Stark, Holger

[["dc.bibliographiccitation.firstpage","267"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","278"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Golas, Monika M."],["dc.contributor.author","Makarov, Evgeny M."],["dc.contributor.author","Brahms, Hero"],["dc.contributor.author","Kastner, Berthold"],["dc.contributor.author","Lührmann, Reinhard"],["dc.contributor.author","Stark, Holger"],["dc.date.accessioned","2021-03-05T08:58:09Z"],["dc.date.available","2021-03-05T08:58:09Z"],["dc.date.issued","2006"],["dc.identifier.doi","10.1016/j.molcel.2006.08.021"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80026"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1097-2765"],["dc.title","Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","766"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.lastpage","778"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Golas, Mariola Monika"],["dc.contributor.author","Boehm, Cordula"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Effenberger, Kerstin"],["dc.contributor.author","Brecht, Michael"],["dc.contributor.author","Stark, Holger"],["dc.contributor.author","Goeringer, H. Ulrich"],["dc.date.accessioned","2018-11-07T08:31:37Z"],["dc.date.available","2018-11-07T08:31:37Z"],["dc.date.issued","2009"],["dc.description.abstract","Mitochondrial pre-messenger RNAs in kinetoplastid protozoa are substrates of uridylate-specific RNA editing. RNA editing converts non-functional pre-mRNAs into translatable molecules and can generate protein diversity by alternative editing. Although several editing complexes have been described, their structure and relationship is unknown. Here, we report the isolation of functionally active RNA editing complexes by a multistep purification procedure. We show that the endogenous isolates contain two subpopulations of similar to 20S and similar to 35-40S and present the three-dimensional structures of both complexes by electron microscopy. The similar to 35-40S complexes consist of a platform density packed against a semispherical element. The similar to 20S complexes are composed of two subdomains connected by an interface. The two particles are structurally related, and we show that RNA binding is a main determinant for the interconversion of the two complexes. The similar to 20S editosomes contain an RNA-binding site, which binds gRNA, pre-mRNA and gRNA/pre-mRNA hybrid molecules with nanomolar affinity. Variability analysis indicates that subsets of complexes lack or possess additional domains, suggesting binding sites for components. Together, a picture of the RNA editing machinery is provided."],["dc.identifier.doi","10.1038/emboj.2009.19"],["dc.identifier.isi","000264437300017"],["dc.identifier.pmid","19197238"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17164"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0261-4189"],["dc.title","Snapshots of the RNA editing machine in trypanosomes captured at different assembly stages in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","463"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Nature Structural & Molecular Biology"],["dc.bibliographiccitation.lastpage","468"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Boehringer, Daniel"],["dc.contributor.author","Makarov, Evgeny M."],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Makarova, Olga V."],["dc.contributor.author","Kastner, Berthold"],["dc.contributor.author","Lührmann, Reinhard"],["dc.contributor.author","Stark, Holger"],["dc.date.accessioned","2021-03-05T08:58:30Z"],["dc.date.available","2021-03-05T08:58:30Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1038/nsmb761"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80158"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.eissn","1545-9985"],["dc.relation.issn","1545-9993"],["dc.title","Three-dimensional structure of a pre-catalytic human spliceosomal complex B"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","53"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","55"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Kastner, Berthold"],["dc.contributor.author","Fischer, Niels"],["dc.contributor.author","Golas, Monika Mariola"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Dube, Prakash"],["dc.contributor.author","Boehringer, Daniel"],["dc.contributor.author","Hartmuth, Klaus"],["dc.contributor.author","Deckert, Jochen"],["dc.contributor.author","Hauer, Florian"],["dc.contributor.author","Wolf, Elmar"],["dc.contributor.author","Uchtenhagen, Hannes"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Herzog, Franz"],["dc.contributor.author","Peters, Jan Michael"],["dc.contributor.author","Poerschke, Dietmar"],["dc.contributor.author","Lührmann, Reinhard"],["dc.contributor.author","Stark, Holger"],["dc.date.accessioned","2021-03-05T08:58:29Z"],["dc.date.available","2021-03-05T08:58:29Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1038/nmeth1139"],["dc.identifier.pii","BFnmeth1139"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80155"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.eissn","1548-7105"],["dc.relation.issn","1548-7091"],["dc.title","GraFix: sample preparation for single-particle electron cryomicroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","497"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cell"],["dc.bibliographiccitation.lastpage","509"],["dc.bibliographiccitation.volume","135"],["dc.contributor.author","Chari, Ashwin"],["dc.contributor.author","Golas, Monika M."],["dc.contributor.author","Klingenhäger, Michael"],["dc.contributor.author","Neuenkirchen, Nils"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Englbrecht, Clemens"],["dc.contributor.author","Sickmann, Albert"],["dc.contributor.author","Stark, Holger"],["dc.contributor.author","Fischer, Utz"],["dc.date.accessioned","2021-03-05T08:58:04Z"],["dc.date.available","2021-03-05T08:58:04Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1016/j.cell.2008.09.020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79997"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","0092-8674"],["dc.title","An Assembly Chaperone Collaborates with the SMN Complex to Generate Spliceosomal SnRNPs"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","869"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","883"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Golas, Monika M."],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Will, Cindy L."],["dc.contributor.author","Lührmann, Reinhard"],["dc.contributor.author","Stark, Holger"],["dc.date.accessioned","2021-03-05T08:58:09Z"],["dc.date.available","2021-03-05T08:58:09Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1016/j.molcel.2005.02.016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80024"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1097-2765"],["dc.title","Major Conformational Change in the Complex SF3b upon Integration into the Spliceosomal U11/U12 di-snRNP as Revealed by Electron Cryomicroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","493"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Molecular Therapy"],["dc.bibliographiccitation.lastpage","501"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Rudolph, Carsten"],["dc.contributor.author","Schillinger, Ulrike"],["dc.contributor.author","Ortiz, Aurora"],["dc.contributor.author","Plank, Christian"],["dc.contributor.author","Golas, Monika M."],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Stark, Holger"],["dc.contributor.author","Rosenecker, Joseph"],["dc.date.accessioned","2021-03-05T08:58:13Z"],["dc.date.available","2021-03-05T08:58:13Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1016/j.ymthe.2005.03.002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80046"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1525-0016"],["dc.title","Aerosolized nanogram quantities of plasmid DNA mediate highly efficient gene delivery to mouse airway epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","867"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","879"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Dube, Prakash"],["dc.contributor.author","Herzog, Franz"],["dc.contributor.author","Gieffers, Christian"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Riedel, Dietmar"],["dc.contributor.author","Müller, Shirley A."],["dc.contributor.author","Engel, Andreas"],["dc.contributor.author","Peters, Jan-Michael"],["dc.contributor.author","Stark, Holger"],["dc.date.accessioned","2021-03-05T08:58:09Z"],["dc.date.available","2021-03-05T08:58:09Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1016/j.molcel.2005.11.008"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80025"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1097-2765"],["dc.title","Localization of the Coactivator Cdh1 and the Cullin Subunit Apc2 in a Cryo-Electron Microscopy Model of Vertebrate APC/C"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1206"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Structural & Molecular Biology"],["dc.bibliographiccitation.lastpage","1212"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Häcker, Irina"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Golas, Monika M."],["dc.contributor.author","Wolf, Elmar"],["dc.contributor.author","Karagöz, Elif"],["dc.contributor.author","Kastner, Berthold"],["dc.contributor.author","Stark, Holger"],["dc.contributor.author","Fabrizio, Patrizia"],["dc.contributor.author","Lührmann, Reinhard"],["dc.date.accessioned","2018-11-07T11:09:41Z"],["dc.date.available","2018-11-07T11:09:41Z"],["dc.date.issued","2008"],["dc.description.abstract","The U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP) is a major, evolutionarily highly conserved spliceosome subunit. Unwinding of its U4/U6 snRNA duplex is a central event of spliceosome activation that requires several components of the U5 portion of the tri-snRNP, including the RNA helicase Brr2, Prp8 and the GTPase Snu114. Here we report the EM projection structure of the Saccharomyces cerevisiae tri-snRNP. It shows a modular organization comprising three extruding domains that contact one another in its central portion. We have visualized genetically tagged tri-snRNP proteins by EM and show here that U4/U6 snRNP forms a domain termed the arm. Conversely, a separate head domain adjacent to the arm harbors Brr2, whereas Prp8 and the GTPase Snu114 are located centrally. The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation."],["dc.identifier.doi","10.1038/nsmb.1506"],["dc.identifier.isi","000260638500019"],["dc.identifier.pmid","18953335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53061"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","1545-9985"],["dc.title","Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","73"],["dc.bibliographiccitation.issue","7243"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","U75"],["dc.bibliographiccitation.volume","459"],["dc.contributor.author","Andersen, Ebbe S."],["dc.contributor.author","Dong, Mingdong"],["dc.contributor.author","Nielsen, Morten M."],["dc.contributor.author","Jahn, Kasper"],["dc.contributor.author","Subramani, Ramesh"],["dc.contributor.author","Mamdouh, Wael"],["dc.contributor.author","Golas, Mariola Monika"],["dc.contributor.author","Sander, Bjoern"],["dc.contributor.author","Stark, Holger"],["dc.contributor.author","Oliveira, Cristiano L. P."],["dc.contributor.author","Pedersen, Jan Skov"],["dc.contributor.author","Birkedal, Victoria"],["dc.contributor.author","Besenbacher, Flemming"],["dc.contributor.author","Gothelf, Kurt V."],["dc.contributor.author","Kjems, Jorgen"],["dc.date.accessioned","2018-11-07T08:29:58Z"],["dc.date.available","2018-11-07T08:29:58Z"],["dc.date.issued","2009"],["dc.description.abstract","The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach(1). Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures(2-8). Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape(9) that can be used as platforms to arrange nanomaterials with high precision and specificity(9-13). A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures(14,15). Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm(3) in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos."],["dc.identifier.doi","10.1038/nature07971"],["dc.identifier.isi","000265801300031"],["dc.identifier.pmid","19424153"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16783"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0028-0836"],["dc.title","Self-assembly of a nanoscale DNA box with a controllable lid"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]