Rehfeldt, Florian

[["dc.bibliographiccitation.artnumber","e0189970"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PlOS ONE"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Franz, Jonas"],["dc.contributor.author","Grünebaum, Jonas"],["dc.contributor.author","Schäfer, Marcus"],["dc.contributor.author","Mulac, Dennis"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Langer, Klaus"],["dc.contributor.author","Kramer, Armin"],["dc.contributor.author","Riethmüller, Christoph"],["dc.date.accessioned","2019-07-09T11:45:07Z"],["dc.date.available","2019-07-09T11:45:07Z"],["dc.date.issued","2018"],["dc.description.abstract","Symmetry is rarely found on cellular surfaces. An exception is the brush border of microvilli, which are essential for the proper function of transport epithelia. In a healthy intestine, they appear densely packed as a 2D-hexagonal lattice. For in vitro testing of intestinal transport the cell line Caco-2 has been established. As reported by electron microscopy, their microvilli arrange primarily in clusters developing secondly into a 2D-hexagonal lattice. Here, atomic force microscopy (AFM) was employed under aqueous buffer conditions on Caco-2 cells, which were cultivated on permeable filter membranes for optimum differentiation. For analysis, the exact position of each microvillus was detected by computer vision; subsequent Fourier transformation yielded the type of 2D-lattice. It was confirmed, that Caco-2 cells can build a hexagonal lattice of microvilli and form clusters. Moreover, a second type of arrangement was discovered, namely a rhombic lattice, which appeared at sub-maximal densities of microvilli with (29 ± 4) microvilli / μm2. Altogether, the findings indicate the existence of a yet undescribed pattern in cellular organization."],["dc.description.sponsorship","Open-Access-Publikaionsfonds 2018"],["dc.identifier.doi","10.1371/journal.pone.0189970"],["dc.identifier.pmid","29320535"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15035"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59161"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Cell Culture Techniques"],["dc.subject.mesh","Cell Line, Tumor"],["dc.subject.mesh","Colonic Neoplasms"],["dc.subject.mesh","Enterocytes"],["dc.subject.mesh","Fourier Analysis"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Microscopy, Electron, Scanning"],["dc.subject.mesh","Microvilli"],["dc.title","Rhombic organization of microvilli domains found in a cell model of the human intestine"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","422"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Integrative Biology"],["dc.bibliographiccitation.lastpage","430"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Brown, Andre E. X."],["dc.contributor.author","Raab, Matthew"],["dc.contributor.author","Cai, Shenshen"],["dc.contributor.author","Zajac, Allison L."],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:15:30Z"],["dc.date.available","2018-11-07T09:15:30Z"],["dc.date.issued","2012"],["dc.description.abstract","Physical features of microenvironments such as matrix elasticity E can clearly influence cell morphology and cell phenotype, but many differences between model matrices raise questions as to whether a standard biological scale for E exists, especially in 3D as well as in 2D. An E-series of two distinct types of hydrogels are ligand-functionalized here with non-fibrous collagen and used to elucidate wide-ranging cell and cytoskeletal responses to E in both 2D and 3D matrix geometries. Cross-linked hyaluronic acid (HA) based matrices as well as standard polyacrylamide (PA) hydrogels show that, within hours of initial plating, the adhesion, asymmetric shape, and cytoskeletal order within mesenchymal stem cells generally depend on E nonmonotonically over a broad range of physiologically relevant E. In particular, with overlays of a second matrix the stiffer of the upper or lower matrix dominates key cell responses to 3D: the cell invariably takes an elongated shape that couples to E in driving cytoplasmic stress fiber assembly. In contrast, embedding cells in homogeneous HA matrices constrains cells to spherically symmetric shapes in which E drives the assembly of a predominantly cortical cytoskeleton. Non-muscle myosin II generates the forces required for key cell responses and is a target of a phospho-Tyrosine signaling pathway that likely regulates contractile assemblies and also depends nonmonotonically on E. The results can be understood in part from a theory for stress fiber polarization that couples to matrix elasticity as well as cell shape and accurately predicts cytoskeletal order in 2D and 3D, regardless of polymer system."],["dc.identifier.doi","10.1039/c2ib00150k"],["dc.identifier.fs","597311"],["dc.identifier.isi","000302017100009"],["dc.identifier.pmid","22344328"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9576"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27709"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1757-9694"],["dc.relation.orgunit","Fakultät für Physik"],["dc.subject.mesh","Acrylic Resins"],["dc.subject.mesh","Actins"],["dc.subject.mesh","Cell Adhesion"],["dc.subject.mesh","Cell Proliferation"],["dc.subject.mesh","Cell Shape"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Collagen Type I"],["dc.subject.mesh","Elastic Modulus"],["dc.subject.mesh","Elasticity"],["dc.subject.mesh","Extracellular Matrix"],["dc.subject.mesh","Gelatin"],["dc.subject.mesh","Heterocyclic Compounds with 4 or More Rings"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Hyaluronic Acid"],["dc.subject.mesh","Hydrogels"],["dc.subject.mesh","Mesenchymal Stromal Cells"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Microscopy, Fluorescence"],["dc.subject.mesh","Myosin Heavy Chains"],["dc.subject.mesh","Nonmuscle Myosin Type IIA"],["dc.subject.mesh","Phosphorylation"],["dc.subject.mesh","Phosphotyrosine"],["dc.subject.mesh","Stress Fibers"],["dc.subject.mesh","Vinculin"],["dc.title","Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]