Beham, Alexander Wilhelm

[["dc.bibliographiccitation.firstpage","418"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Immunobiology"],["dc.bibliographiccitation.lastpage","426"],["dc.bibliographiccitation.volume","218"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Kzhyshkowska, Julia"],["dc.contributor.author","Gratchev, Alexei"],["dc.contributor.author","Puellmann, Kerstin"],["dc.date.accessioned","2018-11-07T09:27:29Z"],["dc.date.available","2018-11-07T09:27:29Z"],["dc.date.issued","2013"],["dc.description.abstract","Since decades there is consensus among immunologists that in jawless and jawed vertebrates flexible immune recognition is strictly confined to the lymphoid lineage. In jawed vertebrates the adaptive immune system is represented by two lineages of lymphocytes. B cells and T cells that express recombinatorial antigen receptors of enormous diversity known as immunoglobulins and the T cell receptor (TCR). The recent identification of recombined immune receptors that are structurally based on the TCR in subpopulations of neutrophils and eosinophils (referred to here as TCR-like immunoreceptors, \"TCRL\") provides unexpected evidence for the existence of flexible host defense mechanisms beyond the realm of lymphocytes. Consistent with this, subpopulations of monocytes and macrophages from humans and mice now have also been shown to constitutively express recombined TCR-like immunoreceptors. Available in vitro evidence suggests that the TCRL in macrophages may exert functions as facilitators of phagocytosis and self-recruitment. More importantly, our recent findings that the macrophage-TCRL is implicated in granuloma formation in tuberculosis and the neutrophil-TCRL is associated with autoimmune hemolytic anemia establish for the first time a link between myeloid recombinatorial immune receptors and clinical disease. The discovery of recombined TCR-like immune receptors in granulocytes and macrophages extends the principle of combinatorial immune recognition to phagocytic cells. Conceptually, this unifies the two hitherto disparate cardinal features of innate and adaptive immunity, phagocytic capacity and recombinatorial immune recognition on a common cellular platform. Moreover, it strongly suggests that flexible host defense in vertebrates may operate on a broader cellular basis than currently thought. (C) 2012 Published by Elsevier GmbH."],["dc.identifier.doi","10.1016/j.imbio.2012.05.024"],["dc.identifier.isi","000316235700015"],["dc.identifier.pmid","22749215"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30547"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","0171-2985"],["dc.title","On the horizon: Flexible immune recognition outside lymphocytes"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","The Journal of Immunology"],["dc.bibliographiccitation.volume","182"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Kzhyshkowska, Julia"],["dc.contributor.author","Gratchev, Alexei"],["dc.contributor.author","Laird, Rebecca"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Ganser, Arnold"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T08:31:11Z"],["dc.date.available","2018-11-07T08:31:11Z"],["dc.date.issued","2009"],["dc.identifier.isi","000209763602309"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17065"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Immunologists"],["dc.publisher.place","Bethesda"],["dc.relation.issn","1550-6606"],["dc.relation.issn","0022-1767"],["dc.title","Macrophages express a TCR alpha beta-based variable immunoreceptor"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","39"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Immunobiology"],["dc.bibliographiccitation.lastpage","44"],["dc.bibliographiccitation.volume","222"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Hahn, Martin"],["dc.contributor.author","Riabov, Vladimir"],["dc.contributor.author","Yin, Shuiping"],["dc.contributor.author","Kzhyshkowska, Julia"],["dc.contributor.author","Busch, Svenja"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T10:29:34Z"],["dc.date.available","2018-11-07T10:29:34Z"],["dc.date.issued","2017"],["dc.description.abstract","Recent evidence indicates the presence of macrophage subpopulations that express the TCR alpha beta in two major inflammatory diseases, tuberculosis and atherosclerosis. Inflammation is also a well-established attribute of cancer progression and macrophages are one of the major immune cells that infiltrate tumors. Here, we demonstrate that the macrophage-TCR alpha beta is expressed in the tumor microenvironment of human and murine malignancies. We identify TCR alpha beta(+) macrophages in each case of four randomly selected distinct human tumor entities. In human tumor tissues, the TCR alpha beta expressed by macrophages in the tumor microenvironment is a combinatorial and individual-specific immune receptor. Furthermore, we routinely find TCR alpha beta(+) macrophage subpopulations in experimental tumors (TS/A, mammary adenocarcinoma) which we induced both in normal mice and mice deficient in the macrophage receptor stabilin-1. Expression of the combinatorial murine tumor macrophage TCRotS is individual-specific and independent of stabilin-1. These results demonstrate that TCR alpha beta expression is a characteristic feature of macrophages in the tumor microenvironment and identify an as yet unrecognized flexible element in the macrophage-based host response to tumors. (C) 2015 Elsevier GmbH. All rights reserved."],["dc.identifier.doi","10.1016/j.imbio.2015.09.022"],["dc.identifier.isi","000390613800005"],["dc.identifier.pmid","26494401"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43664"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","0171-2985"],["dc.title","A combinatorial alpha beta T cell receptor expressed by macrophages in the tumor microenvironment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Neurology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Dreyfus, David H."],["dc.contributor.author","Piehler, Armin P."],["dc.contributor.author","Reuter, Björn"],["dc.contributor.author","Schwarzbach, Christopher"],["dc.contributor.author","Willmann, Olaf"],["dc.contributor.author","Yepes, Diego"],["dc.contributor.author","Costina, Victor"],["dc.contributor.author","Findeisen, Peter"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Wang, Chunlin"],["dc.contributor.author","Han, Jian"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2020-12-10T18:44:32Z"],["dc.date.available","2020-12-10T18:44:32Z"],["dc.date.issued","2019"],["dc.description.abstract","Bacterial meningitis is a life-threatening disease that evokes an intense neutrophil-dominated host response to microbes invading the subarachnoid space. Recent evidence indicates the existence of combinatorial V(D)J immune receptors in neutrophils that are based on the T cell receptor (TCR). Here, we investigated expression of the novel neutrophil TCRαβ-based V(D)J receptors in cerebrospinal fluid (CSF) from human patients with acute-phase bacterial meningitis using immunocytochemical, genetic immunoprofiling, cell biological, and mass spectrometric techniques. We find that the human neutrophil combinatorial V(D)J receptors are rapidly induced in CSF neutrophils during the first hours of bacterial meningitis. Immune receptor repertoire diversity is consistently increased in CSF neutrophils relative to circulating neutrophils and phagocytosis of baits directed to the variable immunoreceptor is enhanced in CSF neutrophils during acute-phase meningitis. Our results reveal that a flexible immune response involving neutrophil V(D)J receptors which enhance phagocytosis is immediately initiated at the site of acute bacterial infection."],["dc.identifier.doi","10.3389/fneur.2019.00307"],["dc.identifier.eissn","1664-2295"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78494"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-2295"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Immediate Neutrophil-Variable-T Cell Receptor Host Response in Bacterial Meningitis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","404"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Aging and Disease"],["dc.bibliographiccitation.lastpage","413"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Puellmann, Kerstin"],["dc.date.accessioned","2018-11-07T09:05:34Z"],["dc.date.available","2018-11-07T09:05:34Z"],["dc.date.issued","2012"],["dc.description.abstract","Longstanding immunological dogma holds that flexible immune recognition, which forms the mechanistic basis of adaptive immunity, is strictly confined to the lymphocyte lineage. In higher vertebrates, flexible immune recognition is represented by recombinatorial antigen receptors of enormous diversity known as immunoglobulins, expressed by B lymphocytes, and the T cell receptor (TCR), expressed by T lymphocytes. The recent discovery of recombinatorial immune receptors that are structurally based on the TCR (referred to as TCR-like immunoreceptors, \"TCRL\") in myeloid phagocytes such as neutrophils and monocytes/macrophages now challenges the lymphocentric paradigm of flexible immunity. Here, we introduce the emerging concept of \"extralymphocytic flexible immune recognition\" and discuss its implications for inflammation and aging."],["dc.description.sponsorship","Deutsche Vereinte Gesellschaft fur Klinische Chemie und Labormedizin"],["dc.identifier.isi","000208951500005"],["dc.identifier.pmid","23185720"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Soc Aging & Disease"],["dc.relation.issn","2152-5250"],["dc.title","Extralymphocytic Flexible Immune Recognition: a New Angle on Inflammation and Aging"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9823"],["dc.bibliographiccitation.journal","The Lancet"],["dc.bibliographiccitation.volume","379"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Schneider, Sven"],["dc.contributor.author","Kruth, Jens"],["dc.contributor.author","Schulze, Torsten J."],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T09:11:14Z"],["dc.date.available","2018-11-07T09:11:14Z"],["dc.date.issued","2012"],["dc.format.extent","1364"],["dc.identifier.isi","000302531100035"],["dc.identifier.pmid","22483032"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26671"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0140-6736"],["dc.title","An autoimmune double attack"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","960"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Immunobiology"],["dc.bibliographiccitation.lastpage","968"],["dc.bibliographiccitation.volume","218"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Hahn, Martin"],["dc.contributor.author","Dollt, Claudia"],["dc.contributor.author","Pechlivanidou, Ioanna"],["dc.contributor.author","Ovsiy, Ilja"],["dc.contributor.author","Kzhyshkowska, Julia"],["dc.contributor.author","Gratchev, Alexei"],["dc.contributor.author","Fleig, Julian"],["dc.contributor.author","Emmert, Alexander"],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T09:23:25Z"],["dc.date.available","2018-11-07T09:23:25Z"],["dc.date.issued","2013"],["dc.description.abstract","Recent evidence indicates that monocytes and macrophages express T cell receptor (TCR)alpha beta-like combinatorial immune receptors. Here, we demonstrate the presence of a second recombinatorial immunoreceptor, which is structurally based on the TCR gamma- and delta-chains, in human and murine monocytes and differentially activated macrophages (referred to here as TCRLm gamma delta). In vitro, infection of macrophages with mycobacteria and gram positive or gram negative bacteria induced expression of donor-specific and differential TCRLm V delta repertoires indicating that the novel immunoreceptor represents a dynamic flexible host defense system that responds to bacterial challenge. In vivo, we find that TCRLm gamma delta bearing macrophages, which express highly restricted repertoires of the antigen-binding V delta chain, accumulate in the cerebrospinal fluid in acute bacterial meningitis and in advanced lesions of atherosclerosis. These results identify an as yet unrecognized monocyte/macrophage subpopulation that bears combinatorial TCRLm gamma delta immune receptors, and is associated with both acute and chronic inflammatory diseases. Moreover, they indicate that the monocytic lineage uses the same bipartite system of TCR alpha beta/TCR gamma delta-based combinatorial immune receptors that is present in T cells. Our findings suggest specific roles of monocytes/macrophages in various inflammatory conditions and lend further evidence that flexible immune recognition in higher vertebrates operates on a broader cellular basis than previously thought. (c) 2013 Published by Elsevier GmbH."],["dc.description.sponsorship","Stiftung Pathobiochemie (DGKL); DFG [GRK880/3]; BMBF [RUS 10/B05]"],["dc.identifier.doi","10.1016/j.imbio.2012.11.005"],["dc.identifier.isi","000320485300004"],["dc.identifier.pmid","23312956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29573"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","0171-2985"],["dc.title","A second combinatorial immune receptor in monocytes/macrophages is based on the TCR gamma delta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e0204108"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Hahn, Martin"],["dc.contributor.author","Ries, Lukas"],["dc.contributor.author","Giesler, Sophie"],["dc.contributor.author","Busch, Svenja"],["dc.contributor.author","Wang, Chunlin"],["dc.contributor.author","Han, Jian"],["dc.contributor.author","Schulze, Torsten J."],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.editor","Haziot, Alain"],["dc.date.accessioned","2020-12-10T18:42:09Z"],["dc.date.available","2020-12-10T18:42:09Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1371/journal.pone.0204108"],["dc.identifier.eissn","1932-6203"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77824"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Expression of combinatorial immunoglobulins in macrophages in the tumor microenvironment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Clinical Chemistry and Laboratory Medicine (CCLM)"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Scharfenstein, Olivia"],["dc.contributor.author","Stobe, Elfi"],["dc.contributor.author","Eichner, Romy"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T08:56:24Z"],["dc.date.available","2018-11-07T08:56:24Z"],["dc.date.issued","2011"],["dc.format.extent","S113"],["dc.identifier.isi","000289974200123"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23144"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Walter De Gruyter & Co"],["dc.publisher.place","Berlin"],["dc.relation.issn","1434-6621"],["dc.title","CHARACTERIZATION OF THE NEUTROPHIL-TCR IMMUNE RECEPTOR IN PATIENTS WITH SEPSIS OR SIRS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1002375"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Puellmann, Kerstin"],["dc.contributor.author","Laird, Rebecca"],["dc.contributor.author","Fuchs, Tina"],["dc.contributor.author","Streich, Roswita"],["dc.contributor.author","Breysach, Caroline"],["dc.contributor.author","Raddatz, Dirk"],["dc.contributor.author","Oniga, Septimia"],["dc.contributor.author","Peccerella, Teresa"],["dc.contributor.author","Findeisen, Peter"],["dc.contributor.author","Kzhyshkowska, Julia"],["dc.contributor.author","Gratchev, Alexei"],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Saunders, Bernadette"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Keane, Joseph"],["dc.contributor.author","Becker, Heinz"],["dc.contributor.author","Ganser, Arnold"],["dc.contributor.author","Neumaier, Michael"],["dc.contributor.author","Kaminski, Wolfgang E."],["dc.date.accessioned","2018-11-07T08:50:08Z"],["dc.date.available","2018-11-07T08:50:08Z"],["dc.date.issued","2011"],["dc.description.abstract","Macrophages play a central role in host defense against mycobacterial infection and anti-TNF therapy is associated with granuloma disorganization and reactivation of tuberculosis in humans. Here, we provide evidence for the presence of a T cell receptor (TCR) alpha beta based recombinatorial immune receptor in subpopulations of human and mouse monocytes and macrophages. In vitro, we find that the macrophage-TCR alpha beta induces the release of CCL2 and modulates phagocytosis. TNF blockade suppresses macrophage-TCR alpha beta expression. Infection of macrophages from healthy individuals with mycobacteria triggers formation of clusters that express restricted TCR V beta repertoires. In vivo, TCR alpha beta bearing macrophages abundantly accumulate at the inner host-pathogen contact zone of caseous granulomas from patients with lung tuberculosis. In chimeric mouse models, deletion of the variable macrophage-TCR alpha beta or TNF is associated with structurally compromised granulomas of pulmonary tuberculosis even in the presence of intact T cells. These results uncover a TNF-regulated recombinatorial immune receptor in monocytes/macrophages and demonstrate its implication in granuloma formation in tuberculosis."],["dc.identifier.doi","10.1371/journal.ppat.1002375"],["dc.identifier.isi","000297337300037"],["dc.identifier.pmid","22114556"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7793"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21628"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7374"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","A TNF-Regulated Recombinatorial Macrophage Immune Receptor Implicated in Granuloma Formation in Tuberculosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]