Hörandl, Elvira

[["dc.bibliographiccitation.artnumber","278"],["dc.bibliographiccitation.journal","Frontiers in Plant Science"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Klatt, Simone"],["dc.contributor.author","Hadacek, Franz"],["dc.contributor.author","Brinkmann, Gina"],["dc.contributor.author","Eilerts, Marius"],["dc.contributor.author","Hojsgaard, Diego H."],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2018-11-07T10:16:55Z"],["dc.date.available","2018-11-07T10:16:55Z"],["dc.date.issued","2016"],["dc.description.abstract","Meiosis, the key step of sexual reproduction, persists in facultative apomictic plants functional to some extent. However, it still remains unclear how and why proportions of reproductive pathways vary under different environmental stress conditions. We hypothesized that oxidative stress mediates alterations of developmental pathways. In apomictic plants we expected that megasporogenesis, the stage directly after meiosis, would be more affected than later stages of seed development. To simulate moderate stress conditions we subjected clone-mates of facultative apomictic Ranunculus auricomus to 10 h photoperiods, reflecting natural conditions, and extended ones (16.5 h). Reproduction mode was screened directly after megasporogenesis (microscope) and at seed stage (flow cytometric seed screening). Targeted metabolite profiles were performed with HPLC-DAD to explore if and which metabolic reprogramming was caused by the extended photoperiod. Prolonged photoperiods resulted in increased frequencies of sexual vs. aposporous initials directly after meiosis, but did not affect frequencies of sexual vs. asexual seed formation. Changes in secondary metabolite profiles under extended photoperiods affected all classes of compounds, and c. 20% of these changes separated the two treatments. Unexpectedly, the renowned antioxidant phenylpropanoids and flavonoids added more to clone-mate variation than to treatment differentiation. Among others, chlorophyll degradation products, non-assigned phenolic compounds and more lipophilic metabolites also contributed to the dissimilarity of the metabolic profiles of plants that had been exposed to the two different photoperiods. The hypothesis of moderate light stress effects was supported by increased proportions of sexual megaspore development at the expense of aposporous initial formation. The lack of effects at the seed stage confirms the basic assumption that only meiosis and sporogenesis would be sensitive to light stress. The concomitant change of secondary metabolite profiles, as a systemic response at this early developmental stage, supports the notion that oxidative stress could have affected megasporogenesis by causing the observed metabolic reprogramming. Hypotheses of genotype-specific responses to prolonged photoperiods are rejected."],["dc.description.sponsorship","Open-Access Publikationsfonds 2016"],["dc.identifier.doi","10.3389/fpls.2016.00278"],["dc.identifier.isi","000371400600001"],["dc.identifier.pmid","27014302"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12965"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41132"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-462X"],["dc.relation.issn","1664-462X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Photoperiod Extension Enhances Sexual Megaspore Formation and Triggers Metabolic Reprogramming in Facultative Apomictic Ranunculus auricomus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14435"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Ecology and Evolution"],["dc.bibliographiccitation.lastpage","14450"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Rahmsdorf, Elisabeth"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-04-14T08:30:57Z"],["dc.date.available","2021-04-14T08:30:57Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The larger distribution area of asexuals compared with their sexual relatives in geographical parthenogenesis (GP) scenarios has been widely attributed to the advantages of uniparental reproduction and polyploidy. However, potential disadvantages of sexuals due to their breeding system have received little attention so far. Here, we study the breeding system of five narrowly distributed sexual lineages of Ranunculus notabilis s.l. (R. auricomus complex) and its effects on outcrossing, inbreeding, female fitness, and heterozygosity. We performed selfing and intra‐ and interlineage crossings by bagging 481 flowers (59 garden individuals) followed by germination experiments. We compared seed set and germination rates, and related them to genetic distance and genome‐wide heterozygosity (thousands of RADseq loci). Selfings (2.5%) unveiled a significantly lower seed set compared with intra‐ (69.0%) and interlineage crossings (69.5%). Seed set of intra‐ (65%) compared to interpopulation crossings (78%) was significantly lower. In contrast, all treatments showed comparable germination rates (32%–43%). Generalized linear regressions between seed set and genetic distance revealed positive relationships in general and between lineages, and a negative one within lineages. Seed set was the main decisive factor for female fitness. Germination rates were not related to genetic distance at any level, but were positively associated with heterozygosity in interlineage crossings. Experiments confirmed full crossability and predominant outcrossing among sexual R. notabilis s.l. lineages. However, up to 5% (outliers 15%–31%) of seeds were formed by selfing, probably due to semi‐self‐compatibility in a multi‐locus gametophytic SI system. Less seed set in intrapopulation crossings, and higher seed set and germination rates from crossings of genetically more distant and heterozygous lineages (interlineage) indicate negative inbreeding and positive outbreeding effects. In GP scenarios, sexual species with small and/or isolated populations can suffer from decreased female fitness due to their breeding system. This factor, among others, probably limits range expansion of sexuals."],["dc.description.abstract","Potential disadvantages of sexual plant species due to their breeding system have received little attention in geographical parthenogenesis (GP) scenarios so far. We compared seed set and germination rates of five sexual lineages of Ranunculus notabilis s.l (R. auricomus complex) and related them to genetic distance and genome‐wide heterozygosity. Results suggest that inbreeding depression particularly affects intrapopulation (intralineage) crossings, and that positive outbreeding effects particularly influence interlineage crossings. In GP scenarios, sexual species with small and/or isolated populations can suffer from decreased female fitness due to their breeding system. image"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1002/ece3.7073"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17784"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83429"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/17835 but duplicate"],["dc.relation.eissn","2045-7758"],["dc.relation.issn","2045-7758"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Breeding system of diploid sexuals within the Ranunculus auricomus complex and its role in a geographical parthenogenesis scenario"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2031"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Ecology"],["dc.bibliographiccitation.lastpage","2049"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Paetzold, Claudia"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-04-14T08:25:24Z"],["dc.date.available","2021-04-14T08:25:24Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The time frame and geographical patterns of diversification processes in European temperate‐montane herbs are still not well understood. We used the sexual species of the Ranunculus auricomus complex as a model system to understand how vicariance versus dispersal processes in the context of Pleistocene climatic fluctuations have triggered speciation in temperate‐montane plant species. We used target enrichment sequence data from about 600 nuclear genes and coalescent‐based species tree inference methods to resolve phylogenetic relationships among the sexual taxa of the complex. We estimated absolute divergence times and, using ancestral range reconstruction, we tested if speciation was enhanced by vicariance or by dispersal processes. Phylogenetic relationships among taxa were fully resolved with some incongruence in the position of the tetraploid R. marsicus. Speciation events took place in a very short time at the end of the Mid‐Pleistocene Transition (830–580 thousand years ago [ka]). A second wave of intraspecific geographical differentiation occurred at the end of the Riss glaciation or during the Eemian interglacial between 200 and 100 ka. Ancestral range reconstruction suggests a widespread European ancestor of the R. auricomus complex. Vicariance has triggered allopatric speciation in temperate‐montane plant species during the climatic deterioration that occurred during the last phase of the Mid‐Pleistocene Transition. Vegetation restructuring from forest into tundra could have confined these forest species into isolated glacial macro‐ and microrefugia. During subsequent warming periods, range expansions of these species could have been hampered by apomictic derivatives and by other congeneric competitors in the same habitat."],["dc.description.sponsorship","German Research Foundation http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/mec.15458"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81617"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1365-294X"],["dc.relation.issn","0962-1083"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Phylogenomics unravels Quaternary vicariance and allopatric speciation patterns in temperate‐montane plant species: A case study on the Ranunculus auricomus species complex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Evolutionary Biology"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Klatt, Simone"],["dc.contributor.author","Hojsgaard, Diego"],["dc.contributor.author","Sharbel, Timothy F."],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2020-12-10T18:38:51Z"],["dc.date.available","2020-12-10T18:38:51Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1186/s12862-019-1495-z"],["dc.identifier.eissn","1471-2148"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16351"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77453"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A little bit of sex prevents mutation accumulation even in apomictic polyploid plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2659"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Ecology"],["dc.bibliographiccitation.lastpage","2675"],["dc.bibliographiccitation.volume","30"],["dc.contributor.affiliation","Tomasello, Salvatore; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Hodač, Ladislav; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Lorberg, Ellen; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Daubert, Mareike; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Lorberg, Ellen"],["dc.contributor.author","Daubert, Mareike"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-06-01T09:42:03Z"],["dc.date.available","2021-06-01T09:42:03Z"],["dc.date.issued","2021"],["dc.date.updated","2022-03-21T06:14:46Z"],["dc.description.abstract","Abstract Geographical parthenogenesis (GP) describes the phenomenon that apomicts tend to have larger distribution areas and/or occur at higher altitudes or latitudes compared to sexual relatives. However, the complex effects of genome‐wide heterozygosity, ploidy, reproduction mode (sexual vs. apomictic), and environment shaping GP of plants are still not well understood. We ascertained ploidy and reproduction mode by flow cytometry of 221 populations, and added genomic RADseq data (maximum 33,165 loci) of 80 taxa of the Ranunculus auricomus polyploid plant complex in temperate Europe. We observed 7% mainly diploid sexual, 28% facultative apomictic (mean sexuality 7.1%), and 65% obligate apomictic populations. Sexuals occupied a more southern, smaller distribution area, whereas apomicts expanded their range to higher latitudes. Within the complex, we detected three main genetic clusters and highly reticulate relationships. A genetically‐informed path analysis using GLMMs revealed several significant relationships. Sexuality of populations (percent of sexual seeds) was higher in diploids compared to polyploids, associated with more petals, and similar between forests and open habitats. In contrast to other apomictic plant complexes, sexuality was mainly positively correlated to solar radiation and isothermality, which fits the southern distribution. We found up to three times higher heterozygosity in polyploids compared to diploids, and generally more heterozygous individuals in forests compared with open habitats. Interestingly, we revealed a previously unknown positive association between heterozygosity and temperature seasonality, suggesting a higher resistance of polyploids to more extreme climatic conditions. We provide empirical evidence for intrinsic and extrinsic factors shaping the GP pattern in a polyploid plant complex."],["dc.description.sponsorship","German Research Foundation http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/mec.15919"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85129"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1365-294X"],["dc.relation.issn","0962-1083"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Moving beyond assumptions: Polyploidy and environmental effects explain a geographical parthenogenesis scenario in European plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]