Rehfeldt, Florian

[["dc.bibliographiccitation.artnumber","463001"],["dc.bibliographiccitation.issue","46"],["dc.bibliographiccitation.journal","Journal of Physics D: Applied Physics"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Schmidt, Christoph F."],["dc.date.accessioned","2019-07-09T11:44:30Z"],["dc.date.available","2019-07-09T11:44:30Z"],["dc.date.issued","2017"],["dc.description.abstract","In the last two decades, it has become evident that the mechanical properties of the microenvironment of biological cells are as important as traditional biochemical cues for the control of cellular behavior and fate. The field of cell and matrix mechanics is quickly growing and so is the development of the experimental approaches used to study active and passive mechanical properties of cells and their surroundings. Within this topical review we will provide a brief overview, on the one hand, over how cellular mechanics can be probed physically, how different geometries allow access to different cellular properties, and, on the other hand, how forces are generated in cells and transmitted to the extracellular environment. We will describe the following experimental techniques: atomic force microscopy, traction force microscopy, magnetic tweezers, optical stretcher and optical tweezers pointing out both their advantages and limitations. Finally, we give an outlook on the future of the physical probing of cells."],["dc.identifier.doi","10.1088/1361-6463/aa8aa6"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14805"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59027"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/340528/EU//CELLMECHANOCONTROL"],["dc.relation.issn","1361-6463"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Physical probing of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","551"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kaliman, Sara"],["dc.contributor.author","Jayachandran, Christina"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Smith, Ana-Sunčana"],["dc.date.accessioned","2019-07-09T11:42:54Z"],["dc.date.available","2019-07-09T11:42:54Z"],["dc.date.issued","2016"],["dc.description.abstract","It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description of such cell tiling. A particularly useful approach is the so called Voronoi tessellation, built from centers of mass of the cell nuclei (CMVT), which is commonly used for estimating the morphology of cells in epithelial tissues. However, a study providing a statistically sound analysis of this method's accuracy is not available in the literature. We addressed this issue here by comparing a number of morphological measures of the cells, including area, perimeter, and elongation obtained from such a tessellation with identical measures extracted from direct imaging acquired by staining the cell membranes. After analyzing the shapes of 15,000 MDCK II epithelial cells under several conditions, we find that CMVT reasonably well reproduces many of the morphological properties of the tissue with an error that is between 10 and 15%. Moreover, cross-correlations between different morphological measures are reproduced qualitatively correctly by this method. However, all of the properties including the cell perimeters, number of neighbors, and anisotropy measures often suffer from systematic or size dependent errors. These discrepancies originate from the polygonal nature of the tessellation which sets the limits of the applicability of CMVT."],["dc.identifier.doi","10.3389/fphys.2016.00551"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13989"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58782"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/337283/EU/Biological Membranes in Action: A Unified Approach to Complexation, Scaffolding and Active Transport/MEMBRANESACT"],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Limits of Applicability of the Voronoi Tessellation Determined by Centers of Cell Nuclei to Epithelium Morphology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0189970"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PlOS ONE"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Franz, Jonas"],["dc.contributor.author","Grünebaum, Jonas"],["dc.contributor.author","Schäfer, Marcus"],["dc.contributor.author","Mulac, Dennis"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Langer, Klaus"],["dc.contributor.author","Kramer, Armin"],["dc.contributor.author","Riethmüller, Christoph"],["dc.date.accessioned","2019-07-09T11:45:07Z"],["dc.date.available","2019-07-09T11:45:07Z"],["dc.date.issued","2018"],["dc.description.abstract","Symmetry is rarely found on cellular surfaces. An exception is the brush border of microvilli, which are essential for the proper function of transport epithelia. In a healthy intestine, they appear densely packed as a 2D-hexagonal lattice. For in vitro testing of intestinal transport the cell line Caco-2 has been established. As reported by electron microscopy, their microvilli arrange primarily in clusters developing secondly into a 2D-hexagonal lattice. Here, atomic force microscopy (AFM) was employed under aqueous buffer conditions on Caco-2 cells, which were cultivated on permeable filter membranes for optimum differentiation. For analysis, the exact position of each microvillus was detected by computer vision; subsequent Fourier transformation yielded the type of 2D-lattice. It was confirmed, that Caco-2 cells can build a hexagonal lattice of microvilli and form clusters. Moreover, a second type of arrangement was discovered, namely a rhombic lattice, which appeared at sub-maximal densities of microvilli with (29 ± 4) microvilli / μm2. Altogether, the findings indicate the existence of a yet undescribed pattern in cellular organization."],["dc.description.sponsorship","Open-Access-Publikaionsfonds 2018"],["dc.identifier.doi","10.1371/journal.pone.0189970"],["dc.identifier.pmid","29320535"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15035"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59161"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Cell Culture Techniques"],["dc.subject.mesh","Cell Line, Tumor"],["dc.subject.mesh","Colonic Neoplasms"],["dc.subject.mesh","Enterocytes"],["dc.subject.mesh","Fourier Analysis"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Microscopy, Electron, Scanning"],["dc.subject.mesh","Microvilli"],["dc.title","Rhombic organization of microvilli domains found in a cell model of the human intestine"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]