 4.0"],["dc.title","Agonistic and antagonistic roles of fibroblasts and cardiomyocytes on viscoelastic stiffening of engineered human myocardium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","463001"],["dc.bibliographiccitation.issue","46"],["dc.bibliographiccitation.journal","Journal of Physics D: Applied Physics"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Schmidt, Christoph F."],["dc.date.accessioned","2019-07-09T11:44:30Z"],["dc.date.available","2019-07-09T11:44:30Z"],["dc.date.issued","2017"],["dc.description.abstract","In the last two decades, it has become evident that the mechanical properties of the microenvironment of biological cells are as important as traditional biochemical cues for the control of cellular behavior and fate. The field of cell and matrix mechanics is quickly growing and so is the development of the experimental approaches used to study active and passive mechanical properties of cells and their surroundings. Within this topical review we will provide a brief overview, on the one hand, over how cellular mechanics can be probed physically, how different geometries allow access to different cellular properties, and, on the other hand, how forces are generated in cells and transmitted to the extracellular environment. We will describe the following experimental techniques: atomic force microscopy, traction force microscopy, magnetic tweezers, optical stretcher and optical tweezers pointing out both their advantages and limitations. Finally, we give an outlook on the future of the physical probing of cells."],["dc.identifier.doi","10.1088/1361-6463/aa8aa6"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14805"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59027"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/340528/EU//CELLMECHANOCONTROL"],["dc.relation.issn","1361-6463"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Physical probing of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","397"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Investigative Ophthalmology & Visual Science"],["dc.bibliographiccitation.lastpage","406"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Gottschalk, Hanna M."],["dc.contributor.author","Wecker, Thomas"],["dc.contributor.author","Khattab, Mohammed H."],["dc.contributor.author","Fischer, Charlotte V."],["dc.contributor.author","Callizo, Josep"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Lubjuhn, Roswitha"],["dc.contributor.author","Russmann, Christoph"],["dc.contributor.author","Hoerauf, Hans"],["dc.contributor.author","van Oterendorp, Christian"],["dc.date.accessioned","2019-07-09T11:50:10Z"],["dc.date.available","2019-07-09T11:50:10Z"],["dc.date.issued","2019"],["dc.description.abstract","Purpose: Contrast agents applicable for optical coherence tomography (OCT) imaging are rare. The intrascleral aqueous drainage system would be a potential application for a contrast agent, because the aqueous veins are of small diameter and located deep inside the highly scattering sclera. We tested lipid emulsions (LEs) as candidate OCT contrast agents in vitro and ex vivo, including milk and the anesthetic substance Propofol. Methods: Commercial OCT and OCT angiography (OCTA) devices were used. Maximum reflectivity and signal transmission of LE were determined in tube phantoms. Absorption spectra and light scattering was analyzed. The anterior chamber of enucleated porcine eyes was perfused with LEs, and OCTA imaging of the LEs drained via the aqueous outflow tract was performed. Results: All LEs showed a significantly higher reflectivity than water (P < 0.001). Higher milk lipid content was positively correlated with maximum reflectivity and negatively with signal transmission. Propofol exhibited the best overall performance. Due to a high degree of signal fluctuation, OCTA could be applied for detection of LE. Compared with blood, the OCTA signal of Propofol was significantly stronger (P = 0.001). As a proof of concept, time-resolved aqueous angiography of porcine eyes was performed. The three-dimensional (3D) structure and dynamics of the aqueous outflow were significantly different from humans. Conclusions: LEs induced a strong signal in OCT and OCTA. LE-based OCTA allowed the ability to obtain time-resolved 3D datasets of aqueous outflow. Possible interactions of LE with inner eye's structures need to be further investigated before in vivo application."],["dc.identifier.doi","10.1167/iovs.18-25223"],["dc.identifier.pmid","30682210"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15874"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59715"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1552-5783"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Lipid Emulsion-Based OCT Angiography for Ex Vivo Imaging of the Aqueous Outflow Tract."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","551"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kaliman, Sara"],["dc.contributor.author","Jayachandran, Christina"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Smith, Ana-Sunčana"],["dc.date.accessioned","2019-07-09T11:42:54Z"],["dc.date.available","2019-07-09T11:42:54Z"],["dc.date.issued","2016"],["dc.description.abstract","It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description of such cell tiling. A particularly useful approach is the so called Voronoi tessellation, built from centers of mass of the cell nuclei (CMVT), which is commonly used for estimating the morphology of cells in epithelial tissues. However, a study providing a statistically sound analysis of this method's accuracy is not available in the literature. We addressed this issue here by comparing a number of morphological measures of the cells, including area, perimeter, and elongation obtained from such a tessellation with identical measures extracted from direct imaging acquired by staining the cell membranes. After analyzing the shapes of 15,000 MDCK II epithelial cells under several conditions, we find that CMVT reasonably well reproduces many of the morphological properties of the tissue with an error that is between 10 and 15%. Moreover, cross-correlations between different morphological measures are reproduced qualitatively correctly by this method. However, all of the properties including the cell perimeters, number of neighbors, and anisotropy measures often suffer from systematic or size dependent errors. These discrepancies originate from the polygonal nature of the tessellation which sets the limits of the applicability of CMVT."],["dc.identifier.doi","10.3389/fphys.2016.00551"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13989"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58782"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/337283/EU/Biological Membranes in Action: A Unified Approach to Complexation, Scaffolding and Active Transport/MEMBRANESACT"],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Limits of Applicability of the Voronoi Tessellation Determined by Centers of Cell Nuclei to Epithelium Morphology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","25"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Image Analysis & Stereology"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Benes, Viktor"],["dc.contributor.author","Vecera, Jakub"],["dc.contributor.author","Eltzner, Benjamin"],["dc.contributor.author","Wollnik, Carina"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Kralova, Veronika"],["dc.contributor.author","Huckemann, Stephan"],["dc.date.accessioned","2017-09-07T11:50:29Z"],["dc.date.available","2017-09-07T11:50:29Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.5566/ias.1627"],["dc.identifier.gro","3147619"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14778"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5090"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","chake"],["dc.publisher","Slovenian Society for Stereology and Quantitative Image Analysis"],["dc.relation.issn","1854-5165"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0/"],["dc.title","ESTIMATION OF PARAMETERS IN A PLANAR SEGMENT PROCESS WITH A BIOLOGICAL APPLICATION"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","443001"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Journal of Physics. D, Applied Physics"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Ando, Toshio"],["dc.contributor.author","Bhamidimarri, Satya Prathyusha"],["dc.contributor.author","Brending, Niklas"],["dc.contributor.author","Colin-York, H."],["dc.contributor.author","Collinson, Lucy"],["dc.contributor.author","De Jonge, Niels"],["dc.contributor.author","de Pablo, P. J."],["dc.contributor.author","Debroye, Elke"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Franck, Christian"],["dc.contributor.author","Fritzsche, Marco"],["dc.contributor.author","Gerritsen, Hans"],["dc.contributor.author","Giepmans, Ben N. G."],["dc.contributor.author","Grunewald, Kay"],["dc.contributor.author","Hofkens, Johan"],["dc.contributor.author","Hoogenboom, Jacob P."],["dc.contributor.author","Janssen, Kris P. F."],["dc.contributor.author","Kaufman, Rainer"],["dc.contributor.author","Klumpermann, Judith"],["dc.contributor.author","Kurniawan, Nyoman"],["dc.contributor.author","Kusch, Jana"],["dc.contributor.author","Liv, Nalan"],["dc.contributor.author","Parekh, Viha"],["dc.contributor.author","Peckys, Diana B."],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Reutens, David C."],["dc.contributor.author","Roeffaers, Maarten B. J."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Schwarz, Ulrich S."],["dc.contributor.author","Verkade, Paul"],["dc.contributor.author","Vogel, Michael W."],["dc.contributor.author","Wagner, Richard"],["dc.contributor.author","Winterhalter, Mathias"],["dc.contributor.author","Yuan, Haifeng"],["dc.contributor.author","Zifarelli, Giovanni"],["dc.date.accessioned","2020-03-10T15:26:08Z"],["dc.date.available","2020-03-10T15:26:08Z"],["dc.date.issued","2018"],["dc.description.abstract","Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints."],["dc.identifier.doi","10.1088/1361-6463/aad055"],["dc.identifier.pmid","30799880"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63287"],["dc.language.iso","en"],["dc.relation.issn","0022-3727"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","other"],["dc.title","The 2018 correlative microscopy techniques roadmap"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","690"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:54:39Z"],["dc.date.available","2017-09-07T11:54:39Z"],["dc.date.issued","2016"],["dc.description.abstract","Adult human mesenchymal stem cells show structural rearrangements of their cytoskeletal network during mechanically induced differentiation toward various cell types. In particular, the alignment of acto-myosin fibers is cell fate-dependent and can serve as an early morphological marker of differentiation. Quantification of such nanostructures on a mesoscopic scale requires high-resolution imaging techniques. Here, we use small-angle x-ray scattering with a spot size in the micro- and submicrometer range as a high-resolution and label-free imaging technique to reveal structural details of stem cells and differentiated cell types. We include principal component analysis into an automated empirical analysis scheme that allows the local characterization of oriented structures. Results on freeze-dried samples lead to quantitative structural information for all cell lines tested: differentiated cells reveal pronounced structural orientation and a relatively intense overall diffraction signal, whereas naive human mesenchymal stem cells lack these features. Our data support the hypothesis of stem cells establishing ordered structures along their differentiation process."],["dc.identifier.doi","10.1016/j.bpj.2015.12.017"],["dc.identifier.gro","3141731"],["dc.identifier.isi","000369467800017"],["dc.identifier.pmid","26840732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/446"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0126346"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Eltzner, Benjamin"],["dc.contributor.author","Wollnik, Carina"],["dc.contributor.author","Gottschlich, Carsten"],["dc.contributor.author","Huckemann, Stephan"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:48:37Z"],["dc.date.available","2017-09-07T11:48:37Z"],["dc.date.issued","2015"],["dc.description.abstract","A reliable extraction of filament data from microscopic images is of high interest in the analysis of acto-myosin structures as early morphological markers in mechanically guided differentiation of human mesenchymal stem cells and the understanding of the underlying fiber arrangement processes. In this paper, we propose the filament sensor (FS), a fast and robust processing sequence which detects and records location, orientation, length, and width for each single filament of an image, and thus allows for the above described analysis. The extraction of these features has previously not been possible with existing methods. We evaluate the performance of the proposed FS in terms of accuracy and speed in comparison to three existing methods with respect to their limited output. Further, we provide a benchmark dataset of real cell images along with filaments manually marked by a human expert as well as simulated benchmark images. The FS clearly outperforms existing methods in terms of computational runtime and filament extraction accuracy. The implementation of the FS and the benchmark database are available as open source."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.pone.0126346"],["dc.identifier.gro","3146928"],["dc.identifier.pmid","25996921"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11812"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4710"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Filament Sensor for Near Real-Time Detection of Cytoskeletal Fiber Structures"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1900317"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Macromolecular Rapid Communications"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Huang, Heqin"],["dc.contributor.author","Wang, Xiaojie"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Zhang, Kai"],["dc.contributor.author","Yang, Yang"],["dc.date.accessioned","2020-05-18T08:17:56Z"],["dc.date.available","2020-05-18T08:17:56Z"],["dc.date.issued","2019"],["dc.description.abstract","Controlling water transportation within hydrogels makes hydrogels attractive for diverse applications, but it is still a very challenging task. Herein, a novel type of dually electrostatically crosslinked nanocomposite hydrogel showing thermoresponsive water absorption, distribution, and dehydration processes are developed. The nanocomposite hydrogels are stabilized via electrostatic interactions between negatively charged poly(acrylic acid) and positively charged layered double hydroxide (LDH) nanosheets as well as poly(3-acrylamidopropyltrimethylammonium chloride). Both LDH nanosheets as crosslinkers and the surrounding temperatures played pivotal roles in tuning the water transportation within these nanocomposite hydrogels. By changing the surrounding temperature from 60 to 4 °C, these hydrogels showed widely adjustable swelling times between 2 and 45 days, while the dehydration process lasted between 7 and 27 days. A swift temperature decrease, for example, from 60 to 25 °C, generated supersaturation within these nanocomposite hydrogels, which further retarded the water transportation and distribution in hydrogel networks. Benefiting from modified water transportation and rapidly alternating water uptake capability during temperature change, pre-loaded compounds can be used to track and visualize these processes within nanocomposite hydrogels. At the same time, the discharge of water and loaded compounds from the interior of hydrogels demonstrates a thermoresponsive sustained release process."],["dc.description.sponsorship","German Research Foundation http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship","Fonds der Chemischen Industrie"],["dc.description.sponsorship","China Scholarship Council http://dx.doi.org/10.13039/501100004543"],["dc.description.sponsorship","Niedersächsische Ministerium für Wissenschaft und Kultur http://dx.doi.org/10.13039/100011937"],["dc.identifier.doi","10.1002/marc.201900317"],["dc.identifier.pmid","31433104"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65485"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.relation.eissn","1521-3927"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Thermoresponsive Water Transportation in Dually Electrostatically Crosslinked Nanocomposite Hydrogels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]