Buhl, Timo

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","JDDG Journal der Deutschen Dermatologischen Gesellschaft"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Richter, A."],["dc.contributor.author","Haas, E."],["dc.contributor.author","Holzkamp, R."],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.date.accessioned","2018-11-07T08:52:36Z"],["dc.date.available","2018-11-07T08:52:36Z"],["dc.date.issued","2011"],["dc.format.extent","781"],["dc.identifier.isi","000294596900143"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22210"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.issn","1610-0379"],["dc.title","Radiotherapy in thyroid Eye Disease: Ocurrence of pigmented Basal Cell Carcinomas with 15 years Latency"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","625"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Der Hautarzt"],["dc.bibliographiccitation.lastpage","626"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.date.accessioned","2018-11-07T08:53:30Z"],["dc.date.available","2018-11-07T08:53:30Z"],["dc.date.issued","2011"],["dc.description.abstract","A 16-year-old boy with multiple nevi presented with an enlarging pigmented lesion on his right chest. Previously excised nevi were all benign. Dermoscopy of the symmetrical lesion revealed a trizonal globular-homogeneous pattern. A peripheral rim of brown globules was followed by a zone with homogeneous pigmentation. The centre of the lesion showed black dots and globules. Histopathology confirmed a dysplastic compound nevus and found no diagnostic aspects of pigmented spindle cell nevus (Reed)."],["dc.identifier.doi","10.1007/s00105-011-2191-0"],["dc.identifier.isi","000293539500014"],["dc.identifier.pmid","21681540"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22426"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0017-8470"],["dc.title","Symmetrically enlarging pigmented lesion in an adolescent"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","JDDG Journal der Deutschen Dermatologischen Gesellschaft"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Hoffmann, S."],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Rosenberger, Albert"],["dc.date.accessioned","2018-11-07T09:36:15Z"],["dc.date.available","2018-11-07T09:36:15Z"],["dc.date.issued","2014"],["dc.format.extent","15"],["dc.identifier.isi","000340532500037"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32571"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1610-0387"],["dc.relation.issn","1610-0379"],["dc.title","Determination of Patient Characteristics in Specific Melanoma Subtypes by means of a Prospective Observational Study"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","37"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","JDDG Journal der Deutschen Dermatologischen Gesellschaft"],["dc.bibliographiccitation.lastpage","45"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Hoffmann, Saskia"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Bertsch, Hans Peter"],["dc.contributor.author","Rosenberger, Albert"],["dc.date.accessioned","2018-11-07T10:04:00Z"],["dc.date.available","2018-11-07T10:04:00Z"],["dc.date.issued","2015"],["dc.description.abstract","BackgroundCertain melanoma histotypes carry a worse prognosis than others. We aimed to identify patient related factors associated with specific melanoma histotypes. Patients and methodsSingle center study including 347 melanoma patients, prospectively assessed for 22 variables leading to a database of more than 7600 features. ResultsMelanomas were histologically categorized as superficial spreading (SSM, 70.6%), nodular (NM; 12.7%), acrolentiginous (ALM; 4.0%), lentigo maligna (LMM; 3.8%), or unclassified melanoma (UCM; 8.9%). Well recognized melanoma risk indicators (i.e. many atypical nevi, freckles, previous melanoma), were significantly associated with SSM and LMM histotypes. NM and ALM patients carried significantly less common and/or atypical nevi. NM were mostly self-detected or detected by relatives. In contrast, SSM, LMM, and ALM were most frequently detected by dermatologists. NM and UCM were preferentially located on poorly observable sites, SSM on the lower limbs, ALM on plantar sites, and LMM on the head and neck. ALM and LMM patients were significantly older than other patients. A multinomial logistic model was designed to predict a certain melanoma histotype (overall accuracy 81%), which could be helpful to focus the attention of clinicians or may be integrated into fully automated diagnostic algorithms. ConclusionsMelanoma histotypes show significant differences regarding patients' characteristics."],["dc.identifier.doi","10.1111/ddg.12561"],["dc.identifier.isi","000347445900006"],["dc.identifier.pmid","25640492"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38598"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1610-0387"],["dc.relation.issn","1610-0379"],["dc.title","Assessment of melanoma histotypes and associated patient related factors: Basis for a predictive statistical model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","JDDG Journal der Deutschen Dermatologischen Gesellschaft"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Hansen-Hagge, Christian"],["dc.contributor.author","Korpas, Bianca"],["dc.contributor.author","Kaune, Kjell Matthias"],["dc.contributor.author","Rosenberger, Albert"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, M."],["dc.date.accessioned","2018-11-07T08:52:36Z"],["dc.date.available","2018-11-07T08:52:36Z"],["dc.date.issued","2011"],["dc.format.extent","766"],["dc.identifier.isi","000294596900091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22209"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.issn","1610-0379"],["dc.title","Melanoma Early Detction with the Algorithm DynaMel: Combined dermatoscopic dynamic Evaluation and static Criteria"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Braun, Alexander"],["dc.contributor.author","Forkel, Susann"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","van Werven, Lars"],["dc.contributor.author","Jahn, Olaf"],["dc.contributor.author","Rezaei-Ghaleh, Nasrollah"],["dc.contributor.author","Zweckstetter, Markus"],["dc.contributor.author","Mempel, Martin"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.date.accessioned","2018-11-07T09:43:01Z"],["dc.date.available","2018-11-07T09:43:01Z"],["dc.date.issued","2014"],["dc.format.extent","E32"],["dc.identifier.isi","000332335500205"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34085"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.eventlocation","Cologne, GERMANY"],["dc.relation.issn","1600-0625"],["dc.relation.issn","0906-6705"],["dc.title","Routes of internalization for Melan-A/TAT fusion peptides differ considerably between human dendritic cells and other non-phagocytic cell types"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","337"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of the American Academy of Dermatology"],["dc.bibliographiccitation.lastpage","341"],["dc.bibliographiccitation.volume","61"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Kaune, Kjell Matthias"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Thoms, Kai-Martin"],["dc.contributor.author","Padeken, Michael"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, Michael Peter"],["dc.date.accessioned","2018-11-07T08:27:21Z"],["dc.date.available","2018-11-07T08:27:21Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: Nevus spilus (NS) defines a cafe-au-lait macule with superimposed maculopapular speckles. Although the cafe-au-lait macule often presents at birth, the darker pigmented speckles increase in number and size during a period of several years. The need for close follow-Lip of patients with NS is underlined by reports of several cases of cutaneous melanoma developing within Such lesions. Methods: We followed up 4 adult patients (3 male, one female; mean age 38 years) with Unilateral segmental NS of the thoracic or abdominal region. The NS was present at birth in all 4 patients. Follow-up by sequential digital dermatoscopy and digital overview images was scheduled every 6 to 12 months. Results: During surveillance (mean follow-up time, 8.1.5 years), 3 melanocytic lesions were excised. in one patient focal enlargement prompted excision of two dysplastic compound nevi. In another patient new black dots and focal peripheral hyperpign-lentation were detected by comparison with previous images. Histologic analysis confirmed the diagnosis of invasive melanoma (Breslow thickness, 0.6 mm). Limitations: This observational clinical study included a small number of patients. Sequential digital dermatoscopy of large NS in children may lead to Unnecessary excisions because of physiologic changes. Conclusion: We suggest close follow-up of patients with segmental NS whenever complete excision is not possible. In adults, follow-tip by digital dermatoscopy and excision of lesions with dynamic changes may assist in the early detection of melanoma. (J Am Acad Dermatol 2009;61:337-41.)"],["dc.identifier.doi","10.1016/j.jaad.2008.12.035"],["dc.identifier.isi","000268775500020"],["dc.identifier.pmid","19233509"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16191"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mosby-elsevier"],["dc.relation.issn","0190-9622"],["dc.title","Melanoma arising in segmental nevus spilus: Detection by sequential digital dermatoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","140"],["dc.bibliographiccitation.journal","JDDG Journal der Deutschen Dermatologischen Gesellschaft"],["dc.bibliographiccitation.lastpage","141"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Mograby, Nerjes"],["dc.contributor.author","Ngassa, A."],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Brehmer, Franziska"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Schoen, M."],["dc.contributor.author","Rosenberger, Albert"],["dc.contributor.author","Bertsch, Hans-Peter"],["dc.date.accessioned","2018-11-07T09:58:44Z"],["dc.date.available","2018-11-07T09:58:44Z"],["dc.date.issued","2015"],["dc.identifier.isi","000360219000395"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37429"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1610-0387"],["dc.relation.issn","1610-0379"],["dc.title","Primary Melanoma in Association with pre Navi: Results of a prospective Observational Study involving 832 High Risk Patients"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","785"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of the American Academy of Dermatology"],["dc.bibliographiccitation.lastpage","793"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.contributor.author","Korpas, Bianca"],["dc.contributor.author","Hansen-Hagge, Christian"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Kaune, Kjell Matthias"],["dc.contributor.author","Rosenberger, Albert"],["dc.contributor.author","Krueger, Ullrich"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Emmert, Steffen"],["dc.date.accessioned","2018-11-07T08:43:40Z"],["dc.date.available","2018-11-07T08:43:40Z"],["dc.date.issued","2010"],["dc.description.abstract","Background. The retrospectively developed 7-point checklist is one of the most applicable dermatoscopic algorithms for clinical use. However, until today no prospective data on the diagnostic performance of this algorithm were reported. Objective: Our aim was to assess the sensitivity, specificity, and diagnostic accuracy of the 7-point checklist in the setting of a prospective long-term study. Methods: Patients at increased melanoma risk (n = 688) were screened at regular intervals by naked-eye examination, the dermatoscopic 7-point checklist, and digital dermatoscopy follow-up (10-year study interval). Results: We detected 127 melanomas including 50 melanomas in situ. The mean Breslow thickness of invasive melanomas was 0.57 mm. A total of 79 melanomas displayed the 7-point checklist melanoma threshold of 3 or more points (62% sensitivity, compared with 78%-95% in retrospective settings). In all, 48 melanomas scored fewer than 3 points and were excised because of complementary information (eg, lesional history, dynamic changes detected by digital dermatoscopy). The specificity of the 7-point checklist was 97% (compared with 65%-87% in retrospective settings). Regression patterns, atypical vascular patterns, and radial streaming were associated with the highest relative risk for melanoma (odds ratio 3.26, 95% confidence interval 2.05-5.16; odds ratio 3.04, 95% confidence interval 1.70-5.46; odds ratio 2.91, 95% confidence interval 1.64-5.15; P < .0003, respectively). Melanomas thicker than 0.5 mm exhibited significantly more regression patterns and atypical vascular patterns (P < .02). The malignant versus benign ratio for all excised lesions was 1:8.6 (127 melanomas, 1092 nonmelanomas). Limitations: Calculation of the specificity was a limitation. True negative lesions were defined by a score less than 3 points and either the histopathological diagnosis of nonmelanoma or the absence of dynamic changes during digital dermatoscopy follow-up (nonexcised, nonsuspicious, no change). Conclusions: The 7-point checklist for dermatoscopy was less sensitive but highly specific in this prospective clinical setting. Complementary information clearly increased the sensitivity. Regression patterns or radial streaming in nevi of patients at high risk should raise a higher melanoma suspicion than might be concluded from retrospective studies. (J Am Acad Dermatol 2010;62:785-93.)"],["dc.identifier.doi","10.1016/j.jaad.2009.08.049"],["dc.identifier.isi","000277190100007"],["dc.identifier.pmid","20226567"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20023"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mosby-elsevier"],["dc.relation.issn","0190-9622"],["dc.title","Seven-point checklist for dermatoscopy: Performance during 10 years of prospective surveillance of patients at increased melanoma risk"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2021"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cancer Immunology Immunotherapy"],["dc.bibliographiccitation.lastpage","2031"],["dc.bibliographiccitation.volume","61"],["dc.contributor.author","Buhl, Timo"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Rosenberger, Albert"],["dc.contributor.author","Schardt, Anke"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Haenssle, Holger Andreas"],["dc.date.accessioned","2018-11-07T09:03:57Z"],["dc.date.available","2018-11-07T09:03:57Z"],["dc.date.issued","2012"],["dc.description.abstract","Availability of large quantities of functionally effective dendritic cells (DC) represents one of the major challenges for immunotherapeutic trials against infectious or malignant diseases. Low numbers or insufficient T-cell activation of DC may result in premature termination of treatment and unsatisfying immune responses in clinical trials. Based on the notion that cryopreservation of monocytes is superior to cryopreservation of immature or mature DC in terms of resulting DC quantity and immuno-stimulatory capacity, we aimed to establish an optimized protocol for the cryopreservation of highly concentrated peripheral blood mononuclear cells (PBMC) for DC-based immunotherapy. Cryopreserved cell preparations were analyzed regarding quantitative recovery, viability, phenotype, and functional properties. In contrast to standard isopropyl alcohol (IPA) freezing, PBMC cryopreservation in an automated controlled-rate freezer (CRF) with subsequent thawing and differentiation resulted in significantly higher cell yields of immature and mature DC. Immature DC yields and total protein content after using CRF were comparable with results obtained with freshly prepared PBMC and exceeded results of standard IPA freezing by approximately 50 %. While differentiation markers, allogeneic T-cell stimulation, viability, and cytokine profiles were similar to DC from standard freezing procedures, DC generated from CRF-cryopreserved PBMC induced a significantly higher antigen-specific IFN-gamma release from autologous effector T cells. In summary, automated controlled-rate freezing of highly concentrated PBMC represents an improved method for increasing DC yields and autologous T-cell stimulation."],["dc.description.sponsorship","University Medical Center Gottingen for young researchers"],["dc.identifier.doi","10.1007/s00262-012-1262-0"],["dc.identifier.isi","000310888400012"],["dc.identifier.pmid","22527251"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8798"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25006"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-7004"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Controlled-rate freezer cryopreservation of highly concentrated peripheral blood mononuclear cells results in higher cell yields and superior autologous T-cell stimulation for dendritic cell-based immunotherapy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]