Rehfeldt, Florian

[["dc.bibliographiccitation.artnumber","443001"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Journal of Physics. D, Applied Physics"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Ando, Toshio"],["dc.contributor.author","Bhamidimarri, Satya Prathyusha"],["dc.contributor.author","Brending, Niklas"],["dc.contributor.author","Colin-York, H."],["dc.contributor.author","Collinson, Lucy"],["dc.contributor.author","De Jonge, Niels"],["dc.contributor.author","de Pablo, P. J."],["dc.contributor.author","Debroye, Elke"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Franck, Christian"],["dc.contributor.author","Fritzsche, Marco"],["dc.contributor.author","Gerritsen, Hans"],["dc.contributor.author","Giepmans, Ben N. G."],["dc.contributor.author","Grunewald, Kay"],["dc.contributor.author","Hofkens, Johan"],["dc.contributor.author","Hoogenboom, Jacob P."],["dc.contributor.author","Janssen, Kris P. F."],["dc.contributor.author","Kaufman, Rainer"],["dc.contributor.author","Klumpermann, Judith"],["dc.contributor.author","Kurniawan, Nyoman"],["dc.contributor.author","Kusch, Jana"],["dc.contributor.author","Liv, Nalan"],["dc.contributor.author","Parekh, Viha"],["dc.contributor.author","Peckys, Diana B."],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Reutens, David C."],["dc.contributor.author","Roeffaers, Maarten B. J."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Schwarz, Ulrich S."],["dc.contributor.author","Verkade, Paul"],["dc.contributor.author","Vogel, Michael W."],["dc.contributor.author","Wagner, Richard"],["dc.contributor.author","Winterhalter, Mathias"],["dc.contributor.author","Yuan, Haifeng"],["dc.contributor.author","Zifarelli, Giovanni"],["dc.date.accessioned","2020-03-10T15:26:08Z"],["dc.date.available","2020-03-10T15:26:08Z"],["dc.date.issued","2018"],["dc.description.abstract","Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints."],["dc.identifier.doi","10.1088/1361-6463/aad055"],["dc.identifier.pmid","30799880"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63287"],["dc.language.iso","en"],["dc.relation.issn","0022-3727"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","other"],["dc.title","The 2018 correlative microscopy techniques roadmap"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","690"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:54:39Z"],["dc.date.available","2017-09-07T11:54:39Z"],["dc.date.issued","2016"],["dc.description.abstract","Adult human mesenchymal stem cells show structural rearrangements of their cytoskeletal network during mechanically induced differentiation toward various cell types. In particular, the alignment of acto-myosin fibers is cell fate-dependent and can serve as an early morphological marker of differentiation. Quantification of such nanostructures on a mesoscopic scale requires high-resolution imaging techniques. Here, we use small-angle x-ray scattering with a spot size in the micro- and submicrometer range as a high-resolution and label-free imaging technique to reveal structural details of stem cells and differentiated cell types. We include principal component analysis into an automated empirical analysis scheme that allows the local characterization of oriented structures. Results on freeze-dried samples lead to quantitative structural information for all cell lines tested: differentiated cells reveal pronounced structural orientation and a relatively intense overall diffraction signal, whereas naive human mesenchymal stem cells lack these features. Our data support the hypothesis of stem cells establishing ordered structures along their differentiation process."],["dc.identifier.doi","10.1016/j.bpj.2015.12.017"],["dc.identifier.gro","3141731"],["dc.identifier.isi","000369467800017"],["dc.identifier.pmid","26840732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/446"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]